Escherichia coli single-stranded DNA binding protein is mobile on DNA: 1H NMR study of its interaction with oligo- and polynucleotides.

abstract：:Atrazine chlorohydrolase (AtzA) from Pseudomonas sp. ADP initiates the metabolism of the herbicide atrazine by catalyzing a hydrolytic dechlorination reaction to produce hydroxyatrazine. Sequence analysis revealed AtzA to be homologous to metalloenzymes within the amidohydrolase protein superfamily. AtzA activity was ...

journal_title：Biochemistry

authors： Seffernick JL,McTavish H,Osborne JP,de Souza ML,Sadowsky MJ,Wackett LP

更新日期：2002-12-03 00:00:00

abstract：:Human fibroblast interferon binds to a concanavalin A-agarose (Con A-Sepharose) equilibrated with methyl alpha-D-mannopyranoside, or levan; in contrast, it is only partially retarded on a similar column equilibrated with ethylene glycol. Interferon does not bind, however, to a lectin column equilibrated with both meth...

journal_title：Biochemistry

authors： Davey MW,Sulkowski E,Carter WA

更新日期：1976-02-10 00:00:00

abstract：:In order to elucidate the molecular mechanisms responsible for the apparent nonlinear behavior of the temperature dependence of the redox potential of Hydrogenobacter thermophilus cytochrome c552 [Takahashi, Y., Sasaki, H., Takayama, S. J., Mikami, S., Kawano, S., Mita, H., Sambongi, Y., and Yamamoto, Y. (2006) Bioche...

journal_title：Biochemistry

authors： Takayama SJ,Takahashi YT,Mikami S,Irie K,Kawano S,Yamamoto Y,Hemmi H,Kitahara R,Yokoyama S,Akasaka K

更新日期：2007-08-14 00:00:00

abstract：:Oligonucleotides labeled with hexachlorofluorescein (hex) have enabled the interaction of the restriction endonuclease EcoRV with DNA to be evaluated using fluorescence anisotropy. The sensitivity of hex allowed measurements at oligonucleotide concentrations as low as 1 nM, enabling K(D) values in the low nanomolar ra...

journal_title：Biochemistry

authors： Reid SL,Parry D,Liu HH,Connolly BA

更新日期：2001-02-27 00:00:00

abstract：:Myelin basic protein (MBP) is a multifunctional protein involved in maintaining the stability and integrity of the myelin sheath by a variety of interactions with membranes and with cytoskeletal and other proteins. A central segment of MBP is highly conserved in mammals and consists of a membrane surface-associated am...

journal_title：Biochemistry

authors： Polverini E,Rangaraj G,Libich DS,Boggs JM,Harauz G

更新日期：2008-01-08 00:00:00

abstract：:D-Xylose isomerase (XI) is a heat-stable homotetrameric enzyme used in industry for the production of high-fructose corn syrups by isomerization of D-glucose into D-fructose. To carry out biochemical and structural studies of this enzyme and of its engineered variants, a rapid and convenient method of purification of ...

journal_title：Biochemistry

authors： Mrabet NT

更新日期：1992-03-17 00:00:00

abstract：:The [2Fe-2S] cluster of the Rieske iron-sulfur protein is held between two loops of the protein that are connected by a disulfide bridge. We have replaced the two cysteines that form the disulfide bridge in the Rieske protein of Saccharomyces cerevisiae with tyrosine and leucine, and tyrosine and valine, to evaluate t...

journal_title：Biochemistry

authors： Merbitz-Zahradnik T,Zwicker K,Nett JH,Link TA,Trumpower BL

更新日期：2003-11-25 00:00:00

abstract：:Chemical-modification studies on submitochondrial particle pyridine dinucleotide transhydrogenase (EC 1.6.1.1) demonstrate the presence of one class of sulfhydryl group in the nicotinamide adenine dinucleotide phosphate (NADP) site and another peripheral to the active site. Reaction of the peripheral sulfhydryl group ...

journal_title：Biochemistry

authors： Earle SR,O'Neal SG,Fisher RR

更新日期：1978-10-31 00:00:00

abstract：:Human fibrinogen gamma-module comprising residues gamma148-411 was expressed in Escherichia coli and refolded in vitro. Differential scanning calorimetry revealed that in addition to the two previously identified independently folded thermolabile domains, one in each half of the module, the gamma-module also contains ...

journal_title：Biochemistry

authors： Medved L,Litvinovich S,Ugarova T,Matsuka Y,Ingham K

更新日期：1997-04-15 00:00:00

abstract：:Properties of the interaction of bleomycin (Blm) and metallobleomycins [M = Zn, Cu(II), Fe(III), and HO(2)-Co(III)] with site-specific and nonspecific DNA oligomers, d(GGAAGCTTCC)(2) (I) and d(GGAAATTTCC)(2) (II), respectively, were investigated. With both 10-mers association constants increased in the series Blm A(2)...

