Intracellular trafficking and dynamics of double homeodomain proteins.

abstract：:Gram-negative bacteria, such as Escherichia coli, utilize efflux resistance systems in order to expel toxins from their cells. Heavy-metal resistance is mediated by resistance nodulation cell division (RND)-based efflux pumps composed of a tripartite complex that includes an RND-transporter, an outer-membrane factor (...

journal_title：Biochemistry

authors： Mealman TD,Zhou M,Affandi T,Chacón KN,Aranguren ME,Blackburn NJ,Wysocki VH,McEvoy MM

更新日期：2012-08-28 00:00:00

abstract：:Low pH-induced membrane insertion by diphtheria toxin T domain is crucial for A chain translocation into the cytoplasm. To define the membrane topography of the T domain, the exposure of biotinylated Cys residues to the cis and trans bilayer surfaces was examined using model membrane vesicles containing a deeply inser...

journal_title：Biochemistry

authors： Rosconi MP,Zhao G,London E

更新日期：2004-07-20 00:00:00

abstract：:The gene product 61 primase protein from bacteriophage T4 was expressed as an intein fusion and purified to homogeneity. The primase binds one zinc ion, which is coordinated by four cysteine residues to form a zinc ribbon motif. Factors that influence the rate of priming were investigated, and a physiologically releva...

journal_title：Biochemistry

authors： Valentine AM,Ishmael FT,Shier VK,Benkovic SJ

更新日期：2001-12-18 00:00:00

abstract：:Applications of sensitive new technologies, in particular, two-dimensional NMR spectroscopy, have allowed detection of folded structures in short peptide fragments of proteins in aqueous solution under conditions where native proteins fold. These structures are in rapid dynamic exchange with unfolded states. These obs...

journal_title：Biochemistry

authors： Wright PE,Dyson HJ,Lerner RA

更新日期：1988-09-20 00:00:00

abstract：:The interaction of poly(N epsilon, N epsilon, N epsilon-trimethyl-L-lysine) ([Lys(Me3)]n) and poly(N delta, N delta, N delta-trimethyl-L-ornithine) ([Orn(Me3)]n) with polynucleotides was studied by thermal denaturation, viscosity, and dissociation by salt. The methylated polymers decrease the viscosity of DNA in propo...

journal_title：Biochemistry

authors： Granados EN,Bello J

更新日期：1980-07-08 00:00:00

abstract：:By following the kinetics of abortive and productive synthesis in single-round transcription assays, we confirm the existence of two general classes of initial transcribing complexes (ITCs), which we term "productive ITC" and "unproductive ITC". The productive ITCs are able to escape from the promoter rapidly to produ...

journal_title：Biochemistry

authors： Vo NV,Hsu LM,Kane CM,Chamberlin MJ

更新日期：2003-04-08 00:00:00

abstract：:The acetylenic analogue of adenosine 9-(5',6'-dideoxy-beta-D-ribo-hex-5'-ynofuranosyl)adenine has been synthesized, and its behavior as an inhibitor of bovine S-adenosylhomocysteine hydrolase has been examined. Incubation of the enzyme with excess inhibitor caused a time-dependent, irreversible inactivation of the enz...

journal_title：Biochemistry

authors： Parry RJ,Muscate A,Askonas LJ

更新日期：1991-10-15 00:00:00

abstract：:Molecular dynamics simulations employing a molecular mechanics (MM) force field and hybrid quantum mechanics (QM) and MM (QM/MM) have been carried out to investigate the product specificity and mechanism of the histone H4 lysine 20 (H4-K20) methylation by human histone lysine methyltransferase SET8. At neutral pH, the...

journal_title：Biochemistry

authors： Zhang X,Bruice TC

更新日期：2008-06-24 00:00:00

abstract：:Proteins performing multiple biochemical functions are called "moonlighting proteins" or extreme multifunctional (EMF) proteins. Mitochondrial cytochrome c is an EMF protein that binds multiple partner proteins to act as a signaling molecule, transfers electrons in the respiratory chain, and acts as a peroxidase in ap...

journal_title：Biochemistry

authors： Deacon OM,Karsisiotis AI,Moreno-Chicano T,Hough MA,Macdonald C,Blumenschein TMA,Wilson MT,Moore GR,Worrall JAR

更新日期：2017-11-21 00:00:00

abstract：:Much interest has centered on two surface loops in the motor domain to explain the differences in enzymatic and mechanical properties of myosin isoforms. We showed that two invariant lysines at the C-terminal end of loop 2, which is part of the actin-binding interface, are required to obtain actin activation [Joel et ...

