Prb1 Protease Activity Is Required for Its Recognition by the F-Box Protein Saf1.

abstract：:Various 6-alkylubiquinone or 6-(omega-haloalkyl)ubiquinone derivatives were synthesized through a radical coupling reaction between alkanoyl or omega-haloalkanoyl peroxides and ubiquinone 0. The latter was synthesized from 2-methoxy-4-methylphenol via nitration, methylation, reduction, and oxidation by modifications o...

journal_title：Biochemistry

authors： Yu CA,Yu L

更新日期：1982-08-17 00:00:00

abstract：:Malate dehydrogenase occurs in virtually all eucaryotic cells in mitochondrial and cytoplasmic forms, both of which are composed of two identical subunits. The reactivation of the mitochondrial isoenzyme has been the subject of previous studies [Jaenicke, R., Rudolph, R., & Heider, I. (1979) Biochemistry 18, 1217-1223...

journal_title：Biochemistry

authors： Rudolph R,Fuchs I,Jaenicke R

更新日期：1986-04-08 00:00:00

abstract：:The periplasmic protein CusF, as a part of the CusCFBA efflux complex, plays a role in resistance to elevated levels of copper and silver in Escherichia coli. Although homologues have been identified in other Gram-negative bacteria, the substrate of CusF and its precise role in metal resistance have not been described...

journal_title：Biochemistry

authors： Kittleson JT,Loftin IR,Hausrath AC,Engelhardt KP,Rensing C,McEvoy MM

更新日期：2006-09-19 00:00:00

abstract：:Mycobacterium tuberculosis and many other members of the Actinomycetes family produce mycothiol, i.e., 1-d-myo-inosityl-2-(N-acetyl-l-cysteinyl)amido-2-deoxy-alpha-d-glucopyranoside (MSH or AcCys-GlcN-Ins), to act against oxidative and antibiotic stress. The biosynthesis of MSH is essential for cell growth and has bee...

journal_title：Biochemistry

authors： Fan F,Luxenburger A,Painter GF,Blanchard JS

更新日期：2007-10-09 00:00:00

abstract：:A series of human lymphoblastoid cell lines derived from nonleukemic donors are known to be cysteine prototrophs (cys+), while several lymphoblastoid lines derived from leukemic donors are cysteine auxotrophs (cys-). We have tested representative cell lines of each type for their content of cystathionase enzyme activi...

journal_title：Biochemistry

authors： Glode LM,Kriegler MP,Livingston DM

更新日期：1981-03-03 00:00:00

abstract：:Interaction of the peptide antibiotic alamethicin with phospholipid vesicles has been monitored by changes in its circular dichroic and fluorescent properties. The data are consistent with an incorporation of the peptide in the lipid bilayer. Aggregation of alamethicin in the membrane phase is evident from a character...

journal_title：Biochemistry

authors： Rizzo V,Stankowski S,Schwarz G

更新日期：1987-05-19 00:00:00

abstract：:The oxycomplexes (compound III, oxyperoxidase) of two lignin peroxidase isozymes, H1 (pI = 4.7) and H8 (pI = 3.5), were characterized in the present study. After generation of the ferroperoxidase by photochemical reduction with deazoflavin in the presence of EDTA, the oxycomplex is formed by mixing ferroperoxidase wit...

journal_title：Biochemistry

authors： Cai DY,Tien M

更新日期：1990-02-27 00:00:00

abstract：:The two disparate functions of DCoH1 (dimerization cofactor of HNF-1)/PCD (pterin-4a-carbinolamine dehydratase) are associated with a change in oligomeric state. DCoH dimers enhance the activity of the diabetes-associated transcription factor HNF-1α (hepatocyte nuclear factor-1α), while the PCD activity of DCoH1 homot...

journal_title：Biochemistry

authors： Rho H,Jones CN,Rose RB

更新日期：2010-11-30 00:00:00

abstract：:We determined the effect of vertebrate albumins on the stability of several physiologically relevant prostaglandins. All naturally occurring prostaglandins with beta-hydroxy ketone group decomposed by first-order kinetics, dependent on the albumin concentration in 0.1 M, pH 7.4, buffer at 37 degrees C. Even subphysiol...

journal_title：Biochemistry

authors： Fitzpatrick FA,Waynalda MA

更新日期：1981-10-13 00:00:00

abstract：:The translocation (T) domain of diphtheria toxin plays a critical role in moving the catalytic domain across the endosomal membrane. Translocation/insertion is triggered by a decrease in pH in the endosome where conformational changes of T domain occur through several kinetic intermediates to yield a final trans-membr...

