Tryptophan 500 and arginine 707 define product and substrate active site binding in soybean lipoxygenase-1.

abstract：:Quasi-elastic laser light scattering is used to examine the cholera toxin mediated agglutination of ganglioside GM1 containing phospholipid vesicles and Gm1-coated polystyrene spheres. We find that the ability of cholera toxin to agglutinate Gm1-containing phospholipid vesicles depends markedly on the lipid compositio...

journal_title：Biochemistry

authors： Dwyer JD,Bloomfield VA

更新日期：1982-06-22 00:00:00

abstract：:The structure of the ca. 250 amino acid hormone binding domain of the human estrogen receptor (hER-LBD), expressed in E. coli and purified as a complex with estradiol, has been probed by selective proteolysis, with analysis of the protein fragments both by classical methods (SDS-PAGE and Edman N-terminal sequencing) a...

journal_title：Biochemistry

authors： Seielstad DA,Carlson KE,Kushner PJ,Greene GL,Katzenellenbogen JA

更新日期：1995-10-03 00:00:00

abstract：:A major regulatory step in the heat-induced transcription of heat shock protein (hsp) genes in eukaryotes is the activation of heat shock factor (HSF). In metazoans and Schizosaccharomyces pombe, HSF is present in unstressed cells but is unable to bind to its target DNA sequence element, the heat shock element (HSE). ...

journal_title：Biochemistry

authors： Larson JS,Schuetz TJ,Kingston RE

更新日期：1995-02-14 00:00:00

abstract：:Escherichia coli 16S RNA from 30S ribosomal subunits was isolated, oxidized at the 3' end, and labeled with the thiosemicarbazide derivatives of fluorescein or eosin. Labeled 16S RNA was reconstituted into 30S subunits. They were almost fully active compared to 30S subunits reconstituted from unlabeled 16S RNA by usin...

journal_title：Biochemistry

authors： Robbins D,Odom OW Jr,Lynch J,Kramer G,Hardesty B,Liou R,Ofengand J

更新日期：1981-09-01 00:00:00

abstract：:Serine transacetylase from Salmonella typhimurium was purified to near homogeneity. A complete initial velocity study in both reaction directions suggests that the enzyme catalyzes the conversion of acetyl CoA and L-serine to O-acetyl-L-serine (OAS) and coenzyme A (CoASH) by a ping pong bi bi kinetic mechanism. Initia...

journal_title：Biochemistry

authors： Leu LS,Cook PF

更新日期：1994-03-08 00:00:00

abstract：:The human immunodeficiency virus utilizes its gp41 fusion protein to mediate virus-cell membrane fusion. The conserved disulfide loop region in the gp41 hairpin conformation reverses the protein chain, such that the N-terminal heptad repeat and the C-terminal heptad repeat regions interact to form the six-helix bundle...

journal_title：Biochemistry

authors： Ashkenazi A,Merklinger E,Shai Y

更新日期：2012-09-04 00:00:00

abstract：:The l-lyxonate dehydratase (LyxD) in vitro enzymatic activity and in vivo metabolic function were assigned to members of an isofunctional family within the mandelate racemase (MR) subgroup of the enolase superfamily. This study combined in vitro and in vivo data to confirm that the dehydration of l-lyxonate is the bio...

journal_title：Biochemistry

authors： Ghasempur S,Eswaramoorthy S,Hillerich BS,Seidel RD,Swaminathan S,Almo SC,Gerlt JA

更新日期：2014-05-27 00:00:00

abstract：:It was previously observed that two steps of the reaction of eukaryotic DNA topoisomerase I (topoisomerization and cleavage) depend upon the conformation of the DNA substrate: in both instances the supercoiled form is a more efficient substrate than the relaxed one. This paper reports the analysis of two other steps o...

journal_title：Biochemistry

authors： Caserta M,Amadei A,Camilloni G,Di Mauro E

更新日期：1990-09-04 00:00:00

abstract：:The cytochrome aa3-type terminal quinol oxidase of Bacillus subtilis catalyzes the four-electron reduction of dioxygen to water. It resembles the aa3-type cytochrome-c oxidase in using heme A as its active-site chromophores but lacks the CuA center and the cytochrome-c oxidizing activity of the mitochondrial enzyme. W...

journal_title：Biochemistry

authors： Lauraeus M,Wikström M,Varotsis C,Tecklenburg MM,Babcock GT

更新日期：1992-10-20 00:00:00

abstract：:Formylglycinamide ribonucleotide amidotransferase (FGAR-AT) catalyzes the ATP- and glutamine-dependent formation of formylglycinamidine ribonucleotide, ADP, P(i), and glutamate in the fourth step of de novo purine biosynthesis. Like all amidotransferases (ATs), FGAR-AT is proposed to channel ammonia between a glutamin...

