当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Quantitative Study of the Oligomerization of Yeast Prion Sup35NM Proteins.

Quantitative Study of the Oligomerization of Yeast Prion Sup35NM Proteins.

Abstract：

:The fibrillation of misfolded neurodegenerative disease-related proteins has been extensively studied over the past few decades, but the initial oligomerization has been rarely examined even though some recent evidence indicated that small protein oligomers are more neurotoxic than long protein fibers. It is rather difficult to study the initial oligomerization (nucleation) because most experimental methods, such as the β-sheet-related fluorescence assay and X-ray diffraction, are unable to detect small structureless protein oligomers. In this study, we have successfully developed a method to link a short thermally sensitive poly(N-isopropylacrylamide) (PNIPAM) chain to a model protein, Sup35NM, at a specific 31st residue site (Sup35NM-31m-PNIPAM) via the efficient thiol-ene Michael addition reaction. The oligomerization was studied by a combination of laser light scattering, the thioflavin T assay, and transmission electron microscopy. We found that the lag phase of Sup35NM was delayed from 12 to >24 h under the physiological condition after the PNIPAM linkage. The oligomerization and fibrillation constants decreased from 5.0 × 10-3 to 1.5 × 10-3 h-1 and from 3.0 × 10-2 to 1.8 × 10-2 μM-1 h-1, respectively, presumably because of the steric hindrance introduced by the PNIPAM chain. Moreover, after initiating the oligomerization, we found that the oligomer distribution in the first 6 h repeatedly and quantitatively follows the Smoluchowski coagulation model. Our study paves the way for controllably and quantitatively studying the oligomerization kinetics of amyloidogenic proteins. In addition, by investigating the effects of different small molecules on the oligomerization kinetics, we should be able to screen potential drugs to slow the development of neurodegenerative diseases.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Wang Y,Wu C

doi

10.1021/acs.biochem.7b00966

subject

Has Abstract

pub_date

2017-12-19 00:00:00

pages

6575-6584

issue

50

eissn

0006-2960

issn

1520-4995

journal_volume

56

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Differential regulation of oxidative and osmotic stress induced Syk activation by both autophosphorylation and SH2 domains.

    abstract：:Syk, a nonreceptor protein-tyrosine kinase, is activated by both oxidative and osmotic stress and plays different roles in the transduction of stress signals. In this study, the regulation of oxidative and osmotic stress induced Syk activation was investigated utilizing Syk-negative DT40 cells, expressing various Syk ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9729460

    authors： Qin S,Kurosaki T,Yamamura H

    更新日期：1998-04-21 00:00:00

  • Electrochemical proton gradient across the cell membrane of Halobacterium halobium: comparison of the light-induced increase with the increase of intracellular adenosine triphosphate under steady-state illumination.

    abstract：:The increase of the proton motive force mediated by bacteriorhodopsin is compared to the intracellular ATP concentration under steady-state illumination. The membrane potential was measured via the accumulation of the lipophilic ion [14C]triphenylmethylphosphonium and the pH gradient via the accumulation of the weak a...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00561a012

    authors： Michel H,Oesterhelt D

    更新日期：1980-09-30 00:00:00

  • Subunit structure of human erythrocyte glycophorin A.

    abstract：:Glycophorin A is a sialoglycoprotein isolated from human erythrocyte membranes which seems to exist as stable dimeric complexes in the presence of sodium dodecyl sulfate. When analyzed by dodecyl sulfate acrylamide electrophoresis this molecule forms two PAS-stainable bands (PAS-U and PAS-2) which are reversibly inter...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00650a028

    authors： Furthmayr H,Marchesi VT

    更新日期：1976-03-09 00:00:00

  • Structural and functional analysis of the transmembrane segment pair VI and VII of the NHE1 isoform of the Na+/H+ exchanger.

