RNA secondary structure: an important cis-element in rat calcitonin/CGRP pre-messenger RNA splicing.

abstract：:O6-Alkylguanine-DNA alkyltransferase (AGT) is an important cellular defense against the mutagenic effects of DNA alkylating agents. In humans this defense can contribute to the ability of some tumors to resist the effects of chemotherapeutic agents that act through DNA alkylation. We report here studies that character...

journal_title：Biochemistry

authors： Fried MG,Kanugula S,Bromberg JL,Pegg AE

更新日期：1996-12-03 00:00:00

abstract：:A combination of mutagenesis, calorimetry, kinetics, and small-angle X-ray scattering (SAXS) has been used to study the mechanism of ligand binding energy propagation through human cytochrome P450 reductase (CPR). Remarkably, the energetics of 2',5'-ADP binding to R597 at the FAD-binding domain are affected by mutatio...

journal_title：Biochemistry

authors： Grunau A,Geraki K,Grossmann JG,Gutierrez A

更新日期：2007-07-17 00:00:00

abstract：:Equilibrium binding experiments are widely used for the accurate characterization of binding and competitive binding behavior in biological systems. Modern high-throughput discovery efforts in chemical biology rely heavily upon this principle. Here, we derive exact analytical expressions for general competitive bindin...

journal_title：Biochemistry

authors： Roehrl MH,Wang JY,Wagner G

更新日期：2004-12-28 00:00:00

abstract：:In an effort to extend automated Edman degradation to nanomole quantities of protein, the method of sequenator analysis described by Edman and Begg (Edman, P., and Begg, G. (1967), Eur. J. Biochem. 1, 80) was modified to permit long degradations in the absence of carrier proteins. By using an aqueous 0.1 M Quadrol pro...

journal_title：Biochemistry

authors： Brauer AW,Margolies MN,Haber E

更新日期：1975-07-01 00:00:00

abstract：:The extrinsic 33-kDa protein (P33) was cross-linked covalently to the binding site on P33-depleted PSII preparations which is responsible for reconstitution of photosynthetic water oxidation after PSII preparations have been washed with 1 M CaCl2. Conditions were found in which more than half of the cross-linked prote...

journal_title：Biochemistry

authors： Mei R,Green JP,Sayre RT,Frasch WD

更新日期：1989-06-27 00:00:00

abstract：:Myelin basic protein (MBP) is a multifunctional protein involved in maintaining the stability and integrity of the myelin sheath by a variety of interactions with membranes and with cytoskeletal and other proteins. A central segment of MBP is highly conserved in mammals and consists of a membrane surface-associated am...

journal_title：Biochemistry

authors： Polverini E,Rangaraj G,Libich DS,Boggs JM,Harauz G

更新日期：2008-01-08 00:00:00

abstract：:Native calf thymus DNA was reacted with N-acetoxy-N-2-acetylaminofluorene (N-AcO-AAF) and its 7-fluoro and 7-iodo derivatives. Different ways of purification of the fluorene modified DNA samples were checked in order ot obtain a nucleic acid free from all noncovalently bound fluorene residues. The decrease in melting ...

journal_title：Biochemistry

authors： Fuchs RP,Lefevre JF,Pouyet J,Daune MP

更新日期：1976-07-27 00:00:00

abstract：:R67 dihydrofolate reductase (DHFR) is a novel homotetrameric protein that possesses 222 symmetry and a single, voluminous active site pore. This symmetry poses numerous limitations on catalysis; for example, two dihydrofolate (DHF) molecules or two NADPH molecules, or one substrate plus one cofactor can bind. Only the...

journal_title：Biochemistry

authors： Chopra S,Lynch R,Kim SH,Jackson M,Howell EE

更新日期：2006-05-30 00:00:00

abstract：:Acholeplasma laidlawii B cells, highly enriched in saturated C14 and C15 fatty acids, have been grown at several temperatures. Conformational order in the acyl chains of live cell membranes has been monitored with Fourier-transform infrared (FT-IR) spectroscopy. Coupled CH2 wagging progressions, characteristic of the ...

journal_title：Biochemistry

authors： Moore DJ,Mendelsohn R

更新日期：1994-04-05 00:00:00

abstract：:The actin depolymerizing factor (ADF)/cofilins are an essential group of proteins that are important regulators of actin filament turnover in vivo. Although protists and yeasts express only a single member of this family, metazoans express two or more members in many cell types. In cells expressing both ADF and cofili...

journal_title：Biochemistry

authors： Chen H,Bernstein BW,Sneider JM,Boyle JA,Minamide LS,Bamburg JR

更新日期：2004-06-08 00:00:00

abstract：:The human, cytosolic enzyme isocitrate dehydrogenase 1 (IDH1) reversibly converts isocitrate to α-ketoglutarate (αKG). Cancer-associated somatic mutations in IDH1 result in a loss of this normal function but a gain in a new or neomorphic ability to convert αKG to the oncometabolite 2-hydroxyglutarate (2HG). To improve...

