Sum of the parts: mass spectrometry-based metabolomics.


:Metabolomics is a rapidly growing field of research used in the identification and quantification of the small molecule metabolites within an organism, thereby providing insights into cell metabolism and bioenergetics as well as processes important in clinical medicine, such as disposition of pharmaceutical compounds. It offers comprehensive information about thousands of low-molecular mass compounds (<1500 Da) that represent a wide range of pathways and intermediary metabolism. Because of its vast expansion in the past two decades, mass spectrometry has become an indispensable tool in "omic" analyses. The use of different ionization techniques such as the more traditional electrospray and matrix-assisted laser desorption, as well as recently popular desorption electrospray ionization, has allowed the analysis of a wide range of biomolecules (e.g., peptides, proteins, lipids, and sugars), and their imaging and analysis in the original sample environment in a workup free fashion. An overview of the current state of the methodology is given, as well as examples of application.






Milne SB,Mathews TP,Myers DS,Ivanova PT,Brown HA




Has Abstract


2013-06-04 00:00:00












  • Oligonucleotide-directed switching of DNA polymerases to a dead-end track.

    abstract::During DNA replication, the presence of oligonucleotides with partial homology to the template strand was shown to induce a switch of the polymerase from the normal template to the oligonucleotide. The latter acted as a dead-end template and led to abortive replication. The only prerequisite was that the oligonucleoti...


    pub_type: 杂志文章


    authors: Guieysse AL,Praseuth D,Grigoriev M,Harel-Bellan A,Hélène C

    更新日期:1995-07-18 00:00:00

  • Resonance Raman evidence for the presence of two heme pocket conformations with varied activities in CO-bound bovine soluble guanylate cyclase and their conversion.

    abstract::Resonance Raman (RR) spectra of soluble guanylate cyclase (sGC) reported by five independent research groups have been classified as two types: sGC(1) and sGC(2). Here we demonstrate that the RR spectra of sGC isolated from bovine lung contain only sGC(2) while both species are observed in the spectra of the CO-bound ...


    pub_type: 杂志文章


    authors: Li Z,Pal B,Takenaka S,Tsuyama S,Kitagawa T

    更新日期:2005-01-25 00:00:00

  • Differential scanning calorimetry and CD spectrometry of acclimation temperature-associated types of carp light meromyosin.

    abstract::Differential scanning calorimetry and CD spectrometry were employed to study the thermal unfolding of light meromyosin (LMM) prepared from carp acclimated to different temperatures. The transition temperatures given by the major peaks at pH 8.0 in 0.6 M KCl for LMM from carp acclimated to 10 degrees C were 32.5 and 39...


    pub_type: 杂志文章


    authors: Nakaya M,Kakinuma M,Watabe S,Ooi T

    更新日期:1997-07-29 00:00:00

  • Chaotropic agents and increased matrix volume enhance binding of mitochondrial cyclophilin to the inner mitochondrial membrane and sensitize the mitochondrial permeability transition to [Ca2+].

    abstract::Binding of mitochondrial cyclophilin (CyP) to the inner mitochondrial membrane is induced by treatment of mitochondria with thiol reagents or oxidative stress and correlates with a sensitization to [Ca2+] of the cyclosporin A-sensitive mitochondrial permeability transition pore (MTP) [Connern, C. P., & Halestrap, A. P...


    pub_type: 杂志文章


    authors: Connern CP,Halestrap AP

    更新日期:1996-06-25 00:00:00

  • The role of serine 167 in human indoleamine 2,3-dioxygenase: a comparison with tryptophan 2,3-dioxygenase.

    abstract::The initial step in the l-kynurenine pathway is oxidation of l-tryptophan to N-formylkynurenine and is catalyzed by one of two heme enzymes, tryptophan 2,3-dioxygenase (TDO) or indoleamine 2,3-dioxygenase (IDO). Here, we address the role of the conserved active site Ser167 residue in human IDO (S167A and S167H variant...


