Abstract：
:This report presents evidence for interdomain steps of the ligand-coupled signal transduction mechanism of the oxygen receptor from Rhizobium meliloti, RmFixL. Photolysis of the CO adducts of heme domain (RmFixLN) and heme kinase (RmFixL*) proteins allowed tracking of second-order heme CO recombination reactions by transient absorbance. Whereas CO rebinding to RmFixLN is characterized by a single kinetic phase, rebinding to RmFixL* is characterized by two kinetic phases. Evidence indicates that CO rebinds to two interconvertible deoxyRmFixL* conformers that are produced sequentially after photolysis. Since the second conformer is only observed when the kinase domain is present, its production is concluded to be an interdomain signal transmission event that is coupled to heme ligand release. Because receptor clustering is a recurring theme in signal transduction mechanisms, the dependence of molecular weight upon heme ligation was investigated at equilibrium. Gel permeation chromatography and native gel electrophoresis showed that the molecular weight distribution for both RmFixLN and RmFixL* depends on heme ligation. At equilibrium, oxyRmFixLN and oxyRmFixL* exist as monomers and dimers, respectively. Their deoxy analogues, metRmFixLN and metRmFixL*, exist as dimers and as a mixture of tetramers and 9-mers, respectively. Assembly of these oligomers is reversible. The physiological relevance of these ligand-coupled assemblies and the kinetic factors controlling CO recombination are discussed.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Rodgers KR,Tang L,Lukat-Rodgers GS,Wengenack NLdoi
10.1021/bi011237qsubject
Has Abstractpub_date
2001-10-30 00:00:00pages
12932-42issue
43eissn
0006-2960issn
1520-4995pii
bi011237qjournal_volume
40pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The photolyase/cryptochrome family is a large family of flavoproteins that encompasses DNA repair proteins, photolyases, and cryptochromes that regulate blue-light-dependent growth and development in plants, and light-dependent and light-independent circadian clock setting in animals. Phylogenetic analysis has reveale...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi801085a
更新日期：2008-09-30 00:00:00
abstract：:Cells can be transiently permeabilized by application of high electric pulses of short duration. A direct consequence of this treatment is to induce a fusogenic state in the pulsed membrane. The molecular events underlying these phenomena remain to be explained. During our work, we investigated the effects of pulsing ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00471a009
更新日期：1990-05-15 00:00:00
abstract：:Oligonucleotide-directed mutagenesis in vivo was used to create destabilizing site specific changes at position 6 in iso-1-cytochrome c in Saccharomyces cerevisiae. These changes were made in combination with the stabilizing N52I substitution. The resulting proteins showed that a variety of forces and interactions are...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00021a023
更新日期：1995-05-30 00:00:00
abstract：:Homoisocitrate dehydrogenase (HIcDH, 3-carboxy-2-hydroxyadipate dehydrogenase) catalyzes the fourth reaction of the alpha-aminoadipate pathway for lysine biosynthesis, the conversion of homoisocitrate to alpha-ketoadipate using NAD as an oxidizing agent. A chemical mechanism for HIcDH is proposed on the basis of the p...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi702361j
更新日期：2008-04-01 00:00:00
abstract：:The solution structure of the 63-residue heregulin-alpha (HRG-alpha) epidermal growth factor (EGF)-like domain, corresponding to residues 177-239 of HRG-alpha, has been determined to high resolution using data from two-dimensional and three-dimensional homo- and heteronuclear NMR spectroscopy. The structure is based o...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi952626l
更新日期：1996-03-19 00:00:00
abstract：:With respect to the functional importance attributed to the N-terminal part of the Na(+)/proline transporter of Escherichia coli (PutP), we report here on the structural arrangement and functional dynamics of transmembrane domains (TMs) II and III and the adjoining loop regions. Information on membrane topography was ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi992442x
更新日期：2000-04-25 00:00:00
abstract：:Inosine has the ability to act as a "wild-card" binding nonspecifically to both A.T and G.C base pairs. This has obvious implications for the design of oligonucleotide site-directed probes. In this paper we present a series of oligonucleotides with a 5'pur9-pyr9-pyr9 motif which are designed to fold up sequentially in...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi960193w
更新日期：1996-10-15 00:00:00
