Abstract:
:The toxin RelE is a ribosome-dependent endoribonuclease implicated in diverse cellular processes, including persistence. During amino acid starvation, RelE inhibits translation by cleaving ribosomal A-site mRNA. Although RelE is structurally similar to other microbial endoribonucleases, the active-site amino acid composition differs substantially and lacks obvious candidates for general acid-base functionality. Highly conserved RelE residues (Lys52, Lys54, Arg61, Arg81, and Tyr87) surround the mRNA scissile phosphate, and specific 16S rRNA contacts further contribute to substrate positioning. We used a single-turnover kinetic assay to evaluate the catalytic importance of individual residues in the RelE active site. Within the context of the ribosome, RelE rapidly cleaves A-site mRNA at a rate similar to those of traditional ribonucleases. Single-turnover rate constants decreased between 10(2)- and 10(6)-fold for the RelE active-site mutants of Lys52, Lys54, Arg61, and Arg81. RelE may principally promote catalysis via transition-state charge stabilization and leaving-group protonation, in addition to achieving in-line substrate positioning in cooperation with the ribosome. This kinetic analysis complements structural information to provide a foundation for understanding the molecular mechanism of this atypical endoribonuclease.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Griffin MA,Davis JH,Strobel SAdoi
10.1021/bi401325csubject
Has Abstractpub_date
2013-12-03 00:00:00pages
8633-42issue
48eissn
0006-2960issn
1520-4995journal_volume
52pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Fibronectin (FN) is an extracellular matrix (ECM) protein found soluble in corporal fluids or as an insoluble fibrillar component incorporated in the ECM. This phenomenon implicates structural changes that expose FN binding sites and activate the protein to promote intermolecular interactions with other FN. We have in...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0347104
更新日期:2004-02-17 00:00:00
abstract::We have previously shown that both recombinant apo(a) and native Lp(a) inhibit the binding of Glu-plasminogen to fibrin surfaces [Fleury & Anglés-Cano (1991) Biochemistry 30, 7630-7638; Rouy et al. (1992) Biochemistry 31, 6332-6339]. The aim of the present study was to characterize the mechanism of this inhibition and...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00041a011
更新日期:1995-10-17 00:00:00
abstract::We describe a fluorescence assay that can be used to monitor helicase-catalyzed unwinding of duplex DNA continuously in real time. The assay is based on the observation that fluorescence resonance energy transfer (FRET) occurs between donor (fluorescein) and acceptor (hexachlorofluorescein) fluorophores that are in cl...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00251a044
更新日期:1994-11-29 00:00:00
abstract::The "stalk" of the prokaryotic 50S ribosomal subunit is comprised of four copies of the protein L7/L12. In Escherichia coli, L7/L12 is a dimeric protein at micromolar concentrations, which is able to undergo rapid subunit exchange. A recent structural study indicated a tetrameric arrangement of the L12 proteins isolat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi048015n
更新日期:2005-03-08 00:00:00
abstract::Intrinsic factor (IF(50)) is a cobalamin (Cbl)-transporting protein of 50 kDa, which can be cleaved into two fragments: the 30 kDa N-terminal peptide IF(30) and the 20 kDa C-terminal glycopeptide IF(20). Experiments on binding of Cbl to IF(30), IF(20), and IF(50) revealed comparable association rate constants (k(+)(Cb...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi047936v
更新日期:2005-03-08 00:00:00
abstract::Prokaryotic and eukaryotic cytochrome c oxidases and several bacterial ubiquinol oxidases compose a superfamily of heme-copper oxidases. These enzymes are terminal components of aerobic respiratory chains, the principal energy-generating systems of aerobic organisms. Two such heme-copper oxidases are the aa3-type cyto...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00091a046
更新日期:1993-10-12 00:00:00
abstract::To be fully active and participate in the metabolism of phosphorylated nucleotides, most nucleoside diphosphate kinases (NDPKs) have to assemble into stable hexamers. Here we studied the role played by six intersubunit salt bridges R80-D93 in the stability of NDPK from the pathogen Mycobacterium tuberculosis ( Mt). Mu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b01308
更新日期:2019-03-12 00:00:00
