Abstract:
:A Ca(2+)-activated thiol protease was purified from Drosophila melanogaster. The procedure involves Phenyl-Sepharose, Reactive Red-Agarose and Q-Sepharose fast flow (or MonoQ) chromatographic steps. The enzyme eluting from Q-Sepharose fast flow seems to be homogeneous as judged by silver staining on SDS-PAGE: it consists of a single polypeptide chain of M(r),app = 94K and pI = 5.46. The proteolytic activity of the purified enzyme is absolutely Ca(2+)-dependent, characterized by 0.6 mM free Ca2+ at half-maximal activity. Ca2+ ions cannot be replaced effectively by the divalent cations Mg2+, Mn2+, Zn2+, Ba2+, and Cd2+. The enzyme shows the inhibitor pattern of thiol proteases. Human recombinant calpastatin (domain I) completely inhibits the enzyme at a nearly 1:1 molar ratio. Several of these properties resemble those of vertebrate calpain II. However, various attempts to detect a small subunit of M(r) approximately 30K, common with vertebrate calpains, remained unsuccessful. We suggest that the Drosophila enzyme is a novel calpain II-like protease.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Pintér M,Stierandova A,Friedrich Pdoi
10.1021/bi00150a012subject
Has Abstractpub_date
1992-09-08 00:00:00pages
8201-6issue
35eissn
0006-2960issn
1520-4995journal_volume
31pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::c-Jun and c-Fos belong to the bZIP class of transcriptional activator proteins, many of which have been implicated in the neoplastic transformation of cells. We are interested in engineering dominant-negative leucine zipper (LZ) peptides as a means of sequestering these proteins in vivo in order to suppress their tran...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi952817o
更新日期:1996-07-16 00:00:00
abstract::Peptide nucleic acids (PNAs) are nonionic DNA/RNA mimics that can recognize complementary sequences by Watson-Crick base pairing. The neutral PNA backbone facilitates the recognition of duplex DNA by strand invasion, suggesting that antigene PNAs (agPNAs) can be important tools for exploring the structure and function...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi700230a
更新日期:2007-06-26 00:00:00
abstract::A time-resolved microscope fluorimeter based on a synchroscan streak camera and a fast pulsed laser system has been developed to measure the fluorescence lifetime decay under the fluorescence microscope. This system allows one to measure the nanosecond fluorescence lifetimes of fluorophores in a small spot (0.8-6.3 mi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00240a024
更新日期:1991-07-02 00:00:00
abstract::We introduce here a new model to describe the binding of extrinsic membrane proteins to acidic lipid membranes. In this view, macroscopic binding affinity is determined by two processes: nonspecific adsorption of protein to the membrane surface and association of acidic lipids with specific sites on the bound protein....
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00444a045
更新日期:1989-09-05 00:00:00
abstract::The S2 subsite specificity of the plant protease papain has been altered to resemble that of mammalian cathepsin B by site-directed mutagenesis. On the basis of amino acid sequence alignments for papain and cathepsin B, a double mutant (Val133Ala/Ser205Glu) was produced where Val133 and Ser205 are replaced by Ala and ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00101a003
更新日期:1991-09-17 00:00:00
abstract::Proteolytic fragments of bovine testis calmodulin were obtained by limited proteolysis with trypsin or thrombin. Cadmium-113 NMR studies showed that the tryptic fragment encompassing Ca2+ binding domains III and IV (TR2C) gave rise to a spectrum identical with that of the native protein. Two thrombic fragments contain...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00279a001
更新日期:1983-05-10 00:00:00
abstract::The membrane protein Rv0899 (OmpATb) from Mycobacterium tuberculosis, has been proposed to act as an outer membrane porin and to contribute to the bacterium's adaptation to the acidic environment of the phagosome during infection. The gene is restricted to pathogenic mycobacteria and, thus, is an attractive candidate ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi100158s
更新日期:2010-04-06 00:00:00
abstract::Purified subunits of intermediate filaments obtained from a variety of tissues and cell types contain O-phosphoserine and, in some cases, smaller amounts of O-phosphothreonine. The O-phosphoserine content was estimated by reaction of performic acid oxidized subunits with methylamine in NaOH. Decamin of BHK-21 and CHO ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00530a030
