Chromatin condensing functions of the linker histone C-terminal domain are mediated by specific amino acid composition and intrinsic protein disorder.

abstract：:In experiments designed to characterize the basis of amyloid fibril stability through mutational analysis of the Abeta (1-40) molecule, fibrils exhibit consistent, significant structural malleability. In these results, and in other properties, amyloid fibrils appear to more resemble plastic materials generated from sy...

journal_title：Biochemistry

authors： Wetzel R,Shivaprasad S,Williams AD

更新日期：2007-01-09 00:00:00

abstract：:The enzyme As(III) S-adenosylmethionine methyltransferase (EC 2.1.1.137) (ArsM or AS3MT) is found in members of every kingdom, from bacteria to humans. In these enzymes, there are three conserved cysteine residues at positions 72, 174, and 224 in the CmArsM orthologue from the thermophilic eukaryotic alga Cyanidioschy...

journal_title：Biochemistry

authors： Marapakala K,Qin J,Rosen BP

更新日期：2012-02-07 00:00:00

abstract：:Illumination of envelope vesicles prepared from Halobacterium halobium cells causes translocation of protons from inside to outside, due to the light-induced cycling of bacteriorhodopsin. This process results in a pH gradient across the membranes, an electrical potential, and the movements of K+ and Na+. The electrica...

journal_title：Biochemistry

authors： Renthal R,Lanyi JK

更新日期：1976-05-18 00:00:00

abstract：:Crowding, which characterizes the interior of all living cells, has been shown to dramatically affect biochemical processes, leading to stabilization of compact morphologies, enhanced macromolecular associations, and altered reaction rates. Due to the crowding-mediated shift in binding equilibria toward association, c...

journal_title：Biochemistry

authors： Goobes R,Kahana N,Cohen O,Minsky A

更新日期：2003-03-04 00:00:00

abstract：:The solution structure of the C-terminal fragment 255-316 of thermolysin has been determined by two-dimensional proton NMR methods. For this disulfide-free fragment there was a previous proposal according to which it would fold into a stable helical structure forming a dimer at concentrations above 0.06 mM. A complete...

journal_title：Biochemistry

authors： Rico M,Jiménez MA,González C,De Filippis V,Fontana A

更新日期：1994-12-13 00:00:00

abstract：:Sedimentation in a preparative ultracentrifuge was used to determine the affinity of heavy meromyosin, HMM, for regulated actin, F-actin plus troponin-tropomyosin, in the presence of MgATP at pH 7.0, 20 degrees C, and mu = 18 mM. HMM was prepared from vertebrate striated muscle myosin by a mild chymotryptic digestion....

journal_title：Biochemistry

authors： Wagner PD,Stone DB

更新日期：1983-03-15 00:00:00

abstract：:3-Deoxy-d- manno-2-octulosonate-8-phosphate (KDO8P) synthase catalyzes the first step of lipopolysaccharide biosynthesis, namely condensation of phosphoenolpyruvate (PEP) with arabinose 5-phosphate (A5P), to produce KDO8P. We have characterized Campylobacter jejuni KDO8P synthase and its inhibition by KDO8P oxime. It ...

journal_title：Biochemistry

authors： Gama SR,Balachandran N,Berti PJ

更新日期：2018-09-11 00:00:00

abstract：:Urea lesions are formed in DNA because of free radical damage of the thymine base, and their occurrence in DNA blocks DNA polymerases, which has deleterious consequences. Recently, it has been shown that urea is capable of forming hydrogen bonding and stacking interactions with nucleobases, which are responsible for t...

journal_title：Biochemistry

authors： Suresh G,Padhi S,Patil I,Priyakumar UD

更新日期：2016-10-11 00:00:00

abstract：:It has been suggested that the down-regulation of hepatic HMG-CoA reductase by dietary cholesterol requires modification of the cholesterol molecule before it can exert its suppressive action. In a recent study [Lund, E., Breuer, O., & Björkhem, I. (1992) J. Biol. Chem. 267, 25092-25097], we showed that side-chain hyd...

journal_title：Biochemistry

authors： Lund E,Björkhem I

更新日期：1994-01-11 00:00:00

abstract：:The enzyme thymidylate synthase (TS) catalyzes a complex reaction that involves forming and breaking at least six covalent bonds. The physical nature of the hydride transfer step in this complex reaction cascade has been studied by means of isotope effects and their temperature dependence. Competitive kinetic isotope ...

