Cryoenzymic studies on actomyosin ATPase. Evidence that the degree of saturation of actin with myosin subfragment 1 affects the kinetics of the binding of ATP.

abstract：:The type III secretion system (T3SS) is an essential virulence factor for Shigella flexneri , providing a conduit through which host-altering effectors are injected directly into a host cell to promote uptake. The type III secretion apparatus (T3SA) is composed of a basal body, external needle, and regulatory tip comp...

journal_title：Biochemistry

authors： Dickenson NE,Arizmendi O,Patil MK,Toth RT 4th,Middaugh CR,Picking WD,Picking WL

更新日期：2013-12-10 00:00:00

abstract：:31P nuclear magnetic resonance (NMR) saturation-transfer (ST) techniques have been used to measure steady-state flows through phosphate-adenosine 5'-triphosphate (ATP) exchange reactions in glucose-grown derepressed yeast. Our results have revealed that the reactions catalyzed by glyceraldehyde-3-phosphate dehydrogena...

journal_title：Biochemistry

authors： Campbell-Burk SL,Jones KA,Shulman RG

更新日期：1987-11-17 00:00:00

abstract：:We have investigated some properties related to interaction with DNA and recognition of AT-rich sequences of netropsin-oxazolopyridocarbazole (Net-OPC) (Mrani et al., 1990), which is a hybrid groove-binder-intercalator. The hybrid molecule Net-OPC binds to poly[d(A-T)] at two different sites with Kapp values close to ...

journal_title：Biochemistry

authors： Subra F,Carteau S,Pager J,Paoletti J,Paoletti C,Auclair C,Mrani D,Gosselin G,Imbach JL

更新日期：1991-02-12 00:00:00

abstract：:The crystallographic structure of oxygenated trHbN from Mycobacterium tuberculosis showed an extended heme distal site hydrogen-bonding network that includes Y(B10), Q(E11), and the bound O(2) (Milani, M., et al. (2001) EMBO J. 20, 3902-3909). In the present work, we analyze the effects that substitutions at the B10 a...

journal_title：Biochemistry

authors： Ouellet Y,Milani M,Couture M,Bolognesi M,Guertin M

更新日期：2006-07-25 00:00:00

abstract：:Previous studies of the low molecular mass family 11 xylanase from Bacillus circulans show that the ionization state of the nucleophile (Glu78, pK(a) 4.6) and the acid/base catalyst (Glu172, pK(a) 6.7) gives rise to its pH-dependent activity profile. Inspection of the crystal structure of BCX reveals that Glu78 and Gl...

journal_title：Biochemistry

authors： Joshi MD,Sidhu G,Nielsen JE,Brayer GD,Withers SG,McIntosh LP

更新日期：2001-08-28 00:00:00

abstract：:The molecular weight of phosphophoryn, an acidic phosphoprotein unique to dentin matrix, has been difficult to determine because of a combination of neutral protease activities in this tissue and the intrinsic high charge density of the molecule. In this study, bovine dentin phosphophoryn (BDPP) was isolated by a proc...

journal_title：Biochemistry

authors： Stetler-Stevenson WG,Veis A

更新日期：1983-08-30 00:00:00

abstract：:Phosphorus NMR spectroscopy is an important technique for the investigation of metabolism in tissues and intact organisms (including man). However, quantitation of the signals from an NMR experiment is difficult because it is not known from which regions of a cell metabolites are detected. It is generally believed tha...

journal_title：Biochemistry

authors： Jeffrey FM,Storey CJ,Nunnally RL,Malloy CR

更新日期：1989-06-27 00:00:00

abstract：:The ATP analogue 5'-[p-(fluorosulfonyl)benzoyl]adenosine (FSBA) was used to chemically modify the ATP binding sites of the carbamyl phosphate synthetase domain of CAD, the multifunctional protein that catalyzes the first steps in mammalian pyrimidine biosynthesis. Reaction of CAD with FSBA resulted in the inactivation...

journal_title：Biochemistry

authors： Kim HS,Lee L,Evans DR

更新日期：1991-10-22 00:00:00

abstract：:Human Monocyte Chemotactic Protein (MCP)-2 has originally been isolated from stimulated osteosarcoma cells as a chemokine coproduced with MCP-1 and MCP-3. Here, a 5'-end extended MCP-2 cDNA was cloned from a human testis cDNA library. It encoded a 76 residue MCP-2 protein, but differed from the reported bone marrow-de...

journal_title：Biochemistry

authors： Van Coillie E,Proost P,Van Aelst I,Struyf S,Polfliet M,De Meester I,Harvey DJ,Van Damme J,Opdenakker G

更新日期：1998-09-08 00:00:00

abstract：:4-Hydroxybenzoyl-CoA reductase (4-HBCR) is a member of the xanthine oxidase (XO) family of molybdenum cofactor containing enzymes and catalyzes the irreversible removal of a phenolic hydroxy group by reduction, yielding benzoyl-CoA and water. In this work the effects of various activity modulating compounds were chara...

