Stabilities of nucleotide loops bridging the pyrimidine strands in DNA pyrimidine.purine.pyrimidine triplexes: special stability of the CTTTG loop.

abstract：:The Ca2+-dependent regulation of smooth muscle actomyosin involves a myosin light chain kinase (ATP: myosin light chain phosphotransferase). It has been shown (Dabrowska, R., Aromatorio, D., Sherry, J.M.F., and Hartshorne, D.J. 1977, Biochem. Biophys. Res. Commun. 78, 1263) that the kinase is composed of two proteins ...

journal_title：Biochemistry

authors： Dabrowska R,Sherry JM,Aromatorio DK,Hartshorne DJ

更新日期：1978-01-24 00:00:00

abstract：:The thermal dependence of the structural stability of membrane-reconstituted yeast cytochrome c oxidase has been studied by using different techniques including high-sensitivity differential scanning calorimetry, differential detergent solubility thermal gel analysis, and enzyme activity measurements. For these studie...

journal_title：Biochemistry

authors： Morin PE,Diggs D,Freire E

更新日期：1990-01-23 00:00:00

abstract：:Monoamine oxidase B (MAO B) is an integral protein of the outer mitochondrial membrane that is involved in the deamination of vasoactive and neuroactive amines. The oxidation of these amine substrates requires the cofactor FAD, which is covalently bound to Cys-397 of human MAO B. Previously, Glu-34 and Tyr-44 of MAO B...

journal_title：Biochemistry

authors： Kirksey TJ,Kwan SW,Abell CW

更新日期：1998-09-01 00:00:00

abstract：:The cooperative binding of the Escherichia coli DNA unwinding protein to single-stranded DNA has been studied by electron microscopy. Analysis of the electron microscopic data by means of a simple statistical mechanical model has yielded a value of 3.8-7.6 X 10(10) l./mol for the cooperative binding constant in 0.15 M...

journal_title：Biochemistry

authors： Ruyechan WT,Wetmur JG

更新日期：1975-12-16 00:00:00

abstract：:Runx proteins constitute a family of mammalian transcription factors that interact with DNA through their evolutionarily conserved Runt domain. CBFbeta, alternatively denoted PEBP2beta, is the non-DNA-binding heterodimer partner and acts to enhance the DNA binding affinity of Runx proteins. Runx proteins and CBFbeta a...

journal_title：Biochemistry

authors： Wolf-Watz M,Grundström T,Härd T

更新日期：2001-09-25 00:00:00

abstract：:Lactone-hydrolyzing enzymes derived from some Bacillus species are capable of disrupting quorum sensing in bacteria that use N-acyl-l-homoserine lactones (AHLs) as intercellular signaling molecules. Despite the promise of these quorum-quenching enzymes as therapeutic and anti-biofouling agents, the ring opening mechan...

journal_title：Biochemistry

authors： Momb J,Thomas PW,Breece RM,Tierney DL,Fast W

更新日期：2006-11-07 00:00:00

abstract：:A chromatin-bound protease, active in 2 M NaCl-5 M urea or 5 M urea alone, was demonstrated in rat liver, kidney, testes, brain, rabbit bone marrow, chicken reticulocyte, and Ehrlich ascites chromatin. Chicken erythrocyte chromatin did not possess any detectable proteolytic activity in salt and urea. The proteolytic a...

journal_title：Biochemistry

authors： Carter DB,Chae CB

更新日期：1976-01-13 00:00:00

abstract：:The inhibitory effect of phosphorothioate residues, located within one strand of double-stranded DNA, on the hydrolytic activity of the restriction endonuclease EcoRV was investigated. Specific incorporation of a phosphorothioate group at the site of cleavage yielded the sequence 5'-GATsATC-3'. This modified sequence ...

journal_title：Biochemistry

authors： Olsen DB,Kotzorek G,Eckstein F

更新日期：1990-10-16 00:00:00

abstract：:In the present report we studied the interaction between the skeletal muscle ryanodine receptor and the ubiquitous S100A1 Ca2+ binding protein. S100A1 did not affect equilibrium [3H]ryanodine binding to purified rabbit skeletal muscle terminal cisternae at 100 microM free [Ca2+]. At nanomolar free [Ca2+], however, S10...

journal_title：Biochemistry

authors： Treves S,Scutari E,Robert M,Groh S,Ottolia M,Prestipino G,Ronjat M,Zorzato F

更新日期：1997-09-23 00:00:00

abstract：:A likely entry/exit path for nitrogenase substrates, products, and/or protons involves residues α277(Arg), α192(Ser), and α356(Gly), all of which are highly conserved among MoFe proteins from different organisms. The α192(Ser) and α277(Arg) residues form part of a hydrogen-bonded network that also involves α195(His), ...