更新日期:1982-01-05 00:00:00
abstract::Immunological methods, in parellel with measurement of ATPase activity, have been used to characterize the reactions of antibodies specific for light chains with myosin and its water-soluble proteolytic subfragments, heavy meromyosin (HMM) and subfragment 1 (HMM S-1). Antiserum to the 5,5'-dithiobis(2-nitro-enzoic aci...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00692a008
更新日期:1975-10-21 00:00:00
abstract::Synthetic nonviral vector systems are attractive because of their apparent simplicity of preparation and use. However, there are many barriers to success at the moment, including the formulation of uniform and reproducible particles of transfection competent, condensed nucleic acids such as plasmid DNA (pDNA). For thi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034325e
更新日期:2003-09-09 00:00:00
abstract::New analogues of 7-methylguanosine 5'-monophosphate (m7GMP) were synthesized with modified 5'-phosphate moieties by replacement of -O with -H, -CH3, or -NH2. Additional analogues were synthesized with 8-methyl- or 8-aminoguanine base substitutions or ring-opened ribose (2',3'-diol). These compounds were analyzed by 1H...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00388a028
更新日期:1987-07-14 00:00:00
abstract::Benzoylformate decarboxylase (BFDC) and pyruvate decarboxylase (PDC) are both thiamin diphosphate-dependent enzymes. The two share a common three-dimensional structure and catalyze a similar chemical reaction, i.e., decarboxylation of 2-keto acids. However, they vary significantly in their substrate utilization patter...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9008402
更新日期:2009-09-08 00:00:00
abstract::S100 beta is a calcium-binding protein, which regulates the activities of several enzymes and inhibits the phosphorylation of a variety of protein kinase C substrates in a calcium-dependent manner. The protein was recently found to activate a retinal membrane guanylate cyclase, and in this paper, we report that it inh...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9802115
更新日期:1998-07-28 00:00:00
abstract::The first step of the kynurenine pathway for l-tryptophan (l-Trp) degradation is catalyzed by heme-dependent dioxygenases, tryptophan 2,3-dioxygenase (TDO) and indoleamine 2,3-dioxygenase. In this work, we employed stopped-flow optical absorption spectroscopy to study the kinetic behavior of the Michaelis complex of C...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.0c00179
更新日期:2020-08-04 00:00:00
abstract::Previous studies have shown that protein 4.1 is a multifunctional protein that binds to spectrin, actin, glycophorins, the anion channel protein, and phosphatidylserine (PS). In this report, we have characterized the binding of protein 4.1 and its major proteolytic fragments to phospholipid vesicles. Pure 125I-labeled...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00402a018
更新日期:1988-01-26 00:00:00
abstract::The primary subsite specificities of human leukocyte elastase, cathepsin G, porcine pancreatic elastase, rat mast cell proteases I and II, bovine chymotrypsin A alpha, and the protease from strain V-8 of Staphylococcus aureus have been mapped with a series of tripeptide thiobenzyl ester substrates of the general formu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00308a023
更新日期:1984-06-19 00:00:00
abstract::At any instant, the human erythrocyte sugar transporter presents at least one sugar export site but multiple sugar import sites. The present study asks whether the transporter also presents more than one sugar exit site. We approached this question by analysis of binding of [3H]cytochalasin B (an export conformer liga...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi015586w
更新日期:2001-12-25 00:00:00
abstract::Cyclopentenone prostanoids (cyP) arise as important modulators of inflammation and cell proliferation. Although their physiological significance has not been fully elucidated, their potent biological effects have spurred their study as leads for the development of therapeutic agents. A key determinant of cyP action is...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi602389p
更新日期:2007-06-05 00:00:00
abstract::Both metal and flavin-dependent sulfhydryl oxidases catalyze the net generation of disulfide bonds with the reduction of oxygen to hydrogen peroxide. The first mammalian sulfhydryl oxidase to be described was an iron-dependent enzyme isolated from bovine milk whey (Janolino, V.G., and Swaisgood, H.E. (1975) J. Biol. C...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi7016975