journal_title：Biochemistry

authors： Rendina AR,Pietrak B,Smallwood A,Zhao H,Qi H,Quinn C,Adams ND,Concha N,Duraiswami C,Thrall SH,Sweitzer S,Schwartz B

更新日期：2013-07-02 00:00:00

abstract：:High-resolution proton nuclear magnetic resonance (1H NMR) measurements were made on myosin, heavy meromyosin (HMM), myosin subfragment 1 (S1), light meromyosin (LMM), and actin. A strong signal from amino acid side chains undergoing motions too fast to be accounted for by simple rotations of groups on a rigid backbon...

journal_title：Biochemistry

authors： Highsmith S,Akasaka K,Konrad M,Goody R,Holmes K,Wade-Jardetzky N,Jardetzky O

更新日期：1979-09-18 00:00:00

abstract：:We added phospholipase C-delta 1 (PLC-delta) to the aqueous subphase beneath monolayers formed from mixtures of phosphatidylinositol 4,5-bisphosphate (2% PIP2), phosphatidylserine (33% PS), and phosphatidylcholine (65% PC) and then measured the initial rate of hydrolysis of PIP2 after addition of 10 microM free calciu...

journal_title：Biochemistry

authors： Rebecchi M,Boguslavsky V,Boguslavsky L,McLaughlin S

更新日期：1992-12-29 00:00:00

abstract：:Dihydrofolate and dihydropteroylpolyglutamates inhibit pig liver methylenetetrahydrofolate reductase. In all cases the inhibition is linearly competitive with respect to methylenetetrahydrofolate. The Ki values decrease with each additional glutamyl residue from one to six, from a value of 6.5 microM for dihydrofolate...

journal_title：Biochemistry

authors： Matthews RG,Baugh CM

更新日期：1980-05-13 00:00:00

abstract：:The sigmoidal dependence of activity on substrate concentration exhibited by the regulatory enzyme aspartate transcarbamoylase (ATCase) of Escherichia coli is generally attributed to a ligand-promoted change in the quaternary structure of the enzyme. Although a global conformational change in ATCase upon the binding o...

journal_title：Biochemistry

authors： Eisenstein E,Markby DW,Schachman HK

更新日期：1990-04-17 00:00:00

abstract：:Binding of streptokinase (SK) to plasminogen (Pg) activates the zymogen conformationally and initiates its conversion into the fibrinolytic proteinase, plasmin (Pm). Equilibrium binding studies of SK interactions with a homologous series of catalytic site-labeled fluorescent Pg and Pm analogues were performed to resol...

journal_title：Biochemistry

authors： Boxrud PD,Bock PE

更新日期：2000-11-14 00:00:00

abstract：:Enzymic catalysis proceeds via intermediates formed in the course of substrate conversion. Here, we directly detect key intermediates in thiamin diphosphate (ThDP)-dependent enzymes during catalysis using (1)H NMR spectroscopy. The quantitative analysis of the relative intermediate concentrations allows the determinat...

journal_title：Biochemistry

authors： Tittmann K,Golbik R,Uhlemann K,Khailova L,Schneider G,Patel M,Jordan F,Chipman DM,Duggleby RG,Hübner G

更新日期：2003-07-08 00:00:00

abstract：:Upon injury of a blood vessel, activated factor VII (FVIIa) forms a high-affinity complex with its allosteric regulator, tissue factor (TF), and initiates blood clotting. Active site-inhibited factor VIIa (FVIIai) binds to TF with even higher affinity. We compared the interactions of FVIIai and FVIIa with soluble TF (...

journal_title：Biochemistry

authors： Osterlund M,Owenius R,Carlsson K,Carlsson U,Persson E,Lindgren M,Freskgård PO,Svensson M

更新日期：2001-08-07 00:00:00

abstract：:The steroidogenic acute regulatory protein (StAR), the first family member of START (StAR-related lipid transport) proteins, plays an essential role by facilitating the movement of cholesterol from the outer to inner mitochondrial membrane. Wild-type and mutant StAR binds cholesterol with similar intensity, but only w...

journal_title：Biochemistry

authors： Bose HS,Whittal RM,Bose M,Debnath D

更新日期：2009-02-17 00:00:00

abstract：:The fluorescence intensity of the two flavin prosthetic groups, FMN and FAD, in neuronal nitric oxide synthase (nNOS) was found to decay highly nonexponentially, being best described by four fluorescence lifetimes. This excited state heterogeneity is the result of multiple flavin quenching sites which are due to sever...

journal_title：Biochemistry

authors： Brunner K,Tortschanoff A,Hemmens B,Andrew PJ,Mayer B,Kungl AJ

更新日期：1998-12-15 00:00:00

abstract：:The conformational properties of an active-site loop segment, defined by residues Ser(161)-Glu(162)-Asn(163)-Ser(164), have been shown to be important for modulating the intrinsic reactivity of Mn(II) in the active site of Bacillus subtilis oxalate decarboxylase. We now detail the functional and structural consequence...

