Abstract：
:Structure formation and dimerization are concerted processes in the refolding of Arc repressor. The integrity of secondary structure in the transition state of Arc refolding has been investigated here by determining the changes in equilibrium stability and refolding/unfolding kinetics for a set of Ala --> Gly mutations at residues that are solvent-exposed in the native Arc dimer. At some sites, reduced stability was caused primarily by faster unfolding, indicating that secondary structure at these positions is largely absent in the transition state. However, most of the Ala --> Gly substitutions in the alpha-helices of Arc and a triple mutant in the beta-sheet also resulted in decreased refolding rates, in some cases, accounting for the major fraction of thermodynamic destabilization. Overall, these results suggest that some regions of native secondary structure are present but incompletely formed in the transition state of Arc refolding and dimerization. Consolidation of this secondary structure, like close packing of the hydrophobic core, seems to occur later in the folding process. On average, Phi(F) values for the Ala --> Gly mutations were significantly larger than Phi(F) values previously determined for alanine-substitution mutants, suggesting that backbone interactions in the transition state may be stronger than side chain interactions. Mutations causing significant reductions in the Arc refolding rate were found to cluster in the central turn of alpha-helix A and in the first two turns of alpha-helix B. In the Arc dimer, these elements pack together in a compact structure, which might serve as nucleus for further folding.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Srivastava AK,Sauer RTdoi
10.1021/bi000423dsubject
Has Abstractpub_date
2000-07-18 00:00:00pages
8308-14issue
28eissn
0006-2960issn
1520-4995pii
bi000423djournal_volume
39pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:We have developed a system for targeting foreign DNA to hepatocytes in vitro using a soluble DNA carrier that takes advantage of receptor-mediated endocytosis to achieve internalization. The idea is based on the fact that hepatocytes possess a unique receptor that binds and internalizes galactose-terminal (asialo)glyc...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00403a008
更新日期：1988-02-09 00:00:00
abstract：:Two nucleoside diphosphate analogs, 3'-C-methyl-ADP and 3'-C-methyl-UDP, have been tested as substrate and/or allosteric effectors using the adenosylcobalamin-dependent ribonucleoside diphosphate reductase of Corynebacterium nephridii. Neither analog was a substrate for the reductase. However, they did function as all...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00160a035
更新日期：1992-11-17 00:00:00
abstract：:Aminoglycoside 2''-phosphotransferase IVa [APH(2'')-IVa] is a member of a family of bacterial enzymes responsible for medically relevant resistance to antibiotics. APH(2'')-IVa confers high-level resistance against several clinically used aminoglycoside antibiotics in various pathogenic Enterococcus species by phospho...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi200747f
更新日期：2011-07-19 00:00:00
abstract：:Mutation of Pro94 to phenylalanine or alanine significantly alters the redox properties of the type I copper center of amicyanin. Each mutation increases the redox midpoint potential (E(m)) value by at least 140 mV and shifts the pK(a) for the pH dependence of the E(m) value to a more acidic value. Atomic resolution (...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi049634z
更新日期：2004-07-27 00:00:00
abstract：:Mutagenesis and cross-linking measurements have identified specific contact interactions between the cytosolic and the transmembrane sequences of phospholamban (PLB) and the Ca-ATPase, and in conjunction with the high-resolution structures of PLB and the Ca-ATPase, have been used to construct models of the PLB-ATPase ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi035424v
更新日期：2004-01-20 00:00:00
abstract：:Proteolytic fragments of bovine testis calmodulin were obtained by limited proteolysis with trypsin or thrombin. Cadmium-113 NMR studies showed that the tryptic fragment encompassing Ca2+ binding domains III and IV (TR2C) gave rise to a spectrum identical with that of the native protein. Two thrombic fragments contain...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00279a001
更新日期：1983-05-10 00:00:00
abstract：:By following the kinetics of abortive and productive synthesis in single-round transcription assays, we confirm the existence of two general classes of initial transcribing complexes (ITCs), which we term "productive ITC" and "unproductive ITC". The productive ITCs are able to escape from the promoter rapidly to produ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0269613
更新日期：2003-04-08 00:00:00
abstract：:S100 proteins are small dimeric members of the EF-hand superfamily of Ca(2+) binding proteins thought to participate in mediating intracellular Ca(2+) signals by binding to and thereby regulating target proteins in a Ca(2+)-dependent manner. As dimer formation is crucial to S100 function, we applied a yeast two-hybrid...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi000257+
更新日期：2000-08-08 00:00:00
abstract：:In an effort to better understand protein-protein photoaffinity cross-linking using aryl azides, we have tested a number of factors influencing the cross-linking of the sigma 70 subunit of Escherichia coli RNA polymerase to core RNA polymerase. These factors include the effect of the incubations necessary for the deri...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00206a012
更新日期：1994-10-11 00:00:00
abstract：:In prokaryotes, a large share of the proteins are secreted from the cell through a process that requires their translocation across the cytoplasmic membrane. This process is mediated by the universally conserved Sec system with homologues in the endoplasmic reticulum and thylakoid membranes of eukaryotes. The Sec syst...
