eIF2B Mechanisms of Action and Regulation: A Thermodynamic View.

abstract：:ATP hydrolysis by the maltose transporter (MalFGK(2)) is regulated by maltose binding protein (MBP). Binding of maltose to MBP brings about a conformational change from open to closed that leads to a strong stimulation of the MalFGK(2) ATPase. In this study, we address the long-standing but enigmatic observation that ...

journal_title：Biochemistry

authors： Gould AD,Telmer PG,Shilton BH

更新日期：2009-08-25 00:00:00

abstract：:O-Aryloxycarbonyl hydroxamates have previously been shown to efficiently inactivate class C β-lactamases by cross-linking serine and lysine residues in the active site. A new analogue of these inhibitors, D-(R)-O-(phenoxycarbonyl)-N-[(4-amino-4-carboxy-1-butyl)oxycarbonyl]hydroxylamine, designed to inactivate certain ...

journal_title：Biochemistry

authors： Tilvawala R,Cammarata M,Adediran SA,Brodbelt JS,Pratt RF

更新日期：2015-12-22 00:00:00

abstract：:We investigated the molecular mechanisms and the binding site location for the fluorophor crystal violet (CrV), a noncompetitive antagonist of the nicotinic acetylcholine receptor (AChR). To this end, radiolabeled competition binding, fluorescence spectroscopy, Schild-type analysis, patch-clamp recordings, and molecul...

journal_title：Biochemistry

authors： Arias HR,Bhumireddy P,Spitzmaul G,Trudell JR,Bouzat C

更新日期：2006-02-21 00:00:00

abstract：:The ribonucleoprotein ribonuclease P (RNase P) cleaves all tRNA precursors endonucleolitically to produce the mature 5'-end. Dictyostelium discoideum RNase P displays an absolute requirement for Mg2+. Only the alkaline earth cations Ca2+, Sr2+, and Ba2+, under appropriate conditions can substitute to some extent for M...

journal_title：Biochemistry

authors： Tekos A,Stathopoulos C,Drainas D

更新日期：1998-11-03 00:00:00

abstract：:Human telomeric DNA consists of tandem repeats of the DNA sequence d(GGGTTA). Oligodeoxynucleotide telomere models such as d[A(GGGTTA)(3)GGG] (Tel22) fold in a cation-dependent manner into quadruplex structures consisting of stacked G-quartets linked by d(TTA) loops. NMR has shown that in Na(+) solutions Tel22 forms a...

journal_title：Biochemistry

authors： Gray RD,Petraccone L,Trent JO,Chaires JB

更新日期：2010-01-12 00:00:00

abstract：:Ammonia binds directly to the oxygen-evolving complex of photosystem II (PSII) upon formation of the S2 intermediate, as evidenced by electron paramagnetic resonance spectroscopy. We explore the binding mode by using quantum mechanics/molecular mechanics methods and simulations of extended X-ray absorption fine struct...

journal_title：Biochemistry

authors： Askerka M,Vinyard DJ,Brudvig GW,Batista VS

更新日期：2015-09-29 00:00:00

abstract：:Human prolactin (hPRL) binds two human prolactin receptor molecules, creating active heterotrimeric complexes. Receptors bind dissimilar hormone surfaces termed site 1 and site 2 in an obligate ordered process. We sought to map the functional epitopes in site 1 of hPRL. Extensive alanine mutagenesis (102 of the 199 re...

journal_title：Biochemistry

authors： Rao GV,Brooks CL

更新日期：2011-03-01 00:00:00

abstract：:Glycophorin, the MN blood group substance, is a major intrinsic glycoprotein in erythrocyte membranes. The interaction of glycophorin with phosphatidylcholine, 13C-labeled in specific positions in reconstituted unilamellar vesicles, was investigated by using the 13C NMR technique. 1-Palmitoyl-2-([14-13C]linoleoyl)-sn-...

journal_title：Biochemistry

authors： Utsumi H,Tunggal BD,Stoffel W

更新日期：1980-05-27 00:00:00

abstract：:Tandem DNA lesions containing two contiguously damaged nucleotides are commonly formed by ionizing radiation. Their effects on replication in mammalian cells are largely unknown. Replication of isolated 2-deoxyribonolactone (L), thymine glycol (Tg), and tandem lesion 5'-LTg was examined in human cells. Although nearly...

journal_title：Biochemistry

authors： Naldiga S,Huang H,Greenberg MM,Basu AK

更新日期：2020-02-04 00:00:00

abstract：:The Pf1 gene 5 protein forms a large helical nucleoprotein complex (Mr = 3.1 x 10(7)) with single-stranded viral DNA, from which a 32 amino acid sequence rich in alanine, proline, and glutamine residues can be removed from the C-terminus by limited proteolysis. Sharp resonances in the 1H NMR spectrum of the Pf1 nucleo...

