Abstract：
:A new quantitative technique for measuring the binding of proteins to membranes is described. The method is based on a combination of total internal reflection fluorescence microscopy and the preparation of supported planar bilayers. Specific and reversible binding of a fluorescence-labeled monoclonal antibody to lipid haptens that were embedded in supported bilayers has been measured by this technique and compared to binding experiments that were conducted on membrane vesicles in solution. Equilibrium binding constants and kinetic parameters have been determined and used to expand the picture of the antibody-lipid hapten reaction. Estimates demonstrate that this technique is capable of measuring a broad range of binding constants (down to about 10(4) M-1) using only small amounts of ligand and receptor.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Kalb E,Engel J,Tamm LKdoi
10.1021/bi00458a036subject
Has Abstractpub_date
1990-02-13 00:00:00pages
1607-13issue
6eissn
0006-2960issn
1520-4995journal_volume
29pub_type
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