abstract：:Organisms and cellular systems which have adapted to stresses such as high temperature, desiccation, and urea-concentrating environments have responded by concentrating particular organic solutes known as osmolytes. These osmolytes are believed to confer protection to enzyme and other macromolecular systems against su...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00138a006
更新日期：1992-06-16 00:00:00
abstract：:The epidermal growth factor receptor (EGFR) kinase is activated by a variety of mutations in human cancers. R776H is one such recurrent mutation (R752H in another numbering system) in the αC-β4 loop of the tyrosine kinase domain that activates EGFR in the absence of the activating EGF ligand. However, the mechanistic ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00444
更新日期：2015-07-14 00:00:00
abstract：:Prolyl oligopeptidase B from Galerina marginata (GmPOPB) has recently been discovered as a peptidase capable of breaking and forming peptide bonds to yield a cyclic peptide. Despite the relevance of prolyl oligopeptidases in human biology and disease, a kinetic analysis pinpointing rate-limiting steps for a member of ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.7b00012
更新日期：2017-04-18 00:00:00
abstract：:An affinity labeling technique has been developed to identify the folate-methotrexate transporter of Leishmania donovani promastigotes using "activated" derivatives of the ligands. These "activated" derivatives were synthesized by incubating folate and methotrexate with a 10-fold excess of 1-ethyl-3-[3-(dimethylamino)...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00443a023
更新日期：1989-08-22 00:00:00
abstract：:The beta-glycosidase from the hyperthermophilic Archaeon Sulfolobus solfataricus hydrolyzes beta-glycosides following a retaining mechanism based upon the action of two amino acids: Glu387, which acts as the nucleophile of the reaction, and Glu206, which acts as the general acid/base catalyst. The activities of inacti...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi981855f
更新日期：1998-12-08 00:00:00
abstract：:The pigment composition of the isolated photosystem II reaction center complex in its most stable and pure form currently is a matter of considerable debate. In this contribution, we present a new method based on a combination of gel filtration chromatography and diode array detection to analyze the composition of pho...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi960991m
更新日期：1996-10-01 00:00:00
abstract：:Staphylococcal fibronectin-binding protein (FnbA) is a surface-associated receptor responsible for the reversible binding of bacteria to human fibronectin and fibrin(ogen). Recently we have shown that FnbA serves as a substrate for coagulation factor XIIIa and undergoes covalent cross-linking to its ligands, resulting...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi049278k
更新日期：2004-09-21 00:00:00
abstract：:Bovine hoof keratin was shown to be a substrate for cAMP-dependent protein kinase using [gamma-32P]ATP. Natural-abundance cross-polarization (CP) MAS 13C NMR was used to examine the effect of phosphorylation on keratin structure. When short contact times were used, phosphorylation was shown to increase the number of r...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00458a023
更新日期：1990-02-13 00:00:00
abstract：:Human genomic clones that span the entire protein S expressed gene (PS alpha) and the 3' two-thirds of the protein S pseudogene (PS beta) have been isolated and characterized. The PS alpha gene is greater than 80 kilobases in length and contains 14 introns and 15 exons, as well as 6 repetitive "Alu" sequences. Exons I...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00486a010
更新日期：1990-08-28 00:00:00
abstract：:Patas monkeys were studied for 2 years on three dietary regimes: (1) commercial chow (control diet); (2) semipurified diet plus lard (fat-fed); and (3) semipurified diet plus lard and cholesterol (cholesterol-fed). The control and fat-fed animals had similar lipoproteins which were equivalent to the human very low den...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00659a007
更新日期：1976-07-13 00:00:00
abstract：:Magainin and PGLa are 23- and 21-residue peptides isolated from the skin of the African clawed frog Xenopus laevis. They protect the frog from infection and exhibit a broad-spectrum antimicrobial activity in vitro. The mechanism of this activity involves the interaction of magainin with microbial membranes. We have me...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00470a031
更新日期：1990-05-08 00:00:00
abstract：:The physical origin of the extremely high thermal stability of tetraether liposomes composed of the polar lipid fraction E (PLFE) from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius has been investigated. The leakage rate of trapped 5, 6-carboxyfluorescein (5(6)CF) and the proton permeability in PLFE ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi972163e