journal_title：Biochemistry

authors： Davis KG,Plyte SE,Robertson SR,Cooper A,Kneale GG

更新日期：1995-01-10 00:00:00

abstract：:The membrane receptor for immunoglobulin E (IgE) and its ligand, IgE, were irradiated with high-energy electrons. Loss of binding activity was measured for each, and the size of the functional targets was assessed. In both cases, the target size was substantially smaller than the covalent structure of the molecule. Th...

journal_title：Biochemistry

authors： Fewtrell C,Kempner E,Poy G,Metzger H

更新日期：1981-11-10 00:00:00

abstract：:The fluorescence of Eu(III) is used to study the nature of the Ca(II) binding sites in the central cavity of the two-zinc(II) insulin hexamer. The dependence of the Eu(III) fluorescence lifetime upon Eu(III) stoichiometry indicates that there are three identical Eu(III) binding sites present in the two-zinc(II) insuli...

journal_title：Biochemistry

authors： Alameda GK,Evelhoch JL,Sudmeier JL,Birge RR

更新日期：1985-03-26 00:00:00

abstract：:The effect of DNA supercoiling on a sterically very demanding threading intercalation process is investigated here. We find that the threading rate of a dimeric ruthenium complex into a negatively supercoiled plasmid at low binding density is 2 orders of magnitude higher than into the cleaved linear form. Further satu...

journal_title：Biochemistry

authors： Nordell P,Jansson ET,Lincoln P

更新日期：2009-02-24 00:00:00

abstract：:Native calf thymus DNA was reacted with N-acetoxy-N-2-acetylaminofluorene (N-AcO-AAF) and its 7-fluoro and 7-iodo derivatives. Different ways of purification of the fluorene modified DNA samples were checked in order ot obtain a nucleic acid free from all noncovalently bound fluorene residues. The decrease in melting ...

journal_title：Biochemistry

authors： Fuchs RP,Lefevre JF,Pouyet J,Daune MP

更新日期：1976-07-27 00:00:00

abstract：:The 270-MHz 1H nuclear magnetic resonance spectra of the apo, copper(I)-zinc(II), and copper(II)-zinc(II) forms of bovine erythrocyte superoxide dismutase (EC 1.15.1.1) are reported, and assignments of resonances to ten amino acid residues are proposed. The data require that at least four and probably six histidine re...

journal_title：Biochemistry

authors： Cass AE,Hill AO,Smith BE,Bannister JV,Bannister WH

更新日期：1977-07-12 00:00:00

abstract：:Lysyl-5-fluoro-L-tryptophyllysine and lysyl-5-fluoro-D-tryptophyllysine were synthesized, and their interactions with double-stranded DNA were investigated as a model for protein-nucleic acid interactions. The binding to DNA was studied by monitoring various 19F NMR parameters, the fluorescence, and the optical absorb...

journal_title：Biochemistry

authors： Shine NR,James TL

更新日期：1985-07-30 00:00:00

abstract：:We have investigated the interaction of the bisbenzimidazole derivative Hoechst 33258 with the self-complementary dodecadeoxynucleotide duplex d(GTGGAATTCCAC)2 using one-dimensional (1D) and two-dimensional (2D) proton nuclear magnetic resonance (1H NMR) spectroscopy. To monitor the extent of complex formation, we use...

journal_title：Biochemistry

authors： Fede A,Labhardt A,Bannwarth W,Leupin W

更新日期：1991-12-03 00:00:00

abstract：:Ran, a small nuclear GTP-binding protein, is one of the most abundant Ras-related proteins in eucaryotic cells. Ran is essential for nucleo-cytoplasmatic transport and is primarily localized in the nucleus and at the nuclear pore complex. Here, we characterize the kinetics and equilibrium of the interaction between Ra...

journal_title：Biochemistry

authors： Kuhlmann J,Macara I,Wittinghofer A

更新日期：1997-10-07 00:00:00

abstract：:Typical cytochrome P450s secure the heme prosthetic group with a cysteine thiolate ligand bound to the iron, electrostatic interactions with the heme propionate carboxylates, and hydrophobic interactions with the heme periphery. In addition to these interactions, CYP4B1 covalently binds heme through a monoester link f...

journal_title：Biochemistry

authors： Baer BR,Schuman JT,Campbell AP,Cheesman MJ,Nakano M,Moguilevsky N,Kunze KL,Rettie AE

更新日期：2005-10-25 00:00:00

abstract：:Phosphite dehydrogenase (PTDH) is a unique NAD-dependent enzyme that catalyzes the oxidation of inorganic phosphite to phosphate. The enzyme has great potential for cofactor regeneration, and mechanistic studies have provided some insight into the residues that are important for catalysis. In this investigation, pre-s...

