Thermal behavior of stearoylsphingomyelin-cholesterol dispersions.

abstract：:We examine the effect of cargo-motor linkage stiffness on the mechanobiological properties of the molecular motor myosin VI. We use the programmability of DNA nanostructures to modulate cargo-motor linkage stiffness and combine it with high-precision optical trapping measurements to measure the effect of linkage stiff...

journal_title：Biochemistry

authors： Shrivastava R,Rai A,Salapaka M,Sivaramakrishnan S

更新日期：2019-11-26 00:00:00

abstract：:Helicobacter pylori NikR (HpNikR) is a nickel-responsive transcription factor. In addition to a role in nickel homeostasis, HpNikR is proposed to serve as a master activator-repressor for H. pylori acid adaptation by directly or indirectly regulating the expression of a battery of genes. One potential mechanism of thi...

journal_title：Biochemistry

authors： Li Y,Zamble DB

更新日期：2009-03-24 00:00:00

abstract：:Human P-glycoprotein (Pgp), a plasma membrane protein that confers multidrug resistance, functions as an ATP-dependent drug efflux pump. Pgp contains two ATP binding/utilization sites and exhibits ATPase activity that is stimulated in the presence of substrates and modulating agents. The mechanism of coupling of ATP h...

journal_title：Biochemistry

authors： Ramachandra M,Ambudkar SV,Chen D,Hrycyna CA,Dey S,Gottesman MM,Pastan I

更新日期：1998-04-07 00:00:00

abstract：:The interaction of novel, tetrafunctional bisplatinum compounds with DNA was investigated. These compounds have bis(vicinal 1,2-diamines) as ligand. The reactions' efficiency, types of cross-links, alterations of the global DNA structure, and sequence selectivity differ significantly from the corresponding features of...

journal_title：Biochemistry

authors： Büning H,Altman J,Beck W,Zorbas H

更新日期：1997-09-23 00:00:00

abstract：:We have previously shown that the anticodon of methionine tRNAs contains most, if not all, of the nucleotides required for specific recognition of tRNA substrates by Escherichia coli methionyl-tRNA synthetase [Schulman, L. H., & Pelka, H. (1988) Science 242, 765-768]. Previous cross-linking experiments have also ident...

journal_title：Biochemistry

authors： Ghosh G,Pelka H,Schulman LH

更新日期：1990-03-06 00:00:00

abstract：:Circumsporozoite (CS) protein is a predominant surface antigen of malaria sporozoites, the infective form of the parasite, and has been used for making anti-malaria vaccines. For the first time we have examined the interaction of CS protein with various glycosaminoglycans in real time using surface plasmon resonance (...

journal_title：Biochemistry

authors： Rathore D,McCutchan TF,Garboczi DN,Toida T,Hernáiz MJ,LeBrun LA,Lang SC,Linhardt RJ

更新日期：2001-09-25 00:00:00

abstract：:In an attempt to understand the multifunctional involvement of beta(2)-glycoprotein I (beta(2)GPI) in autoimmune diseases, thrombosis, atherosclerosis, and inflammatory processes, substantial interest is focused on the interaction of beta(2)GPI with negatively charged ligands, in particular, with acidic phospholipids....

journal_title：Biochemistry

authors： Hammel M,Schwarzenbacher R,Gries A,Kostner GM,Laggner P,Prassl R

更新日期：2001-11-27 00:00:00

abstract：:Allosteric activators are generally believed to shift the equilibrium distribution of enzyme conformations to favor a catalytically productive structure; the kinetics of conformational exchange is seldom addressed. Several observations suggested that the usual allosteric mechanism might not apply to the activation of ...

journal_title：Biochemistry

authors： Riera TV,Zheng L,Josephine HR,Min D,Yang W,Hedstrom L

更新日期：2011-10-04 00:00:00

abstract：:Hepatic microsomal NADPH-cytochrome P-450 reductase was solubilized from rabbit liver microsomes in the presence of detergents and purified to homogeneity by column chromatography. The purified reductase had a molecular weight of 78 000 and contained 1 mol each of FAD and FMN per mol of enzyme. On reduction with NADPH...

journal_title：Biochemistry

authors： Iyanagi T,Anan FK,Imai Y,Mason HS

更新日期：1978-05-30 00:00:00

abstract：:Significant concentrations (1-10 mM) of nickel(II) were found in solution after incubation of the potent carcinogen nickel subsulfide in 0.05 M Tris-HCl, pH 7.4, solutions containing DNA, rat liver microsomes, and NADPH. The presence of NADPH decreased the rate of solubilization of nickel subsulfide. The solubilized n...

journal_title：Biochemistry

authors： Lee JE,Ciccarelli RB,Jennette KW

更新日期：1982-02-16 00:00:00

abstract：:Trypanosomes are purine-auxotrophic parasites that depend upon nucleoside hydrolase (NH) activity to salvage nitrogenous bases necessary for nucleic acid and cofactor synthesis. Nonspecific and purine-specific NHs have been widely studied, yet little is known about the 6-oxopurine-specific isozymes, although they are ...

