Three small ribooligonucleotides with specific arginine sites.

abstract：:The reactions of fully reduced and mixed-valence bovine heart cytochrome c oxidase with dioxygen have been reinvestigated in the absence and presence of metal ions (Zn(2+), Ni(2+), and Cd(2+)) by time-resolved optical absorption spectroscopy using the CO flow-flash technique. The time-resolved data were recorded on a ...

journal_title：Biochemistry

authors： Szundi I,Cappuccio J,Einarsdóttir O

更新日期：2004-12-21 00:00:00

abstract：:Photoaffinity labeling of the nicotinic acetylcholine receptor from Torpedo marmorata electric tissue was performed in the presence of cholinergic effectors in the millisecond to second time range by a combination of a stopped-flow apparatus and a high-energy pulse laser. The label applied was [3H]triphenylmethylphosp...

journal_title：Biochemistry

authors： Muhn P,Fahr A,Hucho F

更新日期：1984-06-05 00:00:00

abstract：:We have investigated the effects of mixed Na+: Mg2+ ionic solutions on the stability of the nonspecific lac repressor-DNA complex. The effects of Mg2+ are simply interpreted in terms of its role as a competitor (with repressor) for DNA sites. From these studies, the binding constant of the Mg-DNA complex can be determ...

journal_title：Biochemistry

authors： Record MT Jr,deHaseth PL,Lohman TM

更新日期：1977-11-01 00:00:00

abstract：:The pivotal role of the Arg-Gly-Asp (RGD) peptide motif in integrin-mediated cell adhesive interactions with extracellular matrix and plasma proteins stimulated the present design of nonpeptidic mimetics of this sequence. To probe the structural requirements for RGD recognition by integrins, we designed various struct...

journal_title：Biochemistry

authors： Greenspoon N,Hershkoviz R,Alon R,Varon D,Shenkman B,Marx G,Federman S,Kapustina G,Lider O

更新日期：1993-02-02 00:00:00

abstract：:Mercuric reductase, a flavoprotein disulfide oxidoreductase, catalyzes the two-electron reduction of Hg(II) to Hg(0) by NADPH. As with all the members of this class of proteins, the enzyme is a dimer of identical subunits with two active sites per dimer, each composed of one FAD and catalytically essential residues fr...

journal_title：Biochemistry

authors： Miller SM,Massey V,Williams CH Jr,Ballou DP,Walsh CT

更新日期：1991-03-12 00:00:00

abstract：:A simple and rapid procedure is described for purification of P-glycoprotein (Pgp) from a multidrug-resistant Chinese hamster ovary cell line (CR1R12) in which the plasma membranes are highly enriched in Pgp (Al-Shawi, M.K., Senior A.E. (1993) J. Biol. Chem, 268, 4197-4206). The procedure consisted of octylglucoside s...

journal_title：Biochemistry

authors： Urbatsch IL,al-Shawi MK,Senior AE

更新日期：1994-06-14 00:00:00

abstract：:The inheritance of DNA methylation patterns may play an important role in the stability of the differentiated state. We have therefore studied the inhibitory effects of DNA binding proteins on DNA methylation in vitro. Mouse L1210 cells grown in the presence of 5-azacytidine acquire hemimethylated sites in their DNA. ...

journal_title：Biochemistry

authors： Kautiainen TL,Jones PA

更新日期：1985-02-26 00:00:00

abstract：:Geobacillus stearothermophilus T-6 encodes for a beta-xylosidase (XynB2) from family 52 of glycoside hydrolases that was previously shown to hydrolyze its substrate with net retention of the anomeric configuration. XynB2 significantly prefers substrates with xylose as the glycone moiety and exhibits a typical bell-sha...

journal_title：Biochemistry

authors： Bravman T,Zolotnitsky G,Belakhov V,Shoham G,Henrissat B,Baasov T,Shoham Y

更新日期：2003-09-09 00:00:00

abstract：:In the cytochrome P450cam-dependent monooxygenase system from Pseudomonas putida, putidaredoxin (Pdx) shuttles electrons between putidaredoxin reductase (Pdr) and P450cam and, thus, must form transient complexes with both partners. 1-Ethyl 3-[3-(dimethylamino)propyl]carbodiimide (EDC) was found to promote formation of...

journal_title：Biochemistry

authors： Churbanova IY,Poulos TL,Sevrioukova IF

更新日期：2010-01-12 00:00:00

abstract：:N-[[(Iodoacetyl)amino]ethyl]-5-naphthylamine-1-sulfonic acid (IAEDANS) is a fluorescent reagent which reacts covalently with the free thiol groups of proteins. When the reagent is reacted with the Escherichia coli ribosome under mild conditions, gel electrophoresis shows modification of predominantly two proteins, S18...

