Restoration of the activity of active-site mutants of the hyperthermophilic beta-glycosidase from Sulfolobus solfataricus: dependence of the mechanism on the action of external nucleophiles.

abstract：:Human apolipoprotein stimulation of sphingomyelin (SM) hydrolysis by sphingomyelinase from human skin fibroblasts has been studied. Apolipoproteins A-I, A-II, B, C-I, and E do not enhance sphingomyelin hydrolysis above control levels. In contrast, apoC-II stimulates sphingomyelin hydrolysis by approximately 2.5-fold. ...

journal_title：Biochemistry

authors： Ahmad TY,Beaudet AL,Sparrow JT,Morrisett JD

更新日期：1986-07-29 00:00:00

abstract：:Phage display technique is a powerful tool with which to identify novel binding sequences for antibody and receptor targets. Few studies, however, have used this technology to select affinity peptides for ligand molecules. Here, we screened a peptide phage library for binding to toxic shock syndrome toxin 1 (TSST-1) t...

journal_title：Biochemistry

authors： Sato A,Ida N,Fukuyama M,Miwa K,Kazami J,Nakamura H

更新日期：1996-08-13 00:00:00

abstract：:A series of truncated forms of His(6)-tagged gp91phox were expressed, solubilized, and purified in the presence of 30 microM FAD. The truncated gp91phox with the longest sequence in the C-terminal region (221-570) (gp91C) showed the highest activity (turnover rate, 0.92) for NADPH diaphorase in the presence of either ...

journal_title：Biochemistry

authors： Nisimoto Y,Ogawa H,Miyano K,Tamura M

更新日期：2004-07-27 00:00:00

abstract：:One way to improve the economic feasibility of biomass conversion is to enhance the catalytic efficiency of cellulases through protein engineering. This requires that high-resolution structures of cellulases be available. Here we present the structure of E2cd, the catalytic domain of the thermophilic endocellulase E2 ...

journal_title：Biochemistry

authors： Spezio M,Wilson DB,Karplus PA

更新日期：1993-09-28 00:00:00

abstract：:The radiation inactivation method was used to study the size of acetylcholine receptors in the intact membrane-bound state. This technique was reinvestigated, and modifications were made which remove substantial difficulties affecting previous applications of it to such proteins. The molecular size was deduced here by...

journal_title：Biochemistry

authors： Lo MM,Barnard EA,Dolly JO

更新日期：1982-04-27 00:00:00

abstract：:Human S100A7 (psoriasin) is a metal-chelating protein expressed by epithelial cells. It is a 22-kDa homodimer with two EF-hand domains per subunit and two transition-metal-binding His3Asp sites at the dimer interface. Each subunit contains two cysteine residues that can exist as free thiols (S100A7red) or as an intram...

journal_title：Biochemistry

authors： Cunden LS,Brophy MB,Rodriguez GE,Flaxman HA,Nolan EM

更新日期：2017-10-31 00:00:00

abstract：:A considerable body of data supports the model that the infectious agent (called a prion) which causes the transmissible spongiform encephalopathies is a replicating polypeptide devoid of nucleic acid. Prions are believed to propagate by changing the conformation of the normal cellular prion protein (PrPc) into an inf...

journal_title：Biochemistry

authors： Cappai R,Stewart L,Jobling MF,Thyer JM,White AR,Beyreuther K,Collins SJ,Masters CL,Barrow CJ

更新日期：1999-03-16 00:00:00

abstract：:Fibronectin (FN) is an extracellular matrix (ECM) protein found soluble in corporal fluids or as an insoluble fibrillar component incorporated in the ECM. This phenomenon implicates structural changes that expose FN binding sites and activate the protein to promote intermolecular interactions with other FN. We have in...

journal_title：Biochemistry

authors： Patel S,Chaffotte AF,Goubard F,Pauthe E

更新日期：2004-02-17 00:00:00

abstract：:It has been shown (Poole et al., 1991) that deletion of residues 44-49 from the sequence of staphylococcal nuclease (E43 SNase) results in an enzyme (E43 delta SNase) that is significantly more active than D43 SNase, an enzyme that differs from the wild-type enzyme by deletion of a single methylene group. In addition,...

journal_title：Biochemistry

authors： Baldisseri DM,Torchia DA,Poole LB,Gerlt JA

更新日期：1991-04-16 00:00:00

abstract：:The neutron structure of subtilisin BPN' has been refined and analyzed at 2.0-A resolution. The structure studied was a mutant variant of subtilisin, Met222----Gln, and was used because large, uninhibited crystals could be grown, which was not the case for the native molecule. Comparison of the structure with that of ...

