Evidence of random structural features in the heparin polymer.

Abstract:

:The first use of computer-simulation studies to examine heparin's structure has been reported. The product distributions obtained when porcine mucosal heparins were depolymerized with heparinase have been compared to computer-simulated distributions. The modeled distribution was relatively unaffected by the polydispersity and molecular weight of heparin. However, the percent of heparinase-cleavable glycosidic linkages and their distribution throughout the polymer resulted in a marked change in the simulated product distribution. The similarity between experimentally observed and computer-simulated product distributions is consistent with the random distribution of heparinase-cleavable sites in porcine mucosal heparin. Finally, a random distribution of N-acetyl residues with respect to heparinase-cleavable sites was experimentally observed.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Linhardt RJ,Merchant ZM,Rice KG,Kim YS,Fitzgerald GL,Grant AC,Langer R

doi

10.1021/bi00347a045

subject

Has Abstract

pub_date

1985-12-17 00:00:00

pages

7805-10

issue

26

eissn

0006-2960

issn

1520-4995

journal_volume

24

pub_type

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