Electrochemical proton gradient across the cell membrane of Halobacterium halobium: comparison of the light-induced increase with the increase of intracellular adenosine triphosphate under steady-state illumination.

abstract：:In order to clarify the functional and structural role of intra- and intersubunit hydrogen bonds in human hemoglobin (Hb A), we prepared two artificial beta chain mutant hemoglobins by site-directed mutagenesis. The mutant Hb Phe-37 beta, in which Trp-37 beta is replaced by Phe to remove the intersubunit hydrogen bond...

journal_title：Biochemistry

authors： Ishimori K,Imai K,Miyazaki G,Kitagawa T,Wada Y,Morimoto H,Morishima I

更新日期：1992-03-31 00:00:00

abstract：:Double homeodomain (DUX) proteins are encoded by a family of 3.3-kilobase repeated elements dispersed in the human genome. One of these elements named D4Z4 is found in a tandem repeat array on chromosome 4 that is partially deleted in facioscapulohumeral muscular dystrophy. We have evaluated the trafficking and mobili...

journal_title：Biochemistry

authors： Ostlund C,Garcia-Carrasquillo RM,Belayew A,Worman HJ

更新日期：2005-02-22 00:00:00

abstract：:The cystic fibrosis transmembrane conductance regulator (CFTR) functions in vivo as a cAMP-activated chloride channel. A member of the ATP-binding cassette superfamily of membrane transporters, CFTR contains two transmembrane domains (TMDs), two nucleotide-binding domains (NBDs), and a regulatory (R) domain. It is pre...

journal_title：Biochemistry

authors： Ketchum CJ,Rajendrakumar GV,Maloney PC

更新日期：2004-02-03 00:00:00

abstract：:X-ray absorption spectroscopy has been used to investigate binding of selenohomocysteine to cobalamin-independent (MetE) and cobalamin-dependent (MetH) methionine synthase enzymes of Escherichia coli. We have shown previously [Peariso et al. (1998) J. Am. Chem. Soc. 120, 8410-8416] that the Zn sites in both enzymes sh...

journal_title：Biochemistry

authors： Peariso K,Zhou ZS,Smith AE,Matthews RG,Penner-Hahn JE

更新日期：2001-01-30 00:00:00

abstract：:The association of membrane and cytoplasmic proteins with the cytoskeleton of resting and activated platelets was studied. Glycoproteins were identified by labeling with 125I-labeled lectins (concanavalin A, wheat germ agglutinin, and Lens culinaris). Polypeptides, which are embedded in the lipid bilayer, have been id...

journal_title：Biochemistry

authors： Rotman A,Heldman J,Linder S

更新日期：1982-04-13 00:00:00

abstract：:The Haemophilus influenzae diaminopimelate epimerase was cloned, expressed, purified, and crystallized in the C2221 space group (a = 102.1 A, b = 115.4 A, c = 66.3 A, alpha = beta = gamma = 90 degrees). The three-dimensional structure was solved to 2.7 A using a single Pt derivative and the Se-Met-substituted enzyme t...

journal_title：Biochemistry

authors： Cirilli M,Zheng R,Scapin G,Blanchard JS

更新日期：1998-11-24 00:00:00

abstract：:In cells, RNAs likely adopt numerous intermediate conformations prior to formation of functional RNA-protein complexes. We used single-nucleotide resolution selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE) to probe the structure of Escherichia coli 16S rRNA in healthy growing bacteria. SHAPE-direct...

journal_title：Biochemistry

authors： McGinnis JL,Weeks KM

更新日期：2014-05-20 00:00:00

abstract：:Apurinic/apyrimidinic endonuclease 1 (APE1) is a DNA repair protein and plays other important roles. Increased levels of APE1 in cancer have been reported. However, available methods for measuring APE1 levels are indirect and not quantitative. We previously developed an approach using liquid chromatography and tandem ...

journal_title：Biochemistry

authors： Coskun E,Jaruga P,Reddy PT,Dizdaroglu M

更新日期：2015-09-29 00:00:00

abstract：:The effects of an inhibitory monoclonal antibody (mAb) raised against the beta subunit of the Escherichia coli RNA polymerase were determined on the kinetics and structural interactions during formation of the open promoter complex (RPo). Analysis of the kinetics of abortive initiation on linear and supercoiled templa...

journal_title：Biochemistry

authors： Rockwell P,Krakow JS

更新日期：1988-05-03 00:00:00

abstract：:Protein splicing is a self-catalyzed post-translational modification in which the intein enzyme excises itself from a precursor protein and ligates the flanking sequences to produce a mature protein. We report the solution structure of a 136-residue DnaX mini-intein enzyme derived from the cyanobacterium Spirulina pla...