journal_title：Biochemistry

authors： Li W,Zhao C,Xia C,Antholine WE,Petering DH

更新日期：2001-06-26 00:00:00

abstract：:Equilibrium binding isotherms have been measured as a function of temperature for the interaction of 2- and 8-aminoadenosine with poly(U) by continuous, constant-volume ultrafiltration. An analytical method is described for determining the entire isotherm in a single experiment on a preequilibrated ligand--polymer sol...

journal_title：Biochemistry

authors： Roy KB,Miles HT

更新日期：1982-01-05 00:00:00

abstract：:PDZ domains are among the most abundant protein modules in the known genomes. Their main function is to provide scaffolds for membrane-associated protein complexes by binding to the cytosolic, C-terminal fragments of receptors, channels, and other integral membrane proteins. Here, using both heteronuclear NMR and sing...

journal_title：Biochemistry

authors： Grembecka J,Cierpicki T,Devedjiev Y,Derewenda U,Kang BS,Bushweller JH,Derewenda ZS

更新日期：2006-03-21 00:00:00

abstract：:Chloramphenicol acetyltransferase (CAT) catalyzes the acetyl-CoA-dependent acetylation of chloramphenicol by a ternary complex mechanism with a rapid equilibrium and essentially random order of addition of substrates. Such a kinetic mechanism for a two-substrate reaction provides an opportunity to compare the affinity...

journal_title：Biochemistry

authors： Ellis J,Bagshaw CR,Shaw WV

更新日期：1991-11-05 00:00:00

abstract：:In the minor fraction of HDL3 containing alpha-tocopherol (alphaTocOH), selective one-electron oxidation of Trp and Tyr residues of apolipoproteins A-I and A-II by *Br2- radical-anions produces the corresponding semioxidized species, TyrO* and *Trp. Repair of TyrO* by endogenous alphaTocOH generates the alpha-tocopher...

journal_title：Biochemistry

authors： Filipe P,Patterson LK,Bartels DM,Hug GL,Freitas JP,Mazière JC,Santus R,Morlière P

更新日期：2007-12-11 00:00:00

abstract：:Protein kinases are key regulators of diverse signaling networks critical for growth and development. Protein kinase A (PKA) is an important kinase prototype that phosphorylates protein targets at Ser and Thr residues by converting ATP to ADP. Mg(2+) ions play a crucial role in regulating phosphoryl transfer and can l...

journal_title：Biochemistry

authors： Khavrutskii IV,Grant B,Taylor SS,McCammon JA

更新日期：2009-12-08 00:00:00

abstract：:Misaligned structures can result from strand slippage during DNA replication and, if not repaired, would lead to mutations. Previously, we showed that strand slippage can occur upon misincorporation of a dNTP opposite thymine and cytosine templates, resulting in a misaligned structure with a T- or C-bulge. The formati...

journal_title：Biochemistry

authors： Chi LM,Lam SL

更新日期：2009-12-08 00:00:00

abstract：:Peptides prepared from the amino termini of pro alpha 1(I) and pro alpha 1(III) collagen chains inhibit the production of pro alpha 1(I) and pro alpha 2 by rat calvaria rna in a reticulocyte cell-free system. The synthesis of other proteins was not altered, suggesting a specific effect on collagen production. Various ...

journal_title：Biochemistry

authors： Paglia L,Wilczek J,de Leon LD,Martin GR,Hörlein D,Müller P

更新日期：1979-10-30 00:00:00

abstract：:Resonance energy-transfer approaches have been used to directly monitor the interactions of the GTP gamma S-bound alpha subunit of transducin (alpha T GTP gamma S) with the retinal cyclic GMP phosphodiesterase (PDE). The PDE was labeled with 5-(iodoacetamido) fluorescein (IAF-PDE) and served as the fluorescence donor ...

journal_title：Biochemistry

authors： Erickson JW,Cerione RA

更新日期：1991-07-23 00:00:00

abstract：:We have investigated the action of the human DNA polymerase ε (hpol ε) and η (hpol η) catalytic cores on G-quadruplex (G4) DNA substrates derived from the promoter of the c-MYC proto-oncogene. The translesion enzyme hpol η exhibits a 6.2-fold preference for binding to G4 DNA over non-G4 DNA, while hpol ε binds both G4...

journal_title：Biochemistry

authors： Eddy S,Maddukuri L,Ketkar A,Zafar MK,Henninger EE,Pursell ZF,Eoff RL

更新日期：2015-05-26 00:00:00

abstract：:Cerebrovascular deposition of fibrillar 39-42 amino acid amyloid beta-protein (Abeta), a condition known as cerebral amyloid angiopathy (CAA), is a key pathological feature of Alzheimer's disease and related disorders including hereditary cerebral hemorrhage with amyloidosis-Dutch type (HCHWA-D). Severe cases of CAA, ...