    abstract：:Isoform 1 of the mammalian Na(+)/H(+) exchanger (NHE1) is a ubiquitously expressed plasma membrane pH regulatory protein. It removes one intracellular H(+) in exchange for one extracellular Na(+). The 500 N-terminal amino acids comprise the catalytic membrane domain and fold into 12 transmembrane (TM) segments. To gai...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi500392y

    authors： Alves C,Lee BL,Sykes BD,Fliegel L

    更新日期：2014-06-10 00:00:00

  • Resonance Raman investigation of nitric oxide bonding in nitrosylhemoglobin A and -myoglobin: detection of bound N-O stretching and Fe-NO stretching vibrations from the hexacoordinated NO-heme complex.

    abstract：:With excitation at 406.7 nm, we have observed the resonance Raman enhancement of the bound v(N-O) stretch at approximately 1623 cm-1 in nitrosylhemoglobin A and nitrosylmyoglobin, indicating the existence of a charge-transfer transition underlying the strong Soret band. The v(Fe-NO) stretch at 551 cm-1 has also been d...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00535a005

    authors： Tsubaki M,Yu NT

    更新日期：1982-03-16 00:00:00

  • Identification of peptide antagonists to glycoprotein Ibalpha that selectively inhibit von Willebrand factor dependent platelet aggregation.

    abstract：:GPIbalpha is an integral membrane protein of the GPIb-IX-V complex found on the platelet surface that interacts with the A1 domain of von Willebrand factor (vWF-A1). The interaction of GPIbalpha with vWF-A1 under conditions of high shear stress is the first step in platelet-driven thrombus formation. Phage display was...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi702428q

    authors： Benard SA,Smith TM,Cunningham K,Jacob J,DeSilva T,Lin L,Shaw GD,Kriz R,Kelleher KS

    更新日期：2008-04-22 00:00:00

  • Mechanism of nucleotide release from Rho by the GDP dissociation stimulator protein.

    abstract：:Guanine nucleotide dissociation stimulator (GDS) promotes the release of tightly bound GDP from various Ras superfamily proteins, including RhoA, Rac1, K-Ras, Rap1A, and Rap1B. It displays no significant sequence homology to other known exchange factors for small G-proteins. Studies are reported here of the mechanism ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0007573

    authors： Hutchinson JP,Eccleston JF

    更新日期：2000-09-19 00:00:00

  • Role of the cochaperone Tpr2 in Hsp90 chaperoning.

    abstract：:The molecular chaperones Hsp90 and Hsp70 are highly regulated by various cochaperones that participate in the activation of steroid receptors. Here we study Tpr2 (also called DjC7), a TPR domain-containing type III J protein implicated in steroid receptor chaperoning. We propose that Tpr2 plays a role in the Hsp90-dep...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi800770g

    authors： Moffatt NS,Bruinsma E,Uhl C,Obermann WM,Toft D

    更新日期：2008-08-05 00:00:00

  • Crystal structures of the inactive D30N mutant of feline immunodeficiency virus protease complexed with a substrate and an inhibitor.

    abstract：:Crystal structures of complexes of a D30N mutant of feline immunodeficiency virus protease (FIV PR) complexed with a statine-based inhibitor (LP-149), as well as with a substrate based on a modification of this inhibitor (LP-149S), have been solved and refined at resolutions of 2.0 and 1.85 A, respectively. Both the i...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9707436

    authors： Laco GS,Schalk-Hihi C,Lubkowski J,Morris G,Zdanov A,Olson A,Elder JH,Wlodawer A,Gustchina A

    更新日期：1997-09-02 00:00:00

  • N-(5-Phosphoribosyl)anthranilate isomerase-indoleglycerol-phosphate synthase. 2. Fast-reaction studies show that a fluorescent substrate analogue binds independently to two different sites.