journal_title：Biochemistry

authors： Boral S,Maiti S,Basak AJ,Lee W,De S

更新日期：2020-12-22 00:00:00

abstract：:The cyclotides constitute a recently discovered family of plant-derived peptides that have the unusual features of a head-to-tail cyclized backbone and a cystine knot core. These features are thought to contribute to their exceptional stability, as qualitatively observed during experiments aimed at sequencing and char...

journal_title：Biochemistry

authors： Colgrave ML,Craik DJ

更新日期：2004-05-25 00:00:00

abstract：:Specific lysine residues on the ubiquitin surface were selected during the course of evolution to form different polyubiquitin chain structures that signal diverse cellular processes. A vast number of ubiquitin receptors specifically recognize and decode the signals conferred by these polyubiquitin chains. The mechani...

journal_title：Biochemistry

authors： Levin-Kravets O,Shohat N,Prag G

更新日期：2015-08-04 00:00:00

abstract：:The molecular dynamics of binary dispersions of plasmenylcholine/cholesterol and phosphatidylcholine/cholesterol were quantified by electron spin resonance (ESR) and deuterium magnetic resonance (2H NMR) spectroscopy. The order parameter of both 5-doxylstearate (5DS) and 16-doxylstearate (16DS) was larger in vesicles ...

journal_title：Biochemistry

authors： Pak JH,Bork VP,Norberg RE,Creer MH,Wolf RA,Gross RW

更新日期：1987-07-28 00:00:00

abstract：:While two-component systems (TCSs), composed of a sensor histidine kinase (HK) and a response regulator, are the main signaling pathways in bacteria, global TCS activity remains poorly described. Here, we report the kinetic parameters of the HK autophosphorylation reaction using previously uncharacterized γ-phosphate-...

journal_title：Biochemistry

authors： Chase OM,Espinasse A,Wilke KE,Carlson EE

更新日期：2018-07-24 00:00:00

abstract：:Mitochondrial thiolase I from pig heart has been found to have at least two and possibly three reactive sulfhydryl residues at or near the active site [Izbicka-Dimitrijević, E., & Gilbert, H. F. (1982) Biochemistry 21, 6112-6118; Izbicka-Dimitrijević, E., & Gilbert, H. F. (1984) Biochemistry 23, 4318-4324]. In the nat...

journal_title：Biochemistry

authors： Izbicka E,Gilbert HF

更新日期：1984-12-18 00:00:00

abstract：:Insulin-stimulated glucose disposal in skeletal muscle proceeds predominantly through a nonoxidative pathway with glycogen synthase as a rate-limiting enzyme, yet the mechanisms for insulin activation of glycogen synthase are not understood despite years of investigation. Isolation of putative insulin second messenger...

journal_title：Biochemistry

authors： Brautigan DL,Brown M,Grindrod S,Chinigo G,Kruszewski A,Lukasik SM,Bushweller JH,Horal M,Keller S,Tamura S,Heimark DB,Price J,Larner AN,Larner J

更新日期：2005-08-23 00:00:00

abstract：:Four new in vivo metabolites of vitamin D3 were isolated from the blood plasma of chicks given large doses of vitamin D3. The metabolites were isolated by methanol-chloroform extraction and a series of chromatographic procedures. By use of mass spectrometry, ultraviolet absorption spectrophotometry, and specific chemi...

journal_title：Biochemistry

authors： Wichmann JK,Schnoes HK,DeLuca HF

更新日期：1981-12-22 00:00:00

abstract：:Muscarinic cholinergic receptors can appear to be more numerous when labeled by [(3)H]quinuclidinylbenzilate (QNB) than by N-[(3)H]methylscopolamine (NMS). The nature of the implied heterogeneity has been studied with M(2) receptors in detergent-solubilized extracts of porcine atria. The relative capacity for [(3)H]NM...

journal_title：Biochemistry

authors： Park PS,Sum CS,Pawagi AB,Wells JW

更新日期：2002-04-30 00:00:00

abstract：:Proteorhodopsins are a recently discovered class of microbial rhodopsins, ubiquitous in marine bacteria. Over 4000 variants have thus far been discovered, distributed throughout the oceans of the world. Most variants fall into one of two major groups, green- or blue-absorbing proteorhodopsin (GPR and BPR, respectively...

journal_title：Biochemistry

authors： Kralj JM,Bergo VB,Amsden JJ,Spudich EN,Spudich JL,Rothschild KJ

更新日期：2008-03-18 00:00:00

abstract：:Subunit II of the aa3 type cytochrome c oxidase contains a binuclear copper center (CuA) which functions as the entry point for electrons donated by cytochrome c. We have introduced site-specific mutations in residues liganding the CuA center in the oxidase of the bacterium Paracoccus denitrificans; the purified, full...