journal_title：Biochemistry

authors： Hoskins AA,Anand R,Ealick SE,Stubbe J

更新日期：2004-08-17 00:00:00

abstract：:A new strategy for the assignment of nonexchangeable proton resonances in oligonucleotide duplexes is presented and used to interpret the spectra of the oligonucleotide helix d(CpGpCpGpCpG) X d(CpGpCpGpCpG) in low salt (B form). This procedure is based on the use of sequential homodecoupling (1D) or COSY (2D) for the ...

journal_title：Biochemistry

authors： Frechet D,Cheng DM,Kan LS,Ts'o PO

更新日期：1983-10-25 00:00:00

abstract：:Venomous organisms have evolved a variety of structurally diverse peptide neurotoxins that target ion channels. Despite the lack of any obvious structural homology, unrelated toxins that interact with voltage-activated K(+) channels share a dyad motif composed of a lysine and a hydrophobic amino acid residue, usually ...

journal_title：Biochemistry

authors： Al-Sabi A,Lennartz D,Ferber M,Gulyas J,Rivier JE,Olivera BM,Carlomagno T,Terlau H

更新日期：2004-07-13 00:00:00

abstract：:Prion diseases are associated with the conversion of the normal prion protein, PrP(C), to the infectious disease form PrP(Sc). Discrimination between these isoforms would significantly enhance diagnosis of these diseases, and it has recently been reported that PrP(Sc) is specifically recognized by the serine protease ...

journal_title：Biochemistry

authors： Ellis V,Daniels M,Misra R,Brown DR

更新日期：2002-06-04 00:00:00

abstract：:In the anaerobic sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough (DvH), the genome sequencing revealed the presence of three operons encoding formate dehydrogenases. fdh1 encodes an alphabetagamma trimeric enzyme containing 11 heme binding sites; fdh2 corresponds to an alphabetagamma trimeric enzyme wi...

journal_title：Biochemistry

authors： ElAntak L,Dolla A,Durand MC,Bianco P,Guerlesquin F

更新日期：2005-11-15 00:00:00

abstract：:D(--)-beta-Hydroxybutyrate dehydrogenase of beef heart mitochondria is inhibited by modifiers of disulfides, thiols, and vicinal dithiols [Phelps, D. C., & Hatefi, Y. (1981) Biochemistry 20 (preceding paper in this issue)]. The vicinal dithiol can be reversibly oxidized by diamide, resulting in activity inhibition, an...

journal_title：Biochemistry

authors： Phelps DC,Hatefi Y

更新日期：1981-02-03 00:00:00

abstract：:The hypothesis that the stable ternary complex formed between myosin subfragment-1, MgADP and beryllium fluoride (BeF3-), denoted S-1 not equal to .ADP.BeF3-, is an analog of the intermediate state S-1**.ADP.P(i) has been tested in this work by examining the interactions of S-1 not equal to .ADP.BeF3- with actin. Equi...

journal_title：Biochemistry

authors： Phan BC,Faller LD,Reisler E

更新日期：1993-08-03 00:00:00

abstract：:Imidazole glycerol phosphate synthase (ImGPS) from Thermotoga maritima is a model enzyme for studying allostery. The ImGPS complex consists of the cyclase subunit HisF and the glutaminase subunit HisH whose activity is stimulated by substrate binding to HisF in a V-type manner. To investigate the significance of a put...

journal_title：Biochemistry

authors： Kneuttinger AC,Rajendran C,Simeth NA,Bruckmann A,König B,Sterner R

更新日期：2020-07-28 00:00:00

abstract：:We report the use of electrospray ionization (ESI) mass spectrometry (MS) in conjunction with online rapid mixing to monitor the kinetics of acid-induced ferrihemoglobin denaturation. Under equilibrium conditions, the hemoglobin mass spectrum is dominated by the intact heterotetramer. Dimeric and monomeric species are...

journal_title：Biochemistry

authors： Simmons DA,Wilson DJ,Lajoie GA,Doherty-Kirby A,Konermann L

更新日期：2004-11-23 00:00:00

abstract：:Vascular adhesion molecules regulate the migration of leukocytes from the blood into tissue during inflammation. Binding of leukocytes to vascular cell adhesion molecule-1 (VCAM-1) activates signals in endothelial cells, including Rac1 and calcium fluxes. These VCAM-1 signals are required for leukocyte transendothelia...

journal_title：Biochemistry

authors： Marchese ME,Berdnikovs S,Cook-Mills JM

更新日期：2012-10-16 00:00:00

abstract：:The ligation state of hemoglobin during its cross-linking by glycolaldehyde influences the ultimate oxygen affinity of the cross-linked protein. Thus, if the cross-linking is performed with carbonmonoxy-hemoglobin, the oxygen affinity increases slightly to a P50 of 7 mmHg from a P50 of 9 mmHg for unmodified hemoglobin...