journal_title：Biochemistry

authors： Kossiakoff AA,Ultsch M,White S,Eigenbrot C

更新日期：1991-02-05 00:00:00

abstract：:Coenzyme A (CoA)-transferases catalyze transthioesterification reactions involving acyl-CoA substrates, using an active-site carboxylate to form covalent acyl anhydride and CoA thioester adducts. Mechanistic studies of class I CoA-transferases suggested that acyl-CoA binding energy is used to accelerate rate-limiting ...

journal_title：Biochemistry

authors： Mullins EA,Kappock TJ

更新日期：2012-10-23 00:00:00

abstract：:Microfibrils having a diameter of 10-12 nm, found either in association with elastin or independently, are an important component of the extracellular matrix of many tissues. Because isolation of native proteins composing the microfibrils has proven difficult, information on structure/function relationships is limited...

journal_title：Biochemistry

authors： Bashir MM,Abrams WR,Rosenbloom J,Kucich U,Bacarra M,Han MD,Brown-Augsberger P,Mecham R,Rosenbloom J

更新日期：1994-01-18 00:00:00

abstract：:Histidine decarboxylase (HDC) from Lactobacillus 30a forms a trimer around a central cavity or well. Three active sites are formed around the well at the interface of each of two adjacent molecules. HDC exhibits cooperative kinetics at pH 7.6 and can be described in terms of a two-state, T and R, model. At pH 4.8, pro...

journal_title：Biochemistry

authors： Pishko EJ,Potter KA,Robertus JD

更新日期：1995-05-09 00:00:00

abstract：:The N-terminal extracellular parts of human G-protein coupled receptor class B, for example, receptors for secretin, glucagon, or parathyroid hormone, are involved in ligand binding. To obtain structural and functional information on the N-terminal receptor fragment of human parathyroid hormone receptor 1 (PTHR1), the...

journal_title：Biochemistry

authors： Grauschopf U,Lilie H,Honold K,Wozny M,Reusch D,Esswein A,Schäfer W,Rücknagel KP,Rudolph R

更新日期：2000-08-01 00:00:00

abstract：:The cro repressor protein from bacteriophage lambda has been studied in solution by two-dimensional nuclear magnetic resonance spectroscopy (2D NMR). Following the approach of Wüthrich and co-workers [Wüthrich, K., Wider, G., Wagner, G., & Braun, W. (1982) J. Mol. Biol. 155, 311-319], individual spin systems were iden...

journal_title：Biochemistry

authors： Weber PL,Wemmer DE,Reid BR

更新日期：1985-08-13 00:00:00

abstract：:The lipid fatty acid composition of the cytoplasmic membranes of Escherichia coli can be varied by growing an unsaturated fatty acid auxotroph in the presence of different fatty acid supplements. Electron spin resonance (ESR) studies of spin-label partitioning into the cytoplasmic membranes of different lipid fatty ac...

journal_title：Biochemistry

authors： Linden CD,Blasie JK,Fox CF

更新日期：1977-04-19 00:00:00

abstract：:Several bifunctional alkylating agents of the aziridinylbenzoquinone class have been evaluated as potential antitumor agents. 3,6-Bis[(2-hydroxyethyl)amino]-2,5- diaziridinyl-1,4-benzoquinone (BZQ), 2,5-diaziridinyl-1,4-benzoquinone (DZQ), 3,6-bis(carboxyamino)-2,5-diaziridinyl- 1,4-benzoquinone (AZQ), and six analogu...

journal_title：Biochemistry

authors： Hartley JA,Berardini M,Ponti M,Gibson NW,Thompson AS,Thurston DE,Hoey BM,Butler J

更新日期：1991-12-17 00:00:00

abstract：:4-Oxalocrotonate tautomerase (4-OT) and YwhB, a 4-OT homologue found in Bacillus subtilis, exhibit a low level hydratase activity that converts trans-3-haloacrylates to acetaldehyde, presumably through a malonate semialdehyde intermediate. The mechanism for the initial transformation of the 3-haloacrylate to malonate ...

journal_title：Biochemistry

authors： Wang SC,Johnson WH Jr,Czerwinski RM,Whitman CP

更新日期：2004-01-27 00:00:00

abstract：:Resonance energy transfer measurements provide a way to estimate distances between chromophores attached to different sites of macromolecules. There are two unknowns involved in resonance energy transfer measurements, the distance between two chromophores and their relative orientation. When static orientational disor...

journal_title：Biochemistry

authors： Wu P,Brand L

更新日期：1992-09-01 00:00:00

abstract：:Cibacron Blue, a widely used ligand for affinity chromatography, is a potent inhibitor of NAD(P)H:(quinone-acceptor) oxidoreductase (EC 1.6.99.2) (quinone reductase). This property has been exploited to purify quinone reductase, to identify its nucleotide-binding site, and to obtain diffraction-grade crystals of this ...

