Digestion of RNA of chromatin and nuclear ribonucleoprotein by staphylococcal nuclease.

abstract：:The preparation, spectroscopic investigation, structure determination, conformational analysis, and modeling of the Dewar pyrimidinone photoproduct of thymidylyl-(3'----5')-thymidine, previously referred to as TpT3 [Johns, H. E., Pearson, M. L., LeBlanc, J. C., & Heilleiner, C. W. (1964) J. Mol. Biol. 9, 503-524], is ...

journal_title：Biochemistry

authors： Taylor JS,Garrett DS,Cohrs MP

更新日期：1988-09-20 00:00:00

abstract：:There is considerable evidence that mammalian beta-tubulin is phosphorylated. Specifically, of the seven beta isotypes, the phosphorylated one is beta III, the isotype found almost entirely in neurons. The phosphate is added at a serine and perhaps a tyrosine near the C-terminus. All the evidence to date has been gath...

journal_title：Biochemistry

authors： Khan IA,Ludueña RF

更新日期：1996-03-26 00:00:00

abstract：:The solution conformation of potato carboxypeptidase inhibitor (CPI) has been investigated by 1H NMR spectroscopy. The spectrum is assigned in a sequential manner by using two-dimensional NMR techniques to identify through-bond and through-space (less than 5 A) connectivities. A set of 309 approximate interproton dist...

journal_title：Biochemistry

authors： Clore GM,Gronenborn AM,Nilges M,Ryan CA

更新日期：1987-12-01 00:00:00

abstract：:The solution of a primary 16S rRNA-binding ribosomal protein, S17, was investigated by two- and three-dimensional homonuclear and heteronuclear magnetic resonance spectroscopy. Almost complete chemical shift assignments for the 1H, 15N, and 13C resonances have been obtained. The NMR data have been rigorously analyzed ...

journal_title：Biochemistry

authors： Jaishree TN,Ramakrishnan V,White SW

更新日期：1996-03-05 00:00:00

abstract：:The pathological prion protein PrP(Sc) is the only known component of the infectious prion. In cells infected with prions, PrP(Sc) is formed posttranslationally by the refolding of the benign cell surface glycoprotein PrP(C) into an aberrant conformation. The two PrP isoforms possess very different properties, as PrP(...

journal_title：Biochemistry

authors： Tzaban S,Friedlander G,Schonberger O,Horonchik L,Yedidia Y,Shaked G,Gabizon R,Taraboulos A

更新日期：2002-10-22 00:00:00

abstract：:The hydration of CO2 catalyzed by carbonic anhydrase requires proton transfer from the zinc-bound water at the active site to solution for each cycle of catalysis. In the most efficient of the mammalian carbonic anhydrases, isozyme II, this transfer is facilitated by a proton shuttle residue, His 64. Murine carbonic a...

journal_title：Biochemistry

authors： Earnhardt JN,Qian M,Tu C,Laipis PJ,Silverman DN

更新日期：1998-05-19 00:00:00

abstract：:Kinetic and spectroscopic studies have shown that the conserved active site residue His200 of the extradiol ring-cleaving homoprotocatechuate 2,3-dioxygenase (FeHPCD) from Brevibacterium fuscum is critical for efficient catalysis. The roles played by this residue are probed here by analysis of the steady-state kinetic...

journal_title：Biochemistry

authors： Kovaleva EG,Rogers MS,Lipscomb JD

更新日期：2015-09-01 00:00:00

abstract：:Peptide nucleic acids (PNAs) are nonionic DNA/RNA mimics that can recognize complementary sequences by Watson-Crick base pairing. The neutral PNA backbone facilitates the recognition of duplex DNA by strand invasion, suggesting that antigene PNAs (agPNAs) can be important tools for exploring the structure and function...

journal_title：Biochemistry

authors： Hu J,Corey DR

更新日期：2007-06-26 00:00:00

abstract：:Stopped-flow total intensity and anisotropy experiments on single tryptophan containing mutants of yeast phosphoglycerate kinase (PGK) located in either the carboxy-terminal domain (W308 and W333), amino-terminal domain (W48 and W122), or "hinge" region (W194 and W399) were performed. The results obtained for single t...

journal_title：Biochemistry

authors： Beechem JM,Sherman MA,Mas MT

更新日期：1995-10-24 00:00:00

abstract：:Membrane vesicles isolated from Escherichia coli ML 308--225 have been analyzed by crossed immunoelectrophoresis, and immunoprecipitates corresponding to the following cellular components have been identified: ATPase (EC 3.6.1,3), two or three NADH dehydrogenases (EC 1.6.99.3), D-lactate dehydrogenase (EC 1.1.1.27), g...

