Abstract:
:The fluorescence of Eu(III) is used to study the nature of the Ca(II) binding sites in the central cavity of the two-zinc(II) insulin hexamer. The dependence of the Eu(III) fluorescence lifetime upon Eu(III) stoichiometry indicates that there are three identical Eu(III) binding sites present in the two-zinc(II) insulin hexamer in solution. Addition of excess Ca(II) causes a decrease in the Eu(III) fluorescence intensity, confirming that Ca(II) competes for the observed Eu(III) sites. The solvent dependence of the Eu(III) fluorescence lifetime (H2O vs. D2O) indicates that four OH groups are coordinated to each Eu(III) in the hexamer. Substitution of Co(II) for Zn(II) causes a decrease in the Eu(III) fluorescence lifetime. Calculations based on Förster energy-transfer theory predict that the Co(II) [or Zn(II) in vivo] and Eu(III) [or Ca(II) in vivo] binding sites are separated by 9.6 +/- 0.5 A. Variation of the metal stoichiometries indicates that all three Eu(III) [or Ca(II) in vivo] sites are equidistant from the Zn(II) sites. We conclude that these sites are identical with the three central Zn(II) sites present in insulin hexamer crystals soaked in excess Zn(II) [Emdin, S. O., Dodson, G., Cutfield, J. M., & Cutfield, S. M. (1980) Diabetologia 19, 174-182] and suggest that these central sites are occupied by Ca(II) in vivo.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Alameda GK,Evelhoch JL,Sudmeier JL,Birge RRdoi
10.1021/bi00328a028subject
Has Abstractpub_date
1985-03-26 00:00:00pages
1757-62issue
7eissn
0006-2960issn
1520-4995journal_volume
24pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::The egg vitelline envelope of the marine invertebrate, Megathura crenulata, was lyzed either by sperm lysins A, B, C or by dithiothreitol. In each case the lysis mixture consisted of two major fractions, I and II, that could be separated by hydroxylapatite chromatography and had different electrophoretic mobilities on...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00651a003
更新日期:1976-03-23 00:00:00
abstract::Electron spin echo envelope modulation (ESEEM) spectroscopy has been applied to the determination of the number of water molecules coordinated to the metal in the binary complex of staphylococcal nuclease with Mn2+, to the ternary enzyme-Mn2+-3',5'-pdTp complex, and to ternary complexes of a number of mutant enzymes i...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00421a010
更新日期:1988-10-18 00:00:00
abstract::Phosphatidylcholine phospholipase C (EC 3.1.4.3) from Bacillus cereus has been assayed with substrates in the form of large unilamellar vesicles. Phosphatidylcholine, phosphatidylethanolamine (also a substrate for the enzyme), sphingomyelin, and cholesterol have been mixed in various proportions, in binary, ternary, a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi980615x
更新日期:1998-08-18 00:00:00
abstract::Aureochromes have recently been shown to act as blue-light-regulated transcription factors in the stramenopile alga Vaucheria frigida. They comprise a light-, oxygen-, or voltage-sensitive (LOV) domain as a sensory module with flavin mononucleotide (FMN) as a chromophore and a basic region leucine zipper (bZIP) domain...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi400197u
更新日期:2013-05-07 00:00:00
abstract::Nitrogenase catalyzes the reduction of N2 to NH3, supporting all biological nitrogen fixation. Electron donors to this enzyme are ferredoxin or flavodoxin (in vivo) and sodium dithionite (in vitro). Features of these electron donors put a limit on spectrophotometric studies and electrocatalytic applications of nitroge...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.9b00844
更新日期:2019-11-19 00:00:00
abstract::A detailed kinetic analysis of the catalytic trimer of aspartate transcarbamoylase containing the active site substitution H134A was performed to investigate the role of His 134 in the catalytic mechanism. Replacement of histidine by alanine resulted in decreases in the affinities for the two substrates, carbamoyl pho...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00143a031
更新日期:1992-07-21 00:00:00
abstract::Two novel heme-containing peroxidases, one able to incorporate halogens into aromatic substrates and the other able to remove them, have recently been isolated from marine sources and initially characterized by Chen et al. [(1991) J. Biol. Chem. 266, 23909-23915; (1996) J. Biol. Chem. 271, 4609-4612]. The haloperoxida...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9621371
更新日期:1997-02-25 00:00:00
abstract::Tyr71 is an invariant residue in all known sequences of tyrosine phenol-lyase (TPL). The substitution of Tyr71 in TPL by phenylalanine results in a mutant Y71F TPL with no detectable activity (greater than 3 x 10(5)-fold reduction) for beta-elimination of L-tyrosine. Y71F TPL can react with S-alkylcysteines, but these...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00038a023
更新日期:1995-09-26 00:00:00
abstract::Tyrosinase is a copper monooxygenase containing a coupled dinuclear copper active site (type-3 copper), which catalyzes oxygenation of phenols (phenolase activity) as well as dehydrogenation of catechols (catecholase activity) using O(2) as the oxidant. In this study, catalase activity (conversion of H(2)O(2) to (1/2)...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi048908f
更新日期:2004-09-14 00:00:00
abstract::Actin interaction with L-plastin, a plastin/fimbrins isoform of the alpha-actinin family of molecules, is poorly characterized, from the biochemical point of view. Besides, molecular modeling of the T-isoform has recently provided a complete model of interaction with filamentous actin [Volkmann, N., DeRosier, D., Mats...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi030151p
更新日期:2004-03-09 00:00:00
abstract::Picosecond transient absorption difference spectroscopic experiments were performed on membranes of the antenna/reaction center complex of Heliobacillus mobilis to study the electron transfer processes. Particular emphasis was placed on the blue spectral region, where the difference spectra of iron-sulfur centers and ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00039a036
