Abstract：
:The cooperative binding of the Escherichia coli DNA unwinding protein to single-stranded DNA has been studied by electron microscopy. Analysis of the electron microscopic data by means of a simple statistical mechanical model has yielded a value of 3.8-7.6 X 10(10) l./mol for the cooperative binding constant in 0.15 M NaCl. Studied under elevated salt conditions have shown that the average DNA protein complex length is 50% of the length found at 0.04 or 0.15 M NaCl.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Ruyechan WT,Wetmur JGdoi
10.1021/bi00696a023subject
Has Abstractpub_date
1975-12-16 00:00:00pages
5529-34issue
25eissn
0006-2960issn
1520-4995journal_volume
14pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The mechanism of activation of cGMP phosphodiesterase by the GTP-binding protein in the disc membrane of retinal rods has been investigated by measuring the light-induced phosphodiesterase activity in reconstituted systems where the concentration of either the GTP-binding protein or the phosphodiesterase is varied. Th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00444a040
更新日期：1989-09-05 00:00:00
abstract：:An overexpression system for spinach apocytochrome b(6) as a fusion protein to a maltose-binding protein in Escherichia coli was established using the expression vector pMalp2. The fusion of the cytochrome b(6) to the periplasmic maltose-binding protein directs the cytochrome on the Sec-dependent pathway. The cytochro...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi047422w
更新日期：2005-05-24 00:00:00
abstract：:The temperature dependence of the packing (order) and fluidity (microviscosity) of rabbit small, intestinal brush border vesicle membranes and of liposomes made from their extracted lipids has been investigated by using a variety of lipid spin probes. The lipids in the brush border membrane are present essentially as ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00265a036
更新日期：1982-10-26 00:00:00
abstract：:The interaction of the retroviral nucleocapsid protein (NC) with nucleic acids forms the basis of its varied roles in the replication cycle, which include binding and condensing the viral RNA within the virion, stimulation of the early steps in reverse transcription, and dissociation from RNA in the replication comple...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00126a020
更新日期：1992-03-24 00:00:00
abstract：:In prokaryotes, a large share of the proteins are secreted from the cell through a process that requires their translocation across the cytoplasmic membrane. This process is mediated by the universally conserved Sec system with homologues in the endoplasmic reticulum and thylakoid membranes of eukaryotes. The Sec syst...
journal_title：Biochemistry
pub_type： 杂志文章,评审
doi：10.1021/bi400913x
更新日期：2013-10-01 00:00:00
abstract：:The solution structure of the complementary d(C1-A2-T3-G4-X5-G6-T7-A8-C9).d(G10-T11-A12-C13-A14-C15-A16-T17-G18) DNA duplex (designated X.A 9-mer), which contains a 1,N2-propanodeoxyguanosine exocyclic adduct X5 opposite deoxyadenosine A14 at the center, is pH dependent [Kouchakdjian, M., Eisenberg, M., Live, D., Mari...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00066a004
更新日期：1993-04-20 00:00:00
abstract：:Theory for enzyme-catalyzed reactions is developed for the dependence on product concentration of deuterium isotope effects on V and V/K. Generally, a product that decreases the off-rate for a second product to zero causes the isotope effect on V/K to decrease to DKeq and that on V to decrease to a value between 1 and...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00058a013
更新日期：1993-02-23 00:00:00
abstract：:Many proteins that are destined to reside within the lumen of the peroxisome contain the peroxisomal targeting signal-1 (PTS1), a C-terminal tripeptide approximating the consensus sequence -Ser-Lys-Leu-COO(-). The PTS1 is recognized by the tetratricopeptide repeat (TPR) domains of PEX5, a cytosolic receptor that cycle...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi027034z
更新日期：2003-02-18 00:00:00
abstract：:The conformation of the glycerol group of a number of diacyl and monoacyl (lyso) phospholipids differing in the chemical nature of the head group was studied by 1H high-resolution NMR and X-ray crystallography. The NMR measurements were carried out with solutions or micellar dispersions of the lipids in deuteriated or...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00426a014
