当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Characterization of the oxycomplex of lignin peroxidases from Phanerochaete chrysosporium: equilibrium and kinetics studies.

Characterization of the oxycomplex of lignin peroxidases from Phanerochaete chrysosporium: equilibrium and kinetics studies.

Abstract：

:The oxycomplexes (compound III, oxyperoxidase) of two lignin peroxidase isozymes, H1 (pI = 4.7) and H8 (pI = 3.5), were characterized in the present study. After generation of the ferroperoxidase by photochemical reduction with deazoflavin in the presence of EDTA, the oxycomplex is formed by mixing ferroperoxidase with O2. The oxycomplex of isozyme H8 is very stable, with an autoxidation rate at 25 degrees C too slow to measure at pH 3.5 or 7.0. In contrast, the oxycomplex of isozyme H1 has a half-life of 52 min at pH 4.5 and 29 min at pH 7.5 at 25 degrees C. The decay of isozyme H1 oxycomplex follows a single exponential. The half-lives of lignin peroxidase oxycomplexes are much longer than those observed with other peroxidases. The binding of O2 to ferroperoxidase to form the oxycomplex was studied by stopped-flow methods. At 20 degrees C, the second-order rate constants for O2 binding are 2.3 X 10(5) and 8.9 X 10(5) M-1 s-1 for isozyme H1 and 6.2 X 10(4) and 3.5 X 10(5) M-1 s-1 for isozyme H8 at pH 3.6 and pH 6.8, respectively. The dissociation rate constants for the oxycomplex of isozyme H1 (3.8 Z 10(-3) s-1) and isozyme H8 (1.0 X 10(-3) s-1) were measured at pH 3.6 by CO trapping. Thus, the equilibrium constants (K, calculated from kon/koff) for both isozymes H1 (7.0 X 10(7) M-1) and H8 (6.2 X 10(7) M-1) are higher than that of myoglobin (1.9 Z 10(6) M-1).(ABSTRACT TRUNCATED AT 250 WORDS)

journal_name

Biochemistry

journal_title

Biochemistry

authors

Cai DY,Tien M

doi

10.1021/bi00460a018

subject

Has Abstract

pub_date

1990-02-27 00:00:00

pages

2085-91

issue

8

eissn

0006-2960

issn

1520-4995

journal_volume

29

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Mapping global angular transitions of proteins in assemblies using multiple extrinsic reporter groups.

    abstract：:The fluorescence polarization intensities from fluorescent probes and the electron paramagnetic resonance spectra from spin probes, specifically modifying elements of a biological assembly such as myosin sulfhydryl 1 (SH1) in muscle fibers, are interpreted in terms of probe order parameters using a model-independent m...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00116a029

    authors： Burghardt TP,Ajtai K

    更新日期：1992-01-14 00:00:00

  • Structure-Guided Analyses of a Key Enzyme Involved in the Biosynthesis of an Antivitamin.

    abstract：:RosB catalyzes the formation of 8-aminoriboflavin 5'-phosphate (AFP), the key intermediate in roseoflavin biosynthesis, from the metabolic precursors riboflavin 5'-phosphate (RP, also known as FMN) and glutamate. The conversion of the aromatic methyl group at position 8 in RP into the aromatic amine in AFP occurs via ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b00576

    authors： Kapoor I,Nair SK

    更新日期：2018-09-11 00:00:00

  • Structural elucidation of a hydrophobic box in bovine alpha-lactalbumin by NMR: nuclear Overhauser effects.

    abstract：:The proton nuclear Overhauser effects of bovine alpha-lactalbumin were studied at 200 MHz by irradiation of an upfield ring current shifted methylene at -2.45 ppm (assigned to Ile-95) and two aromatic protons, Tyr-103 (8.36 ppm) and Trp-60 (5.85 ppm). The experimental results were consistent with a putative three-dime...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00346a035

    authors： Koga K,Berliner LJ

    更新日期：1985-12-03 00:00:00

  • Mechanistic characterization of the bifunctional aminoglycoside-modifying enzyme AAC(3)-Ib/AAC(6')-Ib' from Pseudomonas aeruginosa.

