Partial purification of Pde1 from Saccharomyces cerevisiae: enzymatic redundancy for the repair of 3'-terminal DNA lesions and abasic sites in yeast.

abstract：:The early steps of the proposed mechanistic pathway for dehydroquinate synthase have been probed with a series of substrate analogues. These analogues, 3-9, are structurally prohibited from undergoing the beta-elimination of inorganic phosphate that represents the committed step in the conversion of the substrate 3-de...

journal_title：Biochemistry

authors： Bender SL,Widlanski T,Knowles JR

更新日期：1989-09-19 00:00:00

abstract：:The importance of DNA polymerase-DNA minor groove interactions on translesion synthesis (TLS) was examined in vitro using variants of exonuclease-deficient Klenow polymerase and site-specifically modified DNA oligonucleotides. Polymerase variant R668A lacks primer strand interactions, while variant Q849A lacks templat...

journal_title：Biochemistry

authors： Gestl EE,Eckert KA

更新日期：2005-05-10 00:00:00

abstract：:A faithful transcription system for ribosomal RNA genes has been developed by using components from the small free-living amoeba Acanthamoeba castellanii. The system utilizes protein-free recombinant DNA as a template and in addition requires a crude cell-free extract containing RNA polymerase I and a transcription in...

journal_title：Biochemistry

authors： Paule MR,Iida CT,Perna PJ,Harris GH,Brown Shimer SL,Kownin P

更新日期：1984-08-28 00:00:00

abstract：:Myo1c is an unconventional myosin involved in cell signaling and membrane dynamics. Calcium binding to the regulatory-domain-associated calmodulin affects myo1c motor properties, but the kinetic details of this regulation are not fully understood. We performed actin gliding assays, ATPase measurements, fluorescence sp...

journal_title：Biochemistry

authors： Manceva S,Lin T,Pham H,Lewis JH,Goldman YE,Ostap EM

更新日期：2007-10-23 00:00:00

abstract：:Purple membranes of Halobacterium halobium were studied by differential scanning calorimetry. No transition was detected at temperatures below 70 degrees C. A small endothermic transition was seen at about 80 degrees C and a larger one at 100 degrees C. The larger transition is the irreversible denaturation of bacteri...

journal_title：Biochemistry

authors： Jackson MB,Sturtevant JM

更新日期：1978-03-07 00:00:00

abstract：:The cyclic decapeptide antibiotic tyrocidine A was studied by two relatively new methods, viz., correlation proton magnetic resonance (pmr) spectroscopy and double-resonance difference pmr spectroscopy. The correlation method of spectral accumulation provided pmr spectra of good resolution, and in addition the signal-...

journal_title：Biochemistry

authors： Gibbons WA,Beyer CF,Dadok J,Sprecher RF,Wyssbrod HR

更新日期：1975-01-28 00:00:00

abstract：:Expression of the PSR132 protein from Dianthus caryophyllus (carnation, clover pink) is induced in response to ethylene production associated with petal senescence, and thus the protein is named petal death protein (PDP). Recent work has established that despite the annotation of PDP in sequence databases as carboxyph...

journal_title：Biochemistry

authors： Teplyakov A,Liu S,Lu Z,Howard A,Dunaway-Mariano D,Herzberg O

更新日期：2005-12-20 00:00:00

abstract：:The homogeneous, single-walled phosphatidylcholine-cholesterol mixed vesicles were prepared by ultrasonic irradiation of egg phosphatidylcholine in the presence of various amounts of cholesterol in solution at 4 degrees under a nitrogen atmosphere followed by molecular sieve chromatography on a Sepharose 4B column. Ph...

journal_title：Biochemistry

authors： Newman GC,Huang C

更新日期：1975-07-29 00:00:00

abstract：:Oxidized cytochrome c oxidase exists in two reasonably well-defined conformations, a high-spin conformation with maximal absorption at 418 nm and a low-spin conformation with maximal absorption at 428 nm. The equilibrium between these two conformations has been studied as a function of pH, pressure, and temperature. p...

journal_title：Biochemistry

authors： Kornblatt JA,Hui Bon Hoa G

更新日期：1982-10-26 00:00:00

abstract：:Subunit II of the aa3 type cytochrome c oxidase contains a binuclear copper center (CuA) which functions as the entry point for electrons donated by cytochrome c. We have introduced site-specific mutations in residues liganding the CuA center in the oxidase of the bacterium Paracoccus denitrificans; the purified, full...