更新日期：1988-12-27 00:00:00
abstract：:Amphiphiles respond both to polar and to nonpolar solvents. In this paper X-ray diffraction and osmotic stress have been used to examine the phase behavior, the structural dimensions, and the work of deforming the monolayer-lined aqueous cavities formed by mixtures of dioleoylphosphatidylethanolamine (DOPE) and dioleo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00453a010
更新日期：1990-01-09 00:00:00
abstract：:Two-dimensional mapping of the tryptic phosphopeptides generated following in vitro protein kinase C phosphorylation of the myosin heavy chain isolated from human platelets and chicken intestinal epithelial cells shows a single radioactive peptide. These peptides were found to comigrate, suggesting that they were iden...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00218a012
更新日期：1991-01-29 00:00:00
abstract：:Cyclic AMP receptor protein is involved in the regulation of more than 20 genes. A step in the mechanism of activation of transcription is to induce a significant bending of the DNA upon complex formation between specific DNA and the protein. The induced DNA bending and a structure of the protein-DNA complex were stud...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00137a011
更新日期：1992-06-09 00:00:00
abstract：:The inhibition mechanism of serpins requires a change in structure to entrap the target proteinase as a stable acyl-enzyme complex. Although it has generally been assumed that reactive center loop insertion and associated conformational change proceeds in a concerted manner, this has not been demonstrated directly. Th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi025967p
更新日期：2002-10-08 00:00:00
abstract：:Fully functional variable fragments (Fv) of D1.3, a mouse antibody directed against the hen egg lysozyme, were readily produced as hybrids (Fv-MalE) with the maltose-binding protein of Escherichia coli and purified independently of their antigen-binding properties. We used site-directed mutations of residues in the co...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi961419y
更新日期：1997-01-07 00:00:00
abstract：:NSC 613862 (S)-(-) and NSC 613863 (R)-(+) are the two chiral isomers of ethyl-5-amino-2-methyl-1,2-dihydro-3-phenylpyrido[3, 4-b]pyrazin-7-yl carbamate. Both compounds bind to tubulin in a region that overlaps the colchicine site. They induce formation of abnormal polymers from purified GTP-Mg-tubulin, the active asse...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi970568t
更新日期：1998-01-13 00:00:00
abstract：:The phosphocarrier protein HPr of the phosphoenolpyruvate-dependent sugar transport system of Escherichia coli can exist in a phosphorylated and a nonphosphorylated form. During phosphorylation, the phosphoryl group is carried on a histidine residue, His15. The hydrogen-bonding state of this histidine was examined wit...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00487a026
更新日期：1990-09-04 00:00:00
abstract：:A bifunctional molecule was genetically engineered which contained an amino-terminal effector domain that bound immunoglobulin Fc (fragment B of staphylococcal protein A) and a carboxyl-terminal domain that bound digoxin [a single-chain Fv (sFv)]. Effector and sFv binding properties were virtually identical with those...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00487a005
更新日期：1990-09-04 00:00:00
abstract：:Complete assignments of nitrogen-15 resonances of gramicidin S have been made in dimethyl sulfoxide, trifluoroethanol, and in a solvent mixture of dimethyl sulfoxide (50%) and methanol (50%). The assignments are achieved by utilizing the secondary structure of gramicidin S, by comparing the nitrogen-15 spectrum of gra...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00606a005
更新日期：1978-06-27 00:00:00
abstract：:S-Adenosylmethionine decarboxylase is a pyruvate-dependent enzyme. The enzyme forms a Schiff base with substrate, S-adenosylmethionine, through the pyruvoyl moiety. This facilitates the release of CO2 from the substrate, which must then be protonated on the alpha carbon in order to permit hydrolysis of the Schiff base...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9825201
更新日期：1999-02-23 00:00:00
