当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Response of repair-competent and repair-deficient Escherichia coli to three O6-substituted guanines and involvement of methyl-directed mismatch repair in the processing of O6-methylguanine residues.

Response of repair-competent and repair-deficient Escherichia coli to three O6-substituted guanines and involvement of methyl-directed mismatch repair in the processing of O6-methylguanine residues.

Abstract：

:Plasmids containing a site-specifically incorporated O6-methyl- (m6G), O6-ethyl- (e6G), or O6-benzylguanine (b6G) within the ATG initiation codon of the lacZ' gene were used to transform Escherichia coli that were repair proficient or deficient in one or both of the E. coli O6-alkylguanine-DNA alkyltransferases, the uvr(ABC) excision repair system, the recA-mediated recombination system, or the methylation-directed mismatch repair system. Colonies were scored phenotypically for adduct-induced mutations. With plasmids containing either e6G or b6G, the frequency of adduct-induced mutation was low and independent of the repair proficiency of the strain transformed. Plasmids containing an m6G residue elicited similar responses in all but the mismatch repair-deficient strain. The generally low mutagenicity of all the O6-substituted guanines was interpreted as reflecting an adduct-induced arrest of replication of the modified strand while the unmodified complementary strand was replicated normally. Studies of the involvement of mismatch repair in m6G mutagenesis showed that m6G:T base pairs were more readily processed than m6G:C base pairs, indicating that mismatch repair involving m6G residues occurs after replication. These data support a model in which the E. coli methylation-directed mismatch repair system diverts plasmids containing promutagenic m6G:T base pairs into replication-arrested complexes providing another line of defense against O6-methylguanine mutagenicity in addition to O6-alkylguanine-DNA alkyltransferase repair and excision repair mechanisms.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Pauly GT,Hughes SH,Moschel RC

doi

10.1021/bi00197a020

subject

Has Abstract

pub_date

1994-08-09 00:00:00

pages

9169-77

issue

31

eissn

0006-2960

issn

1520-4995

journal_volume

33

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • A transition path ensemble study reveals a linchpin role for Mg(2+) during rate-limiting ADP release from protein kinase A.

    abstract：:Protein kinases are key regulators of diverse signaling networks critical for growth and development. Protein kinase A (PKA) is an important kinase prototype that phosphorylates protein targets at Ser and Thr residues by converting ATP to ADP. Mg(2+) ions play a crucial role in regulating phosphoryl transfer and can l...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi901475g

    authors： Khavrutskii IV,Grant B,Taylor SS,McCammon JA

    更新日期：2009-12-08 00:00:00

  • Phospholipids chiral at phosphorus. Dramatic effects of phosphorus chirality on the deuterium NMR properties of the choline head group of phospholipids in the liquid crystalline phase.

    abstract：:To probe the motional and conformational properties of the choline head group of 1,2-dipalmitoyl-sn-glycero-3-thiophosphocholine (DPPsC), the Rp, Sp, and Rp + Sp isomers of [alpha-D2]DPPsC, [beta-D2]DPPsC, and [delta-D9]DPPsC in the subgel, gel, and liquid crystalline phases were investigated with deuterium NMR, and t...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00501a008

    authors： Loffredo WM,Jiang RT,Tsai MD

    更新日期：1990-12-11 00:00:00

  • Conformational changes of HIV reverse transcriptase subunits on formation of the heterodimer: correlation with kcat and Km.

    abstract：:The reverse transcriptase (RT) from the human immunodeficiency virus (HIV) is initially expressed as a 66-kDa protein and is subsequently proteolytically processed in vivo to form a 66-kDa/51-kDa heterodimer. Comparison of circular dichroism spectra of the 66-kDa, 51-kDa, and heterodimeric forms of RT indicates that t...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00150a015

    authors： Anderson SF,Coleman JE

    更新日期：1992-09-08 00:00:00

  • A loop between transmembrane helices IX and X of subunit I of cytochrome c oxidase caps the heme a-heme a3-CuB center.