journal_title：Biochemistry

authors： Zickermann V,Wittershagen A,Kolbesen BO,Ludwig B

更新日期：1997-03-18 00:00:00

abstract：:S-Adenosylmethionine (SAM+) serves as the principal methylating agent in biological systems, but the thermodynamic basis of its reactivity does not seem to have been clearly established. Here, we show that methionine, methanol, and H+ combine to form S-methylmethionine (SMM+) with a temperature-independent equilibrium...

journal_title：Biochemistry

authors： Lewis CA Jr,Wolfenden R

更新日期：2018-07-03 00:00:00

abstract：:We have developed a system for targeting foreign DNA to hepatocytes in vitro using a soluble DNA carrier that takes advantage of receptor-mediated endocytosis to achieve internalization. The idea is based on the fact that hepatocytes possess a unique receptor that binds and internalizes galactose-terminal (asialo)glyc...

journal_title：Biochemistry

authors： Wu GY,Wu CH

更新日期：1988-02-09 00:00:00

abstract：:Alzheimer's disease (AD) paired helical filaments (PHFs), building blocks of neurofibrillary tangles (NFTs) are composed of hyperphosphorylated forms of the microtubule-associated protein tau (i.e., PHF-tau). Currently, much effort is devoted to the development of diagnostic antibodies specific for PHF-tau since eleva...

journal_title：Biochemistry

authors： Hoffmann R,Lee VM,Leight S,Varga I,Otvos L Jr

更新日期：1997-07-01 00:00:00

abstract：:Type II topoisomerases are responsible for DNA unlinking during DNA replication and chromosome segregation. Although eukaryotic enzymes are homodimers and prokaryotic enzymes are heterotetramers, both prokaryotic and eukaryotic type II topoisomerases belong to a single protein family. The amino- and carboxyl-terminal ...

journal_title：Biochemistry

authors： Lavasani LS,Hiasa H

更新日期：2001-07-24 00:00:00

abstract：:The calcium-dependent interaction of several annexins with membranes was studied. A novel technique was developed that allowed estimation of calcium binding to aggregating systems. This consisted of immobilized phospholipids on phenyl-Sepharose. Proteins associated with this affinity gel in the presence of radioactive...

journal_title：Biochemistry

authors： Evans TC Jr,Nelsestuen GL

更新日期：1994-11-15 00:00:00

abstract：:[15N]Glycine was biosynthetically incorporated into normal cellular N-ras p21 and a position 12 transforming mutant, in order to produce p21 proteins containing several site-specific NMR probes at or near activating positions in the guanine nucleotide binding domain. We have previously assigned all five glycine resona...

journal_title：Biochemistry

authors： Campbell-Burk S

更新日期：1989-11-28 00:00:00

abstract：:The Bacillus subtilis DNA-dependent RNA polymerase holoenzyme and core enzyme each contain approximately two atoms of zinc per molecule. When the dissociated subunits of the enzyme are passed through a blue dextran-Sepharose affinity column, only the beta subunit binds to the column. The total zinc content of the enzy...

journal_title：Biochemistry

authors： Halling SM,Sanchez-Anzaldo FJ,Fukuda R,Doi RH,Meares CF

更新日期：1977-06-28 00:00:00

abstract：:The apolipoproteins of insect lipophorin were dissociated in guanidinium chloride and isolated by gel permeation chromatography. Over 98% of the total lipid in lipophorin was associated with apolipophorin I (apoLp-I), thus suggesting this apolipoprotein to be the lipid binding component of the particle. ApoLp-I was de...

journal_title：Biochemistry

authors： Kawooya JK,Wells MA,Law JH

更新日期：1989-08-08 00:00:00

abstract：:An affinity labeling technique has been developed to identify the folate-methotrexate transporter of Leishmania donovani promastigotes using "activated" derivatives of the ligands. These "activated" derivatives were synthesized by incubating folate and methotrexate with a 10-fold excess of 1-ethyl-3-[3-(dimethylamino)...

journal_title：Biochemistry

authors： Beck JT,Ullman B

更新日期：1989-08-22 00:00:00

abstract：:Rat brain and kidney cDNA libraries were constructed and screened with a cDNA insert corresponding to the mRNA for the sheep kidney Na+,K+-ATPase catalytic subunit. The alpha-subunit cDNAs isolated from the kidney library were derived from a single class of messenger RNA, and the brain cDNAs were derived from three cl...

journal_title：Biochemistry

authors： Shull GE,Greeb J,Lingrel JB

更新日期：1986-12-16 00:00:00

abstract：:The traditional approach to studying protein folding involves applying a perturbation, usually denaturant or mutation, and determining the effect upon the free energy of folding, DeltaG0, and the activation free energy, DeltaG(not equal). Data collected as a function of the perturbation can be used to construct rate e...