journal_title：Biochemistry

authors： Hanas JS,Simpson MV

更新日期：1985-12-03 00:00:00

abstract：:We have characterized by EPR the interaction of the Em,7 = -50 mV [4Fe-4S] cluster of Escherichia coli DMSO reductase (DmsABC) with a menaquinol (MQH2) binding site. Potentiometric titrations indicate that in DmsAB(C102S)C, the Em,7 = -50 mV [4Fe-4S] cluster is replaced by an Em,7 = +260 mV [3Fe-4S] cluster. The Q-poo...

journal_title：Biochemistry

authors： Rothery RA,Weiner JH

更新日期：1996-03-12 00:00:00

abstract：:High hydrostatic pressure has been shown to cause reversible dissociation of the isolated apo beta 2 dimer of tryptophan synthase from Escherichia coli into enzymatically inactive monomers [Seifert, T., Bartholmes, P., & Jaenicke, R. (1982) Biophys. Chem. 15, 1-8]. Addition of the coenzyme pyridoxal 5'-phosphate affec...

journal_title：Biochemistry

authors： Seifert T,Bartholmes P,Jaenicke R

更新日期：1985-01-15 00:00:00

abstract：:A human genomic DNA library was screened for the gene coding for the gamma chain of fibrinogen by using a human cDNA for the gamma chain as a hybridization probe. The gene was identified in three overlapping recombinant lambda bacteriophage, and its sequence, including the immediate 5' and 3' flanking regions, was det...

journal_title：Biochemistry

authors： Rixon MW,Chung DW,Davie EW

更新日期：1985-04-09 00:00:00

abstract：:Previously we showed that the low-molecular-weight mucin (MG2, encoded by MUC7), a major component of human submandibular/sublingual saliva, is a bacterial receptor that coats the tooth surface. Here we tested the hypothesis that the structure of its carbohydrate residues contains important information about its funct...

journal_title：Biochemistry

authors： Prakobphol A,Thomsson KA,Hansson GC,Rosen SD,Singer MS,Phillips NJ,Medzihradszky KF,Burlingame AL,Leffler H,Fisher SJ

更新日期：1998-04-07 00:00:00

abstract：:Photolyzed rhodopsin is phosphorylated at multiple serine and threonine residues during the quenching of phototransduction. Sites of phosphorylation by rhodopsin kinase have been localized to the C-terminal region of rhodopsin, but no information was available on the kinetics and identity of phosphorylated residues. T...

journal_title：Biochemistry

authors： Ohguro H,Palczewski K,Ericsson LH,Walsh KA,Johnson RS

更新日期：1993-06-01 00:00:00

abstract：:Type I repeat sequences are evolutionarily conserved sequence elements found in the replication origin and transcriptional promoter region of the rRNA genes (rDNA) in Tetrahymena thermophila. An abundant single-stranded DNA binding protein, ssA-TIBF, specifically interacts with the A-rich strand of the Type I repeat s...

journal_title：Biochemistry

authors： Hou Z,Umthun AR,Dobbs DL

更新日期：1995-04-11 00:00:00

abstract：:Calcium binding to triads isolated from rabbit skeletal muscle followed a single hyperbolic function in the pH range 5.5-8.0. Maximal binding was obtained at pH 8.0; decreasing the pH decreased the binding capacity and, at pH < or = 6.0, increased Kd 2-fold. These results indicate that lowering the pH diminished calci...

journal_title：Biochemistry

authors： Donoso P,Beltrán M,Hidalgo C

更新日期：1996-10-15 00:00:00

abstract：:There is much debate whether the fatty acid substrate of lipoxygenase binds "carboxylate-end first" or "methyl-end first" in the active site of soybean lipoxygenase-1 (sLO-1). To address this issue, we investigated the sLO-1 mutants Trp500Leu, Trp500Phe, Lys260Leu, and Arg707Leu with steady-state and stopped-flow kine...

journal_title：Biochemistry

authors： Ruddat VC,Mogul R,Chorny I,Chen C,Perrin N,Whitman S,Kenyon V,Jacobson MP,Bernasconi CF,Holman TR

更新日期：2004-10-19 00:00:00

abstract：:The recruitment and organization of clathrin at endocytic sites first to form coated pits and then clathrin-coated vesicles depend on interactions between the clathrin N-terminal domain (TD) and multiple clathrin binding sequences on the cargo adaptor and accessory proteins that are concentrated at such sites. Up to f...

journal_title：Biochemistry

authors： Zhuo Y,Cano KE,Wang L,Ilangovan U,Hinck AP,Sousa R,Lafer EM

更新日期：2015-04-28 00:00:00

abstract：:Macrophage migration inhibitory factor (MIF) is a pro-inflammatory and tumor-promoting cytokine that occurs in two redox-dependent immunologically distinct conformational isoforms. The disease-related structural isoform of MIF (oxMIF) can be specifically and predominantly detected in the circulation of patients with i...

journal_title：Biochemistry

authors： Schinagl A,Kerschbaumer RJ,Sabarth N,Douillard P,Scholz P,Voelkel D,Hollerweger JC,Goettig P,Brandstetter H,Scheiflinger F,Thiele M

