Synthesis of 3'-C-methyladenosine and 3'-C-methyluridine diphosphates and their interaction with the ribonucleoside diphosphate reductase from Corynebacterium nephridii.

abstract：:The post-polyketide synthase (PKS) biosynthetic tailoring of macrolide antibiotics usually involves one or more oxidation reactions catalyzed by cytochrome P450 monooxygenases. As the specificities of members from this class of enzymes vary significantly among PKS gene clusters, the identification and study of new mac...

journal_title：Biochemistry

authors： Betlach MC,Kealey JT,Ashley GW,McDaniel R

更新日期：1998-10-20 00:00:00

abstract：:Light chain amyloidoses arise from the overproduction and abnormal deposition of the immunoglobulin light chain in various organs. LEN is the variable domain of an immunoglobulin light chain originally isolated from the urine of a patient suffering from multiple myeloma, with no sign of renal dysfunction or amyloidosi...

journal_title：Biochemistry

authors： Hu D,Qin Z,Xue B,Fink AL,Uversky VN

更新日期：2008-08-19 00:00:00

abstract：:In an effort to characterize the bimolecular interface between parathyroid hormone (PTH) and its human receptor PTH1-Rc (hPTH1-Rc), we previously identified two contact sites in the receptor: one for position 1 and another for position 13 (located at the ends of the principal activation domain) in PTH(1-34). The prese...

journal_title：Biochemistry

authors： Greenberg Z,Bisello A,Mierke DF,Rosenblatt M,Chorev M

更新日期：2000-07-18 00:00:00

abstract：:Choline oxidase catalyzes the oxidation of choline to glycine betaine through a two-step, four-electron reaction with betaine aldehyde as an intermediate. Oxygen is the final electron acceptor. Alcohol oxidation is initiated by the removal of the substrate hydroxyl proton by an unknown active site residue with a pKa v...

journal_title：Biochemistry

authors： Smitherman C,Rungsrisuriyachai K,Germann MW,Gadda G

更新日期：2015-01-20 00:00:00

abstract：:A soluble and fully functional 10.5 kDa fragment of the 18.2 kDa membrane-bound cytochrome c(552) from Paracoccus denitrificans has been heterologously expressed and (13)C/(15)N-labeled to study the structural features of this protein in both redox states. Well-resolved solution structures of both the reduced and oxid...

journal_title：Biochemistry

authors： Reincke B,Pérez C,Pristovsek P,Lücke C,Ludwig C,Löhr F,Rogov VV,Ludwig B,Rüterjans H

更新日期：2001-10-16 00:00:00

abstract：:Two hybridomas producing monoclonal antibodies to poly(adenosine diphosphate ribose) [poly(ADP-Rib)] were established. One antibody, 10H (IgG3, kappa), bound to most of the poly(ADP-Rib) preparation, which consisted of molecules of various sizes of more than 20 ADP-Rib residues. The binding of this antibody was inhibi...

journal_title：Biochemistry

authors： Kawamitsu H,Hoshino H,Okada H,Miwa M,Momoi H,Sugimura T

更新日期：1984-07-31 00:00:00

abstract：:The blood-clotting protein fibrinogen is composed of three subunits, designated A alpha, B beta, and gamma, which are encoded by a family of related genes. As part of the acute-phase response, expression of the fibrinogen genes is coordinately regulated in the liver by glucocorticoids. To understand the factors underl...

journal_title：Biochemistry

authors： Roberts LR,Nichols LA,Holland LJ

更新日期：1993-11-02 00:00:00

abstract：:Although Mg(2+) is the cation that functions as the cofactor for the nucleotidyl transfer reaction for almost all DNA polymerases, Mn(2+) can also serve, but when it does, the degree of base discrimination exhibited by most DNA polymerases (pols) is diminished. Metal ions other than Mg(2+) or Mn(2+) can also act as co...

journal_title：Biochemistry

authors： Vashishtha AK,Konigsberg WH

更新日期：2016-05-10 00:00:00

abstract：:In the cyanobacterium Anabaena PCC7120, the precursor to tRNA(Leu) contains a 249-nucleotide group I intron that undergoes efficient self-splicing in vitro. By deleting the 5' and 3' splice sites, this intron has now been converted to an RNA enzyme that uses a guanosine nucleophile to cleave substrate RNAs (S) with mu...

journal_title：Biochemistry

authors： Zaug AJ,Dávila-Aponte JA,Cech TR

更新日期：1994-12-13 00:00:00

abstract：:The characterization of cod somatolactin (SL), a new pituitary protein belonging to the growth hormone/prolactin family, is described. Cod SL has a molecular weight of 26 kDa and consists of 209 amino acids, of which eight are Cys. The protein has three disulfide bonds between residues Cys5-Cys15, Cys65-Cys181, and Cy...