abstract：:The three-dimensional high-resolution structure of rhodopsin is unknown, as is the case for almost all integral membrane patients. As part of an alternative approach to determine of membrane protein structure, we are pursuing the structure of cytoplasmic domains of this G-protein receptor. A peptide, rhoIII, with the ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00045a002
更新日期：1995-11-14 00:00:00
abstract：:Glucarate dehydratase (GlucD) from Pseudomonas putida catalyzes the dehydration of both (D)-glucarate and (L)-idarate to 3-deoxy-(L)-threo-2-hexulosarate as well as their epimerization. (D)-[6-13C]Glucarate and (L)-[6-13C]idarate have been synthesized for use in continuous assay of the reactions catalyzed by GlucD by ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi981122v
更新日期：1998-10-13 00:00:00
abstract：:Benzoylformate decarboxylase (BFDC) and pyruvate decarboxylase (PDC) are both thiamin diphosphate-dependent enzymes. The two share a common three-dimensional structure and catalyze a similar chemical reaction, i.e., decarboxylation of 2-keto acids. However, they vary significantly in their substrate utilization patter...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9008402
更新日期：2009-09-08 00:00:00
abstract：:Cdc31, the Saccharomyces cerevisiae centrin, is an EF-hand calcium-binding protein essential for the cell division and mRNA nuclear export. We used biophysical techniques to investigate its calcium, magnesium, and protein target binding properties as well as their conformations in solution. We show here that Cdc31 dis...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi200518d
更新日期：2011-07-26 00:00:00
abstract：:In invertebrate photoreceptors, illuminated rhodopsin activates multiple G proteins, which are assumed to initiate multiple phototransduction cascades. In this paper, we focused on one of the phototransduction cascades, which utilizes rhodopsin, a Gq-like G protein, and phospholipase C (PLC). A Gq-like G protein from ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi961360v
更新日期：1996-12-10 00:00:00
abstract：:Reactive amyloidosis occurs during chronic inflammation and involves deposition of amyloid A (AA) fibrils in many organs. Amyloid A is derived by proteolysis from serum amyloid A component (SAA), a major acute-phase reactant in many species. Since spontaneous amyloidosis occurs commonly in Syrian hamsters, we have stu...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00437a040
更新日期：1989-05-30 00:00:00
abstract：:Trytophanase from Bacillus alvei was resolved from its coenzyme, pyridoxal phosphate, by treatment with cysteine followed by column chromatography. Spectrophotometric titration of apoenzyme with pyridoxal-P showed 1 mol of pyridoxal-P bound per 52,000 g of enzyme. Kinetic analysis of coenzyme binding showed hyperbolic...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00690a024
更新日期：1975-09-23 00:00:00
abstract：:Tryptic digestion studies of the human erythrocyte glucose carrier have shown that a reactive and transport-sensitive exofacial sulfhydryl is located in the carboxy-terminal half of the molecule, corresponding to Cys347, Cys421, or Cys429. In the present studies, the erythrocyte glucose carrier labeled on the exofacia...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00497a014
更新日期：1990-11-13 00:00:00
abstract：:Cytochrome P450BM3 is a bacterial enzyme with a heme cofactor that binds small diatomic ligands. Here we report the first study of carbon monoxide (CO) photodissociation and rebinding in ferrous P450BM3 on an ultrafast time scale. We monitored dissociation of carbon monoxide upon Soret band excitation using visible an...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi900351m
更新日期：2009-07-07 00:00:00
abstract：:Streptomyces griseus trypsin has been isolated from Pronase by ion-exchange chromatography on CM-Sephadex and SE-Sephadex. The isolated enzyme was homogeneous by the criteria tested except for a low degree of contamination by an enzyme with nontryptic activity. The latter could be partially resolved by chromatography ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00677a010
更新日期：1975-03-25 00:00:00
abstract：:The side chains of histidine and aspartate residues form a hydrogen bond in the active sites of many enzymes. In serine proteases, the His...Asp hydrogen bond of the catalytic triad is known to contribute greatly to catalysis, perhaps via the formation of a low-barrier hydrogen bond. In bovine pancreatic ribonuclease ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi972766q
更新日期：1998-06-23 00:00:00