abstract::Lipocortin I is a 39-kilodalton membrane-associated protein that in A431 cells is phosphorylated on tyrosine in response to epidermal growth factor (EGF). We have used recombinant human lipocortin I as a substrate for several protein kinases and identified phosphorylated residues by a combination of peptide mapping an...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00410a024
更新日期:1988-05-17 00:00:00
abstract::Glutaraldehyde treatment of electroplax membrane preparations of Na,K-ATPase leads to irreversible changes in the enzymic behavior of the protein, which are not due to modification of the active site. When the glutaraldehyde treatment is carried out in a medium containing K+ and without Na+, the "K+-modified enzyme" s...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00288a016
更新日期:1983-09-13 00:00:00
abstract::The electronic (ultraviolet) circular dichroism (UVCD) and vibrational circular dichroism (VCD) of 20 proteins are systematically compared as to their relationship to the secondary structures of these proteins. The UVCD spectra are statistically treated by use of the same factor analysis methods used previously for VC...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00242a012
更新日期:1991-07-16 00:00:00
abstract::Epigallocatechin-3-gallate (EGCG) is a catechin found in green tea that can inhibit the amyloid formation of a wide variety of proteins. EGCG's ability to prevent or redirect the amyloid formation of so many proteins may reflect a common mechanism of action, and thus, greater molecular-level insight into how it exerts...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.0c00043
更新日期:2020-03-17 00:00:00
abstract::To characterize the roles played by Glu44 and Glu56 of cytochrome b5 in the formation of the electrostatic complex between cytochrome c and cytochrome b5, the Glu44, Glu56, or both sites were changed to alanine by site-directed mutagenesis. The influence of these two residues on the protein stability was probed by inv...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9805036
更新日期:1998-10-06 00:00:00
abstract::Phosphotriesterase, as isolated from Pseudomonas diminuta, is capable of detoxifying widely used pesticides such as paraoxon and parathion and various mammalian acetylcholinesterase inhibitors. The enzyme requires a binuclear metal center for activity. Recently, the three-dimensional structure of the apoenzyme was sol...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00025a002
更新日期:1995-06-27 00:00:00
abstract::Specificity of the binding of sterols and related compounds with purified F-protein (fusion protein) of the HVJ (Sendai virus) was studied by binding competition with [3H]cholesterol. Requirement for cholesterol or the A/B ring trans structure and nonrequirement for the 3-hydroxyl group were found in this binding. Bin...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00404a035
更新日期:1988-02-23 00:00:00
abstract::Tandem DNA lesions containing two contiguously damaged nucleotides are commonly formed by ionizing radiation. Their effects on replication in mammalian cells are largely unknown. Replication of isolated 2-deoxyribonolactone (L), thymine glycol (Tg), and tandem lesion 5'-LTg was examined in human cells. Although nearly...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.9b01058
更新日期:2020-02-04 00:00:00
abstract::The accessibility of the asparagine-linked carbohydrate chains of human thyrotropin (hTSH) and free alpha and beta subunits was investigated by their susceptibility to endoglycosidases H and F as well as to peptide:N-glycosidase F. Iodinated hTSH or subunits were incubated with a commercial enzyme preparation containi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00392a040
更新日期:1987-09-08 00:00:00
abstract::The structure and function of iron(II)-ruthenium(II) hybrid hemoglobins alpha(Ru-CO)2 beta(Fe)2 and alpha(Fe)2 beta(Ru-CO)2, which can serve as models for the intermediate species of the oxygenation step in native human adult hemoglobin, were investigated by measuring oxygen equilibrium curves and the Fe(II)-N epsilon...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00447a050
更新日期:1989-10-17 00:00:00
abstract::To elucidate a unique mechanism for the quinol oxidation in the Escherichia coli cytochrome bo, we applied pulse radiolysis technique to the wild-type enzyme with or without a single bound ubiquinone-8 at the high-affinity quinone binding site (Q(H)), using N-methylnicotinamide (NMA) as an electron mediator. With the ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0014094
更新日期:2000-12-19 00:00:00
abstract::The crystal structure of Escherichia coli GAR Tfase at 2.1 A resolution in complex with 10-formyl-5,8,10-trideazafolic acid (10-formyl-TDAF, K(i) = 260 nM), an inhibitor designed to form an enzyme-assembled multisubstrate adduct with the substrate, beta-GAR, was studied to determine the exact nature of its inhibitory ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi991888a