journal_title：Biochemistry

authors： Rand-Weaver M,Noso T,Muramoto K,Kawauchi H

更新日期：1991-02-12 00:00:00

abstract：:The initiation of Escherichia coli chromosomal replication by DnaA protein is strongly influenced by the tight binding of the nucleotides ATP and ADP. Anionic phospholipids in a fluid bilayer promote the conversion of inactive ADP-DnaA protein to replicatively active ATP-DnaA protein in vitro, and thus likely play a k...

journal_title：Biochemistry

authors： Kitchen JL,Li Z,Crooke E

更新日期：1999-05-11 00:00:00

abstract：:Mycoplasmas are the smallest known microorganisms, with drastically reduced genome sizes. One of the essential biochemical pathways lost in mycoplasmas is methylation-mediated DNA repair (MMR), which is responsible for correction of base substitutions, insertions, and deletions in both bacteria and higher organisms. W...

journal_title：Biochemistry

authors： Kamashev D,Oberto J,Serebryakova M,Gorbachev A,Zhukova Y,Levitskii S,Mazur AK,Govorun V

更新日期：2011-10-11 00:00:00

abstract：:We reacted uracil-containing M13mp2 DNA with N-hydroxy-2-aminofluorene to produce a template with N-(deoxyguanosin-8-yl)-2-aminofluorene adducts. This template was hybridized to a non-uracil-containing linear fragment from which the lac z complementing insert had been removed to produce a gapped substrate. DNA synthes...

journal_title：Biochemistry

authors： Sahm J,Turkington E,LaPointe D,Strauss B

更新日期：1989-04-04 00:00:00

abstract：:The effect of concentrated glycerol on the thermal transitions of chymotrypsinogen and ribonuclease has been examined by differential spectrophotometry at 293 and 287 mm, respectively. It was found that for both proteins addition of glycerol raises the transition temperature, the increase in Tm being greater for ribon...

journal_title：Biochemistry

authors： Gekko K,Timasheff SN

更新日期：1981-08-04 00:00:00

abstract：:Three electrochromic difference spectra induced by the deposition of (1) a negative charge on the primary quinone acceptor, Q(A), (2) a positive charge on (or near) Tyr161 of the D1 subunit (Y(Z)), and (3) a positive charge on the manganese cluster were determined at room temperature in photosystem II (PSII) core part...

journal_title：Biochemistry

authors： Mulkidjanian AY,Cherepanov DA,Haumann M,Junge W

更新日期：1996-03-05 00:00:00

abstract：:A variant of the Tetrahymena ribozyme that efficiently cleaves single-stranded DNA under simulated physiological conditions [Tsang, J., & Joyce, G. F. (1994) Biochemistry 33, 5966-5973] was evaluated as a potential therapeutic agent on the basis of its ability to cleave synthetic oligonucleotide substrates correspondi...

journal_title：Biochemistry

authors： Raillard SA,Joyce GF

更新日期：1996-09-10 00:00:00

abstract：:The crystal structures of metyrapone- and 1-, 2-, and 4-phenylimidazole-inhibited complexes of cytochrome P-450cam have been refined to a nominal resolution of 2.1 A and compared with the 1.63-A camphor-bound structure. With the exception of 2-phenylimidazole, each of the inhibitors forms an N-Fe bond with the heme ir...

journal_title：Biochemistry

authors： Poulos TL,Howard AJ

更新日期：1987-12-15 00:00:00

abstract：:Integrin αIIbβ3, a transmembrane heterodimer, mediates platelet aggregation when it switches from an inactive to an active ligand-binding conformation following platelet stimulation. Central to regulating αIIbβ3 activity is the interaction between the αIIb and β3 extracellular stalks, which form a tight heterodimer in...

journal_title：Biochemistry

authors： Tan SK,Fong KP,Polizzi NF,Sternisha A,Slusky JSG,Yoon K,DeGrado WF,Bennett JS

更新日期：2019-07-30 00:00:00

abstract：:1,4-Dihydroxy-2-naphthoyl-coenzyme A (DHNA-CoA) synthase, or MenB, catalyzes an intramolecular Claisen condensation involving two oxyanion intermediates in the biosynthetic pathway of menaquinone, an essential respiration electron transporter in many microorganisms. Here we report the finding that the DHNA-CoA product...

journal_title：Biochemistry

authors： Chen M,Jiang M,Sun Y,Guo ZF,Guo Z

更新日期：2011-07-05 00:00:00

abstract：:This report describes the determination of the complete primary structure of the adhesin receptor polysaccharide of Streptococcus oralis ATCC 55229 (previously characterized as Streptococcus sanguis H1), a Gram-positive bacteria implicated in dental plaque formation. The polysaccharide was isolated from S. oralis ATCC...