journal_title：Biochemistry

authors： Vandemeulebroucke A,Minici C,Bruno I,Muzzolini L,Tornaghi P,Parkin DW,Versées W,Steyaert J,Degano M

更新日期：2010-10-19 00:00:00

abstract：:The N-terminus of the major polypeptide component of the light-harvesting complex (LHC) from the brown alga Laminaria saccharina is blocked. Two partial sequences, one near the N-terminus and the other near the C-terminus, have been obtained by chemical cleavage with acetic acid and N-chlorosuccinimide. Four peptides ...

journal_title：Biochemistry

authors： Douady D,Rousseau B,Caron L

更新日期：1994-03-22 00:00:00

abstract：:The roles of four of the active center groups with potential acid-base properties in the region of pH optimum of pyruvate decarboxylase from Saccharomyces cerevisiae have been studied with the substitutions Asp28Ala, His114Phe, His115Phe, and Glu477Gln, introduced by site-directed mutagenesis methods. The steady-state...

journal_title：Biochemistry

authors： Liu M,Sergienko EA,Guo F,Wang J,Tittmann K,Hübner G,Furey W,Jordan F

更新日期：2001-06-26 00:00:00

abstract：:The complete sequence of rabbit antibody light-chain L 120 has been elucidated. The antibody was raised against Micrococcus lysodeikticus bacteria and is specific for the external part of the cell wall. All protein used in this work was obtained from a single 50-mL bleeding. The variable region of L 120 is compared to...

journal_title：Biochemistry

authors： Van Hoegaerden M,Strosberg AD

更新日期：1978-10-03 00:00:00

abstract：:1-Deoxy-D-fructose was synthesized in 27% yield from D-glucosamine in a three-step procedure involving Raney nickel desulfurization and oxidative deamination with 3,5-di-tert-butyl- 1,2-benzoquinone applied to appropriate intermediates. 1-Deoxyfructose and its reduction products, 1-deoxyglucitol and 1-deoxymannitol, w...

journal_title：Biochemistry

authors： Dills WL Jr,Meyer WL

更新日期：1976-10-05 00:00:00

abstract：:Putative domain--domain interactions of the monomeric bifunctional enzyme indoleglycerol phosphate synthase:phosphoribosyl anthranilate isomerase from Escherichia coli were probed by separating the domains on the gene level and expressing them as monofunctional proteins. The engineered monofunctional enzymes were foun...

journal_title：Biochemistry

authors： Eberhard M,Tsai-Pflugfelder M,Bolewska K,Hommel U,Kirschner K

更新日期：1995-04-25 00:00:00

abstract：:At pH 5.5, binding of bleomycin relaxed supercoiled ColE1 DNA without breaking it. Binding of tripeptide S, a fragment of the drug containing the bithiazole rings, also relaxed and then recoiled supercoiled DNA, at pH 5.5 and at pH 8.0, where bleomycin is normally active. The unwinding angle was 12 degrees. Both compo...

journal_title：Biochemistry

authors： Povirk LF,Hogan M,Dattagupta N

更新日期：1979-01-09 00:00:00

abstract：:The empoyment of a novel method of affinity chromatography, which makes use of antibodies that specifically bind DNA/RNA hybrids, has made it possible to enrich for rat rDNA molecules which contain R loops formed with the 18S and 28S rRNAs. An approximately 150-fold enrichment of the ratrRNA coding sequences was obtai...

journal_title：Biochemistry

authors： Stumph WE,Wu JR,Bonner J

更新日期：1979-06-26 00:00:00

abstract：:A series of 24-homologated 1,25-dihydroxyvitamin D3 compounds have been chemically synthesized and studied with regard to their activity in inducing differentiation of human promyelocyte HL-60 cells to monocytes and in calcium mobilizing activity in vitamin D deficient rats. Homologation of 1,25-dihydroxyvitamin D3 or...

journal_title：Biochemistry

authors： Perlman K,Kutner A,Prahl J,Smith C,Inaba M,Schnoes HK,DeLuca HF

更新日期：1990-01-09 00:00:00

abstract：:We recently developed a new, rapid, and specific bioassay system that employs a fluorescent probe fabricated from our discovered CXCR4-specific ligand DV1. This new probe sensitively and selectively blocks the binding of native and synthetic ligands to CXCR4 at nanomolar levels, with a capability comparable to that se...

journal_title：Biochemistry

authors： Yang Y,Zhang Q,Gao M,Yang X,Huang Z,An J

更新日期：2014-08-05 00:00:00

abstract：:To study the role of the N-terminal region of the estrogen receptor (ER) in transcription activation and in DNA binding, we constructed a mutant of the Xenopus laevis ER which lacks amino acids 1-159 (XER160/586). In transient transfections, XER160/586 exhibited < 10% of the activity of wild-type XER on a synthetic pr...