journal_title：Biochemistry

authors： Fogle EJ,van der Donk WA

更新日期：2007-11-13 00:00:00

abstract：:In a large number of plant species hydroxynitrile lyases catalyze the decomposition of cyanohydrins in order to generate hydrogen cyanide upon tissue damage. Hydrogen cyanide serves as a deterrent against herbivores and fungi. In vitro hydroxynitrile lyases are proficient biocatalysts for the stereospecific synthesis ...

journal_title：Biochemistry

authors： Dreveny I,Andryushkova AS,Glieder A,Gruber K,Kratky C

更新日期：2009-04-21 00:00:00

abstract：:Computer graphics and energy minimization techniques were used to construct a model of DNA containing cis-thymine glycol, an oxidation product of thymine formed in DNA by ionizing radiation. The model simulated an experimental DNA substrate used to study the effects of this lesion on DNA synthesis in vitro. The result...

journal_title：Biochemistry

authors： Clark JM,Pattabiraman N,Jarvis W,Beardsley GP

更新日期：1987-08-25 00:00:00

abstract：:M proteins and other members of the M protein family, expressed on the surface of Streptococcus pyogenes, bind host proteins such as immunoglobulins, albumin, and fibrinogen. Protein H and the M1 protein are expressed by adjacent genes and both belong to the M protein family. In this work, the structure and stability ...

journal_title：Biochemistry

authors： Nilson BH,Frick IM,Akesson P,Forsén S,Björck L,Akerström B,Wikström M

更新日期：1995-10-17 00:00:00

abstract：:Bovine liver rhodanese, which catalyzes the transfer of sulfur atoms between a variety of sulfur donor and sulfur acceptor substrates, is inhibited by metal cyanide complexes [Volini, M., Van Sweringen, B., & Chen, F.-Sh. (1978) Arch. Biochem. Biophys. 191, 205-215]. Crystallographic studies are described which reveal...

journal_title：Biochemistry

authors： Lijk LJ,Kalk KH,Brandenburg NP,Hol WG

更新日期：1983-06-07 00:00:00

abstract：:A heterologous expression system was used to evaluate activation of BlaR1, a sensor/signal transducer protein of Staphylococcus aureus with a central role in resistance to β-lactam antibiotics. In the absence of other S. aureus proteins that might respond to antibiotics and participate in signal transduction events, w...

journal_title：Biochemistry

authors： Llarrull LI,Mobashery S

更新日期：2012-06-12 00:00:00

abstract：:ISU-type proteins mediate cluster transfer to apo protein targets. Rate constants have been determined for cluster transfer from ISU to apo Fd for both Homo sapiens and Schizosaccharomyces pombe proteins, and cross reactions have also been examined. Substitution of a key aspartate residue of ISU is found to decrease t...

journal_title：Biochemistry

authors： Wu SP,Wu G,Surerus KK,Cowan JA

更新日期：2002-07-16 00:00:00

abstract：:Translesion synthesis (TLS) is a mutagenic branch of cellular DNA damage tolerance that enables bypass replication over DNA lesions carried out by specialized low-fidelity DNA polymerases. The replicative bypass of most types of DNA damage is performed in a two-step process of Rev1/Polζ-dependent TLS. In the first ste...

journal_title：Biochemistry

authors： Pustovalova Y,Magalhães MT,D'Souza S,Rizzo AA,Korza G,Walker GC,Korzhnev DM

更新日期：2016-04-05 00:00:00

abstract：:Our previous studies demonstrated that the first 1000 amino acid residues (the βα1 domain) of human apolipoprotein (apo) B-100, termed apoB:1000, are required for the initiation of lipoprotein assembly and the formation of a monodisperse stable phospholipid (PL)-rich particle. The objectives of this study were (a) to ...

journal_title：Biochemistry

authors： Manchekar M,Kapil R,Sun Z,Segrest JP,Dashti N

更新日期：2017-08-08 00:00:00

abstract：:The ATP analogue 5'-[p-(fluorosulfonyl)benzoyl]adenosine (FSBA) was used to chemically modify the ATP binding sites of the carbamyl phosphate synthetase domain of CAD, the multifunctional protein that catalyzes the first steps in mammalian pyrimidine biosynthesis. Reaction of CAD with FSBA resulted in the inactivation...

journal_title：Biochemistry

authors： Kim HS,Lee L,Evans DR

更新日期：1991-10-22 00:00:00

abstract：:The origin of the nuclear magnetic resonance (NMR)-measurable ATP in equilibrium Pi exchange and whether it can be used to determine net oxidative ATP synthesis rates in the intact myocardium were examined by detailed measurements of ATP in equilibrium Pi exchange rates in both directions as a function of the myocardi...

journal_title：Biochemistry

authors： Kingsley-Hickman PB,Sako EY,Mohanakrishnan P,Robitaille PM,From AH,Foker JE,Uğurbil K

更新日期：1987-11-17 00:00:00