journal_title：Biochemistry

authors： Zickermann V,Wittershagen A,Kolbesen BO,Ludwig B

更新日期：1997-03-18 00:00:00

abstract：:Sterol carrier protein 2 like 2 from Aedes aegypti (AeSCP2L2) plays an important role in lipid transport in mosquitoes for its routine metabolic processes. Repeated unsuccessful attempts to crystallize ligand free SCP2L2 prompted us to undertake nuclear magnetic resonance (NMR) spectroscopy to determine its three-dime...

journal_title：Biochemistry

authors： Singarapu KK,Ahuja A,Potula PR,Ummanni R

更新日期：2016-09-06 00:00:00

abstract：:The repair of the endogenous lesion 8-oxo-7,8-dihydrodeoxyguanosine (8-oxodG) was investigated in the nucleotide excision repair mutant xeroderma pigmentosum D (XPD), using human normal or transformed XPD fibroblasts and the Chinese hamster XPD cell line UV5. In vivo repair of 8-oxodG induced by hydrogen peroxide trea...

journal_title：Biochemistry

authors： Cappelli E,Degan P,Thompson LH,Frosina G

更新日期：2000-08-29 00:00:00

abstract：:The hydrogen exchange behavior of the N-terminal helical segment in horse heart cytochrome c was studied in both the reduced and the oxidized forms by use of two-dimensional nuclear magnetic resonance methods. The amide protons of the first six residues are not H bonded and exchange rapidly with solvent protons. The m...

journal_title：Biochemistry

authors： Wand AJ,Roder H,Englander SW

更新日期：1986-03-11 00:00:00

abstract：:Extracellular cAMP induces the rapid activation of guanylate cyclase, which adapts within 10 s to constant cAMP concentrations. A new response can be induced either by a higher cAMP concentration or by the same cAMP concentration at some time (t1/2 = 90 s) after removal of the previous stimulus. Stimulation of guanyla...

journal_title：Biochemistry

authors： Van Haastert PJ

更新日期：1987-11-17 00:00:00

abstract：:Amyloid diseases are caused by the self-assembly of a given protein into an insoluble cross-beta-sheet quaternary structural form which is pathogenic. An understanding of the biochemical mechanism of amyloid fibril formation should prove useful in understanding amyloid disease. Toward this end, a procedure for the con...

journal_title：Biochemistry

authors： Colon W,Kelly JW

更新日期：1992-09-15 00:00:00

abstract：:Modulation of endogenous gene function, through sequence-specific recognition of double helical DNA via oligonucleotide-directed triplex formation, is a promising approach. Compared to the formation of pyrimidine motif triplexes, which require relatively low pH, purine motif appears to be the most gifted for their sta...

journal_title：Biochemistry

authors： Kaushik S,Kaushik M,Svinarchuk F,Malvy C,Fermandjian S,Kukreti S

更新日期：2011-05-17 00:00:00

abstract：:Membrane vesicles from Mycobacterium phlei contain carrier proteins for proline, glutamine, and glutamic acid. The transport of proline is Na+ dependent and required substrate oxidation. A proline carrier protein was solubilized from the membrane vesicles by treatment with cholate and Triton X-100. Electron microscopi...

journal_title：Biochemistry

authors： Lee SH,Cohen NS,Jacobs AJ,Brodie AF

更新日期：1979-05-29 00:00:00

abstract：:Structure formation and dimerization are concerted processes in the refolding of Arc repressor. The integrity of secondary structure in the transition state of Arc refolding has been investigated here by determining the changes in equilibrium stability and refolding/unfolding kinetics for a set of Ala --> Gly mutation...

journal_title：Biochemistry

authors： Srivastava AK,Sauer RT

更新日期：2000-07-18 00:00:00

abstract：:The stoichiometry of cytochrome b559 (one or two copies) per reaction center of photosystem II (PSII) has been the subject of considerable debate. The molar ratio of cytochrome b559 has a number of significant implications on our understanding of the functional role of cytochrome b559, the mechanism of electron donati...

journal_title：Biochemistry

authors： Buser CA,Diner BA,Brudvig GW

更新日期：1992-11-24 00:00:00

abstract：:The unique occurrence of G-quadruplexes in the AT-rich genome of human malaria parasite Plasmodium falciparum provides hints about their critical roles in parasite survival, pathogenesis, and host immune evasion. An intriguing question is whether these noncanonical structures can serve as molecular targets for small m...

journal_title：Biochemistry

authors： Anas M,Sharma R,Dhamodharan V,Pradeepkumar PI,Manhas A,Srivastava K,Ahmed S,Kumar N

更新日期：2017-12-26 00:00:00