journal_title：Biochemistry

authors： Johannes J,Unciuleac MC,Friedrich T,Warkentin E,Ermler U,Boll M

更新日期：2008-04-29 00:00:00

abstract：:Phospholipids containing a saturated fatty acid in the primary position and an unsaturated fatty acid in the secondary position are a major structural part of biological membranes. The mixed-chain hydrophobic core of the membranes is the diacylglycerol part. To better understand the core properties of membranes we hav...

journal_title：Biochemistry

authors： Di L,Small DM

更新日期：1995-12-26 00:00:00

abstract：:We have measured the pH-dependent (1)H, (13)C, and (15)N NMR spectra of pyridoxal 5'-phosphate ((13)C(2)-PLP) mixed with equal amounts of either doubly (15)N-labeled diaminopropane, (15)N(α)-labeled l-lysine, or (15)N(ε)-labeled l-lysine as model systems for various intermediates of the transimination reaction in PLP-...

journal_title：Biochemistry

authors： Chan-Huot M,Sharif S,Tolstoy PM,Toney MD,Limbach HH

更新日期：2010-12-28 00:00:00

abstract：:The R2 protein of ribonucleotide reductase catalyzes the dioxygen-dependent one-electron oxidation of Tyr122 at a diiron-carboxylate site. Methane monooxygenase and related hydroxylases catalyze hydrocarbon hydroxylation at diiron sites structurally related to the one in R2. In protein R2, the likely reaction site for...

journal_title：Biochemistry

authors： Logan DT,deMaré F,Persson BO,Slaby A,Sjöberg BM,Nordlund P

更新日期：1998-07-28 00:00:00

abstract：:The histone content of zinc-deficient (-Zn) Euglena gracilis decreases while, concomitantly, DNA content increases and the transcription rate is reduced markedly [Mazus, B., Falchuk, K. H., & Vallee, B. L. (1983) Biochemistry (in press); Falchuk, K. H., Fawcett, D. W., & Vallee, B. L. (1975) J. Cell Sci. 17, 57-78]. T...

journal_title：Biochemistry

authors： Stankiewicz AJ,Falchuk KH,Vallee BL

更新日期：1983-10-25 00:00:00

abstract：:Pyridoxal 5'-triphospho-5'-adenosine (AP3-PL), the affinity labeling reagent specific for lysine residues in the nucleotide-binding site of several enzymes [Tagaya, M., & Fukui, T. (1986) Biochemistry 25, 2958-2964; Yagami, T., Tagaya, M., & Fukui, T. (1988) FEBS Lett. 229, 261-264], was used to identify the ATP-bindi...

journal_title：Biochemistry

authors： Hountondji C,Schmitter JM,Fukui T,Tagaya M,Blanquet S

更新日期：1990-12-25 00:00:00

abstract：:The anomeric proton (H-1) chemical shifts of D-mannopyranosides in aqueous solution are affected both by the aglycon and by substitution of the ring [Lee, Y. C., & Ballou, C. E. (1965) Biochemistry 4, 257]. We have examined the 1H NMR spectra for a variety of linear and branched mannooligosaccharides and have assigned...

journal_title：Biochemistry

authors： Cohen RE,Ballou CE

更新日期：1980-09-02 00:00:00

abstract：:To describe the hydrophobic adsorption of charged molecules to bilayer membranes, one must recognize that the adsorption produces a change in the electrostatic potential at the surface of the membrane. The surface potential produced by the adsorption of the charged molecules can be described most simply by the Gouy eq...

journal_title：Biochemistry

authors： McLaughlin S,Harary H

更新日期：1976-05-04 00:00:00

abstract：:PsbO, the photosystem II manganese stabilizing protein, contains an aspartate residue [Asp157 (spinach numbering)], which is highly conserved in eukaryotic and prokaryotic PsbOs. The homology model of the PSII-bound conformation of spinach PsbO presented here positions Asp157 in the large flexible loop of the protein....

journal_title：Biochemistry

authors： Popelkova H,Commet A,Yocum CF

更新日期：2009-12-22 00:00:00

abstract：:Escherichia coli 3-methyladenine DNA glycosylase II (AlkA), an adaptive response glycosylase with a broad substrate range, initiates base excision repair by flipping a lesion out of the DNA duplex and hydrolyzing the N-glycosidic bond. We used transient and steady state kinetics to determine the minimal mechanism for ...

journal_title：Biochemistry

authors： Taylor EL,O'Brien PJ

更新日期：2015-01-27 00:00:00

abstract：:Specific binding of glutamate to its carrier was investigated by using cytoplasmic membrane vesicles prepared from Escherichia coli B. The binding activity was specifically affected by the Na+ and H+ concentrations of the medium. Cytoplasmic membrane vesicles from the mutant strain 36-39 that is defective in the Na+-d...