    abstract：:Site-directed mutants were prepared of four consecutive and highly conserved residues (His-411, Asp-412, Thr-413, Tyr-414) of an extramembrane loop that connects putative transmembrane helices IX and X of subunit I of Rhodobacter sphaeroides cytochrome c oxidase. The modified enzymes were purified and analyzed by opti...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00171a019

    authors： Hosler JP,Shapleigh JP,Tecklenburg MJ,Thomas JW,Kim Y,Espe M,Fetter J,Babcock GT,Alben JO,Gennis RB

    更新日期：1994-02-08 00:00:00

  • Role of mitochondrial and cytoplasmic serine hydroxymethyltransferase isozymes in de novo purine synthesis in Saccharomyces cerevisiae.

    abstract：:One-carbon units are essential to a variety of anabolic processes which yield necessary cellular components including purines, pyrimidines, amino acids, and lipids. Serine hydroxymethyltransferase (SHMT) is the major provider of one-carbon units in the cell. The other product of this reaction is glycine. Both of these...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi971610n

    authors： Kastanos EK,Woldman YY,Appling DR

    更新日期：1997-12-02 00:00:00

  • Sterol carrier protein-2 functions in phosphatidylinositol transfer and signaling.

    abstract：:Over 20 years ago, it was reported that liver cytosol contains at least two distinct proteins that transfer phosphatidylinositol in vitro, phosphatidylinositol transfer protein (PITP) and a pH 5.1 supernatant fraction containing sterol carrier protein-2 (SCP-2). In contrast to PITP, there has been minimal progress on ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi026904+

    authors： Schroeder F,Zhou M,Swaggerty CL,Atshaves BP,Petrescu AD,Storey SM,Martin GG,Huang H,Helmkamp GM,Ball JM

    更新日期：2003-03-25 00:00:00

  • Generation and Comparative Kinetic Analysis of New Glycosynthase Mutants from Streptococcus pyogenes Endoglycosidases for Antibody Glycoengineering.

    abstract：:Chemoenzymatic glycan remodeling by endoglycosidase-catalyzed deglycosylation and reglycosylation is emerging as an attractive approach for producing homogeneous glycoforms of antibodies, and the success of this approach depends on the discovery of efficient endoglycosidases and their glycosynthase mutants. We report ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b00719

    authors： Tong X,Li T,Li C,Wang LX

    更新日期：2018-09-04 00:00:00

  • The R163K mutant of human thymidylate synthase is stabilized in an active conformation: structural asymmetry and reactivity of cysteine 195.

    abstract：:Loop 181-197 of human thymidylate synthase (hTS) populates two conformational states. In the first state, Cys195, a residue crucial for catalytic activity, is in the active site (active conformer); in the other conformation, it is about 10 A away, outside the active site (inactive conformer). We have designed and expr...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi7019386

    authors： Gibson LM,Lovelace LL,Lebioda L

    更新日期：2008-04-22 00:00:00

  • A hydroxyl group at residue 216 is essential for catalysis by human thymidylate synthase.

    abstract：:Structural analyses of bacterial thymidylate synthases (TSs) implicate a serine residue corresponding to Ser216 in human TS in hydrogen bond networks that are involved in binding of the nucleotide substrate, 2'-deoxyuridylate (dUMP), and that stabilize a beta-bulge in the protein. Utilizing site-directed mutagenesis, ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi972562+

    authors： Williams AW,Dunlap RB,Berger SH

    更新日期：1998-05-19 00:00:00

  • Tuning the Binding Affinities and Reversion Kinetics of a Light Inducible Dimer Allows Control of Transmembrane Protein Localization.

    abstract：:Inducible dimers are powerful tools for controlling biological processes through colocalizing signaling molecules. To be effective, an inducible system should have a dissociation constant in the "off" state that is greater (i.e., weaker affinity) than the concentrations of the molecules that are being controlled, and ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.6b00529

    authors： Zimmerman SP,Hallett RA,Bourke AM,Bear JE,Kennedy MJ,Kuhlman B

    更新日期：2016-09-20 00:00:00

  • Human 3'-phosphoadenosine 5'-phosphosulfate synthetase (isoform 1, brain): kinetic properties of the adenosine triphosphate sulfurylase and adenosine 5'-phosphosulfate kinase domains.