journal_title：Biochemistry

authors： Manning LR,Manning JM

更新日期：1988-08-23 00:00:00

abstract：:Exoenzyme S (ExoS) is a mono-ADP-ribosyltransferase secreted by the opportunistic pathogen Pseudomonas aeruginosa. ExoS requires a eukaryotic factor, the 14-3-3 protein, for enzymatic activity. Here, two aspects of the activation of the ADP-ribosyltransferase activity of ExoS by 14-3-3 proteins are examined. Initial s...

journal_title：Biochemistry

authors： Zhang L,Wang H,Masters SC,Wang B,Barbieri JT,Fu H

更新日期：1999-09-14 00:00:00

abstract：:Single lamellar phosphatidyl[methyl-2H]choline vesicles were incubated with an excess of unlabeled phosphatidylcholine vesicles or phosphatidylcholine-cholesterol vesicles containing 8 mol % glucuronosyldiglyceride. Incubation of the two vesicle populations was performed in the presence or absence of a purified phosph...

journal_title：Biochemistry

authors： Shaw JM,Thompson TE

更新日期：1982-03-02 00:00:00

abstract：:A detailed kinetic analysis of the catalytic trimer of aspartate transcarbamoylase containing the active site substitution H134A was performed to investigate the role of His 134 in the catalytic mechanism. Replacement of histidine by alanine resulted in decreases in the affinities for the two substrates, carbamoyl pho...

journal_title：Biochemistry

authors： Waldrop GL,Turnbull JL,Parmentier LE,O'Leary MH,Cleland WW,Schachman HK

更新日期：1992-07-21 00:00:00

abstract：:Reticulocyte lysate contains a chaperone system that assembles glucocorticoid receptor (GR).hsp90 heterocomplexes. Using purified proteins, we have prepared a five-protein heterocomplex assembly system consisting of two proteins essential for heterocomplex assembly-hsp90 and hsp70-and three proteins that act as co-cha...

journal_title：Biochemistry

authors： Kanelakis KC,Murphy PJ,Galigniana MD,Morishima Y,Takayama S,Reed JC,Toft DO,Pratt WB

更新日期：2000-11-21 00:00:00

abstract：:In this study, we have mapped the intracellular alkylation sites of adozelesin and bizelesin, two potent analogs of CC-1065, in individual genes at the single-nucleotide level. Human colon carcinoma cells were treated with adozelesin and bizelesin, and the position of adducts were mapped within the PGK-1 and p53 genes...

journal_title：Biochemistry

authors： Lee CS,Pfeifer GP,Gibson NW

更新日期：1994-05-17 00:00:00

abstract：:Although the terms "excessive reactive oxygen species (ROS)" and "oxidative stress" are widely used, the implications of oxidative stress are often misunderstood. ROS are not a single species but a variety of compounds, each with unique biochemical properties and abilities to react with biomolecules. ROS cause activat...

journal_title：Biochemistry

authors： AbdulSalam SF,Thowfeik FS,Merino EJ

更新日期：2016-09-27 00:00:00

abstract：:NADH oxidase activity has been detected in luminal membrane derived from the proximal tubules of rat kidney (brush border membranes). The cytochromes associated with these membranes [García, M.L., Benavides, J., Valdivieso, F., Mayor, F., & Giménez-Gallego, G. (1978) Biochem. Biophys. Res. Commun. 82, 738-744] were re...

journal_title：Biochemistry

authors： Giménez-Gallego G,Benavides J,García ML,Valdivieso F

更新日期：1980-10-14 00:00:00

abstract：:The synthesis of sphingomyelin was studied in baby hamster kidney cells and in subcellular fractions derived from rat liver. During pulse-chase experiments with [3H]choline in tissue culture cells, the specific radioactivity of sphingomyelin continued to increase after the specific activities of phosphocholine and cyt...

journal_title：Biochemistry

authors： Voelker DR,Kennedy EP

更新日期：1982-05-25 00:00:00

abstract：:Chemoenzymatic glycan remodeling by endoglycosidase-catalyzed deglycosylation and reglycosylation is emerging as an attractive approach for producing homogeneous glycoforms of antibodies, and the success of this approach depends on the discovery of efficient endoglycosidases and their glycosynthase mutants. We report ...

journal_title：Biochemistry

authors： Tong X,Li T,Li C,Wang LX

更新日期：2018-09-04 00:00:00

abstract：:Cystathionine β-synthase (CBS) catalyzes the first step in the transsulfuration pathway in mammals, i.e., the condensation of serine and homocysteine to produce cystathionine and water. Recently, we have reported a steady-state kinetic analysis of the three hydrogen sulfide (H(2)S)-generating reactions that are cataly...

journal_title：Biochemistry

authors： Singh S,Ballou DP,Banerjee R

更新日期：2011-01-25 00:00:00