    pub_type: 杂志文章


    authors: Chauhan N,Basran J,Efimov I,Svistunenko DA,Seward HE,Moody PC,Raven EL

    更新日期:2008-04-22 00:00:00

  • The 68 kDa calmodulin-binding protein is tightly associated with the multiprotein DNA polymerase alpha-primase complex in HeLa cells.

    abstract::Calcium and its receptor protein calmodulin function in the regulation of proliferation of mammalian cells. A 68 kDa calmodulin-specific binding protein was shown previously to be associated with growth factor-dependent progression of a variety of mammalian cells from G1 to S phase and to stimulate DNA synthesis in pe...


    pub_type: 杂志文章


    authors: Cao QP,McGrath CA,Baril EF,Quesenberry PJ,Reddy GP

    更新日期:1995-03-28 00:00:00

  • Single-molecule studies of bacterial protein translocation.

    abstract::In prokaryotes, a large share of the proteins are secreted from the cell through a process that requires their translocation across the cytoplasmic membrane. This process is mediated by the universally conserved Sec system with homologues in the endoplasmic reticulum and thylakoid membranes of eukaryotes. The Sec syst...


    pub_type: 杂志文章,评审


    authors: Kedrov A,Kusters I,Driessen AJ

    更新日期:2013-10-01 00:00:00

  • Conformational states of N-acylalanine dithio esters: correlation of resonance Raman spectra with structures.

    abstract::The conformational states of N-acylalanine dithio esters, involving rotational isomers about the RC(=O)NH--CH(CH3) and NHCH(CH3)--C(=S) bonds, are defined and compared to those of N-acylglycine dithio esters. The structure of N-(p-nitrobenzoyl)-DL-alanine ethyl dithio ester has been determined by X-ray crystallographi...


    pub_type: 杂志文章


    authors: Lee H,Angus RH,Storer AC,Varughese KI,Carey PR

    更新日期:1988-01-12 00:00:00

  • Mechanism of interactions of alpha-naphthoflavone with cytochrome P450 3A4 explored with an engineered enzyme bearing a fluorescent probe.

    abstract::Design of a partially cysteine-depleted C98S/C239S/C377S/C468A cytochrome P450 3A4 mutant designated CYP3A4(C58,C64) allowed site-directed incorporation of thiol-reactive fluorescent probes into alpha-helix A. The site of modification was identified as Cys-64 with the help of CYP3A4(C58) and CYP3A4(C64), each bearing ...


    pub_type: 杂志文章


    authors: Tsalkova TN,Davydova NY,Halpert JR,Davydov DR

    更新日期:2007-01-09 00:00:00

  • In vitro phosphinate methylation by PhpK from Kitasatospora phosalacinea.

    abstract::Radical S-adenosyl-L-methionine, cobalamin-dependent methyltransferases have been proposed to catalyze the methylations of unreactive carbon or phosphorus atoms in antibiotic biosynthetic pathways. To date, none of these enzymes has been purified or shown to be active in vitro. Here we demonstrate the activity of the ...


    pub_type: 杂志文章


    authors: Werner WJ,Allen KD,Hu K,Helms GL,Chen BS,Wang SC

    更新日期:2011-10-25 00:00:00

  • Domain characteristics of the carboxyl-terminal fragment 206-316 of thermolysin: immunochemical studies.

    abstract::The extent of nativeness of the stable conformation of the thermolysin fragment containing the carboxyl-terminal third of the protein (from residues 206 to 316, denoted fragment FII) was examined by its immunogenic and antigenic characteristics. Antisera elicited in rabbits by either intact thermolysin or fragment FII...


    pub_type: 杂志文章


    authors: Vita C,Fontana A,Chaiken IM

    更新日期:1982-04-27 00:00:00

  • Novel Cl(-)-dependent intracellular pH regulation in murine MDR 1 transfectants and potential implications.