    abstract：:Recombinant human 3'-phosphoadenosine 5'-phosphosulfate (PAPS) synthetase, isoform 1 (brain), was purified to near-homogeneity from an Escherichia coli expression system and kinetically characterized. The native enzyme, a dimer with each 71 kDa subunit containing an adenosine triphosphate (ATP) sulfurylase and an aden...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi049827m

    authors： Lansdon EB,Fisher AJ,Segel IH

    更新日期：2004-04-13 00:00:00

  • Kinetic and Bioinformatic Characterization of d-2-Hydroxyglutarate Dehydrogenase from Pseudomonas aeruginosa PAO1.

    abstract：:d-2-Hydroxyglutarate dehydrogenase from Pseudomonas aeruginosa PAO1 (PaD2HGDH) catalyzes the oxidation of d-2-hydroxyglutarate to 2-ketoglutarate, which is a necessary step in the serine biosynthetic pathway. The dependence of P. aeruginosa on PaD2HGDH makes the enzyme a potential therapeutic target against P. aerugin...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.0c00832

    authors： Quaye JA,Gadda G

    更新日期：2020-12-29 00:00:00

  • Bacterial ApbC can bind and effectively transfer iron-sulfur clusters.

    abstract：:The metabolism of iron-sulfur ([Fe-S]) clusters requires a complex set of machinery that is still being defined. Mutants of Salmonella enterica lacking apbC have nutritional and biochemical properties indicative of defects in [Fe-S] cluster metabolism. ApbC is a 40.8 kDa homodimeric ATPase and as purified contains lit...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi800551y

    authors： Boyd JM,Pierik AJ,Netz DJ,Lill R,Downs DM

    更新日期：2008-08-05 00:00:00

  • The swapping of terminal arms in ribonucleases: comparison of the solution structure of monomeric bovine seminal and pancreatic ribonucleases.

    abstract：:Bovine seminal ribonuclease (BS-RNase), the only dimeric protein among the pancreatic-like ribonucleases, is endowed with special structural features and with biological functions beyond enzymatic activity. In solution, the protein exists as an equilibrium mixture of two forms, with or without exchange (or swapping) o...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0342517

    authors： Avitabile F,Alfano C,Spadaccini R,Crescenzi O,D'Ursi AM,D'Alessio G,Tancredi T,Picone D

    更新日期：2003-07-29 00:00:00

  • A conserved region in alpha-macroglobulins participates in binding to the mammalian alpha-macroglobulin receptor.

    abstract：:Efforts to characterize the receptor recognition domain of alpha-macroglobulins have primarily focused on human alpha 2-macroglobulin (alpha 2M). In the present work, the structure and function of the alpha-macroglobulin receptor recognition site were investigated by amino acid sequence analysis, plasma clearance, and...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00429a069

    authors： Enghild JJ,Thøgersen IB,Roche PA,Pizzo SV

    更新日期：1989-02-07 00:00:00

  • Identification of a regulatory segment of poly(ADP-ribose) glycohydrolase.

    abstract：:Coordinate regulation of PARP-1 and -2 and PARG is required for cellular responses to genotoxic stress. While PARP-1 and -2 are regulated by DNA breaks and covalent modifications, mechanisms of PARG regulation are poorly understood. We report here discovery of a PARG regulatory segment far removed linearly from residu...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi100973m

    authors： Botta D,Jacobson MK

    更新日期：2010-09-07 00:00:00

  • Mapping the suramin-binding sites of human neutrophil elastase: investigation by fluorescence resonance energy transfer and molecular modeling.