journal_title：Biochemistry

authors： Agrawal N,Hong B,Mihai C,Kohen A

更新日期：2004-02-24 00:00:00

abstract：:Tk-subtilisin from the hyperthermophiolic archaeon Thermococcus kodakaraensis matures from Pro-Tk-subtilisin upon autoprocessing and degradation of Tk-propeptide [Tanaka, S., Saito, K., Chon, H., Matsumura, H., Koga, Y., Takano, K., and Kanaya, S. (2007) J. Biol. Chem. 282, 8246-8255]. It requires Ca(2+) for folding a...

journal_title：Biochemistry

authors： Takeuchi Y,Tanaka S,Matsumura H,Koga Y,Takano K,Kanaya S

更新日期：2009-11-10 00:00:00

abstract：:The third intracellular loop (IL3) of G protein-coupled receptors (GPCRs) is an important contact domain between GPCRs and their G proteins. Previously, the IL3 of Ste2p, a Saccharomyces cerevisiae GPCR, was suggested to undergo a conformational change upon activation as detected by differential protease susceptibilit...

journal_title：Biochemistry

authors： Umanah GK,Huang LY,Maccarone JM,Naider F,Becker JM

更新日期：2011-08-16 00:00:00

abstract：:To date, little work has been conducted on the relationship between solute and buffer molecules and conformational exchange motion in enzymes. This study uses solution NMR to examine the effects of phosphate, sulfate, and acetate in comparison to MES- and HEPES-buffered references on the chemical shift perturbation an...

journal_title：Biochemistry

authors： Wong M,Khirich G,Loria JP

更新日期：2013-09-17 00:00:00

abstract：:Calcium protects a limited number of embryonic chick neural retina cell surface proteins from tryptic cleavage. One glycoprotein of Mr approximately 1.3 X 10(5) and pI approximately 4.8 (gp130) is present in intact retinas and, in the presence of Ca2+, is both resistant to tryptic cleavage and poorly iodinated. When c...

journal_title：Biochemistry

authors： Cook JH,Pratt RS,Lilien J

更新日期：1984-02-28 00:00:00

abstract：:Using cysteine mutagenesis and chemical modification by methanethiosulfonate derivatives, it was demonstrated that the external putative loop, joining transmembrane segments (TM's) IV-V of rabbit Na+/glucose cotransporter, rSGLT1, forms part of a Na+ binding and voltage sensing domain. Within this region, exposure to ...

journal_title：Biochemistry

authors： Huntley SA,Krofchick D,Silverman M

更新日期：2006-04-11 00:00:00

abstract：:6-Hydroxymethyl-7,8-dihydropterin pyrophosphokinase (HPPK) catalyzes the pyrophosphoryl transfer from ATP to 6-hydroxymethyl-7,8-dihydropterin (HP), the first reaction in the folate biosynthetic pathway. Arginine residues 82 and 92, strictly conserved in 35 HPPK sequences, play dynamic roles in the catalytic cycle of ...

journal_title：Biochemistry

authors： Blaszczyk J,Li Y,Shi G,Yan H,Ji X

更新日期：2003-02-18 00:00:00

abstract：:Beta-lactamases are the most widespread resistance mechanism to beta-lactam antibiotics, such as the penicillins and cephalosporins. Transition-state analogues that bind to the enzymes with nanomolar affinities have been introduced in an effort to reverse the resistance conferred by these enzymes. To understand the or...

journal_title：Biochemistry

authors： Roth TA,Minasov G,Morandi S,Prati F,Shoichet BK

更新日期：2003-12-16 00:00:00

abstract：:The members of the glutamine amidotransferase (GATase) family catalyze the incorporation of ammonia within numerous metabolic pathways and can be categorized in two classes. Here, we concentrated on class I GATases, which are heteromeric enzyme complexes consisting of synthase subunits and glutaminase subunits with a ...

journal_title：Biochemistry

authors： Semmelmann F,Hupfeld E,Heizinger L,Merkl R,Sterner R

更新日期：2019-06-04 00:00:00

abstract：:Anticoagulation factor II (ACF II) isolated from the venom of Agkistrodon acutus is an activated coagulation factor X-binding protein in a Ca(2+)-dependent fashion with marked anticoagulant activity. The equilibrium unfolding/refolding of apo-ACF II, holo-ACF II, and Tb(3+)-reconstituted ACF II in guanidine hydrochlor...

journal_title：Biochemistry

authors： Xu X,Liu Q,Xie Y

更新日期：2002-03-19 00:00:00

abstract：:Chymopapain, a cysteine protease of papaya latex, has been purified with the use of fast protein liquid chromatography. Two homogeneous fractions were analyzed for thiol content and thiol reactivity. It was found that peak 1 and peak 2 contained two and three thiol groups, respectively, per mole of enzyme. This result...