    abstract::Previously [Luz et al. (1994) Biochemistry 33, 7239-7249], we determined that Cl(-)- and -HCO3-dependent pHi homeostasis was perturbed in multidrug resistant (MDR) cells created by transfecting LR73 Chinese hamster ovary fibroblasts with wild-type mu (murine) MDR 1 (Gros et al., 1991). Via single-cell photometry exper...


    pub_type: 杂志文章


    authors: Roepe PD,Weisburg JH,Luz JG,Hoffman MM,Wei LY

    更新日期:1994-09-13 00:00:00

  • Thermodynamics of glycophorin in phospholipid bilayer membranes.

    abstract::We have developed a model of glycophorin in a phospholipid bilayer membrane in order to study the thermodynamics of this system and to understand the detailed behavior of recent calorimetric data. We assume that the larger glycophorin polar group can be considered as either adopting a pancakelike conformation at the b...


    pub_type: 杂志文章


    authors: MacDonald AL,Pink DA

    更新日期:1987-04-07 00:00:00

  • Synthesis of (2R,3R)-erythro- and (2R,3S)-threo-fluoromalate using malic dehydrogenase; stereoselectivity of malic dehydrogenase.

    abstract::3-Fluorooxalacetate is a substrate for malic dehydrogenase. When enzymatic reduction is slower than the rate of epimerization of the two enantiomers, only (2R,3R)-erythro-fluoromalate is formed. Conversely, when a high enzyme level and excess of NADH lead to reduction that is fast relative to the epimerization rate, e...


    pub_type: 杂志文章


    authors: Urbauer JL,Bradshaw DE,Cleland WW

    更新日期:1998-12-22 00:00:00

  • Resolution of oligomeric species during the aggregation of Aβ1-40 using (19)F NMR.

    abstract::In the commonly used nucleation-dependent model of protein aggregation, aggregation proceeds only after a lag phase in which the concentration of energetically unfavorable nuclei reaches a critical value. The formation of oligomeric species prior to aggregation can be difficult to detect by current spectroscopic techn...


    pub_type: 杂志文章


    authors: Suzuki Y,Brender JR,Soper MT,Krishnamoorthy J,Zhou Y,Ruotolo BT,Kotov NA,Ramamoorthy A,Marsh EN

    更新日期:2013-03-19 00:00:00

  • High-efficiency triple-helix-mediated photo-cross-linking at a targeted site within a selectable mammalian gene.

    abstract::Targeting damage to specific sites in the genome represents an attractive approach to manipulating gene function in mammalian cells. To test the applicability of triple-helix formation as a means for achieving precisely timed site-specific damage within a mammalian gene, a triplex-forming oligodeoxyribonucleotide (TFO...


    pub_type: 杂志文章


    authors: Vasquez KM,Wensel TG,Hogan ME,Wilson JH

    更新日期:1996-08-20 00:00:00

  • Binding of cis- and trans-diamminedichloroplatinum(II) to deoxyribonucleic acid exposes nucleosides as measured immunochemically with anti-nucleoside antibodies.

    abstract::We report the use of anti-nucleoside antibodies to probe for local denaturation of calf thymus DNA upon binding of the antitumor drug cis-diamminedichloroplatinum(II), cis-DDP, and the biologically inactive analogues trans-diamminedichloroplatinum(II), trans-DDP, and chloro(diethylenetriamine)platinum(II) chloride, [P...


    pub_type: 杂志文章


    authors: Sundquist WI,Lippard SJ,Stollar BD

    更新日期:1986-04-08 00:00:00

  • Proton and deuteron nuclear magnetic relaxation dispersion studies of Ca2+-Mn2+-lentil lectin and Ca2+-Mn2+-pea lectin: evidence for a site of solvent exchange in common with concanavalin A.

    abstract::Measurements of the magnetic field dependence of the longitudinal magnetic relaxation rates (NMRD profiles) of solvent protons and deuterons led to the discovery of two classes of solvent binding sites in Ca2+-Mn2+-concanavalin A (CMPL) [Koenig, S. H., Brown, R. D., III, & Brewer, C. F. (1985) Biochemistry (second of ...