    abstract：:A recently discovered bifunctional antibiotic-resistance enzyme named AAC(3)-Ib/AAC(6')-Ib', from Pseudomonas aeruginosa, catalyzes acetylation of aminoglycoside antibiotics. Since both domains are acetyltransferases, each was cloned and purified for mechanistic studies. The AAC(3)-Ib domain appears to be highly speci...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi700111z

    authors： Kim C,Villegas-Estrada A,Hesek D,Mobashery S

    更新日期：2007-05-01 00:00:00

  • Structure-based stability analysis of an extremely stable dimeric DNA binding protein from Sulfolobus islandicus.

    abstract：:ORF56 is a small and thermodynamically extremely stable dimeric protein from the archaeon Sulfolobus islandicus. This DNA binding protein is encoded on plasmid pRN1 and possibly controls the copy number of the plasmid. We report the solution NMR structure as well as the crystal structure of ORF56 comprising a ribbon-h...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi900760n

    authors： Weininger U,Zeeb M,Neumann P,Löw C,Stubbs MT,Lipps G,Balbach J

    更新日期：2009-10-27 00:00:00

  • G-protein-dependent cell surface dynamics of the human serotonin1A receptor tagged to yellow fluorescent protein.

    abstract：:Serotonergic signaling appears to play a key role in the generation and modulation of various cognitive, behavioral, and developmental processes. The serotonin(1A) receptor is an important member of the superfamily of seven transmembrane domain G-protein-coupled receptors and is the most extensively studied among the ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0480887

    authors： Pucadyil TJ,Kalipatnapu S,Harikumar KG,Rangaraj N,Karnik SS,Chattopadhyay A

    更新日期：2004-12-21 00:00:00

  • Thermodynamic characterization of the binding interaction between the histone demethylase LSD1/KDM1 and CoREST.

    abstract：:Flavin-dependent histone demethylases catalyze the posttranslational oxidative demethylation of mono- and dimethylated lysine residues, producing formaldehyde and hydrogen peroxide in addition to the corresponding demethylated protein. In vivo, histone demethylase LSD1 (KDM1; BCH110) is a component of the multiprotein...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi101776t

    authors： Hwang S,Schmitt AA,Luteran AE,Toone EJ,McCafferty DG

    更新日期：2011-02-01 00:00:00

  • The transcription regulator RcoM-2 from Burkholderia xenovorans is a cysteine-ligated hemoprotein that undergoes a redox-mediated ligand switch.

    abstract：:Spectroscopic characterization of the newly discovered heme-PAS domain sensor protein BxRcoM-2 reveals that this protein undergoes redox-dependent ligand switching and CO- and NO-induced ligand displacement. The aerobic bacterium Burkholderia xenovorans expresses two homologous heme-containing proteins that promote CO...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi800486x

    authors： Marvin KA,Kerby RL,Youn H,Roberts GP,Burstyn JN

    更新日期：2008-08-26 00:00:00

  • Investigation of the role and mechanism of IF1 and STF1 proteins, twin inhibitory peptides which interact with the yeast mitochondrial ATP synthase.

    abstract：:Inhibition of the yeast F(0)F(1)-ATP synthase by the regulatory peptides IF1 and STF1 was studied using intact mitochondria and submitochondrial particles from wild-type cells or from mutants lacking one or both peptides. In intact mitochondria, endogenous IF1 only inhibited uncoupled ATP hydrolysis and endogenous STF...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi034394t

    authors： Venard R,Brèthes D,Giraud MF,Vaillier J,Velours J,Haraux F

    更新日期：2003-06-24 00:00:00

  • Aspartate-histidine interaction in the retinal schiff base counterion of the light-driven proton pump of Exiguobacterium sibiricum.

    abstract：:One of the distinctive features of eubacterial retinal-based proton pumps, proteorhodopsins, xanthorhodopsin, and others, is hydrogen bonding of the key aspartate residue, the counterion to the retinal Schiff base, to a histidine. We describe properties of the recently found eubacterium proton pump from Exiguobacteriu...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi300409m

    authors： Balashov SP,Petrovskaya LE,Lukashev EP,Imasheva ES,Dioumaev AK,Wang JM,Sychev SV,Dolgikh DA,Rubin AB,Kirpichnikov MP,Lanyi JK

