In vitro kinetic analysis of substrate specificity in enterobactin biosynthetic lower pathway enzymes provides insight into the biochemical function of the hot dog-fold thioesterase EntH.

abstract：:Pili are surface-linked virulence factors that play key roles in infection establishment in a variety of pathogenic species. In Gram-positive pathogens, pilus formation requires the action of sortases, dedicated transpeptidases that covalently associate pilus building blocks. In Streptococcus pneumoniae, a major human...

journal_title：Biochemistry

authors： Manzano C,Izoré T,Job V,Di Guilmi AM,Dessen A

更新日期：2009-11-10 00:00:00

abstract：:The clathrin assembly protein complex AP-2 is a multimeric subunit complex consisting of two 100-115-kDa subunits known as alpha and beta and 50- and 16-kDa subunits. The subunits have been dissociated and separated by ion-exchange chromatography in 7.5 M urea. Fractions highly enriched in either the alpha or beta sub...

journal_title：Biochemistry

authors： Prasad K,Keen JH

更新日期：1991-06-04 00:00:00

abstract：:The targeting of the insulin-responsive glucose transporter, GLUT-4, to an intracellular compartment in adipocytes and muscle is one of the key features responsible for the unique insulin sensitivity of this transporter. Through expression of epitope-tagged GLUT-4 mutants in 3T3-L1 adipocytes, two motifs have been ide...

journal_title：Biochemistry

authors： Melvin DR,Marsh BJ,Walmsley AR,James DE,Gould GW

更新日期：1999-02-02 00:00:00

abstract：:Neuronal PAS protein 2 (NPAS2), a heme-binding transcriptional regulatory factor, is involved in circadian rhythms. Period homologue (Per) is another important transcriptional regulatory factor that binds to cryptochrome (Cry). The resultant Per/Cry heterodimer interacts with the NPAS2/BMAL1 heterodimer to inhibit the...

journal_title：Biochemistry

authors： Kitanishi K,Igarashi J,Hayasaka K,Hikage N,Saiful I,Yamauchi S,Uchida T,Ishimori K,Shimizu T

更新日期：2008-06-10 00:00:00

abstract：:Paranemic crossover DNA (PX-DNA) is a four-stranded multicrossover structure that has been implicated in recombination-independent recognition of homology. Although existing evidence has suggested that PX is the DNA motif in homologous pairing (HP), this conclusion remains ambiguous. Further investigation is needed bu...

journal_title：Biochemistry

authors： Kizer M,Huntress ID,Walcott BD,Fraser K,Bystroff C,Wang X

更新日期：2019-03-12 00:00:00

abstract：:The function of the GM2 activator protein is to act as a substrate-specific cofactor in the hydrolysis of GM2 ganglioside by beta-hexosaminidase A. Mutations in the gene encoding it result in the AB variant form of GM2 gangliosidosis. One such mutation, Cys138 Arg, results in the mutant protein being retained and degr...

journal_title：Biochemistry

authors： Xie B,Rigat B,Smiljanic-Georgijev N,Deng H,Mahuran D

更新日期：1998-01-20 00:00:00

abstract：:The structural behavior of the nucleosome core particles in the range of solvent Na+ concentration from 10.45 to 0.45 mM has been studied by small-angle neutron and synchroton radiation X-ray scattering, sedimentation, atomic absorption spectroscopy, density measurements, and circular dichroism. With decreasing salt c...

journal_title：Biochemistry

authors： Hirai M,Niimura N,Zama M,Mita K,Ichimura S,Tokunaga F,Ishikawa Y

更新日期：1988-10-04 00:00:00

abstract：:Galactosyl- and glucosylceramide, globoside, and dihydrolactosylceramide, bearing [2,2-2H2]stearic acid, have been studied at a concentration of 10 mol% in bilayers of dimyristoylphosphatidylcholine by 2H NMR. The quadrupolar splitting delta vQ of the C2 deuterons were measured at several temperatures in the range of ...

journal_title：Biochemistry

authors： Fenske DB,Hamilton K,Jarrell HC,Florio E,Barber KR,Grant CW

更新日期：1991-05-07 00:00:00

abstract：:In an irreversible enzyme-catalyzed reaction, strong binding of the products would lead to substantial product inhibition. The X-ray crystal structure of the product complex of thymidylate synthase (1.83-A resolution, R factor = 0.183 for all data between 7.0 and 1.83 A) identifies a bound water molecule that serves t...

journal_title：Biochemistry

authors： Fauman EB,Rutenber EE,Maley GF,Maley F,Stroud RM

更新日期：1994-02-15 00:00:00

abstract：:Cibacron Blue, a widely used ligand for affinity chromatography, is a potent inhibitor of NAD(P)H:(quinone-acceptor) oxidoreductase (EC 1.6.99.2) (quinone reductase). This property has been exploited to purify quinone reductase, to identify its nucleotide-binding site, and to obtain diffraction-grade crystals of this ...