journal_title：Biochemistry
pub_type： 杂志文章,评审
doi：10.1021/bi400913x
更新日期：2013-10-01 00:00:00
abstract：:In refolding of iso-2 cytochrome c from Saccharomyces cerevisiae, there are two slow folding reactions, tau 1a and tau 1b. The slower of the slow reactions, tau 1a = 100-200 s, is observed only by absorbance changes, while tau 1b (10-20-fold faster) is detected by fluorescence changes. The temperature dependence of th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00348a024
更新日期：1985-12-31 00:00:00
abstract：:The calcitonin/CGRP gene pre-mRNA is alternatively spliced in a tissue-specific manner resulting in the formation of calcitonin mRNA in thyroid C-cells and CGRP mRNA in neurons. Computer analysis of the RNA containing the 3' splice acceptor of the calcitonin-specific exon 4 predicts that this region has the potential ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9808058
更新日期：1998-11-10 00:00:00
abstract：:An amplified Okayama-Berg plasmid cDNA library was constructed from total poly(A)+ RNA isolated from the Madin-Darby bovine kidney cell line MDBK. This library was screened with a partial murine calpactin I heavy chain (p36) cDNA clone, the identification of which was based on bovine p36 tryptic peptide sequences gene...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00364a007
更新日期：1986-08-12 00:00:00
abstract：:Beta-1,4-galactosyltransferase (beta4Gal-T1) in the presence of manganese ion transfers galactose from UDP-galactose (UDP-Gal) to N-acetylglucosamine (GlcNAc) that is either free or linked to an oligosaccharide. Crystallographic studies on bovine beta4Gal-T1 have shown that the primary metal binding site is located in...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi049007+
更新日期：2004-10-05 00:00:00
abstract：:An IscA homologue within the nif regulon of Azotobacter vinelandii, designated (Nif)IscA, was expressed in Escherichia coli and purified to homogeneity. Purified (Nif)IscA was found to be a homodimer of 11-kDa subunits that contained no metal centers or other prosthetic groups in its as-isolated form. Possible roles f...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi015656z
更新日期：2001-11-20 00:00:00
abstract：:This study presents the first detailed examination by resonance Raman (RR) spectroscopy of the rates of solvent exchange for the C5 and C3 positions of the TPQ cofactor in several wild-type copper-containing amine oxidases and mutants of the amine oxidase from Hansenula polymorpha (HPAO). On the basis of crystal struc...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi011685y
更新日期：2002-01-15 00:00:00
abstract：:The rubredoxin from Clostridium pasteurianum contains a single iron atom bound to the polypeptide chain by cysteines 6, 9, 39, and 42. The C42A variant of this protein has been prepared by site-directed mutagenesis and heterologous expression of the gene in Escherichia coli. The mutated protein was found to contain an...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi971775w
更新日期：1997-10-28 00:00:00
abstract：:The self-assembly of beta(2)-microglobulin into fibrils leads to dialysis-related amyloidosis. pH-mediated partial unfolding is required for the formation of the amyloidogenic intermediate that then self-assembles into amyloid fibrils. Two partially folded intermediates of beta(2)-microglobulin have been identified ex...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi050731h
更新日期：2005-12-13 00:00:00
abstract：:The ATP-hydrolyzing portion of the proton pump from clathrin-coated vesicles (isolated from calf brain) was solubilized with three nondenaturing detergents (cholate, octyl glucoside, and Triton X-100). The hydrodynamic properties of the solubilized (Mg2+)-ATPase were then determined by sedimentation analysis in H2O an...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00363a015
更新日期：1986-07-29 00:00:00
abstract：:The Ogg1 protein of Saccharomyces cerevisiae belongs to a family of DNA glycosylases and apurinic/apyrimidinic site (AP) lyases, the signature of which is the alpha-helix-hairpin-alpha-helix-Gly/Pro-Asp (HhH-GPD) active site motif together with a conserved catalytic lysine residue, to which we refer as the HhH-GPD/K f...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi992262n