    更新日期：2012-07-24 00:00:00

  • Insights into Cu(I) exchange in HAH1 using quantum mechanical and molecular simulations.

    abstract：:The human antioxidant protein, HAH1, is an important participant in a Cu(I) transport chain, delivering one Cu(I) ion to the Wilson's (WND) or Menkes disease protein (MNK). Full geometry optimizations and second-derivative calculations were performed on several binding site models using the B3LYP functional to derive ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi7007195

    authors： Op't Holt BT,Merz KM Jr

    更新日期：2007-07-31 00:00:00

  • Effects of a distal mutation on active site chemistry.

    abstract：:Previous studies of Escherichia coli dihydrofolate reductase (ecDHFR) have demonstrated that residue G121, which is 19 A from the catalytic center, is involved in catalysis, and long distance dynamical motions were implied. Specifically, the ecDHFR mutant G121V has been extensively studied by various experimental and ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0518242

    authors： Wang L,Tharp S,Selzer T,Benkovic SJ,Kohen A

    更新日期：2006-02-07 00:00:00

  • Electron spin echo envelope modulation study of oxygenated iron-cobalt hybrid hemoglobins reveals molecular features analogous to those of the oxy ferrous protein.

    abstract：:Two oxygenated iron-cobalt hybrid hemoglobins (Hbs), (alpha Co-O2 beta Fe-O2)2 and (alpha Fe-O2 beta Co-O2)2, were studied by electron spin echo envelope modulation (ESEEM) spectroscopy in order to measure (i) electron-nuclear hyperfine and nuclear quadrupole coupling to the N epsilon of the proximal histidyl imidazol...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00020a036

    authors： Lee HC,Peisach J,Tsuneshige A,Yonetani T

    更新日期：1995-05-23 00:00:00

  • Exchange rates at the lipid-protein interface of myelin proteolipid protein studied by spin-label electron spin resonance.

    abstract：:The electron spin resonance (ESR) spectra from spin-labeled phospholipids in recombinants of myelin proteolipid apoprotein with dimyristoylphosphatidylcholine have been simulated with the exchanged-coupled Bloch equations to obtain values for both the fraction of motionally restricted lipids and the exchange rate betw...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00401a009

    authors： Horváth LI,Brophy PJ,Marsh D

    更新日期：1988-01-12 00:00:00

  • Fluidity of sarcoplasmic reticulum membranes investigated with dipyrenylpropane, an intramolecular excimer probe.

    abstract：:Intramolecular excimer formation with 1,3-di(1-pyrenyl)propane was used to probe the fluidity of sarcoplasmic reticulum (SR) membranes where the probe could be incorporated (in a probe/lipid molar ratio of 1/2000 or less) without inducing any detectable damage. The temperature was varied between -1 and 40 degrees C. T...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00266a038

    authors： Almeida LM,Vaz WL,Zachariasse KA,Madeira VM

    更新日期：1982-11-09 00:00:00

  • Altering the reaction specificity of eukaryotic ornithine decarboxylase.

    abstract：:Ornithine decarboxylase (ODC) catalyzes the first committed step in the biosynthesis of polyamines, and it has been identified as a drug target for the treatment of African sleeping sickness, caused by Trypanosoma brucei. ODC is a pyridoxal 5'-phosphate (PLP) dependent enzyme and an obligate homodimer. X-ray structura...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi001209s

    authors： Jackson LK,Brooks HB,Osterman AL,Goldsmith EJ,Phillips MA

    更新日期：2000-09-19 00:00:00

  • Arginine294 is essential for the inhibition of Anabaena PCC 7120 ADP-glucose pyrophosphorylase by phosphate.