journal_title：Biochemistry

authors： Fisher K,Hare ND,Newton WE

更新日期：2014-10-07 00:00:00

abstract：:Aristolochene synthase, a metal-dependent sesquiterpene cyclase from Aspergillus terreus, catalyzes the ionization-dependent cyclization of farnesyl diphosphate (FPP) to form the bicyclic eremophilane (+)-aristolochene with perfect structural and stereochemical precision. Here, we report the X-ray crystal structure of...

journal_title：Biochemistry

authors： Chen M,Al-lami N,Janvier M,D'Antonio EL,Faraldos JA,Cane DE,Allemann RK,Christianson DW

更新日期：2013-08-13 00:00:00

abstract：:Sequence effects on the stability of purine-motif (also called (G, A)-motif) triple helix have been investigated through two symmetry-related systems: one of them had a 5'(GGA)43' core sequence of triplex-forming oligonucleotides (TFOs), whereas the other one had a reversed 5'(AGG)43' core sequence. These (G,A)-contai...

journal_title：Biochemistry

authors： Arimondo PB,Barcelo F,Sun JS,Maurizot JC,Garestier T,Hélène C

更新日期：1998-11-24 00:00:00

abstract：:Isothiouronium compounds [Hoving, S., Bar-Shimon, M., Tijmes, J. J., Goldshleger, R., Tal, D. M., and Karlish, S. J. (1995) J. Biol. Chem. 270, 29788-29793] act as high-affinity competitive antagonists for Na(+) and K(+) (Rb(+)) on the renal Na(+)/K(+)-ATPase where they favor the E1 conformation. We have now character...

journal_title：Biochemistry

authors： Berman MC,Karlish SJ

更新日期：2003-04-01 00:00:00

abstract：:Voltage-dependent anion channel 1 (VDAC1) situated in the outer mitochondrial membrane regulates the transfer of various metabolites and is a key player in mitochondria-mediated apoptosis. Although many small chemicals that modulate the functions of VDAC1 have been reported to date, most, if not all, of them cannot be...

journal_title：Biochemistry

authors： Unten Y,Murai M,Yamamoto T,Watanabe A,Ichimaru N,Aburaya S,Aoki W,Shinohara Y,Miyoshi H

更新日期：2019-02-26 00:00:00

abstract：:Murine cDNA that encodes neuromodulin, a neurospecific calmodulin binding protein, was inserted into the plasmid pKK223-3 for expression in Escherichia coli. After being transformed into E. coli strain SG20252 (lon-), the expression vector directed the synthesis of a protein that was recognized by polyclonal antibodie...

journal_title：Biochemistry

authors： Au DC,Apel ED,Chapman ER,Estep RP,Nicolson TA,Storm DR

更新日期：1989-10-03 00:00:00

abstract：:DNA sequences with repetitive G-rich structural motifs, which form special structures called G-quadruplexes, widely exist in the human genome. Here we report the general peroxidase activity of G-quadruplex-hemin complexes and discuss the connection between peroxidase activity and G-quadruplex structures. The high pero...

journal_title：Biochemistry

authors： Cheng X,Liu X,Bing T,Cao Z,Shangguan D

更新日期：2009-08-25 00:00:00

abstract：:The antibiotic bicyclomycin inhibits rho-dependent termination processes by interfering with RNA translocation by preventing RNA binding at the translocation site or by uncoupling the translocation process from ATP hydrolysis. Previous studies have shown that bicyclomycin binds near the ATP hydrolysis pocket on rho. T...

journal_title：Biochemistry

authors： Vincent F,Openshaw M,Trautwein M,Gaskell SJ,Kohn H,Widger WR

更新日期：2000-08-08 00:00:00

abstract：:The distance between the calcium site (site I) and the zinc site (site II) in alpha-lactalbumin was estimated from Forster energy-transfer measurements between donor Eu(III) [or Tb(III)] at site I and acceptor Co(II) at site II to be 11.5 +/- 1.5 A. Intersite distances were also measured between the bis-ANS [4,4'-bis[...

journal_title：Biochemistry

authors： Musci G,Berliner LJ

更新日期：1986-08-26 00:00:00

abstract：:The binding of 125I-labeled ovine follitropin (oFSH) and 125I-labeled deglycosylated ovine follitropin (DG-oFSH) to porcine granulosa cell membranes was studied at equilibrium and nonequilibrium binding conditions and statistically analyzed. Saturation and competition binding experiments revealed homogeneity in the po...

journal_title：Biochemistry

authors： Sebok K,De Lean A,Sairam MR

更新日期：1987-06-16 00:00:00

abstract：:Fibronectin is composed of multiple homologous repeats and contains many functional domains. Two major heparin-binding domains have previously been identified: the Hep I site near the amino terminus and the Hep II site near the carboxyl terminus. The Hep II site has been considered the high-affinity heparin-binding si...