journal_title：Biochemistry

authors： Joel PB,Sweeney HL,Trybus KM

更新日期：2003-08-05 00:00:00

abstract：:Copper thiosemicarbazones cause considerable oxidative stress. This effect may be related to their cytotoxicity. In the present work, the chemical and cellular properties of a new ligand, pyridoxal thiosemicarbazone (H2T), and its copper(II) chelate (CuT) are assessed. CuT is toxic to cultured Ehrlich ascites tumor ce...

journal_title：Biochemistry

authors： Byrnes RW,Mohan M,Antholine WE,Xu RX,Petering DH

更新日期：1990-07-31 00:00:00

abstract：:Moderate pressure accelerates hydride transfer catalyzed by yeast alcohol dehydrogenase, indicative of a large negative volume of activation [Cho and Northrop (1999) Biochemistry 38, 7470-7475]. A comparison of the effects of pressure on the oxidation of normal versus dideuteriobenzyl alcohol generates a monophasic de...

journal_title：Biochemistry

authors： Northrop DB,Cho YK

更新日期：2000-03-07 00:00:00

abstract：:We report that recombinant glia maturation factor (GMF), a 17-kDa brain protein, inhibits the activity of mitogen-activated protein (MAP) kinase in the test tube assay, in particular the ERK1/ERK2 isoforms. A preliminary phosphorylation of GMF by protein kinase A (PKA) dramatically increases its inhibitory effect by o...

journal_title：Biochemistry

authors： Zaheer A,Lim R

更新日期：1996-05-21 00:00:00

abstract：:Ribonucleotide reductase from Escherichia coli consists of two nonidentical subunits, named protein B1 (170 000) and protein B2 (87 000). We purified and characterized five monoclonal antibodies against B1 and three against B2 from hybridomas obtained by fusion of spleen cells from immunized mice and the myeloma cell ...

journal_title：Biochemistry

authors： Anderson A,Barlow T,Pontis E,Reichard P

更新日期：1986-02-25 00:00:00

abstract：:The beta chain 15-42 sequence of the fibrin(ogen) E region was implicated in heparin binding [Odrljin et al. (1996) Blood 88, 2050-2061]; whether heparin binds to other fibrin(ogen) regions remains to be clarified. To address this question, we studied the interaction of heparin with fibrinogen, fibrin, and their major...

journal_title：Biochemistry

authors： Yakovlev S,Gorlatov S,Ingham K,Medved L

更新日期：2003-07-01 00:00:00

abstract：:Integrin conformational changes mediate integrin activation and signaling triggered by intracellular molecules or extracellular ligands. Even though it is known that αβ transmembrane domain separation is required for integrin signaling, it is still not clear how this signal is transmitted from the transmembrane domain...

journal_title：Biochemistry

authors： Wang W,Fu G,Luo BH

更新日期：2010-11-30 00:00:00

abstract：:Lactoferrin (Lf) and serum transferrin (Tf) combine high-affinity iron binding with an ability to release this iron at reduced pH. Lf, however, retains iron to significantly lower pH than Tf, giving the two proteins distinct functional roles. In this paper, we compared the iron-release profiles for human Lf, Tf, and t...

journal_title：Biochemistry

authors： Peterson NA,Anderson BF,Jameson GB,Tweedie JW,Baker EN

更新日期：2000-06-06 00:00:00

abstract：:S100A6 is a member of the S100 subfamily of EF-hand Ca (2+) binding proteins that has been shown to interact with calcyclin binding protein/Siah-1 interacting protein (CacyBP/SIP or SIP), a subunit of an SCF-like E3 ubiquitin ligase complex (SCF-TBL1) formed under genotoxic stress. SIP serves as a scaffold in this com...

journal_title：Biochemistry

authors： Lee YT,Dimitrova YN,Schneider G,Ridenour WB,Bhattacharya S,Soss SE,Caprioli RM,Filipek A,Chazin WJ

更新日期：2008-10-14 00:00:00

abstract：:The nature of the putative general acid His187 in the reaction catalyzed by Escherichia coli uracil DNA glycosylase (UDG) was investigated using X-ray crystallography and NMR spectroscopy. The crystal structures of H187Q UDG, and its complex with uracil, have been solved at 1.40 and 1.60 A resolution, respectively. Th...

journal_title：Biochemistry

authors： Drohat AC,Xiao G,Tordova M,Jagadeesh J,Pankiewicz KW,Watanabe KA,Gilliland GL,Stivers JT

更新日期：1999-09-14 00:00:00

abstract：:A nucleoside triphosphate binding site on calf thymus RNA polymerase II was identified by using photoaffinity analogues of adenosine 5'-triphosphate and guanosine 5'-triphosphate. Both radiolabeled 8-azidoadenosine 5'-triphosphate (8-N3ATP) and radiolabeled 8-azidoguanosine 5'-triphosphate (8-N3GTP) bound to a single ...