journal_title：Biochemistry

authors： Li J,Rodnin MV,Ladokhin AS,Gross ML

更新日期：2014-11-04 00:00:00

abstract：:Amyloid diseases make up a set of fatal disorders in which proteins aggregate to form fibrils that deposit in tissues throughout the body. Amyloid-associated diseases are challenging to study because amyloid formation occurs on time scales that span several orders of magnitude and involve heterogeneous, interconvertin...

journal_title：Biochemistry

authors： Childers MC,Daggett V

更新日期：2019-11-05 00:00:00

abstract：:DNA damage may alter the outcome of protein-nucleic acid interactions. The malondialdehyde-deoxyguanosine adduct, 3-(2'-deoxy-beta-d-erythro-pentofuranosyl)pyrimido[1,2-alpha]purin-10-(3H)-one (M(1)dG), miscodes in vivo and in vitro. M(1)dG is an exocyclic adduct that undergoes ring-opening in duplex DNA to form the a...

journal_title：Biochemistry

authors： VanderVeen LA,Druckova A,Riggins JN,Sorrells JL,Guengerich FP,Marnett LJ

更新日期：2005-04-05 00:00:00

abstract：:Translocator protein 18 kDa (TSPO) was previously known as the peripheral benzodiazepine receptor (PBR) in eukaryotes, where it is mainly localized to the mitochondrial outer membrane. Considerable evidence indicates that it plays regulatory roles in steroidogenesis and apoptosis and is involved in various human disea...

journal_title：Biochemistry

authors： Li F,Liu J,Liu N,Kuhn LA,Garavito RM,Ferguson-Miller S

更新日期：2016-05-24 00:00:00

abstract：:The Wiskott-Aldrich syndrome protein (WASP) and neural WASP (N-WASP) are key players in regulating actin cytoskeleton via the Arp2/3 complex. It has been widely reported that the WASP proteins are activated by Rho family small GTPase Cdc42 and that Rac1 acts through SCAR/WAVE proteins. However, a systematic study of t...

journal_title：Biochemistry

authors： Tomasevic N,Jia Z,Russell A,Fujii T,Hartman JJ,Clancy S,Wang M,Beraud C,Wood KW,Sakowicz R

更新日期：2007-03-20 00:00:00

abstract：:The initial steps of actomyosin subfragment 1 (acto-S1) ATPase (dissociation and binding of ATP) were studied at -15 degrees C with 40% ethylene glycol as antifreeze. The dissociation kinetics were followed by light scattering in a stopped-flow apparatus, and the binding of ATP was followed by the ATP chase method in ...

journal_title：Biochemistry

authors： Tesi C,Travers F,Barman T

更新日期：1990-02-20 00:00:00

abstract：:To better elucidate RNA structure-function relationships and to improve the design of pharmaceutical agents that target specific RNA motifs, an understanding of RNA primary, secondary, and tertiary structure is necessary. The prediction of RNA secondary structure from sequence is an intermediate step in predicting RNA...

journal_title：Biochemistry

authors： Hausmann NZ,Znosko BM

更新日期：2012-07-03 00:00:00

abstract：:Phospholipase D has been purified 680-fold from an acetone powder of savoy cabbage in an overall yield of 30%. The purification involves solubilization of the acetone powder in a Ca2+-containing buffer and subsequent ammonium sulfate fractionation. Gel filtration on Sephadex G-200 and hydrophobic affinity chromatograp...

journal_title：Biochemistry

authors： Allgyer TT,Wells MA

更新日期：1979-11-27 00:00:00

abstract：:Hybridization to RNA is important for many applications, including antisense therapeutics, RNA interference, and microarray screening. Similar thermodynamic stabilities of A-U and G-U base pairs result in difficulties in selective binding to RNA. Moreover, A-U pairs are weaker than G-C pairs so that binding is sometim...

journal_title：Biochemistry

authors： Carlucci M,Kierzek E,Olejnik A,Turner DH,Kierzek R

更新日期：2009-11-24 00:00:00

abstract：:The complete amino acid sequence of a small, basic protein with cardiotoxic activity is described. This toxin, designated Naja naja F8, was isolated from the venom of Naja naja, of Cambodian origin, by gel filtration on Sephadex G-75 followed by gradient ion exchange chromatography on Bio-Rex 70. The cardiotoxin F8, m...

journal_title：Biochemistry

authors： Fryklund L,Eaker D

更新日期：1975-07-01 00:00:00

abstract：:Aflatoxin B1 (AFB1) exo-8,9-epoxide, the reactive product of the hepatocarcinogen AFB1, is stable in aprotic solvents but hydrolyzes rapidly in H2O at 25 degrees C and pH 7 (t1/2 = 1 s). However, it is also known that some glutathione (GSH) transferases can conjugate the epoxide with GSH to give the adduct in high yie...

journal_title：Biochemistry

authors： Johnson WW,Ueng YF,Widersten M,Mannervik B,Hayes JD,Sherratt PJ,Ketterer B,Guengerich FP

