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Stabilizing and Understanding a Miniprotein by Rational Redesign.

Abstract：

:Miniproteins reduce the complexity of the protein-folding problem allowing systematic studies of contributions to protein folding and stabilization. Here, we describe the rational redesign of a miniprotein, PPα, comprising a polyproline II helix, a loop, and an α helix. The redesign provides a de novo framework for interrogating noncovalent interactions. Optimized PPα has significantly improved thermal stability with a midpoint unfolding temperature (TM) of 51 °C. Its nuclear magnetic resonance structure indicates a density of stabilizing noncovalent interactions that is higher than that of the parent peptide, specifically an increased number of CH-π interactions. In part, we attribute this to improved long-range electrostatic interactions between the two helical elements. We probe further sequence-stability relationships in the miniprotein through a series of rational mutations.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Porter Goff KL,Nicol D,Williams C,Crump MP,Zieleniewski F,Samphire JL,Baker EG,Woolfson DN

doi

10.1021/acs.biochem.9b00067

subject

Has Abstract

pub_date

2019-07-16 00:00:00

pages

3060-3064

issue

28

eissn

0006-2960

issn

1520-4995

journal_volume

58

pub_type

杂志文章

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