    abstract：:The mechanism of binding of reduced 1-(2-carboxyphenylamino)-1-deoxyribulose 5-phosphate (rCdRP) to two different binding sites on the bifunctional enzyme is determined by kinetic studies, using temperature-jump and stopped-flow equipment with fluorescence detection. Two rapid binding processes and a comparatively slo...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00593a030

    authors： Cohn W,Kirschner K,Paul C

    更新日期：1979-12-25 00:00:00

  • Evidence for a tRNA/rRNA interaction site within the peptidyltransferase center of the Escherichia coli ribosome.

    abstract：:A nine-base oligodeoxyribonucleotide complementary to bases 2497-2505 of 23S rRNA was hybridized to both 50S subunits and 70S ribosomes. The binding of the probe to the ribosome or ribosomal subunits was assayed by nitrocellulose filtration and by sucrose gradient centrifugation techniques. The location of the hybridi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00428a073

    authors： Marconi RT,Hill WE

    更新日期：1989-01-24 00:00:00

  • An RNA polymerase mutant with reduced accuracy of chain elongation.

    abstract：:A new Escherichia coli RNA polymerase mutant was isolated which exhibited reduced accuracy of chain elongation in vivo and in vitro. The novel isolation procedure consisted of simultaneous selection for rifampicin resistance and screening for increased leakiness of an early, strongly polar nonsense mutation of lacZ, o...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00368a013

    authors： Blank A,Gallant JA,Burgess RR,Loeb LA

    更新日期：1986-10-07 00:00:00

  • Generation and Comparative Kinetic Analysis of New Glycosynthase Mutants from Streptococcus pyogenes Endoglycosidases for Antibody Glycoengineering.

    abstract：:Chemoenzymatic glycan remodeling by endoglycosidase-catalyzed deglycosylation and reglycosylation is emerging as an attractive approach for producing homogeneous glycoforms of antibodies, and the success of this approach depends on the discovery of efficient endoglycosidases and their glycosynthase mutants. We report ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b00719

    authors： Tong X,Li T,Li C,Wang LX

    更新日期：2018-09-04 00:00:00

  • High-pressure 31P NMR study of dipalmitoylphosphatidylcholine bilayers.

    abstract：:High-pressure 31P NMR was used for the first time to investigate the effects of pressure on the structure and dynamics of the phosphocholine headgroup in pure 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) multilamellar aqueous dispersions and in DPPC bilayers containing the positively charged form of the local an...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00143a004

    authors： Peng X,Jonas J

    更新日期：1992-07-21 00:00:00

  • Binding of copper and silver to single-site variants of peptidylglycine monooxygenase reveals the structure and chemistry of the individual metal centers.

    abstract：:Peptidylglycine monooxygenase (PHM) catalyzes the final step in the biosynthesis of amidated peptides that serve as important signaling molecules in numerous endocrine pathways. The catalytic mechanism has attracted much attention because of a number of unique attributes, including the presence of a pair of uncoupled ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi4015264

    authors： Chauhan S,Kline CD,Mayfield M,Blackburn NJ

    更新日期：2014-02-18 00:00:00

  • The function of the chloride ion in photosynthetic oxygen evolution.

    abstract：:The involvement of Cl(-) and several other monovalent anions in photosynthetic oxygen evolution was studied using photosystem II membranes depleted of Cl(-) by dialysis. The results of these studies differ significantly from results obtained using other depletion methods. Binding studies with glycerol as a cryoprotect...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi026175y

    authors： Olesen K,Andréasson LE

    更新日期：2003-02-25 00:00:00

  • Interaction of C225SR1 mutant subunit of ribonucleotide reductase with R2 and nucleoside diphosphates: tales of a suicidal enzyme.

    abstract：:Ribonucleotide reductase (RDPR) from Escherichia coli is composed of two subunits, R1 and R2, both of which are required to catalyze the conversion of nucleotides to deoxynucleotides. This reduction process is accompanied by oxidation of two cysteines within the active site to a disulfide. One of these putative active...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00155a030

    authors： Mao SS,Holler TP,Bollinger JM Jr,Yu GX,Johnston MI,Stubbe J

    更新日期：1992-10-13 00:00:00

  • Characterization of the N-terminal and C-terminal domains of human apolipoprotein(a): relevance to fibrin binding.