journal_title：Biochemistry

authors： Prestera T,Prochaska HJ,Talalay P

更新日期：1992-01-28 00:00:00

abstract：:We have reexamined the binding properties of the antitumor drug daunomycin using double-helical oligonucleotides 16 base pairs long that were designed to contain preferred binding sites for the drug. The preferred sites are contained in a six base pair core which is flanked on the 5' and 3' ends by tracts of adenines....

journal_title：Biochemistry

authors： Roche CJ,Thomson JA,Crothers DM

更新日期：1994-02-01 00:00:00

abstract：:Endothelium-derived epoxyeicosatrienoic acids (EETs) relax vascular smooth muscle by activating potassium channels and causing membrane hyperpolarization. Recent evidence suggests that EETs act via a membrane binding site or receptor. To further characterize this binding site or receptor, we synthesized 20-iodo-14,15-...

journal_title：Biochemistry

authors： Chen Y,Falck JR,Manthati VL,Jat JL,Campbell WB

更新日期：2011-05-10 00:00:00

abstract：:Faithful translation of the genetic code is mainly based on the specificity of tRNA aminoacylation catalyzed by aminoacyl-tRNA synthetases. These enzymes are comprised of a catalytic core and several appended domains. Bacterial glutamyl-tRNA synthetases (GluRS) contain five structural domains, the two distal ones inte...

journal_title：Biochemistry

authors： Dubois DY,Blais SP,Huot JL,Lapointe J

更新日期：2009-06-30 00:00:00

abstract：:C-type natriuretic peptide (CNP) binds the guanylyl cyclase-linked natriuretic peptide receptor B (NPR-B) and stimulates marked elevations of the intracellular signaling molecule, cGMP. Here, the essential role of phosphorylation in the hormonal activation and deactivation of this receptor is described. Exposure of NI...

journal_title：Biochemistry

authors： Potter LR

更新日期：1998-02-24 00:00:00

abstract：:Annexin V is a Ca(2+)-dependent, phospholipid-binding protein that may have one or more membrane-related functions. The binding of annexin V to phospholipids in a detergent micelle matrix was studied to attempt to determine directly the stoichiometry of specific phospholipid-binding sites and the importance of negativ...

journal_title：Biochemistry

authors： Meers P,Mealy T

更新日期：1993-11-02 00:00:00

abstract：:The transporter associated with antigen processing (TAP) is a prototype of an asymmetric ATP-binding cassette (ABC) transporter, which uses ATP binding and hydrolysis to translocate peptides from the cytosol to the lumen of the endoplasmic reticulum (ER). Here, we review molecular details of peptide binding and ATP bi...

journal_title：Biochemistry

authors： Hinz A,Tampé R

更新日期：2012-06-26 00:00:00

abstract：:Glycophorin, the MN blood group substance, is a major intrinsic glycoprotein in erythrocyte membranes. The interaction of glycophorin with phosphatidylcholine, 13C-labeled in specific positions in reconstituted unilamellar vesicles, was investigated by using the 13C NMR technique. 1-Palmitoyl-2-([14-13C]linoleoyl)-sn-...

journal_title：Biochemistry

authors： Utsumi H,Tunggal BD,Stoffel W

更新日期：1980-05-27 00:00:00

abstract：:We have established that UvrABC nuclease is equally efficient in cutting mitomycin C (MC)-DNA monoadducts formed at different sequences and that the degree of UvrABC cutting represents the extent of drug-DNA bonding. Using this method we determined the effect of C5 cytosine methylation on the DNA monoalkylation by MC ...

journal_title：Biochemistry

authors： Li VS,Reed M,Zheng Y,Kohn H,Tang M

更新日期：2000-03-14 00:00:00

abstract：:To elucidate the mechanism of solute diffusion through lipid bilayer membranes, nearly 4 ns of molecular dynamics simulations of solutes in phospholipid bilayers was conducted. The study, the first atomic level study of solute diffusion in a lipid bilayer, involved four simulations of an all-atom representation of a f...

journal_title：Biochemistry

authors： Bassolino-Klimas D,Alper HE,Stouch TR

更新日期：1993-11-30 00:00:00

abstract：:Myo1c is an unconventional myosin involved in cell signaling and membrane dynamics. Calcium binding to the regulatory-domain-associated calmodulin affects myo1c motor properties, but the kinetic details of this regulation are not fully understood. We performed actin gliding assays, ATPase measurements, fluorescence sp...