更新日期:2007-11-13 00:00:00
abstract::We extended the technique of frequency-domain fluorometry to an upper frequency limit of 2000 MHz. This was accomplished by using the harmonic content of a laser pulse train (3.76 MHz, 5 ps) from a synchronously pumped and cavity-dumped dye laser. We used a microchannel plate photomultiplier as the detector to obtain ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00375a012
更新日期:1987-01-13 00:00:00
abstract::GTPases are key players during cellular signaling. Phosphorylation of Rab proteins, which belong to the Ras superfamily of small GTPases regulating intracellular transport, has been implicated in the pathogenesis of Parkinson's disease. For Rab8a, it was shown that serine 111 phosphorylation (pS111) is dependent on th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.9b00516
更新日期:2019-08-20 00:00:00
abstract::The role of the 2'-hydroxyl group in RNA--protein interaction has been investigated using MS2 coat protein and its hairpin RNA operator as a model system. Derivatives of the MS2 translational operator were prepared where individual riboses were replaced by deoxyribose and their binding affinities to MS2 coat protein w...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00038a033
更新日期:1995-09-26 00:00:00
abstract::Tandem DNA lesions containing two contiguously damaged nucleotides are commonly formed by ionizing radiation. Their effects on replication in mammalian cells are largely unknown. Replication of isolated 2-deoxyribonolactone (L), thymine glycol (Tg), and tandem lesion 5'-LTg was examined in human cells. Although nearly...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.9b01058
更新日期:2020-02-04 00:00:00
abstract::Using lattice statistical mechanics, we develop theory to account for the folding of a heteropolymer molecule such as a protein to the globular and soluble state. Folding is assumed to be driven by the association of solvophobic monomers to avoid solvent and opposed by the chain configurational entropy. Theory predict...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00327a032
更新日期:1985-03-12 00:00:00
abstract::Transient phosphorylation at an aspartate residue on the Spo0F protein is a central step in the phosphorelay signal transduction pathway controlling sporulation in Bacilli. The response regulator Spo0F-P is stable to hydrolysis (t1/2 > 24 h at 23 degrees C in the absence of Mg2+), allowing the use of nondenaturing PAG...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9519361
更新日期:1996-03-05 00:00:00
abstract::PLK1 (polo-like kinase 1) is a key mitotic kinase and a therapeutic target in the treatment of proliferative diseases. Here we investigate the relative substrate specificity and pharmacological relatedness of PLK1, -2, -3, and -4 that together comprise a conserved family of Ser/Thr kinases (PLK family). We report cons...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi7008745
更新日期:2007-08-21 00:00:00
abstract::PDZ domains are among the most abundant protein modules in the known genomes. Their main function is to provide scaffolds for membrane-associated protein complexes by binding to the cytosolic, C-terminal fragments of receptors, channels, and other integral membrane proteins. Here, using both heteronuclear NMR and sing...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi052225y
更新日期:2006-03-21 00:00:00
abstract::Glucocorticoid (GC) hormones induce apoptosis in T-cell and pre-B-cell acute lymphoblastic leukemia (ALL) cells. Steroid-mediated apoptosis requires a threshold level of the glucocorticoid receptor (GR) protein, and increasing the intracellular GR levels in ALL cells would augment their hormone sensitivity. A protein ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi1007723
更新日期:2010-10-19 00:00:00
abstract::The electron spin echo decay envelope for the blue copper protein, stellacyanin, and for a number of other Cu(II) complexes has been studied. Particular attention was given to the form of the "nuclear modulation" patterns, which show the effects of coupling between the electron spin and the neighboring nuclei. The env...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00662a033
更新日期:1976-08-24 00:00:00
abstract::Prolyl endopeptidase (Prep) is a member of the prolyl peptidase family and is of interest because of its unique biochemistry and connections to cognitive function. Using an unbiased mass spectrometry (MS)-based peptidomics platform, we identified Prep-regulated peptides in the central nervous system (CNS) of mice by m...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi901637c
更新日期:2009-12-22 00:00:00