    abstract：:The structural domains of human apolipoprotein(a) (apo(a)) in the lipoprotein(a) (Lp(a)) particle have been recently investigated by limited proteolysis [Huby, T., Doucet, C., Dieplinger, H., Chapman, J., & Thillet, J. (1994) Biochemistry 33, 3335-3341]. We have shown that apo(a) can be cleaved into two structural dom...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00022a011

    authors： Huby T,Schröder W,Doucet C,Chapman J,Thillet J

    更新日期：1995-06-06 00:00:00

  • Effects of peptide length and composition on binding to an empty class I MHC heterodimer.

    abstract：:Class I major histocompatibility complex (MHC) proteins present peptide antigens to T cells during the immune response against viruses. Peptides are loaded into newly synthesized class I heterodimers in the endoplasmic reticulum such that most or all cell surface class I molecules contain peptides derived from endogen...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00192a020

    authors： Fahnestock ML,Johnson JL,Feldman RM,Tsomides TJ,Mayer J,Narhi LO,Bjorkman PJ

    更新日期：1994-07-05 00:00:00

  • Solid-phase synthesis and side reactions of oligonucleotides containing O-alkylthymine residues.

    abstract：:As part of our studies on the molecular mechanism of mutation [Chambers, R. W. (1982) in Molecular and Cellular Mechanisms of Mutagenesis (Lemontt, J. F., & Generoso, W. M., Eds.) pp 121-145, Plenum, New York and London], we wanted to prepare specific oligonucleotides carrying O2- or O4-alkylthymidine residues. Since ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00430a007

    authors： Borowy-Borowski H,Chambers RW

    更新日期：1989-02-21 00:00:00

  • High-yield functional expression of human sodium/d-glucose cotransporter1 in Pichia pastoris and characterization of ligand-induced conformational changes as studied by tryptophan fluorescence.

    abstract：:Studies on the structure-function relationship of transporters require the availability of sufficient amounts of the protein in a functional state. In this paper, we report the functional expression, purification, and reconstitution of the human sodium/d-glucose cotransporter1 (hSGLT1) in Pichia pastoris and ligand-in...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi051377q

    authors： Tyagi NK,Goyal P,Kumar A,Pandey D,Siess W,Kinne RK

    更新日期：2005-11-29 00:00:00

  • Significant catalytic roles for Glu47 and Gln 110 in all four of the C-C bond-making and -breaking steps of the reactions of acetohydroxyacid synthase II.

    abstract：:Acetohydroxy acid synthase (AHAS) is a thiamin diphosphate (ThDP)-dependent enzyme that catalyzes the first common step in the biosynthesis of branched-chain amino acids, condensation of pyruvate with a second 2-ketoacid to form either acetolactate or acetohydroxybutyrate. AHAS isozyme II from Escherichia coli is spec...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi102051h

    authors： Vyazmensky M,Steinmetz A,Meyer D,Golbik R,Barak Z,Tittmann K,Chipman DM

    更新日期：2011-04-19 00:00:00

  • Identification of a novel noncatalytic bicarbonate binding site in eubacterial beta-carbonic anhydrase.

    abstract：:The structures of beta class carbonic anhydrases (beta-CAs) determined so far fall into two distinct subclasses based on the observed coordination of the catalytic zinc (Zn2+) ion. The subclass of beta-CAs that coordinate Zn2+ tetrahedrally with four protein-derived ligands is represented by the structures of ortholog...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi052272q

    authors： Cronk JD,Rowlett RS,Zhang KY,Tu C,Endrizzi JA,Lee J,Gareiss PC,Preiss JR

    更新日期：2006-04-11 00:00:00

  • Stimulation of iron(II) bleomycin activity by phosphate-containing compounds.

