Abstract:
:Binding of coated vesicle assembly proteins to clathrin causes it to assemble into regular coat structures. The assembly protein fraction of bovine brain coated vesicles comprises AP180, auxilin, and HA1 and HA2 adaptors. Clathrin heavy chains, separated from their light chains, polymerize with unimpaired efficiency when assembly proteins are added. The reassembled coats were purified by sucrose gradient centrifugation and examined for composition by SDS-PAGE and immunoblotting. We found that all four major coat proteins are incorporated in the presence and absence of light chains. Moreover, each of the purified coat proteins is able to associate directly with clathrin heavy chains in preassembled cages as efficiently as with intact clathrin. We conclude that light chains are not essential for the interaction of AP180, auxilin, and HA1 and HA2 with clathrin.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Lindner R,Ungewickell Edoi
10.1021/bi00101a027subject
Has Abstractpub_date
1991-09-17 00:00:00pages
9097-101issue
37eissn
0006-2960issn
1520-4995journal_volume
30pub_type
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