journal_title：Biochemistry

authors： Song B,Cho JH,Raleigh DP

更新日期：2007-12-11 00:00:00

abstract：:Membrane-bound phosphodiesterase 6 (PDE6) plays an important role in visual signal transduction by regulating cGMP levels in rod photoreceptor cells. Our understanding of PDE6 catalysis and structure suffers from inadequate characterization of the α and β subunit catalytic core, interactions of the core with two intri...

journal_title：Biochemistry

authors： Baker BY,Palczewski K

更新日期：2011-11-08 00:00:00

abstract：:The dynamics of the enthalpy and volume changes found in the photodissociation of CO from sperm whale carboxymyoglobin and two site-directed mutants in which arginine-45 is replaced by glycine and asparagine are examined by photoacoustic calorimetry. An intermediate is observed whose lifetime at 20 degrees C is 700 ns...

journal_title：Biochemistry

authors： Westrick JA,Peters KS,Ropp JD,Sligar SG

更新日期：1990-07-17 00:00:00

abstract：:Cytochrome b(561) in adrenal chromaffin vesicle membranes conveys electron equivalents from extravesicular ascorbate to the intravesicular monodehydroascorbate radical. We conducted a stopped-flow study on the reaction of ascorbate with purified cytochrome b(561) in the detergent-solubilized state for the first time. ...

journal_title：Biochemistry

authors： Takigami T,Takeuchi F,Nakagawa M,Hase T,Tsubaki M

更新日期：2003-07-15 00:00:00

abstract：:The relative roles of insulin and glucose in the regulation of hepatic glycogen synthase and phosphorylase were studied in hepatocytes from fed rats. Elevation of extra-cellular glucose led to a rapid decrease in phosphorylase a activity followed by a slower increase in glycogen synthase I activity. A reciprocal and c...

journal_title：Biochemistry

authors： Witters LA,Avruch J

更新日期：1978-02-07 00:00:00

abstract：:We have investigated the interaction of the bisbenzimidazole derivative Hoechst 33258 with the self-complementary dodecadeoxynucleotide duplex d(GTGGAATTCCAC)2 using one-dimensional (1D) and two-dimensional (2D) proton nuclear magnetic resonance (1H NMR) spectroscopy. To monitor the extent of complex formation, we use...

journal_title：Biochemistry

authors： Fede A,Labhardt A,Bannwarth W,Leupin W

更新日期：1991-12-03 00:00:00

abstract：:The reaction between chymotrypsin and N-acetyl-L-phenylalanine p-nitroanilide has been studied at subzero temperatures in fluid aqueous dimethyl sulfoxide solvent. Following initiation of the reaction at temperatures as low as -90 degrees C, a series of four reactions prior to the normal rate-limiting step (acylation)...

journal_title：Biochemistry

authors： Fink AL

更新日期：1976-04-06 00:00:00

abstract：:The EcDos protein belongs to a group of heme-based sensors that detect their ligands with a heme-binding PAS domain. Among these various heme-PAS proteins, EcDos is unique in having its heme iron coordinated at both axial positions to residues of the protein. To achieve its high affinities for ligands, one of the axia...

journal_title：Biochemistry

authors： Gonzalez G,Dioum EM,Bertolucci CM,Tomita T,Ikeda-Saito M,Cheesman MR,Watmough NJ,Gilles-Gonzalez MA

更新日期：2002-07-02 00:00:00

abstract：:A metabolite of vitamin D has been isolated in pure form from incubation of 25-hydroxyvitamin D3 with embryonic chick calvarial cells that had been grown on Cytodex 1 microcarrier beads. The isolation involved dichloromethane extraction of the cells and incubation medium, followed by Sephadex LH-20 column chromatograp...

journal_title：Biochemistry

authors： Turner RT,Howard GA,Puzas JE,Baylink DJ,Knapp DR

更新日期：1983-03-01 00:00:00

abstract：:We have investigated the loss of histone H1 from chromatin [D'Anna, J. A., Gurley, L. R., & Tobey, R. A. (1982) Biochemistry 21, 3991-4001] and the structure of chromatin from Chinese hamster (line CHO) cells blocked in early S phase by sequential use of isoleucine deprivation G1 block and 1 mM hydroxyurea (HU) blocka...

journal_title：Biochemistry

authors： D'Anna JA,Prentice DA

更新日期：1983-11-22 00:00:00