journal_title：Biochemistry

authors： Glushka J,Cassels FJ,Carlson RW,van Halbeek H

更新日期：1992-11-10 00:00:00

abstract：:A modified multiple binding equilibria treatment is presented that allows determination of thermodynamic parameters of the interaction of phospholipids with integral membrane proteins solubilized in excess detergent. Lipid binding is modeled as a series of exchange reactions between lipid molecules and detergent molec...

journal_title：Biochemistry

authors： Volwerk JJ,Mrsny RJ,Patapoff TW,Jost PC,Griffith OH

更新日期：1987-01-27 00:00:00

abstract：:Protein-protein interactions mediate the vast majority of cellular processes. Though protein interactions obey basic chemical principles also within the cell, the in vivo physiological environment may not allow for equilibrium to be reached. Thus, in vitro measured thermodynamic affinity may not provide a complete pic...

journal_title：Biochemistry

authors： Cohen-Khait R,Schreiber G

更新日期：2018-08-07 00:00:00

abstract：:Fatty acid-induced upregulation of death receptor 5 (DR5) and its cognate ligand, tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), promotes hepatocyte lipoapoptosis, which is a key mechanism in the progression of fatty liver disease. Accordingly, inhibition of DR5 signaling represents an attractive str...

journal_title：Biochemistry

authors： Vunnam N,Szymonski S,Hirsova P,Gores GJ,Sachs JN,Hackel BJ

更新日期：2020-10-13 00:00:00

abstract：:Aromatic amino acids often flank the transmembrane alpha helices of integral membrane proteins. By favoring locations within the membrane-water interface of the lipid bilayer, aromatic residues Trp, Tyr, and sometimes Phe may serve as anchors to help stabilize a transmembrane orientation. In this work, we compare the ...

journal_title：Biochemistry

authors： Sparks KA,Gleason NJ,Gist R,Langston R,Greathouse DV,Koeppe RE 2nd

更新日期：2014-06-10 00:00:00

abstract：:The light-induced global conformational change of photoactive yellow protein was directly observed by small-angle X-ray scattering (SAXS). The N-terminal 6, 15, or 23 amino acid residues were enzymatically truncated (T6, T15, or T23, respectively), and their near-UV intermediates were accumulated under continuous illu...

journal_title：Biochemistry

authors： Imamoto Y,Kamikubo H,Harigai M,Shimizu N,Kataoka M

更新日期：2002-11-19 00:00:00

abstract：:Herpesvirus saimiri codes for a tyrosine kinase interacting protein (Tip) that interacts with both the SH3 domain and the kinase domain of the T-cell-specific tyrosine kinase Lck via two separate motifs. The activation of Lck by Tip is considered as a key event in the transformation of human T-lymphocytes during herpe...

journal_title：Biochemistry

authors： Schweimer K,Hoffmann S,Bauer F,Friedrich U,Kardinal C,Feller SM,Biesinger B,Sticht H

更新日期：2002-04-23 00:00:00

abstract：:In the native calcium-binding protein calbindin D9k (M(r) 8.700; 75aa; 2 EF-hands), the backbone carbonyl oxygen of Glu60 coordinates the Ca2+ ion in the C-terminal site (site II). The carboxylate group of the same residue forms a hydrogen bond to a water molecule that constitutes a Ca2+ ligand in the N-terminal site ...

journal_title：Biochemistry

authors： Linse S,Bylsma NR,Drakenberg T,Sellers P,Forsén S,Thulin E,Svensson LA,Zajtzeva I,Zajtsev V,Marek J

更新日期：1994-10-18 00:00:00

abstract：:R67 dihydrofolate reductase (DHFR), which catalyzes the NADPH dependent reduction of dihydrofolate to tetrahydrofolate, belongs to a type II family of R-plasmid encoded DHFRs that confer resistance to the antibacterial drug trimethoprim. Crystal structure data reveals this enzyme is a homotetramer that possesses a sin...

journal_title：Biochemistry

authors： Hicks SN,Smiley RD,Hamilton JB,Howell EE

更新日期：2003-09-16 00:00:00

abstract：:Apamin is being studied as a model for the folding mechanism of proteins whose structures are stabilized by disulfide bonds. Apamin consists of 18 amino acid residues and forms a stable structure consisting of a C-terminal alpha-helix and two reverse turns. This structure is stabilized by two disulfide bonds connectin...

journal_title：Biochemistry

authors： Chau MH,Nelson JW

更新日期：1992-05-12 00:00:00

abstract：:Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a glycolytic enzyme that also functions in transcriptional regulation, oxidative stress, vesicular trafficking, and apoptosis. Because GAPDH is required for the insertion of cellular heme into inducible nitric oxide synthase [Chakravarti, R., et al. (2010) Proc. Natl...

journal_title：Biochemistry

authors： Hannibal L,Collins D,Brassard J,Chakravarti R,Vempati R,Dorlet P,Santolini J,Dawson JH,Stuehr DJ

更新日期：2012-10-30 00:00:00