journal_title：Biochemistry

authors： Manceva S,Lin T,Pham H,Lewis JH,Goldman YE,Ostap EM

更新日期：2007-10-23 00:00:00

abstract：:The human endonuclease III (hNTH1) is an important DNA glycosylase with associated abasic lyase activity. We previously demonstrated that the K212Q mutant was totally inactive, while the K212R mutant had reduced DNA glycosylase/lyase activity and could form a covalent complex with the substrate DNA upon reduction. We ...

journal_title：Biochemistry

authors： Liu X,Roy R

更新日期：2001-11-13 00:00:00

abstract：:The objective of this work is to determine the molecular mechanism and regulation of short-chain acyl-CoA dehydrogenase (SCAD) from Megasphaera elsdenii. To achieve this, the gene coding for SCAD from M. elsdenii was cloned and sequenced. Site-directed mutagenesis was then used to identify an amino acid residue that i...

journal_title：Biochemistry

authors： Becker DF,Fuchs JA,Banfield DK,Funk WD,MacGillivray RT,Stankovich MT

更新日期：1993-10-12 00:00:00

abstract：:The nonbulky 5',8-cyclopurine DNA lesions (cP) and the bulky, benzo[ a]pyrene diol epoxide-derived stereoisomeric cis- and trans- N2-guanine adducts (BPDE-dG) are good substrates of the human nucleotide excision repair (NER) mechanism. These DNA lesions were embedded at the In or Out rotational settings near the dyad ...

journal_title：Biochemistry

authors： Shafirovich V,Kolbanovskiy M,Kropachev K,Liu Z,Cai Y,Terzidis MA,Masi A,Chatgilialoglu C,Amin S,Dadali A,Broyde S,Geacintov NE

更新日期：2019-02-12 00:00:00

abstract：:The interaction of oxidized nicotine adenine dinucleotide phosphate dependent isocitrate dehydrogenase (from pig heart) with (RS)-3-bromo-2-ketoglutarate was investigated in an effort to evaluate the reagent's potential as a selective reagent for alpha-ketoglutarate binding sites. The enzyme is rapidly inactivated by ...

journal_title：Biochemistry

authors： Hartman FC

更新日期：1981-02-17 00:00:00

abstract：:A 35-amino acid peptide corresponding to the putative "zinc finger" sequence of primase was prepared to study its zinc binding properties. When zinc was added to the peptide, it was found that the fluorescence quantum yield of the single tyrosine increased by 46% and the average lifetime by 34%. The binding stoichiome...

journal_title：Biochemistry

authors： Griep MA,Adkins BJ,Hromas D,Johnson S,Miller J

更新日期：1997-01-21 00:00:00

abstract：:The structures of four isomorphous crystals of ternary complexes of chicken heart citrate synthase with D- or L-malate and acetyl coenzyme A or carboxymethyl coenzyme A have been determined by X-ray crystallography and fully refined at 1.9-A resolution. The structures show that both L-malate and D-malate bind in a ver...

journal_title：Biochemistry

authors： Karpusas M,Holland D,Remington SJ

更新日期：1991-06-18 00:00:00

abstract：:RuvC is a well-characterized Holliday junction resolvase from E. coli. The presence of symmetry in its preferred recognition sequence leads to ambiguity in the position of the crossover point in the junction, because a symmetric junction can undergo branch migration. Symmetric immobile junctions are junctions that con...

journal_title：Biochemistry

authors： Sha R,Liu F,Iwasaki H,Seeman NC

更新日期：2002-09-10 00:00:00

abstract：:Apolipophorin III (apoLp-III) from the migratory locust, Locusta migratoria, represents the only full-length apolipoprotein whose three-dimensional structure has been solved. In the present study, spectroscopic methods have been employed to investigate the effects of deglycosylation (via endoglycosidase F treatment) a...

journal_title：Biochemistry

authors： Weers PM,Kay CM,Oikawa K,Wientzek M,Van der Horst DJ,Ryan RO

更新日期：1994-03-29 00:00:00

abstract：:The left-handed polyproline II (P(II)) helix is a structure that has been given a great deal of attention lately because of its role in a wide variety of physiologically important processes and potential significance in protein unfolded states. Recent work by several authors has shown that residues besides proline can...

journal_title：Biochemistry

authors： Chellgren BW,Creamer TP

更新日期：2004-05-18 00:00:00

abstract：:The dependence of calcineurin on Ca2+ for activity is the result of the concerted action of calmodulin, which increases the turnover rate of the enzyme and modulates its response to Ca2+ transients, and of calcineurin B, which decreases the Km of the enzyme for its substrate. The calmodulin-stimulated protein phosphat...

journal_title：Biochemistry

authors： Stemmer PM,Klee CB

更新日期：1994-06-07 00:00:00