更新日期：2000-02-22 00:00:00
abstract：:Staphylococcal nuclease H124L is a recombinant protein produced in Escherichia coli whose sequence is identical with that of the nuclease produced by the V8 variant of Staphylococcus aureus. The enzyme-metal ion activator-nucleotide inhibitor ternary complex, nuclease H124L-thymidine 3',5'-bisphosphate-Ca2+, was inves...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00453a011
更新日期：1990-01-09 00:00:00
abstract：:Pyrimidine oligonucleotides have been shown to prime the E. coli DNA polymerase I repair reaction, specifically and reproducibly. DNA molecules up to 30 nucleotides long have been obtained from the extension of oligopyrimidine primers, 9 to 11 nucleotides long isolated from the complementary (minus) strand of bacterio...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00598a015
更新日期：1978-03-07 00:00:00
abstract：:The constant fragment of the immunoglobulin light chain in which the intramolecular disulfide bond is reduced (reduced CL fragment) assumes a conformation very similar to that of the intact CL fragment and contains two sulfhydryl groups buried in the interior of the molecule [Goto, Y., & Hamaguchi, K. (1979) J. Bioche...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00358a013
更新日期：1986-05-20 00:00:00
abstract：:Cdc31, the Saccharomyces cerevisiae centrin, is an EF-hand calcium-binding protein essential for the cell division and mRNA nuclear export. We used biophysical techniques to investigate its calcium, magnesium, and protein target binding properties as well as their conformations in solution. We show here that Cdc31 dis...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi200518d
更新日期：2011-07-26 00:00:00
abstract：:myo-Inositol oxygenase (MIOX) uses iron as its cofactor and dioxygen as its cosubstrate to effect the unique, ring-cleaving, four-electron oxidation of its cyclohexan-(1,2,3,4,5,6-hexa)-ol substrate to d-glucuronate. The nature of the iron cofactor and its interaction with the substrate, myo-inositol (MI), have been p...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0519607
更新日期：2006-05-02 00:00:00
abstract：:Segregation of mRNAs in the cytoplasm of polar cells has been demonstrated for proteins involved in Xenopus and Drosophila oogenesis, and for some proteins in somatic cells. It is assumed that vectorial transport of the messages is generally responsible for this localization. The mRNA encoding the basic protein of cen...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9800247
更新日期：1998-05-12 00:00:00
abstract：:The reactions of metmyoglobin (metMb) and methemoglobin (metHb), oxidized to their respective oxoferryl free radical species (.Mb-FeIV = O/.Hb-4FeIV = O) by tert-butyl hydroperoxide (t-BuOOH), with nitric oxide (NO.) were studied by a combination of optical, electron spin resonance (ESR), ionspray mass (MS), fluoresce...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00020a014
更新日期：1995-05-23 00:00:00
abstract：:The nuclear transport of the chromatin remodeling protein nucleoplasmin and chromatin building histones is mediated by importins. Nucleoplasmin (NP) contains a classical bipartite nuclear localization signal (NLS) that is recognized by the importin α/β heterodimer, while histones present multiple NLS-like motifs that ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi2008867
更新日期：2011-08-23 00:00:00
abstract：:Actin depolymerizing activity in serum can be attributed to the two proteins brevin and vitamin D binding protein (DBP). To investigate their mechanisms of action, we used a number of techniques, including procedures involving the fluorescent pyrene-labeled actin probe, to compare the interaction of the two proteins w...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00308a030
更新日期：1984-06-19 00:00:00
abstract：:Murine fibroblasts cultured in the presence of fibroblast growth factor-1 express relatively high levels of FR-1, a approximately 36 kDa protein related to the aldo-keto reductase superfamily [Donohue, P. J., Alberts, G. F., Hampton, B. S., Winkles, J. A. (1994) J. Biol. Chem. 269, 8604-8609]. While the crystal struct...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9804333
更新日期：1998-09-15 00:00:00