    pub_type: 杂志文章


    authors: Bhattacharyya L,Brewer CF,Brown RD 3rd,Koenig SH

    更新日期:1985-09-10 00:00:00

  • The N-terminal fragment of human parathyroid hormone receptor 1 constitutes a hormone binding domain and reveals a distinct disulfide pattern.

    abstract::The N-terminal extracellular parts of human G-protein coupled receptor class B, for example, receptors for secretin, glucagon, or parathyroid hormone, are involved in ligand binding. To obtain structural and functional information on the N-terminal receptor fragment of human parathyroid hormone receptor 1 (PTHR1), the...


    pub_type: 杂志文章


    authors: Grauschopf U,Lilie H,Honold K,Wozny M,Reusch D,Esswein A,Schäfer W,Rücknagel KP,Rudolph R

    更新日期:2000-08-01 00:00:00

  • Relaxation kinetics of glutamate dehydrogenase self-association by pressure perturbation.

    abstract::The kinetics of self-association for beef liver glutamate dehydrogenase (EC have been measured by using pressure perturbation in both the time domain and the frequency domain by monitoring scattered light intensity. The kinetic behavior is entirely consistent with the random self-association model proposed by...


    pub_type: 杂志文章


    authors: Halvorson HR

    更新日期:1979-06-12 00:00:00

  • Relative susceptibilities of the glucosamine-glucuronic acid and N-acetylglucosamine-glucuronic acid linkages to heparin lyase III.

    abstract::Heparin lyases are valuable tools for generating oligosaccharide fragments and in sequence determination of heparan sulfate (HS). Heparin lyase III is known to cleave the linkages between N-acetylglucosamine (GlcNAc) or N-sulfated glucosamine (GlcNS) and glucuronic acid (GlcA) as the primary sites and the linkages bet...


    pub_type: 杂志文章


    authors: Chai W,Leteux C,Westling C,Lindahl U,Feizi T

    更新日期:2004-07-06 00:00:00

  • Evidence for a quantitative tissue-specific distribution of high mobility group chromosomal proteins.

    abstract::The quantitative tissue specificity of the high mobility group (HMG) chromosomal proteins was investigated. Perchloric acid (PCA) extracts of four different chicken tissues and erythrocytes contained three proteins which comigrated on NaDodSO4-polyacrylamide gels with the HMG's 1,2, and E from erythrocyte nuclei. Thes...


    pub_type: 杂志文章


    authors: Gordon JS,Rosenfeld BI,Kaufman R,Williams DL

    更新日期:1980-09-16 00:00:00

  • Constitutive activation of opsin: interaction of mutants with rhodopsin kinase and arrestin.

    abstract::Mutation of Gly90, Glu113, Ala292, and Lys296 in the visual pigment rhodopsin constitutively activates the protein for activation of the G protein transducin. Three of these mutations have been shown to cause two different human diseases. Mutation of Gly90 and Ala292 results in complete night blindness, and mutation o...


    pub_type: 杂志文章


    authors: Rim J,Oprian DD

    更新日期:1995-09-19 00:00:00

  • 1H NMR studies of lambda cro repressor. 2. Sequential resonance assignments of the 1H NMR spectrum.

    abstract::The cro repressor protein from bacteriophage lambda has been studied in solution by two-dimensional nuclear magnetic resonance spectroscopy (2D NMR). Following the approach of Wüthrich and co-workers [Wüthrich, K., Wider, G., Wagner, G., & Braun, W. (1982) J. Mol. Biol. 155, 311-319], individual spin systems were iden...


    pub_type: 杂志文章


    authors: Weber PL,Wemmer DE,Reid BR

    更新日期:1985-08-13 00:00:00

  • Chemical Interactions of Polyethylene Glycols (PEGs) and Glycerol with Protein Functional Groups: Applications to Effects of PEG and Glycerol on Protein Processes.