更新日期:1999-12-21 00:00:00
abstract::Murine double-minute clone 2 protein (MDM2) is an E3 ubiquitin ligase that regulates the turnover of several cellular factors including the p53 tumor suppressor protein. As part of the mechanism of p53 induction in response to DNA damage, a cluster of serine residues within the central acidic domain of MDM2 become hyp...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0489255
更新日期:2004-12-28 00:00:00
abstract::The protein phosphatase 2C (PP2C) family represents one of the four major protein Ser/Thr phosphatase activities in mammalian cells and contains at least 13 distinct gene products. Although PP2C family members regulate a variety of cellular functions, mechanisms of regulation of their activities are largely unknown. H...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi800067p
更新日期:2008-07-08 00:00:00
abstract::The structures at 2.0 and 2.25 A resolution of native and recombinant nitrite reductase from Alcaligenes faecalis show that they are identical to each other and very similar to nitrite reductase from Achromobacter cycloclastes. The crystallographic structure of a mutant, M150E, which unlike the wild-type protein canno...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00038a003
更新日期:1995-09-26 00:00:00
abstract::The ionization and phase behavior of 1,2-dipalmitoyl-sn-glycero-3-phosphoserine have been investigated under a variety of condtions by several different methods. As measured by turbidity changes, the temperature of the crystal-liquid crystal phase transition of this lipid is influenced by pH and mono- and divalent ca...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00649a025
更新日期:1976-02-24 00:00:00
abstract::Previous work has described the novel ability to modulate in vitro the activity of restriction endonuclease NaeI from Nocardia aerocoligenes by using cleavable DNA and spermidine [Conrad & Topal (1989) Proc. Natl. Acad. Sci. U.S.A. 86, 9707-9711]. In this paper we report the results of a study of 49 type II restrictio...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00223a035
更新日期:1991-03-05 00:00:00
abstract::Escherichia coli DNA photolyase contains 1,5-dihydro-FAD (FADH2) plus 5,10-methenyl-tetrahydrofolate (5,10-CH+-H4folate). Both chromophores are fluorescent, and either can function as a sensitizer in catalysis. At 77 K separate fluorescence emission bands are observed for FADH2 (lambda max = 505 nm, shoulder at 540 nm...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00425a007
更新日期:1988-12-13 00:00:00
abstract::The role of the peripheral reaction center chlorophyll a molecule associated with His117 of the D2 polypeptide in photosystem II was investigated in Synechocystis sp. PCC 6803 using a combination of steady state, pump-probe, and picosecond time-resolved fluorescence spectroscopy. Data were obtained from intact cells a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi000476v
更新日期:2000-11-21 00:00:00
abstract::The conformation of the trans-anti-(1S,2R,3S,4R)-N(2)-[1-(1,2,3,4-tetrahydro-2,3,4-trihydroxybenz[a]anthracenyl)]-2'-deoxyguanosyl adduct in d(G(1)G(2)C(3)A(4)G(5)X(6)T(7)G(8)G(9)T(10)G(11)).d(C(12)A(13)C(14)C(15)A(16)C(17)C(18)T(19)G(20)C(21)C(22)), bearing codon 12 of the human N-ras protooncogene (underlined), was ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi020462k
更新日期:2003-03-04 00:00:00
abstract::Lamin A protein, encoded by the LMNA gene, belongs to the type V intermediate filament protein family and is a major nuclear protein component of higher metazoan organisms, including humans. Lamin A along with B-type lamins impart structural rigidity to the nucleus by forming a lamina that is closely apposed to the in...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi400337t
更新日期:2013-06-18 00:00:00
abstract::The aminolysis of the depsipeptide m-[[(phenylacetyl)glycyl]oxy]benzoic acid (1) by D-phenylalanine, catalyzed by the beta-lactamase of Enterobacter cloacae P99, is inhibited by the product of the reaction, (phenylacetyl)glycyl-D-phenylalanine (2), by the peptide analogue of 1, m-[(phenylacetyl)-glycinamido]benzoic ac...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00443a015
更新日期:1989-08-22 00:00:00
abstract::The metal-dependent deacetylase LpxC catalyzes the first committed step of lipid A biosynthesis in Gram-negative bacteria. Accordingly, LpxC is an attractive target for the development of inhibitors that may serve as potential new antibiotics for the treatment of Gram-negative bacterial infections. Here, we report the...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi060823m
更新日期:2006-07-04 00:00:00