journal_title：Biochemistry

authors： Owen P,Kaback HR

更新日期：1979-04-17 00:00:00

abstract：:Acholeplasma laidlawii B cells, highly enriched in saturated C14 and C15 fatty acids, have been grown at several temperatures. Conformational order in the acyl chains of live cell membranes has been monitored with Fourier-transform infrared (FT-IR) spectroscopy. Coupled CH2 wagging progressions, characteristic of the ...

journal_title：Biochemistry

authors： Moore DJ,Mendelsohn R

更新日期：1994-04-05 00:00:00

abstract：:A study of the dynamics and mechanism of the various thermotropic phase transitions undergone by the hydrated monoacylglycerides monoolein and monoelaidin, in the temperature range of 20-120 degrees C and from 0 to 5 M NaCl, has been undertaken. Measurements were made by using time-resolved X-ray diffraction at the Co...

journal_title：Biochemistry

authors： Caffrey M

更新日期：1987-10-06 00:00:00

abstract：:We report here on the Raman spectra of NADH, 3-acetylpyridine adenine dinucleotide, APAD+, and a fragment of these molecules, adenosine 5'-diphosphate ribose (ADPR) bound to the mitochondrial (mMDH) and cytoplasmic (or soluble, sMDH) forms of malate dehydrogenase. We observe changes in the Raman spectrum of the adenos...

journal_title：Biochemistry

authors： Deng H,Burgner J,Callender R

更新日期：1991-09-10 00:00:00

abstract：:Filamin A (FLNA) is a ubiquitously expressed actin cross-linking protein and a scaffold of numerous binding partners to regulate cell proliferation, migration, and survival. FLNA is a homodimer, and each subunit has an N-terminal actin-binding domain followed by 24 immunoglobulin-like repeats (R). FLNA mediates mechan...

journal_title：Biochemistry

authors： Wang L,Nakamura F

更新日期：2019-11-26 00:00:00

abstract：:The ubiquitin signaling pathway consists of hundreds of enzymes that are tightly regulated for the maintenance of cell homeostasis. Parkin is an E3 ubiquitin ligase responsible for conjugating ubiquitin onto a substrate protein, which itself can be ubiquitinated. Ataxin-3 performs the opposing function as a deubiquiti...

journal_title：Biochemistry

authors： Bai JJ,Safadi SS,Mercier P,Barber KR,Shaw GS

更新日期：2013-10-22 00:00:00

abstract：:Fluorescent and photoactivatable fluorescent derivatives of tetrodotoxin (TTX) have been synthesized. N-Methylanthraniloylglycine hydrazide, anthraniloyl hydrazide, and 2-azidoanthraniloylglycine hydrazide were coupled to the carbonyl at C6 of oxidized tetrodotoxin to form stable fluorescent hydrazones. The C6 ketone ...

journal_title：Biochemistry

authors： Angelides KJ

更新日期：1981-07-07 00:00:00

abstract：:The SfiI restriction enzyme binds to DNA as a tetramer holding two usually distant DNA recognition sites together before cleavage of the four DNA strands. To elucidate structural properties of the SfiI-DNA complex, atomic force microscopy (AFM) imaging of the complexes under noncleaving conditions (Ca2+ instead of Mg2...

journal_title：Biochemistry

authors： Lushnikov AY,Potaman VN,Oussatcheva EA,Sinden RR,Lyubchenko YL

更新日期：2006-01-10 00:00:00

abstract：:Second-harmonic light scattering (SHS) permits characterization of membrane-specific molecular transport in living cells. Herein, we demonstrate the use of time-resolved SHS for quantifying chemically induced enhancements in membrane permeability. As proof of concept, we examine the enhanced permeability of the cytopl...

journal_title：Biochemistry

authors： Wilhelm MJ,Sharifian Gh M,Dai HL

更新日期：2015-07-28 00:00:00

abstract：:Phosphotriesterase, as isolated from Pseudomonas diminuta, is capable of detoxifying widely used pesticides such as paraoxon and parathion and various mammalian acetylcholinesterase inhibitors. The enzyme requires a binuclear metal center for activity. Recently, the three-dimensional structure of the apoenzyme was sol...

journal_title：Biochemistry

authors： Benning MM,Kuo JM,Raushel FM,Holden HM

更新日期：1995-06-27 00:00:00

abstract：:The newly discovered oncogenic protein gankyrin, which contains six ankyrin repeats, has been reported to be involved in the phosphorylation and degradation of the retinoblastoma gene product, Rb. Using in vitro systems, we have identified a peptide fragment of gankyrin, 176LHLACDEERN185, which is responsible for bind...