    abstract：:Treatment of ADP-glucose pyrophosphorylase (EC 2.7.7.27) from the cyanobacterium Anabaena PCC 7120 with phenylglyoxal in 50 mM Hepes, pH 8.0, at 25 degrees C resulted in a time- and concentration-dependent loss of enzyme activity. Phosphate, the inhibitor, protected the enzyme from inactivation most effectively, while...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9713355

    authors： Sheng J,Preiss J

    更新日期：1997-10-21 00:00:00

  • Interaction of assembly protein AP-2 and its isolated subunits with clathrin.

    abstract：:The clathrin assembly protein complex AP-2 is a multimeric subunit complex consisting of two 100-115-kDa subunits known as alpha and beta and 50- and 16-kDa subunits. The subunits have been dissociated and separated by ion-exchange chromatography in 7.5 M urea. Fractions highly enriched in either the alpha or beta sub...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00236a036

    authors： Prasad K,Keen JH

    更新日期：1991-06-04 00:00:00

  • Insight into the mechanism of inactivation of ribonucleotide reductase by gemcitabine 5'-diphosphate in the presence or absence of reductant.

    abstract：:Gemcitabine 5'-diphosphate (F(2)CDP) is a potent inhibitor of ribonucleotide reductases (RNRs), enzymes that convert nucleotides (NDPs) to deoxynucleotides and are essential for DNA replication and repair. The Escherichia coli RNR, an alpha2beta2 complex, when incubated with 1 equiv of F(2)CDP catalyzes the release of...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi901590q

    authors： Artin E,Wang J,Lohman GJ,Yokoyama K,Yu G,Griffin RG,Bar G,Stubbe J

    更新日期：2009-12-15 00:00:00

  • Spectroscopic Determination of Distinct Heme Ligands in Outer-Membrane Receptors PhuR and HasR of Pseudomonas aeruginosa.

    abstract：:Pseudomonas aeruginosa PAO1 encodes two outer membrane receptors, PhuR (Pseudomonas heme uptake) and HasR (heme assimilation system). The HasR receptor acquires heme through interaction with a secreted hemophore, HasAp. The non-hemophore-dependent PhuR is encoded along with proteins required for heme translocation int...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b00017

    authors： Smith AD,Modi AR,Sun S,Dawson JH,Wilks A

    更新日期：2015-04-28 00:00:00

  • Characterization of human glucocorticoid receptor complexes formed with DNA fragments containing or lacking glucocorticoid response elements.

    abstract：:Sucrose density gradient shift assays were used to study the interactions of human glucocorticoid receptors (GR) with small DNA fragments either containing or lacking glucocorticoid response element (GRE) DNA consensus sequences. When crude cytoplasmic extracts containing [3H]triamcinolone acetonide [( 3H]TA) labeled ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00431a003

    authors： Tully DB,Cidlowski JA

    更新日期：1989-03-07 00:00:00

  • DNA Oligonucleotide 3'-Phosphorylation by a DNA Enzyme.

    abstract：:T4 polynucleotide kinase is widely used for 5'-phosphorylation of DNA and RNA oligonucleotide termini, but no natural protein enzyme is capable of 3'-phosphorylation. Here, we report the in vitro selection of deoxyribozymes (DNA enzymes) capable of DNA oligonucleotide 3'-phosphorylation, using a 5'-triphosphorylated R...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.6b00151

    authors： Camden AJ,Walsh SM,Suk SH,Silverman SK

    更新日期：2016-05-10 00:00:00

  • Site-directed mutagenesis in hemoglobin: functional and structural role of inter- and intrasubunit hydrogen bonds as studied with 37 beta and 145 beta mutations.

    abstract：:In order to clarify the functional and structural role of intra- and intersubunit hydrogen bonds in human hemoglobin (Hb A), we prepared two artificial beta chain mutant hemoglobins by site-directed mutagenesis. The mutant Hb Phe-37 beta, in which Trp-37 beta is replaced by Phe to remove the intersubunit hydrogen bond...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00127a030

    authors： Ishimori K,Imai K,Miyazaki G,Kitagawa T,Wada Y,Morimoto H,Morishima I

    更新日期：1992-03-31 00:00:00

  • Tyr51: Key Determinant of the Low Thermostability of the Colwellia psychrerythraea Cold-Shock Protein.

