Abstract：
:Metabolomics is a rapidly growing field of research used in the identification and quantification of the small molecule metabolites within an organism, thereby providing insights into cell metabolism and bioenergetics as well as processes important in clinical medicine, such as disposition of pharmaceutical compounds. It offers comprehensive information about thousands of low-molecular mass compounds (<1500 Da) that represent a wide range of pathways and intermediary metabolism. Because of its vast expansion in the past two decades, mass spectrometry has become an indispensable tool in "omic" analyses. The use of different ionization techniques such as the more traditional electrospray and matrix-assisted laser desorption, as well as recently popular desorption electrospray ionization, has allowed the analysis of a wide range of biomolecules (e.g., peptides, proteins, lipids, and sugars), and their imaging and analysis in the original sample environment in a workup free fashion. An overview of the current state of the methodology is given, as well as examples of application.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Milne SB,Mathews TP,Myers DS,Ivanova PT,Brown HAdoi
10.1021/bi400060esubject
Has Abstractpub_date
2013-06-04 00:00:00pages
3829-40issue
22eissn
0006-2960issn
1520-4995journal_volume
52pub_type
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