journal_title：Biochemistry

authors： Saylor BT,Reinhardt LA,Lu Z,Shukla MS,Nguyen L,Cleland WW,Angerhofer A,Allen KN,Richards NG

更新日期：2012-04-03 00:00:00

abstract：:The rate of deuterium exchange of a purine 8CH group in DNA is highly sensitive to both macromolecular secondary structure and intermolecular interactions which restrict solvent access to the major groove [Lamba, O.P., Becka, R., & Thomas, G.J., Jr. (1990) Biopolymers 29, 1465-1477]. We have exploited the sensitivity ...

journal_title：Biochemistry

authors： Reilly KE,Becka R,Thomas GJ Jr

更新日期：1992-03-31 00:00:00

abstract：:Translesion DNA synthesis represents the ability of a DNA polymerase to misinsert a nucleotide opposite a DNA lesion. Previous kinetic studies of the bacteriophage T4 DNA polymerase using a series of non-natural nucleotides suggest that pi-electron density of the incoming nucleotide substantially contributes to the ef...

journal_title：Biochemistry

authors： Zhang X,Donnelly A,Lee I,Berdis AJ

更新日期：2006-11-07 00:00:00

abstract：:The Na(+)-pumping NADH:quinone oxidoreductase (Na(+)-NQR) is a fundamental enzyme of the oxidative phosphorylation metabolism and ionic homeostasis in several pathogenic and marine bacteria. To understand the mechanism that couples electron transfer with sodium translocation in Na(+)-NQR, the ion dependence of the red...

journal_title：Biochemistry

authors： Neehaul Y,Juárez O,Barquera B,Hellwig P

更新日期：2012-05-15 00:00:00

abstract：:The 1.9 A X-ray crystal structure of human myeloperoxidase complexed with cyanide (R = 0.175, R(free) = 0.215) indicates that cyanide binds to the heme iron with a bent Fe-C-N angle of approximately 157 degrees, and binding is accompanied by movement of the iron atom by 0.2 A into the porphyrin plane. The bent orienta...

journal_title：Biochemistry

authors： Blair-Johnson M,Fiedler T,Fenna R

更新日期：2001-11-20 00:00:00

abstract：:A two-step pathway consisting of an acyl-acyl carrier protein (ACP) reductase (AAR) and an aldehyde-deformylating oxygenase (ADO) allows various cyanobacteria to convert long-chain fatty acids into hydrocarbons. AAR catalyzes the two-electron, NADPH-dependent reduction of a fatty acid attached to ACP via a thioester l...

journal_title：Biochemistry

authors： Warui DM,Pandelia ME,Rajakovich LJ,Krebs C,Bollinger JM Jr,Booker SJ

更新日期：2015-02-03 00:00:00

abstract：:M proteins and other members of the M protein family, expressed on the surface of Streptococcus pyogenes, bind host proteins such as immunoglobulins, albumin, and fibrinogen. Protein H and the M1 protein are expressed by adjacent genes and both belong to the M protein family. In this work, the structure and stability ...

journal_title：Biochemistry

authors： Nilson BH,Frick IM,Akesson P,Forsén S,Björck L,Akerström B,Wikström M

更新日期：1995-10-17 00:00:00

abstract：:We have investigated some properties related to interaction with DNA and recognition of AT-rich sequences of netropsin-oxazolopyridocarbazole (Net-OPC) (Mrani et al., 1990), which is a hybrid groove-binder-intercalator. The hybrid molecule Net-OPC binds to poly[d(A-T)] at two different sites with Kapp values close to ...

journal_title：Biochemistry

authors： Subra F,Carteau S,Pager J,Paoletti J,Paoletti C,Auclair C,Mrani D,Gosselin G,Imbach JL

更新日期：1991-02-12 00:00:00

abstract：:The reaction of cytochrome c oxidase with hydrogen peroxide has been of great value in generating and characterizing oxygenated species of the enzyme that are identical or similar to those formed during turnover of the enzyme with dioxygen. Most previous studies have utilized relatively low peroxide concentrations (mi...

journal_title：Biochemistry

authors： Zaslavsky D,Smirnova IA,Brzezinski P,Shinzawa-Itoh K,Yoshikawa S,Gennis RB

更新日期：1999-11-30 00:00:00

abstract：:Cytochrome P450BM3 is a bacterial enzyme with a heme cofactor that binds small diatomic ligands. Here we report the first study of carbon monoxide (CO) photodissociation and rebinding in ferrous P450BM3 on an ultrafast time scale. We monitored dissociation of carbon monoxide upon Soret band excitation using visible an...

journal_title：Biochemistry

authors： Rupenyan A,Commandeur J,Groot ML

更新日期：2009-07-07 00:00:00