journal_title：Biochemistry

authors： Prestera T,Prochaska HJ,Talalay P

更新日期：1992-01-28 00:00:00

abstract：:Depletion of functional Ca2+ from photosystem (PS) II membranes impairs O2 evolution. Redox properties of the Mn cluster as probed by thermoluminescence were modified differently in Ca(2+)-depleted PSII depending on the procedure for Ca2+ extraction. Ca2+ depletion by low-pH treatment gave rise to an abnormally modifi...

journal_title：Biochemistry

authors： Ono T,Izawa S,Inoue Y

更新日期：1992-08-25 00:00:00

abstract：:The substrate specificity of the beta-glucosidase (CelB) from the hyperthermophilic archaeon Pyrococcus furiosus, a family 1 glycosyl hydrolase, has been studied at a molecular level. Following crystallization and X-ray diffraction of this enzyme, a 3.3 A resolution structural model has been obtained by molecular repl...

journal_title：Biochemistry

authors： Kaper T,Lebbink JH,Pouwels J,Kopp J,Schulz GE,van der Oost J,de Vos WM

更新日期：2000-05-02 00:00:00

abstract：:In an effort to characterize the bimolecular interface between parathyroid hormone (PTH) and its human receptor PTH1-Rc (hPTH1-Rc), we previously identified two contact sites in the receptor: one for position 1 and another for position 13 (located at the ends of the principal activation domain) in PTH(1-34). The prese...

journal_title：Biochemistry

authors： Greenberg Z,Bisello A,Mierke DF,Rosenblatt M,Chorev M

更新日期：2000-07-18 00:00:00

abstract：:The P-glycoprotein multidrug transporter functions as an ATP-driven efflux pump for a large number of structurally unrelated hydrophobic compounds. Substrates are believed to gain access to the transporter after partitioning into the membrane, rather than from the extracellular aqueous phase. The binding of drug subst...

journal_title：Biochemistry

authors： Romsicki Y,Sharom FJ

更新日期：1999-05-25 00:00:00

abstract：:The σ1 receptor is an integral membrane protein that shares no homology with other receptor systems, has no unequivocally identified natural ligands, but appears to play critical roles in a wide variety of cell functions. While the number of reports of the possible functions of the σ1 receptor is increasing, almost no...

journal_title：Biochemistry

authors： Brune S,Schepmann D,Klempnauer KH,Marson D,Dal Col V,Laurini E,Fermeglia M,Wünsch B,Pricl S

更新日期：2014-05-13 00:00:00

abstract：:A gene encoding the high-potential iron-sulfur protein (HiPIP) was cloned from the purple photosynthetic bacterium Rubrivivax gelatinosus. An insertional disruption of this gene by a kanamycin resistance cartridge resulted in a significant decrease in the growth rate under photosynthetic growth conditions. Flash-induc...

journal_title：Biochemistry

authors： Nagashima KV,Matsuura K,Shimada K,Verméglio A

更新日期：2002-11-26 00:00:00

abstract：:Understanding the molecular mechanisms of enzyme catalysis and allosteric regulation has been a primary goal of biochemistry for many years. The dynamics of these processes, approached through a variety of kinetic methods, are discussed. The results obtained for many different enzymes suggest that multiple intermediat...

journal_title：Biochemistry

authors： Hammes GG

更新日期：2002-07-02 00:00:00

abstract：:The single-stranded packaged genome (ssDNA) of bacteriophage phi X174 is shown by Raman spectroscopy to lack both the ordered phosphodiester backbone and base stacking, which are demonstrated for unpackaged, protein-free ssDNA. In solutions of moderate ionic strength, unpackaged ssDNA contains 36 +/- 7% of deoxyribosy...

journal_title：Biochemistry

authors： Benevides JM,Stow PL,Ilag LL,Incardona NL,Thomas GJ Jr

更新日期：1991-05-21 00:00:00

abstract：:The (1)H NMR resonances of the heme substituents of the low-spin Fe(III) form of nitrophorin 2, as its complexes with N-methylimidazole (NP2-NMeIm) and imidazole (NP2-ImH), have been assigned by a combination of (1)H homonuclear two-dimensional NMR techniques and (1)H-(13)C HMQC. Complete assignment of the proton and ...

journal_title：Biochemistry

authors： Shokhireva TKh,Shokhirev NV,Walker FA

更新日期：2003-01-28 00:00:00

abstract：:Applications of sensitive new technologies, in particular, two-dimensional NMR spectroscopy, have allowed detection of folded structures in short peptide fragments of proteins in aqueous solution under conditions where native proteins fold. These structures are in rapid dynamic exchange with unfolded states. These obs...

journal_title：Biochemistry

authors： Wright PE,Dyson HJ,Lerner RA

更新日期：1988-09-20 00:00:00