journal_title：Biochemistry

authors： Xing H,Mattick S,Lew D,Shapiro DJ

更新日期：1995-03-28 00:00:00

abstract：:A newly recognized type of protein-ligand interaction phenomenon has resulted in the preparation of simple, nonionic, and highly specific gel derivatives for selective adsorption chromatography. The essential structure of the immobilized ligand can be represented as agarose-CH2CH2SO2CH2CH2SCH2CH2OH, which was prepared...

journal_title：Biochemistry

authors： Hutchens TW,Porath J

更新日期：1987-11-03 00:00:00

abstract：:Hydroxyl radical induced formation of a DNA-protein cross-link involving thymine and tyrosine in nucleohistone is described. Hydroxyl radicals were generated in N2O-saturated aqueous solution by ionizing radiation. Samples of nucleohistone were hydrolyzed with HCl and trimethylsilylated. Analysis of irradiated samples...

journal_title：Biochemistry

authors： Dizdaroglu M,Gajewski E,Reddy P,Margolis SA

更新日期：1989-04-18 00:00:00

abstract：:The interaction of poly(N epsilon, N epsilon, N epsilon-trimethyl-L-lysine) ([Lys(Me3)]n) and poly(N delta, N delta, N delta-trimethyl-L-ornithine) ([Orn(Me3)]n) with polynucleotides was studied by thermal denaturation, viscosity, and dissociation by salt. The methylated polymers decrease the viscosity of DNA in propo...

journal_title：Biochemistry

authors： Granados EN,Bello J

更新日期：1980-07-08 00:00:00

abstract：:Trypanosoma and Leishmania, pathogens responsible for diseases such as African sleeping sickness, Chagas' heart disease, or Oriental sore, are two of the very few genera that do not use the ubiquitous glutathione/glutathione reductase system to keep a stable cellular redox balance. Instead, they rely on trypanothione ...

journal_title：Biochemistry

authors： Stoll VS,Simpson SJ,Krauth-Siegel RL,Walsh CT,Pai EF

更新日期：1997-05-27 00:00:00

abstract：:The kinetics of the interconversions of the subgel and gel phases in dipalmitoylphosphatidylcholine have been studied by using differential dilatometry, differential scanning calorimetry (DSC), and neutral buoyancy centrifugation as a function of incubation temperature and deuteriation of the solvent. As seen by other...

journal_title：Biochemistry

authors： Tristram-Nagle S,Wiener MC,Yang CP,Nagle JF

更新日期：1987-07-14 00:00:00

abstract：:The Wiskott-Aldrich syndrome protein (WASP) and neural WASP (N-WASP) are key players in regulating actin cytoskeleton via the Arp2/3 complex. It has been widely reported that the WASP proteins are activated by Rho family small GTPase Cdc42 and that Rac1 acts through SCAR/WAVE proteins. However, a systematic study of t...

journal_title：Biochemistry

authors： Tomasevic N,Jia Z,Russell A,Fujii T,Hartman JJ,Clancy S,Wang M,Beraud C,Wood KW,Sakowicz R

更新日期：2007-03-20 00:00:00

abstract：:In bee venom phospholipase A2, histidine-34 probably functions as a Brønsted base to deprotonate the attacking water. Aspartate-64 and tyrosine-87 form a hydrogen bonding network with histidine-34. We have prepared mutants at these positions and studied their kinetic properties. The mutant in which histidine-34 is cha...

journal_title：Biochemistry

authors： Annand RR,Kontoyianni M,Penzotti JE,Dudler T,Lybrand TP,Gelb MH

更新日期：1996-04-09 00:00:00

abstract：:Murine class alpha glutathione S-transferase subunit types A2 (mGSTA2-2) and A1 (mGSTA1-1) have high catalytic efficiency for glutathione (GSH) conjugation of the ultimate carcinogenic metabolite of benzo[a]pyrene, (+)-anti-7,8-dihydroxy-9,10-oxy-7,8,9,10-tetrahydrobenzo[a]pyrene, [(+)-anti-BPDE]. Only 10 residues dif...

journal_title：Biochemistry

authors： Gu Y,Xiao B,Wargo HL,Bucher MH,Singh SV,Ji X

更新日期：2003-02-04 00:00:00

abstract：:Calpain-catalyzed proteolysis of II-spectrin is a regulated event associated with neuronal long-term potentiation, platelet and leukocyte activation, and other processes. Calpain proteolysis is also linked to apoptotic and nonapoptotic cell death following excessive glutamate exposure, hypoxia, HIV-gp120/160 exposure,...

journal_title：Biochemistry

authors： Glantz SB,Cianci CD,Iyer R,Pradhan D,Wang KK,Morrow JS

更新日期：2007-01-16 00:00:00