更新日期：1997-03-18 00:00:00

abstract：:Succinyl-CoA synthetase of Escherichia coli is an alpha 2 beta 2 protein containing active sites at the interfaces between alpha- and beta-subunits. The alpha-subunit contains a histidine residue that is phosphorylated during the reaction. The beta-subunit binds coenzyme A and probably succinate [see Nishimura, J. S. ...

journal_title：Biochemistry

authors： Ybarra J,Prasad AR,Nishimura JS

更新日期：1986-11-04 00:00:00

abstract：:Studies on the structure-function relationship of transporters require the availability of sufficient amounts of the protein in a functional state. In this paper, we report the functional expression, purification, and reconstitution of the human sodium/d-glucose cotransporter1 (hSGLT1) in Pichia pastoris and ligand-in...

journal_title：Biochemistry

authors： Tyagi NK,Goyal P,Kumar A,Pandey D,Siess W,Kinne RK

更新日期：2005-11-29 00:00:00

abstract：:Drug efflux and enzymatic drug degradation are two cellular mechanisms that contribute to drug resistance in many cancers. Herein, we report the synthesis and in vitro activity of a pro-immunostimulant that exploits both processes in tandem to selectively confer cancer-mediated immunogenicity. We demonstrate that an i...

journal_title：Biochemistry

authors： Burt AJ,Hantho JD,Nielsen AE,Mancini RJ

更新日期：2018-04-17 00:00:00

abstract：:We have prepared the following cadmium-113-substituted derivatives of bovine superoxide dismutase and recorded the nuclear magnetic resonance (NMR) spectrum of the cadmium: 2Cd(II), in which Cd(II) is presumed to bind to the Zn(II) site and the copper site is unoccupied, and 2Cd(II)--2Cu(I), which is analogous to the ...

journal_title：Biochemistry

authors： Bailey DB,Ellis PD,Fee JA

更新日期：1980-02-05 00:00:00

abstract：:Shiga toxin is a bacterial protein composed of one A and five B subunits. Its A chain possesses a protease sensitive loop (Cys-242-Cys-261) that is cleaved to produce an enzymatically active A1 domain and an A2 fragment associated with its B subunit pentamer. The proposed mode of action of the toxin is linked to its r...

journal_title：Biochemistry

authors： Saleh MT,Ferguson J,Boggs JM,Gariépy J

更新日期：1996-07-23 00:00:00

abstract：:Uniform double labeling of proteins for NMR studies can be prohibitively expensive, even with an efficient expression and purification scheme, due largely to the high cost of [13C6, 99%]glucose. We demonstrate here that uniformly (greater than 95%) 13C and 15N double-labeled proteins can be prepared for NMR structure/...

journal_title：Biochemistry

authors： Venters RA,Calderone TL,Spicer LD,Fierke CA

更新日期：1991-05-07 00:00:00

abstract：:Signal transduction in B cells is mediated, in part, by the interaction of the cytoplasmic components of the antigen receptor complex and various members of the src family tyrosine kinases. Key to this process appears to be the interaction of the tyrosine kinase SH2 domains with the tyrosine-phosphorylated cytoplasmic...

journal_title：Biochemistry

authors： Metzler WJ,Leiting B,Pryor K,Mueller L,Farmer BT 2nd

更新日期：1996-05-21 00:00:00

abstract：:Plasmids containing a site-specifically incorporated O6-methyl- (m6G), O6-ethyl- (e6G), or O6-benzylguanine (b6G) within the ATG initiation codon of the lacZ' gene were used to transform Escherichia coli that were repair proficient or deficient in one or both of the E. coli O6-alkylguanine-DNA alkyltransferases, the u...

journal_title：Biochemistry

authors： Pauly GT,Hughes SH,Moschel RC

更新日期：1994-08-09 00:00:00

abstract：:Purine nucleoside phosphorylase (PNP) catalyzes the reversible phosphorolysis of purine ribonucleosides and 2'-deoxyribonucleosides, yielding the purine base and (2'-deoxy)ribose 1-phosphate as products. While this enzyme has been extensively studied, several questions with respect to the catalytic mechanism have rema...

journal_title：Biochemistry

authors： Isaksen GV,Hopmann KH,Åqvist J,Brandsdal BO

更新日期：2016-04-12 00:00:00

abstract：:The structure of cytochrome f includes an internal chain of five water molecules and six hydrogen-bonding side chains, which are conserved throughout the phylogenetic range of photosynthetic organisms from higher plants, algae, and cyanobacteria. The in vivo electron transfer capability of Chlamydomonas reinhardtii cy...

journal_title：Biochemistry

authors： Sainz G,Carrell CJ,Ponamarev MV,Soriano GM,Cramer WA,Smith JL

更新日期：2000-08-08 00:00:00