    abstract：:Orthophosphate and phosphate derivatives including pyrophosphate, hexametaphosphate, ATP, ADP, and inositol hexaphosphate enhance the extent of DNA degradation by iron(II) bleomycin. These phosphate-containing compounds increase both the release of free nucleic base and that of base propenals which are DNA cleavage pr...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00335a034

    authors： Burger RM,Horwitz SB,Peisach J

    更新日期：1985-07-02 00:00:00

  • Thermal unfolding and aggregation of human complement protein C9: a differential scanning calorimetry study.

    abstract：:The thermotropic behavior of purified human complement protein C9 was investigated by high-sensitivity differential scanning calorimetry. When dissolved in physiological buffers (pH 7.2, 150 mM NaCl), C9 underwent three endothermic transitions with transition temperatures (Tm) centered at about 32, 48, and 53 degrees ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00240a035

    authors： Lohner K,Esser AF

    更新日期：1991-07-02 00:00:00

  • All three LDL receptor homology regions of the LDL receptor-related protein bind multiple ligands.

    abstract：:The three complete human LDL receptor homology regions of the LDL receptor-related protein (sLRP2, sLRP3, and sLRP4) have been expressed in Pichia pastoris SMD1168 with constitutive coexpression of the receptor-associated protein (RAP). Each sLRP was purified to homogeneity after deglycosylation using a combination of...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi034752s

    authors： Croy JE,Shin WD,Knauer MF,Knauer DJ,Komives EA

    更新日期：2003-11-11 00:00:00

  • Structural analysis of sterol distributions in the plasma membrane of living cells.

    abstract：:Although plasma membrane (PM) cholesterol-rich and -poor domains have been isolated by subcellular fractionation, the real-time arrangement of cholesterol in such domains in living cells is still unclear. Therefore, dehydroergosterol (DHE), a naturally occurring fluorescent sterol, was incorporated into cultured L-cel...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi048172m

    authors： Zhang W,McIntosh AL,Xu H,Wu D,Gruninger T,Atshaves B,Liu JC,Schroeder F

    更新日期：2005-03-01 00:00:00

  • Molecular cloning of three distinct forms of the Na+,K+-ATPase alpha-subunit from rat brain.

    abstract：:Rat brain and kidney cDNA libraries were constructed and screened with a cDNA insert corresponding to the mRNA for the sheep kidney Na+,K+-ATPase catalytic subunit. The alpha-subunit cDNAs isolated from the kidney library were derived from a single class of messenger RNA, and the brain cDNAs were derived from three cl...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00373a001

    authors： Shull GE,Greeb J,Lingrel JB

    更新日期：1986-12-16 00:00:00

  • An actin-binding site on the 20K fragment of myosin subfragment 1.

    abstract：:Myosin subfragment 1 (S1) was covalently labeled with a fluorescent dye, N-[7-(dimethylamino)-4-methyl-3-coumarinyl]maleimide (DACM), and then digested by trypsin to cleave S1 heavy chain into fragments. The DACM-labeled and trypsin-treated S1 was complexed with F-actin and treated with a zero-length cross-linker, 1-e...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00262a043

    authors： Sutoh K

    更新日期：1982-09-14 00:00:00

  • Functional interactions of recombinant alpha 2 adrenergic receptor subtypes and G proteins in reconstituted phospholipid vesicles.

    abstract：:The functional interaction of the recombinant alpha 2 adrenergic receptor subtypes, alpha 2-C10 (the human platelet alpha 2 receptor, equivalent to the alpha 2 A subtype) and alpha 2-C4 (an alpha 2 receptor subtype cloned from a human kidney cDNA library), with G proteins was characterized in an in vitro reconstitutio...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00227a024

    authors： Kurose H,Regan JW,Caron MG,Lefkowitz RJ

    更新日期：1991-04-02 00:00:00