journal_title：Biochemistry

authors： Freund E,McGuire PM

更新日期：1986-01-14 00:00:00

abstract：:Pharaonis halorhodopsin (phR), the light-driven chloride ion pump from Natronobacterium pharaonis with C-terminal histidine tag, was expressed in Escherichia coli cells. The protein was solubilized with 0.1% n-dodecyl beta-D-maltopyranoside and purified with a nickel column. Removal of Cl- from the medium yields blue ...

journal_title：Biochemistry

authors： Sato M,Kanamori T,Kamo N,Demura M,Nitta K

更新日期：2002-02-19 00:00:00

abstract：:The light-induced global conformational change of photoactive yellow protein was directly observed by small-angle X-ray scattering (SAXS). The N-terminal 6, 15, or 23 amino acid residues were enzymatically truncated (T6, T15, or T23, respectively), and their near-UV intermediates were accumulated under continuous illu...

journal_title：Biochemistry

authors： Imamoto Y,Kamikubo H,Harigai M,Shimizu N,Kataoka M

更新日期：2002-11-19 00:00:00

abstract：:A 16-base pair oligo(purine)-oligo(pyrimidine) sequence present in the coding region of two HIV 1 proviral genes (pol and nef) was chosen as a target for triplex-forming oligonucleotides in in vitro transcription assays. Inhibition of transcription elongation was observed with triplex-forming oligonucleotide-acridine ...

journal_title：Biochemistry

authors： Giovannangeli C,Perrouault L,Escudé C,Nguyen T,Hélène C

更新日期：1996-08-13 00:00:00

abstract：:Integrin beta subunits contain a highly conserved I-like domain that is known to be important for ligand binding. Unlike integrin I domains, the I-like domain requires integrin alpha and beta subunit association for optimal folding. Pactolus is a novel gene product that is highly homologous to integrin beta subunits b...

journal_title：Biochemistry

authors： Takagi J,DeBottis DP,Erickson HP,Springer TA

更新日期：2002-04-02 00:00:00

abstract：:The fluorescence quenching of 9-aminoacridine (9AA) in suspension of beef heart EDTA submitochondrial particles was studied and was used to calculate the pH gradient between these particles and the medium. This pH gradient, which is energy dependent, is also dependent strongly on the presence of anion species in the m...

journal_title：Biochemistry

authors： Rottenberg H,Lee CP

更新日期：1975-06-17 00:00:00

abstract：:Equine chorionic gonadotropin (eCG) and lutropin (eLH) are composed of alpha- and beta-subunits with an identical amino acid sequence but show different biological activities. To elucidate the molecular difference between these gonadotropins, the structure of the N-linked oligosaccharides of each beta-subunit was dete...

journal_title：Biochemistry

authors： Matsui T,Mizuochi T,Titani K,Okinaga T,Hoshi M,Bousfield GR,Sugino H,Ward DN

更新日期：1994-11-29 00:00:00

abstract：:All-trans-retinol is the precursor for all-trans-retinoic acid, the activating ligand for nuclear transcription factors retinoic acid receptors. In the cytosol of various cells, most retinol exists in a bound form, complexed with cellular retinol binding protein type I (holo-CRBP). Whether retinoic acid is produced fr...

journal_title：Biochemistry

authors： Lapshina EA,Belyaeva OV,Chumakova OV,Kedishvili NY

更新日期：2003-01-28 00:00:00

abstract：:Despite extensive characterization of the human immunodeficiency virus type 1 (HIV-1) hydrophobic fusion peptide (FP), the structure-function relationships underlying its extraordinary degree of conservation remain poorly understood. Specifically, the fact that the tandem repeat of the FLGFLG tripeptide is absolutely ...

journal_title：Biochemistry

authors： Serrano S,Huarte N,Rujas E,Andreu D,Nieva JL,Jiménez MA

更新日期：2017-10-17 00:00:00

abstract：:The Xenopus borealis somatic 5S ribosomal RNA gene was used as a model system to determine the mutual effects of nucleosome folding and formation of ultraviolet (UV) photoproducts (primarily cis-syn cyclobutane pyrimidine dimers, or CPDs) in chromatin. We analyzed the preferred rotational and translational settings of...

journal_title：Biochemistry

authors： Liu X,Mann DB,Suquet C,Springer DL,Smerdon MJ

更新日期：2000-01-25 00:00:00

abstract：:Reconstitution of highly purified aquaporin CHIP (channel-forming integral protein) into proteoliposomes was previously shown to confer high osmotic water permeability (Pf) to the membranes [Zeidel et al. (1992) Biochemistry 31, 7436-7440]. Here we report detailed ultrastructural, pharmacologic, and transport studies ...

journal_title：Biochemistry

authors： Zeidel ML,Nielsen S,Smith BL,Ambudkar SV,Maunsbach AB,Agre P

更新日期：1994-02-15 00:00:00