journal_title：Biochemistry

authors： Li J,Tsai MD

更新日期：2002-03-26 00:00:00

abstract：:In order to clarify the functional and structural role of intra- and intersubunit hydrogen bonds in human hemoglobin (Hb A), we prepared two artificial beta chain mutant hemoglobins by site-directed mutagenesis. The mutant Hb Phe-37 beta, in which Trp-37 beta is replaced by Phe to remove the intersubunit hydrogen bond...

journal_title：Biochemistry

authors： Ishimori K,Imai K,Miyazaki G,Kitagawa T,Wada Y,Morimoto H,Morishima I

更新日期：1992-03-31 00:00:00

abstract：:The interaction of the Escherichia coli single-stranded DNA binding protein (SSB) with oligo- and poly-nucleotides has been studied by 270-MHz 1H NMR spectroscopy and fast kinetic techniques. d(pT)8 and poly(dT) were used to study noncooperative and cooperative binding, respectively. The H6, H1', and CH3 resonances of...

journal_title：Biochemistry

authors： Römer R,Schomburg U,Krauss G,Maass G

更新日期：1984-12-04 00:00:00

abstract：:A new class of eukaryotic protein kinases that are not homologous to members of the serine/threonine/tyrosine protein kinase superfamily was recently identified [Futey, L. M., et al. (1995) J. Biol. Chem. 270, 523-529; Ryazanov, A. G., et al. (1997) Proc. Natl. Acad. Sci. U.S.A. 94, 4884-4889]. This class includes euk...

journal_title：Biochemistry

authors： Pavur KS,Petrov AN,Ryazanov AG

更新日期：2000-10-10 00:00:00

abstract：:Light-driven outward sodium-pumping rhodopsin (NaR) was recently found in marine bacteria. Krokinobacter eikastus rhodopsin 2 (KR2) actively transports sodium and lithium ions in NaCl and LiCl, respectively, while it pumps protons in KCl. NaR has a conserved NDQ (N112, D116, and Q123 in KR2) motif, and previous studie...

journal_title：Biochemistry

authors： Abe-Yoshizumi R,Inoue K,Kato HE,Nureki O,Kandori H

更新日期：2016-10-18 00:00:00

abstract：:The specific deamidation of asparagine-71 of triosephosphate isomerase increases upon substrate binding and catalysis. This deamidation at the dimer interface initiates subunit dissociation, unfolding, and protein degradation. The apparent connection between catalysis and terminal marking supports the concept of "mole...

journal_title：Biochemistry

authors： Garza-Ramos G,Tuena de Gomez-Puyou M,Gomez-Puyou A,Yüksel KU,Gracy RW

更新日期：1994-06-07 00:00:00

abstract：:Angiotensin converting enzyme (ACE) plays a critical role in the circulating or endocrine renin-angiotensin system (RAS) as well as the local regulation that exists in tissues such as the myocardium and skeletal muscle. Here we report the high-resolution crystal structures of testis ACE (tACE) in complex with the firs...

journal_title：Biochemistry

authors： Natesh R,Schwager SL,Evans HR,Sturrock ED,Acharya KR

更新日期：2004-07-13 00:00:00

abstract：:A double mutant cycle has been used to evaluate interaction energies between the global stabilizer mutation asparagine 52 --> isoleucine (N52I) in iso-1-cytochrome c and mutations producing single surface histidines at positions 26, 33, 39, 54, 73, 89, and 100. These histidine mutation sites are distributed through th...

journal_title：Biochemistry

authors： Wandschneider E,Hammack BN,Bowler BE

更新日期：2003-09-16 00:00:00

abstract：:Most proteins are denatured by heat treatment, and the process is usually irreversible. However, some proteins, such as hyperthermophilic proteins are known to be stable even at the boiling temperature of water. We here describe a systematic investigation of thermal behavior of proteins by purifying and characterizing...

journal_title：Biochemistry

authors： Kim TD,Ryu HJ,Cho HI,Yang CH,Kim J

更新日期：2000-12-05 00:00:00

abstract：:A short chain dehydrogenase like l-threonine 3-dehydrogenase (SDR-TDH) from metagenome data (mtTDH) was identified by database mining. Its enzymatic properties suggested that mtTDH has unique characteristics relative to other SDR-TDHs, including two mesophilic and thermophilic SDR-TDHs identified in this study. The ac...

journal_title：Biochemistry

authors： Motoyama T,Nakano S,Yamamoto Y,Tokiwa H,Asano Y,Ito S

更新日期：2017-10-31 00:00:00

abstract：:The phosphatase activity of the homodimeric NRII protein of Escherichia coli is activated by the PII protein and requires all three domains of NRII. Mutations in the N-terminal domain (L16R), central domain (A129T), C-terminal domain PII-binding site (S227R), and C-terminal domain ATP-lid (Y302N) of NRII result in dim...

journal_title：Biochemistry

authors： Pioszak AA,Ninfa AJ

更新日期：2003-07-29 00:00:00