journal_title：Biochemistry

authors： Kóródi I,Asbóth B,Polgár L

更新日期：1986-11-04 00:00:00

abstract：:The thermal dependence of the structural stability of membrane-reconstituted yeast cytochrome c oxidase has been studied by using different techniques including high-sensitivity differential scanning calorimetry, differential detergent solubility thermal gel analysis, and enzyme activity measurements. For these studie...

journal_title：Biochemistry

authors： Morin PE,Diggs D,Freire E

更新日期：1990-01-23 00:00:00

abstract：:The recognition sequence of the Myb protein has been recently described to be pyAACKGHH (where py = T/C, K = G/T, and H = A/C/T), modifying the earlier identification as pyAACKG [Ording, E., et al. (1994) Eur. J. Biochem. 222, 113-120]. We had earlier determined the solution structure of the minimal cognate sequence T...

journal_title：Biochemistry

authors： Radha PK,Patel PK,Hosur RV

更新日期：1998-07-14 00:00:00

abstract：:At any instant, the human erythrocyte sugar transporter presents at least one sugar export site but multiple sugar import sites. The present study asks whether the transporter also presents more than one sugar exit site. We approached this question by analysis of binding of [3H]cytochalasin B (an export conformer liga...

journal_title：Biochemistry

authors： Cloherty EK,Levine KB,Carruthers A

更新日期：2001-12-25 00:00:00

abstract：:The general view on Rhizobium chitolipooligosaccharides (CLOS) is that they are made in very low levels as diffusible molecules and are primarily secreted by the bacteria into the extracellular milieu where they interact with the host. However, the structural and predicted physicochemical properties of these amphiphil...

journal_title：Biochemistry

authors： Orgambide GG,Lee J,Hollingsworth RI,Dazzo FB

更新日期：1995-03-21 00:00:00

abstract：:In bacteria, the transcription of virulence genes is usually controlled by a cell density-dependent process known as "quorum sensing" (QS). QS relies on small diffusible signaling molecules that cross the bacterial cell wall and activate target transcription factors after a threshold concentration has been reached. Be...

journal_title：Biochemistry

authors： Yu S,Jensen V,Seeliger J,Feldmann I,Weber S,Schleicher E,Häussler S,Blankenfeldt W

更新日期：2009-11-03 00:00:00

abstract：:Phage display technique is a powerful tool with which to identify novel binding sequences for antibody and receptor targets. Few studies, however, have used this technology to select affinity peptides for ligand molecules. Here, we screened a peptide phage library for binding to toxic shock syndrome toxin 1 (TSST-1) t...

journal_title：Biochemistry

authors： Sato A,Ida N,Fukuyama M,Miwa K,Kazami J,Nakamura H

更新日期：1996-08-13 00:00:00

abstract：:The only identified component of the scrapie prion is PrPSc, a glycosylinositol phospholipid (GPI)-linked protein that is derived from the cellular isoform (PrPC) by an as yet unknown posttranslational event. Analysis of the PrPSc GPI has revealed six different glycoforms, three of which are unprecedented. Two of the ...

journal_title：Biochemistry

authors： Stahl N,Baldwin MA,Hecker R,Pan KM,Burlingame AL,Prusiner SB

更新日期：1992-06-02 00:00:00

abstract：:The folate chromophore in native Escherichia coli DNA photolyase ([6R]-5,10-CH+-H4Pte(Glu)n=3-6) serves as an antenna, transferring light energy to the fully reduced flavin (FADH2) reaction center at high efficiency (EET = 0.92). Apophotolyase reconstituted after an overnight incubation with [6R,S]-5,10-CH+-H4folate (...

journal_title：Biochemistry

authors： Lipman RS,Jorns MS

更新日期：1996-06-18 00:00:00

abstract：:Among hormone-inducible transcription factors, estrogen receptors (ERs) play important roles in tissue growth and differentiation, via either direct or indirect binding, in the nucleus, to specific DNA targets called estrogen responsive elements (EREs), or through nongenomic pathways. In humans, two estrogen receptor ...

journal_title：Biochemistry

authors： Le Grand A,André-Leroux G,Marteil G,Duval H,Sire O,Le Tilly V

更新日期：2015-06-30 00:00:00

abstract：:The differential scanning calorimetry trace of F1ATPase, prepared from beef heart submitochondrial particles, has a single sharp endothermic transition at 80.5 +/- 1.0 degrees C and a half-height peak width of 2.0 +/- 0.2 degrees. The transition enthalpy is 19 +/- 2 cal/g of protein. Submitochondrial particles (SMP) h...

journal_title：Biochemistry

authors： Kresheck GC,Adade AB,Vanderkooi G

更新日期：1985-03-26 00:00:00