    abstract：:Neutrophil elastase (NE), a mediator of inflammation, binds with high affinity numerous anionic molecules including suramin, a polysulfated naphthylurea, which inhibits it with a Ki of 0.2 microM and a 4:1 suramin:NE stoichiometry and thus constitutes a potential therapeutic agent. In an attempt to locate the suramin ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi971029r

    authors： Mély Y,Cadène M,Sylte I,Bieth JG

    更新日期：1997-12-16 00:00:00

  • Amino group environments and metal binding properties of carbon-13 reductively methylated bovine alpha-lactalbumin.

    abstract：:13C NMR spectroscopy has been used to study the amino group environments and metal binding properties of 13C reductively methylated bovine alpha-lactalbumin. Bovine alpha-lactalbumin is a Ca2+ metalloprotein containing 12 lysyl amino groups and a free amino terminus. All 13 amino groups can be 13C-dimethylated without...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00315a026

    authors： Gerken TA

    更新日期：1984-09-25 00:00:00

  • Motility of dimeric ncd on a metal-chelating surfactant: evidence that ncd is not processive.

    abstract：:The surface immobilization methods that allowed single-molecule motility experiments with native kinesin have not worked with the ncd motor protein and other kinesin-related motors. To solve this problem, a surfactant (Pluronic F108) was chemically modified with the metal-chelating group nitrilotriacetic acid (NTA) to...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9829175

    authors： deCastro MJ,Ho CH,Stewart RJ

    更新日期：1999-04-20 00:00:00

  • Cadmium-113 nuclear magnetic resonance studies of cadmium-substituted derivatives of bovine superoxide dismutase.

    abstract：:We have prepared the following cadmium-113-substituted derivatives of bovine superoxide dismutase and recorded the nuclear magnetic resonance (NMR) spectrum of the cadmium: 2Cd(II), in which Cd(II) is presumed to bind to the Zn(II) site and the copper site is unoccupied, and 2Cd(II)--2Cu(I), which is analogous to the ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00544a031

    authors： Bailey DB,Ellis PD,Fee JA

    更新日期：1980-02-05 00:00:00

  • 1H NMR sequential resonance assignments, secondary structure, and global fold in solution of the major (trans-Pro43) form of bovine calbindin D9k.

    abstract：:A wide range of two-dimensional 1H NMR experiments have been used to completely assign the 500-MHz 1H NMR spectrum of recombinant Ca2+-saturated bovine calbindin D9k (76 amino acids, Mr = 8500). In solution, calbindin D9k exists as an equilibrium mixture of isoforms with trans (75%) and cis (25%) isomers of the peptid...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00443a043

    authors： Kördel J,Forsén S,Chazin WJ

    更新日期：1989-08-22 00:00:00

  • Multiple forms of phosphodeoxyribomutase from Escherichia coli. Physical and chemical characterization.

    abstract：:Phosphodeoxyribomutase from Escherichia coli has been purified to homogeneity. Chromatography on DEAE-Sephadex revealed three peaks of enzyme activity, designated form I, form II, and form III. Form III could be further separated into form III-1 and form III-2 by polyacrylamide gel electrophoresis. The four different ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00674a021

    authors： Leer JC,Hammer-Jespersen K

    更新日期：1975-02-11 00:00:00

  • A three-dimensional molecular model of lipid-free apolipoprotein A-I determined by cross-linking/mass spectrometry and sequence threading.

    abstract：:Apolipoprotein (apo) A-I, a 243-residue, 28.1-kDa protein is a major mediator of the reverse cholesterol transport (RCT) pathway, a process that may reduce the risk of cardiovascular disease in humans. In plasma, a small fraction of lipid-free or lipid-poor apoA-I is likely a key player in the first step of RCT. There...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi047717+

    authors： Silva RA,Hilliard GM,Fang J,Macha S,Davidson WS

    更新日期：2005-03-01 00:00:00

  • Membrane-associated c-type cytochromes from the green sulfur bacterium Chlorobium limicola forma thiosulfatophilum: purification and characterization of cytochrome c553.