journal_title：Biochemistry

authors： Fujimura T,Yamato I,Anraku Y

更新日期：1983-04-12 00:00:00

abstract：:Dihydroorotates deuteriated at both C5 and C6 have been prepared and used to probe the mechanism of the bovine liver mitochondrial dihydroorotate dehydrogenase. Primary deuterium isotope effects on kcat are observed with both (6RS)-[5(S)-2H]- and (6RS)-[6-2H] dihydroorotates (3 and 6, respectively); these effects are ...

journal_title：Biochemistry

authors： Hines V,Johnston M

更新日期：1989-02-07 00:00:00

abstract：:Kinesin-5 proteins are essential for formation of a bipolar mitotic spindle in most and, perhaps all, eukaryotic cells. Several Kinesin-5 proteins, notably the human version, HsEg5, are targets of a constantly expanding group of small-molecule inhibitors, which hold promise both as tools for probing mechanochemical tr...

journal_title：Biochemistry

authors： Learman SS,Kim CD,Stevens NS,Kim S,Wojcik EJ,Walker RA

更新日期：2009-03-03 00:00:00

abstract：:Blastochloris tepida is a newly described thermophilic purple bacterium containing bacteriochlorophyll b. Using purified light-harvesting 1 reaction center (LH1-RC) core complexes from Blc. tepida, we compared the biochemical, spectroscopic, and thermal denaturation properties of these complexes with those of its meso...

journal_title：Biochemistry

authors： Seto R,Takaichi S,Kurihara T,Kishi R,Honda M,Takenaka S,Tsukatani Y,Madigan MT,Wang-Otomo ZY,Kimura Y

更新日期：2020-06-30 00:00:00

abstract：:Initiation of RNA synthesis by Bacillus subtilis RNA polymerase (sigma-43) has been examined at two early promoters of phage phi 29: the A2 promoter, which is a weak promoter, and the G2 promoter, which is a strong promoter. The delta subunit of the polymerase inhibits the rate of initiation at A2, but not G2. In addi...

journal_title：Biochemistry

authors： Dobinson KF,Spiegelman GB

更新日期：1987-12-15 00:00:00

abstract：:Several fusions between the gene for human insulin-like growth factor I (IGF-I) and the genes for different IgG-binding fragments of staphylococcal protein A were assembled and compared regarding expression, secretion, and purification of the peptide hormone. After IgG affinity purification of the fusion proteins from...

journal_title：Biochemistry

authors： Moks T,Abrahmsén L,Holmgren E,Bilich M,Olsson A,Uhlén M,Pohl G,Sterky C,Hultberg H,Josephson S

更新日期：1987-08-25 00:00:00

abstract：:The propeptide binding/activation site on the vitamin K dependent carboxylase has been localized to a region of carboxylase between residues Arg +50 and Glu +225 by photoinactivation studies using [125I]tyrosyl-labeled benzoylphenylalanine (Bpa)-containing analogs of proFIX19, a peptide containing residues -18 to +1 o...

journal_title：Biochemistry

authors： Yamada M,Kuliopulos A,Nelson NP,Roth DA,Furie B,Furie BC,Walsh CT

更新日期：1995-01-17 00:00:00

abstract：:Factor XIIIa (a2') is a homodimeric transglutaminase that is formed via limited alpha-thrombin-catalyzed proteolysis of the platelet (a2) or plasma (a2b2) factor XIII zymogen in a reaction that results in proteolytic removal of a 37-aminoacyl residue peptide from the N-terminus of the a chains and exposure of the acti...

journal_title：Biochemistry

authors： Hornyak TJ,Shafer JA

更新日期：1992-01-21 00:00:00

abstract：:Four murine myeloma immunoglobulins, A4, A47N, U61, and E109, have been studied for their binding affinities with inulin and a series of oligosaccharides derived from inulin. The results indicate that the combining site of these immunoglobulins shows highest complementarity for a trifructofuranosyl sequence (A4 and A4...

journal_title：Biochemistry

authors： Streefkerk DG,Glaudemans CP

更新日期：1977-08-23 00:00:00

abstract：:The DNA binding of three platinum(II) intercalation reagents has been studied and found to depend upon base composition, the nature of the intercalator, and the ionic strength of the solvent medium. In 0.2 M NaCl, binding data for calf thymus DNA show the association constants to be approximately 10(4) M-1. The bindin...

journal_title：Biochemistry

authors： Howe-Grant M,Lippard SJ

更新日期：1979-12-25 00:00:00

abstract：:A modified form of procollagen was extracted with 10 M urea from the skin of lambs with dermatosparaxis, a disease which is produced by a genetic defect in the conversion of procollagen to collagen. The extracts contained little if any alpha1 and alpha2 chains of normal type I collagen, and instead they contained the ...

journal_title：Biochemistry

authors： Becker U,Timpl R

更新日期：1976-06-29 00:00:00