    abstract::In this work, we obtain the data needed to predict chemical interactions of polyethylene glycols (PEGs) and glycerol with proteins and related organic compounds and thereby interpret or predict chemical effects of PEGs on protein processes. To accomplish this, we determine interactions of glycerol and tetraEG with >30...


    pub_type: 杂志文章


    authors: Knowles DB,Shkel IA,Phan NM,Sternke M,Lingeman E,Cheng X,Cheng L,O'Connor K,Record MT

    更新日期:2015-06-09 00:00:00

  • Deglycosylated human follitropin: characterization and effects on adenosine cyclic 3',5'-phosphate production in porcine granulosa cells.

    abstract::Chemical deglycosylation of gonadotropins with hydrogen fluoride (HF) has facilitated the investigation of the structure-function relationship of the individual peptide and oligosaccharide moieties in the mechanism of hormone action. These studies have dealt almost exclusively with lutropin or human choriogonadotropin...


    pub_type: 杂志文章


    authors: Calvo FO,Keutmann HT,Bergert ER,Ryan RJ

    更新日期:1986-07-01 00:00:00

  • Mandelate racemase from Pseudomonas putida. Magnetic resonance and kinetic studies of the mechanism of catalysis.

    abstract::The interactions of mandelate racemase with divalent metal ion, substrate, and competitive inhibitors were investigated. The enzyme was found by electron paramagnetic resonance (EPR) to bind 0.9 Mn2+ ion per subunit with a dissociation constant of 8 muM, in agreement with its kinetically determined activator constant....


    pub_type: 杂志文章


    authors: Maggio ET,Kenyon GL,Mildvan AS,Hegeman GD

    更新日期:1975-03-25 00:00:00

  • Unique binding of a novel synthetic inhibitor, N-[3-[4-[4-(amidinophenoxy)carbonyl]phenyl]-2-methyl-2-propenoyl]- N-allylglycine methanesulfonate, to bovine trypsin, revealed by the crystal structure of the complex.

    abstract::Trypsin and N-[3-[4-[4-(amidinophenoxy)carbonyl]phenyl]-2-methyl-2-propenoyl]- N-allylglycine methanesulfonate (1), a newly designed and orally active synthetic trypsin inhibitor, were cocrystallized. The space group of the crystal is P2(1)2(1)2(1) with cell constants a = 63.74 A, b = 63.08 A, and c = 69.38 A, which i...


    pub_type: 杂志文章


    authors: Odagaki Y,Nakai H,Senokuchi K,Kawamura M,Hamanaka N,Nakamura M,Tomoo K,Ishida T

    更新日期:1995-10-03 00:00:00

  • Chromatographic behavior of cyclic AMP dependent protein kinase and its subunits from Dictyostelium discoideum.

    abstract::An adenosine cyclic 3',5'-monophosphate (cAMP) dependent protein kinase has recently been shown to exist in Dictyostelium discoideum and to be developmentally regulated. In this report we have followed the chromatographic behavior of both the holoenzyme and its subunits. A cAMP-dependent holoenzyme could be obtained f...


    pub_type: 杂志文章


    authors: Rutherford CL,Vaughan RL,Cloutier MJ,Ferris DK,Brickey DA

    更新日期:1984-09-25 00:00:00

  • Contrasting synergistic anion effects in vanadium(V) binding to nicatransferrin versus human serum transferrin.

    abstract::Some ascidians sequester vanadium and other metal ions that are bound and transported in higher organisms by transferrin. The ascidian Ciona intestinalis has a monolobal transferrin (nicatransferrin) in its plasma. The binding of vanadium(V) to nicatransferrin was investigated by using isothermal titration calorimetry...


    pub_type: 杂志文章


    authors: Gaffney JP,Valentine AM

    更新日期:2009-12-15 00:00:00