    abstract：:Tetraheme cytochromes involved in photosynthetic electron transport have previously been described associated with the reaction centers of purple photosynthetic bacteria; however, similar heme proteins have not until now been characterized in the phylogenetically distinct green sulfur bacteria. In this paper we descri...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi962624g

    authors： Albouy D,Sturgis JN,Feiler U,Nitschke W,Robert B

    更新日期：1997-02-18 00:00:00

  • Phosphorylation of calmodulin alters its potency as an activator of target enzymes.

    abstract：:Previous work has shown that calmodulin (CaM) is constitutively phosphorylated in rat liver, probably by casein kinase II [Quadroni, M., James, P., and Carafoli, E. (1994) J. Biol. Chem. 269, 16116-16122]. A procedure is now described for the isolation of the phosphorylated forms of calmodulin (PCaM) free from CaM, si...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi972930+

    authors： Quadroni M,L'Hostis EL,Corti C,Myagkikh I,Durussel I,Cox J,James P,Carafoli E

    更新日期：1998-05-05 00:00:00

  • Cytochrome P-450 transfer from adrenocortical submitochondrial particles to liposome membranes.

    abstract：:The transfer of cytochrome P-450 from bovine adrenocortical submitochondrial particles (smp) to unilamellar liposome membranes was investigated using a table top ultracentrifuge. Submitochondrial particles were incubated with liposome membranes at 25 degrees C and precipitated by ultracentrifugation at 200000g for a f...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00014a041

    authors： Kominami S,Onizuka M,Tasaka-Marumoto C,Takemori S

    更新日期：1995-04-11 00:00:00

  • Hypoxia Inducible Factors Modulate Mitochondrial Oxygen Consumption and Transcriptional Regulation of Nuclear-Encoded Electron Transport Chain Genes.

    abstract：:Hypoxia inducible factor-1 (HIF1) is a stress-responsive nuclear transcription factor that is activated with a decrease in oxygen availability. HIF1 regulates the expression of genes involved in a cell's adaptation to hypoxic stress, including those with mitochondrial specific function. To gain a more comprehensive un...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi5012892

    authors： Hwang HJ,Lynn SG,Vengellur A,Saini Y,Grier EA,Ferguson-Miller SM,LaPres JJ

    更新日期：2015-06-23 00:00:00

  • Temperature-dependent CD and NMR studies on a synthetic oligonucleotide containing a B-Z junction at high salt.

    abstract：:It is now accepted that two or more conformations may exist within the same DNA molecule, thereby generating conformational junctions. The presence of B-Z junctions between right- and left-handed DNA conformations has been detected in plasmids by a number of techniques. Preliminary characterization of the first exampl...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00428a046

    authors： Sheardy RD,Winkle SA

    更新日期：1989-01-24 00:00:00

  • Mechanistic studies of aromatic amine dehydrogenase, a tryptophan tryptophylquinone enzyme.

    abstract：:Aromatic amine dehydrogenase (AADH) is the second enzyme known to possess the tryptophan tryptophylquinone (TTQ) prosthetic group. Its ability to catalyze the oxidative deamination of a wide range of aromatic and aliphatic amines has been investigated. Steady-state and transient kinetic studies of the reaction of AADH...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00003a015

    authors： Hyun YL,Davidson VL

    更新日期：1995-01-24 00:00:00

  • Interaction of the low-affinity receptor CD23/Fc epsilonRII lectin domain with the Fc epsilon3-4 fragment of human immunoglobulin E.

    abstract：:CD23/Fc epsilonRII, the low-affinity receptor for IgE, is a multifunctional protein of importance in blood cell development and the immune system. We have studied the interaction of CD23 with IgE in solution using hydrodynamic methods applied to recombinant fragments of both ligands: sCD23, corresponding to the solubl...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi961231e

    authors： Shi J,Ghirlando R,Beavil RL,Beavil AJ,Keown MB,Young RJ,Owens RJ,Sutton BJ,Gould HJ

    更新日期：1997-02-25 00:00:00