journal_title：Biochemistry

authors： Wagner MR,Keane DM,Melchor JP,Auspaker KR,Van Nostrand WE

更新日期：2000-06-27 00:00:00

abstract：:We have obtained the 470 MHz 19F NMR spectra of wild type [4-F]Trp-labeled myoglobins (MbCO, MbO2, deoxyMb, metMb, and MbCN) and hemoglobins (HbCO, HbO2, and deoxyHb), as well as those of several mutants (W7F Mb, betaW15F Hb, betaW37S Hb, and betaY130F Hb, all as the carbonmonoxy adducts), prepared via site-directed m...

journal_title：Biochemistry

authors： Pearson JG,Montez B,Le H,Oldfield E,Chien EY,Sligar SG

更新日期：1997-03-25 00:00:00

abstract：:The generation of multiprotein complexes at receptors and adapter proteins is crucial for the activation of intracellular signaling pathways. In this study, we used multiple biochemical and biophysical methods to examine the binding properties of several SH2 and SH3 domain-containing signaling proteins as they interac...

journal_title：Biochemistry

authors： Houtman JC,Higashimoto Y,Dimasi N,Cho S,Yamaguchi H,Bowden B,Regan C,Malchiodi EL,Mariuzza R,Schuck P,Appella E,Samelson LE

更新日期：2004-04-13 00:00:00

abstract：:The structure/function properties of functional groups in the leadzyme have been studied by assaying the activity of analog ribozymes generated by the systematic substitution of modified nucleotides in the internal loop region of the ribozyme. Guanosine analogs introduced at positions 4 and 7 occupied by guanosine in ...

journal_title：Biochemistry

authors： Chartrand P,Usman N,Cedergren R

更新日期：1997-03-18 00:00:00

abstract：:We have examined the DNA damage produced by reaction of peroxyl radicals with human fibroblast DNA. DNA damage consisted of both strand breaks and base modifications. The extent of strand breaks and base modifications induced as a function of peroxyl radical concentration was determined by quantitation of fragment siz...

journal_title：Biochemistry

authors： Rodriguez H,Valentine MR,Holmquist GP,Akman SA,Termini J

更新日期：1999-12-14 00:00:00

abstract：:Glycophorin A is a sialoglycoprotein isolated from human erythrocyte membranes which seems to exist as stable dimeric complexes in the presence of sodium dodecyl sulfate. When analyzed by dodecyl sulfate acrylamide electrophoresis this molecule forms two PAS-stainable bands (PAS-U and PAS-2) which are reversibly inter...

journal_title：Biochemistry

authors： Furthmayr H,Marchesi VT

更新日期：1976-03-09 00:00:00

abstract：:Cholesterol (Chol) in phosphatidylcholine large unilamellar vesicles (PC LUV) modulated interaction of the bilayers with a class A amphipathic peptide, Ac-18A-NH2: Chol increased the peptide binding capacity and reduced the affinity together with the peptide-induced leakage of calcein from LUV. Similar effects of Chol...

journal_title：Biochemistry

authors： Egashira M,Gorbenko G,Tanaka M,Saito H,Molotkovsky J,Nakano M,Handa T

更新日期：2002-03-26 00:00:00

abstract：:Antifreeze proteins (AFPs) inhibit the growth of ice by binding to the surface of ice crystals, preventing the addition of water molecules to cause a local depression of the freezing point. AFPs from insects are much more effective at depressing the freezing point than fish AFPs. Here, we have investigated the possibi...

journal_title：Biochemistry

authors： Marshall CB,Tomczak MM,Gauthier SY,Kuiper MJ,Lankin C,Walker VK,Davies PL

更新日期：2004-01-13 00:00:00

abstract：:The binding of rabbit globin mRNA to the 25-kDa cap binding protein eIF-4E from human erythrocytes was found to be 5.3-fold stronger than the binding of the cap analogue m7GpppG to eIF-4E [Gross et al. (1990) Biochemistry 29, 5008-5012]. In order to investigate whether this effect is due to the longer sequence of nucl...

journal_title：Biochemistry

authors： Carberry SE,Friedland DE,Rhoads RE,Goss DJ

更新日期：1992-02-11 00:00:00

abstract：:Heparan sulfate (HS) proteoglycans (PGs) interact with a number of extracellular signaling proteins, thereby playing an essential role in the regulation of many physiological processes. These interactions are important for both normal signal transduction and regulation of the tissue distribution of signaling molecules...

journal_title：Biochemistry

authors： Zhang F,McLellan JS,Ayala AM,Leahy DJ,Linhardt RJ

更新日期：2007-04-03 00:00:00

abstract：:L-Aspartate oxidase (Laspo) catalyzes the conversion of L-Asp to iminoaspartate, the first step in the de novo biosynthesis of NAD(+). This bacterial pathway represents a potential drug target since it is absent in mammals. The Laspo R386L mutant was crystallized in the FAD-bound catalytically competent form and its t...

journal_title：Biochemistry

authors： Bossi RT,Negri A,Tedeschi G,Mattevi A

更新日期：2002-03-05 00:00:00