更新日期：1998-01-06 00:00:00
abstract：:A study of the regular secondary structure elements of recombinant human interleukin-1 beta has been carried out using NMR spectroscopy. Using a randomly 15N labeled sample, a number of heteronuclear three- and two-dimensional NMR experiments have been performed, which have enabled a complete analysis of short-, mediu...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00471a023
更新日期：1990-05-15 00:00:00
abstract：:The first structure of a glycerol kinase from a Gram-positive organism, Enterococcus casseliflavus, has been determined to 2.8 A resolution in the presence of glycerol and to 2.5 A resolution in the absence of substrate. The substrate-induced closure of 7 degrees is significantly smaller than that reported for hexokin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi034258o
更新日期：2004-01-20 00:00:00
abstract：:The members of the glutamine amidotransferase (GATase) family catalyze the incorporation of ammonia within numerous metabolic pathways and can be categorized in two classes. Here, we concentrated on class I GATases, which are heteromeric enzyme complexes consisting of synthase subunits and glutaminase subunits with a ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.9b00286
更新日期：2019-06-04 00:00:00
abstract：:The folding behavior of bovine growth hormone (bGH) is examined by chemical and pH denaturation using several spectroscopic probes of protein secondary and tertiary structure. Partially denaturing concentrations of urea eliminate the native-state quenching of intrinsic tryptophan fluorescence, from the single protein ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00457a022
更新日期：1990-02-06 00:00:00
abstract：:We have investigated the action of the human DNA polymerase ε (hpol ε) and η (hpol η) catalytic cores on G-quadruplex (G4) DNA substrates derived from the promoter of the c-MYC proto-oncogene. The translesion enzyme hpol η exhibits a 6.2-fold preference for binding to G4 DNA over non-G4 DNA, while hpol ε binds both G4...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00060
更新日期：2015-05-26 00:00:00
abstract：:Self-assembly properties of HIV-1 integrase were investigated by time-resolved fluorescence anisotropy using tryptophanyl residues as a probe. From simulation analyses, we show that suitable photon counting leads to an accurate determination of long rotational correlation times in the range of 20-80 ns, permitting the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi000397j
更新日期：2000-08-08 00:00:00
abstract：:The site-directed mutagenesis of a number of proposed active site residues of 5-enolpyruvyl shikimate-3-phosphate (EPSP) synthase is reported. Several of these mutations resulted in complete loss of enzyme activity indicating that these residues are probably involved with catalysis, notably K22R, K411R, D384A, R27A, R...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9815142
更新日期：1999-01-05 00:00:00
abstract：:Isopentenyl phosphate kinase (IPK) catalyzes the phosphorylation of isopentenyl phosphate to form the isoprenoid precursor isopentenyl diphosphate in the archaeal mevalonate pathway. This enzyme is highly homologous to fosfomycin kinase (FomA), an antibiotic resistance enzyme found in a few strains of Streptomyces and...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi201662k
更新日期：2012-01-31 00:00:00
abstract：:Ketopantoate reductase (EC 1.1.1.169) catalyzes the NADPH-dependent reduction of alpha-ketopantoate to form D-(-)-pantoate in the pantothenate/coenzyme A biosynthetic pathway. The enzyme encoded by the panE gene from E. coli K12 was overexpressed and purified to homogeneity. The native enzyme exists in solution as a m...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi992676g
更新日期：2000-04-04 00:00:00
abstract：:In the commonly used nucleation-dependent model of protein aggregation, aggregation proceeds only after a lag phase in which the concentration of energetically unfavorable nuclei reaches a critical value. The formation of oligomeric species prior to aggregation can be difficult to detect by current spectroscopic techn...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi400027y
更新日期：2013-03-19 00:00:00
abstract：:The reaction of cytochrome c oxidase with dioxygen has been studied by means of time-resolved measurements of electrical membrane potential (DeltaPsi). Microsecond time resolution was achieved by starting with the CO-inhibited enzyme, which was photolyzed after addition of oxygen. The time course of the reaction could...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi982275l
更新日期：1999-03-02 00:00:00