    abstract：:Cold-shock proteins (Csps) are expressed at lower-than-optimum temperatures, and they function as RNA chaperones; however, no structural studies on psychrophilic Csps have been reported. Here, we aimed to investigate the structure and dynamics of the Csp of psychrophile Colwellia psychrerythraea 34H, ( Cp-Csp). Althou...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b00144

    authors： Lee Y,Kwak C,Jeong KW,Durai P,Ryu KS,Kim EH,Cheong C,Ahn HC,Kim HJ,Kim Y

    更新日期：2018-07-03 00:00:00

  • Inhibition of mouse erythroid band 3-mediated chloride transport by site-directed mutagenesis of histidine residues and its reversal by second site mutation of Lys 558, the locus of covalent H2DIDS binding.

    abstract：:Substitution by site-directed mutagenesis of any one of the histidine residues H721, H837, and H852 by glutamine, or of H752 by serine, inhibits Cl- flux mediated by band 3 expressed in Xenopus oocytes. Mutation of Lys 558 (K558N), the site of covalent binding of H2DIDS (4,4'-diisothiocyanostilbene-2,2'-disulfonate) i...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00029a006

    authors： Müller-Berger S,Karbach D,König J,Lepke S,Wood PG,Appelhans H,Passow H

    更新日期：1995-07-25 00:00:00

  • Physical and conformational properties of synthetic idealized signal sequences parallel their biological function.

    abstract：:Transported proteins often contain an extension sequence called the signal peptide. The alkaline phosphatase (PhoA) signal sequence represents a typical signal peptide for comparison to idealized sequences both in vivo and in vitro. We have designed a series of idealized signal sequences which vary in amino terminal c...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00031a012

    authors： Izard JW,Doughty MB,Kendall DA

    更新日期：1995-08-08 00:00:00

  • Light-chain-independent binding of adaptors, AP180, and auxilin to clathrin.

    abstract：:Binding of coated vesicle assembly proteins to clathrin causes it to assemble into regular coat structures. The assembly protein fraction of bovine brain coated vesicles comprises AP180, auxilin, and HA1 and HA2 adaptors. Clathrin heavy chains, separated from their light chains, polymerize with unimpaired efficiency w...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00101a027

    authors： Lindner R,Ungewickell E

    更新日期：1991-09-17 00:00:00

  • Understanding the mechanism of beta-hairpin folding via phi-value analysis.

    abstract：:The folding kinetics of a 16-residue beta-hairpin (trpzip4) and five mutants were studied by a laser-induced temperature-jump infrared method. Our results indicate that mutations which affect the strength of the hydrophobic cluster lead to a decrease in the thermal stability of the beta-hairpin, as a result of increas...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi052039s

    authors： Du D,Tucker MJ,Gai F

    更新日期：2006-02-28 00:00:00

  • Lipidic sponge phase crystal structure of a photosynthetic reaction center reveals lipids on the protein surface.

    abstract：:Membrane proteins are embedded in a lipid bilayer and maintain strong interactions with lipid molecules. Tightly bound lipids are responsible for vertical positioning and integration of proteins in the membrane and for assembly of multisubunit complexes and occasionally act as substrates. In this work we present the l...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi900545e

    authors： Wöhri AB,Wahlgren WY,Malmerberg E,Johansson LC,Neutze R,Katona G

    更新日期：2009-10-20 00:00:00

  • Crystal structure of rat apo-heme oxygenase-1 (HO-1): mechanism of heme binding in HO-1 inferred from structural comparison of the apo and heme complex forms.

    abstract：:Heme oxygenase (HO) catalyzes the oxidative cleavage of heme to biliverdin by utilizing O(2) and NADPH. HO (apoHO) was crystallized as twinned P3(2) with three molecules per asymmetric unit, and its crystal structure was determined at 2.55 A resolution. Structural comparison of apoHO and its complex with heme (HO-heme...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi025662a

    authors： Sugishima M,Sakamoto H,Kakuta Y,Omata Y,Hayashi S,Noguchi M,Fukuyama K

    更新日期：2002-06-11 00:00:00