journal_title：Biochemistry

authors： Lin H,Lal R,Clegg DO

更新日期：2000-03-28 00:00:00

abstract：:The effects of pressure, up to 5 kbar, on multilamellar vesicles of 1,2-dipalmitoyl-sn-phosphatidylcholine perdeuterated in the acyl chains (DPPC-d62) were examined by using high-pressure NMR techniques. A deuterium probe was built, and the quadrupole splitting was measured against pressure at various temperatures. Th...

journal_title：Biochemistry

authors： Driscoll DA,Samarasinghe S,Adamy S,Jonas J,Jonas A

更新日期：1991-04-02 00:00:00

abstract：:The folding of the enzyme indole-3-glycerol-phosphate synthase (IGPS), a member of the (alpha/beta)8 fold family, has been studied. At least two folding intermediates have been detected using spectroscopic and activity measurements in combination with gel filtration chromatography. These two intermediates are produced...

journal_title：Biochemistry

authors： Sánchez del Pino MM,Fersht AR

更新日期：1997-05-06 00:00:00

abstract：:Several bifunctional alkylating agents of the aziridinylbenzoquinone class have been evaluated as potential antitumor agents. 3,6-Bis[(2-hydroxyethyl)amino]-2,5- diaziridinyl-1,4-benzoquinone (BZQ), 2,5-diaziridinyl-1,4-benzoquinone (DZQ), 3,6-bis(carboxyamino)-2,5-diaziridinyl- 1,4-benzoquinone (AZQ), and six analogu...

journal_title：Biochemistry

authors： Hartley JA,Berardini M,Ponti M,Gibson NW,Thompson AS,Thurston DE,Hoey BM,Butler J

更新日期：1991-12-17 00:00:00

abstract：:Melittin is a 26-residue peptide which undergoes high-affinity calcium-dependent binding by calmodulin [Barnette, M.S., Daly, R., & Weiss, B. (1983) Biochem. Pharmacol. 32, 2929; Comte, M., Maulet, Y., & Cox, J.A. (1983) Biochem. J. 209, 269; Anderson, S.R., & Malencik, D.A. (1986) Calcium Cell Funct. 6, 1]. The resul...

journal_title：Biochemistry

authors： Malencik DA,Anderson SR

更新日期：1988-03-22 00:00:00

abstract：:The interaction between the calcium binding and inhibitory components of troponin is central to the regulation of muscle contraction. In this work, two-dimensional heteronuclear single-quantum coherence nuclear magnetic resonance (2D-¿1H,15N¿-HSQC NMR) spectroscopy was used to determine the stoichiometry, affinity, an...

journal_title：Biochemistry

authors： Mercier P,Li MX,Sykes BD

更新日期：2000-03-21 00:00:00

abstract：:The kinetics of activation of Glu-plasminogen (Glu-Pg) and Lys77-Pg by two-chain recombinant tissue plasminogen activator (t-PA) were determined in the presence of isolated protein components of the extracellular matrix (ECM) and compared to activation in the presence of fibrinogen and fibrinogen fragments and in the ...

journal_title：Biochemistry

authors： Stack S,Gonzalez-Gronow M,Pizzo SV

更新日期：1990-05-22 00:00:00

abstract：:Previous studies have established that the mechanism of species-specific sponge cell reaggregation in vitro involves at least three components: a species-specific aggregation factor, its cell surface receptors, and Ca2+ ions. Here we report about the binding of Microciona 125I-labeled aggregation factor to cells and t...

journal_title：Biochemistry

authors： Jumblatt JE,Schlup V,Burger MM

更新日期：1980-03-04 00:00:00

abstract：:Histone H3 arginine 2 (H3R2) is post-translationally modified in three different states by "writers" of the protein arginine methyltransferase (PRMT) family. H3R2 methylarginine isoforms include PRMT5-catalyzed monomethylation (me1) and symmetric dimethylation (me2s) and PRMT6-catalyzed me1 and asymmetric dimethylatio...

journal_title：Biochemistry

authors： Lorton BM,Harijan RK,Burgos ES,Bonanno JB,Almo SC,Shechter D

更新日期：2020-10-06 00:00:00

abstract：:We have measured the base-pair lifetimes in oligodeoxynucleotides containing tracts of A.T base pairs using imino proton magnetic resonance. When the tract contains more than four consecutive A.T base pairs, possibly including a 5'-AT step but not a 5'-TA step, anomalously long lifetimes are observed. For example, the...

journal_title：Biochemistry

authors： Leroy JL,Charretier E,Kochoyan M,Guéron M

更新日期：1988-12-13 00:00:00

abstract：:The structural and motional properties of membrane lipids in various physical states and macroscopic organizations are elucidated by 1H NMR dipolar echo decay spectroscopy (DECODE). Multilamellar lipid dispersions in the gel (L beta') and liquid-crystalline (L alpha) states and a nonbilayer, hexagonal (HII) phase are ...

journal_title：Biochemistry

authors： Janes N,Rubin E,Taraschi TF

更新日期：1990-09-11 00:00:00