更新日期：2018-03-06 00:00:00

abstract：:The interaction between hemoglobin and the cytoplasmic surface of human erythrocyte membranes at physiological pH was studied by monitoring the electron paramagnetic resonance (EPR) signal of spin-labeled membrane ghosts in hemoglobin solutions of various concentrations. The EPR spectra indicate the existence of a sig...

journal_title：Biochemistry

authors： Fung LW

更新日期：1981-12-08 00:00:00

abstract：:Human secretory group IIa phospholipase A2 (hIIa-PLA2) contains a large number of prominent cationic patches on its molecular surface and has exceptionally high affinity for anionic surfaces, including anionic membranes. To identify the cationic amino acid residues that support binding of hIIa-PLA2 to anionic membrane...

journal_title：Biochemistry

authors： Snitko Y,Koduri RS,Han SK,Othman R,Baker SF,Molini BJ,Wilton DC,Gelb MH,Cho W

更新日期：1997-11-25 00:00:00

abstract：:Z-disc-associated, alternatively spliced, PDZ motif-containing protein (ZASP) is a principal component of the sarcomere. The three prevalent isoforms of ZASP in skeletal muscle are generated by alternative splicing of exons 9 and 10. The long isoforms, either having (ZASP-L) or lacking exon 10 (ZASP-LΔex10), include a...

journal_title：Biochemistry

authors： Watts NR,Zhuang X,Kaufman JD,Palmer IW,Dearborn AD,Coscia S,Blech-Hermoni Y,Alfano C,Pastore A,Mankodi A,Wingfield PT

更新日期：2017-04-11 00:00:00

abstract：:The thermodynamics of L-tryptophan and operator DNA binding to the tryptophan repressor of Escherichia coli were analyzed by titration microcalorimetry and van't Hoff analysis of footprinting titrations, respectively. At 25 degrees C in 10 mM sodium phosphate, pH 7.6, and 0.1 M NaCl, the binding of L-tryptophan to the...

journal_title：Biochemistry

authors： Jin L,Yang J,Carey J

更新日期：1993-07-20 00:00:00

abstract：:The protease activity of hepatitis C virus nonstructural protein 3 (NS3) is essential for viral replication. ITMN-191, a macrocyclic inhibitor of the NS3 protease active site, promotes rapid, multilog viral load reductions in chronic HCV patients. Here, ITMN-191 is shown to be a potent inhibitor of NS3 with a two-step...

journal_title：Biochemistry

authors： Rajagopalan R,Misialek S,Stevens SK,Myszka DG,Brandhuber BJ,Ballard JA,Andrews SW,Seiwert SD,Kossen K

更新日期：2009-03-24 00:00:00

abstract：:The human endonuclease III homologue (hNTH1) removes premutagenic cytosine damage from DNA. This includes 5-hydroxycytosine, which has increased potential for pairing with adenine, resulting in C --> T transition mutations. Here we report that hNTH1 acts on both 5-hydroxycytosine and abasic sites preferentially when t...

journal_title：Biochemistry

authors： Eide L,Luna L,Gustad EC,Henderson PT,Essigmann JM,Demple B,Seeberg E

更新日期：2001-06-05 00:00:00

abstract：:The chemical mechanism of the phosphoribosyltransferases (PRTases), although largely unknown, may proceed either via a concerted direct-transfer mechanism or with a two-step mechanism involving a carboxonium-like intermediate. To study this question, we have cloned the Salmonella typhimurium pyrE gene, coding for the ...

journal_title：Biochemistry

authors： Bhatia MB,Vinitsky A,Grubmeyer C

更新日期：1990-11-20 00:00:00

abstract：:A novel truncated form (residues 1-214, with a randomized C-terminal tail) of the ligand-binding extracellular domain (ECD) of the human alpha1 glycine receptor (GlyR), with amino acids from the corresponding sequence of an acetylcholine binding protein (AChBP) substituted for two relatively hydrophobic membrane-proxi...

journal_title：Biochemistry

authors： Liu Z,Ramanoudjame G,Liu D,Fox RO,Jayaraman V,Kurnikova M,Cascio M

更新日期：2008-09-16 00:00:00

abstract：:High-resolution phosphorus-31 nuclear magnetic resonance (31P NMR) spectra of wild-type and mutant strains of Saccharomyces cerevisiae were observed at a frequency of 145.7 MHz. Levels of various phosphorus metabolites were investigated upon addition of glucose under both aerobic and anaerobic conditions. Three mutant...

journal_title：Biochemistry

authors： Navon G,Shulman RG,Yamane T,Eccleshall TR,Lam KB,Baronofsky JJ,Marmur J

更新日期：1979-10-16 00:00:00

abstract：:The heme-regulated inhibitor (HRI) of protein synthesis becomes activated in rabbit reticulocyte lysates in response to a variety of conditions including heme-deficiency, addition of oxidants, and heat shock. Activated HRI inhibits translation by catalyzing the phosphorylation of the alpha-subunit of eukaryotic initia...

journal_title：Biochemistry

authors： Matts RL,Hurst R,Xu Z

更新日期：1993-07-27 00:00:00