Abstract：
:Second-harmonic light scattering (SHS) permits characterization of membrane-specific molecular transport in living cells. Herein, we demonstrate the use of time-resolved SHS for quantifying chemically induced enhancements in membrane permeability. As proof of concept, we examine the enhanced permeability of the cytoplasmic membrane in living Escherichia coli following addition of extracellular adenosine triphosphate (ATPe). The transport rate of the hydrophobic cation, malachite green, increases nearly an order of magnitude following addition of 0.1 mM ATPe. The absence of an ATPe-enhanced permeability in liposomes strongly suggests the induced effect is protein-mediated. The utility of SHS for elucidating the mechanism of action of antimicrobials is discussed.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Wilhelm MJ,Sharifian Gh M,Dai HLdoi
10.1021/acs.biochem.5b00600subject
Has Abstractpub_date
2015-07-28 00:00:00pages
4427-30issue
29eissn
0006-2960issn
1520-4995journal_volume
54pub_type
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