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Campylobacter jejuni KDO8P Synthase, Its Inhibition by KDO8P Oxime, and Control of the Residence Time of Slow-Binding Inhibition.

Abstract：

:3-Deoxy-d- manno-2-octulosonate-8-phosphate (KDO8P) synthase catalyzes the first step of lipopolysaccharide biosynthesis, namely condensation of phosphoenolpyruvate (PEP) with arabinose 5-phosphate (A5P), to produce KDO8P. We have characterized Campylobacter jejuni KDO8P synthase and its inhibition by KDO8P oxime. It was metal-dependent and homotetrameric and followed a rapid equilibrium sequential ordered ter ter kinetic mechanism in which Mn2+ bound first, followed by PEP and then A5P. It was inhibited by KDO8P oxime, an analogue of 3-deoxy-d- arabino-heptulosonate 7-phosphate (DAHP) oxime, a transition-state mimic of DAHP synthase. Inhibition was uncompetitive-like with respect to Mn2+ and competitive with respect to PEP and A5P. It displayed both fast-binding inhibition ( Ki = 10 μM) and slow-binding inhibition ( Ki* = 0.57 μM). The residence times on the enzyme ( tR) ranged from 27 min in the absence of free inhibitor to 69 h with excess inhibitor. The dependence of tR on the free inhibitor concentration suggested intersubunit communication within the homotetramer between high- and low-affinity sites. This confirms the generality of the oxime functional group, a small, neutral phosphate bioisostere, as an α-carboxyketose synthase inhibitor and highlights the challenge that intersubunit communication poses to effective inhibition.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Gama SR,Balachandran N,Berti PJ

doi

10.1021/acs.biochem.8b00748

subject

Has Abstract

pub_date

2018-09-11 00:00:00

pages

5327-5338

issue

36

eissn

0006-2960

issn

1520-4995

journal_volume

57

pub_type

杂志文章

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