更新日期:1995-10-03 00:00:00
abstract::The VWF A1 domain seems to possess two heparin binding regions (residues 565-587 and 633-648) displaying positively charged amino acids, but the overall polyanion-A1 domain interaction scheme remains essentially elusive. To probe this molecular reaction as well as the role of electrostatic forces in VWF-heparin intera...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi020044f
更新日期:2002-05-28 00:00:00
abstract::Proteins from the enhanced intracellular survival (Eis) family are versatile acetyltransferases that acetylate amines at multiple positions of several aminoglycosides (AGs). Their upregulation confers drug resistance. Homologues of Eis are present in diverse bacteria, including many pathogens. Eis from Mycobacterium t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b00244
更新日期:2015-05-26 00:00:00
abstract::Nhp6A is an abundant non-histone chromatin-associated protein in Saccharomyces cerevisiae that contains a minor groove DNA binding motif called the HMG box. In this report, we show that Nhp6Ap binds to cisplatin intrastrand cross-links on duplex DNA with a 40-fold greater affinity than to unmodified DNA with the same ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi012077l
更新日期:2002-04-30 00:00:00
abstract::Protease inhibition by metastable serine protease inhibitors (serpins) is mediated by one of the largest functional intradomain conformational changes known in biology. In this extensive structural rearrangement, protease-serpin complex formation triggers cleavage of the serpin reactive center loop (RCL), its subseque...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00839
更新日期:2017-01-31 00:00:00
abstract::The effects of nonylphenol and 3,5-dibutyl-4-hydroxytoluene (BHT) on the activity of the (Ca2(+)-Mg2+)-ATPase of skeletal muscle sarcoplasmic reticulum have been studied. At high concentrations, both inhibit the ATPase activity of the ATPase either in native lipid or in bilayers of dioleoylphosphatidylcholine but, at ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00464a028
更新日期:1990-03-27 00:00:00
abstract::The agouti-related protein gene (Agrp) plays an important role in body weight regulation. The mature human protein is a single polypeptide chain of 112 amino acid residues, consisting of an N-terminal acidic region and a unique C-terminal cysteine-rich domain. The disulfide structure of recombinant human AGRP was dete...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi981082v
更新日期:1998-09-01 00:00:00
abstract::Periodate-oxidized tRNA(Phe) (tRNA(oxPhe)) behaves as a specific affinity label of tetrameric Escherichia coli phenylalanyl-tRNA synthetase (PheRS). Reaction of the alpha 2 beta 2 enzyme with tRNA(oxPhe) results in the loss of tRNAPhe aminoacylation activity with covalent attachment of 2 mol of tRNA dialdehyde/mol of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00391a033
更新日期:1987-08-25 00:00:00
abstract::Cytochrome P450 reductase (CPR) is a tethered membrane protein which transfers electrons from NADPH to microsomal P450s. We show that the lipid bilayer has a role in defining the redox potential of the CPR flavin domains. In order to quantitate the electrochemical behavior of this central redox protein, full-length CP...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9011435
更新日期:2009-12-29 00:00:00
abstract::Ultraviolet resonance Raman (RR) spectra, with 200- and 218-nm excitation from a H2-shifted quadrupled Nd:YAG laser, are reported for insulin and alpha-lactalbumin in dilute aqueous solution, at pH values known to produce differences in the exposure of the aromatic residues to solvent. At 200 nm, the spectra are domin...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00329a009
更新日期:1985-04-09 00:00:00
abstract::Electron paramagnetic resonance studies have indicated that nitrosodisulfonate binds to pig heart citrate synthase. Titration of the enzyme with nitrosodisulfonate revealed several binding sites for the probe per subunit with one site (KD approximately 0.1 mM) having a greater affinity than the others. The substrate, ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00584a028
更新日期:1979-08-21 00:00:00
abstract::The synthesis is described of four new cleavable, photosensitive, heterobifunctional cross-linking reagents for use in examining membrane organization: 4'-azidoazobenzene-4oxysuccinimide ester (1), N-[4-(p-azidophenylazo)benzoyl]-3-aminopropyl-N'-oxysuccinimide ester (2), N-[4-(p-azidophenylazo)benzoyl]-6-aminohexyl-N...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00560a007
更新日期:1980-09-16 00:00:00
abstract::Two site-directed mutant enzymes of the class A beta-lactamase from Staphylococcus aureus PC1 were produced with the goal of blocking the site that in the native enzyme is occupied by the proposed hydrolytic water molecule. The crystal structures of these two mutant enzymes, N170Q and N170M, have been determined and r...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi962242a
更新日期:1996-12-24 00:00:00
abstract::Factor X (FX) is one of the major players in the blood coagulation cascade. Upon activation to FXa, it converts prothrombin to thrombin, which in turn converts fibrinogen into fibrin (blood clots). FXa deficiency causes hemostasis defects, such as intracranial bleeding, hemathrosis, and gastrointestinal blood loss. He...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi500770p
更新日期:2014-11-11 00:00:00
abstract::The insulin-linked polymorphic region (ILPR) is a regulatory sequence in the promoter region upstream of the human insulin gene and is widely recognized as a locus of type 1 diabetes susceptibility. Polymorphism of the ILPR sequence can affect expression of both insulin and the adjacent insulin-like growth factor 2 (I...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi900958x
更新日期:2009-09-01 00:00:00
abstract::The processes of liposome solubilization and reconstitution were studied by using n-dodecyl octaethylene glycol monoether (C12E8). The solubilization of large unilamellar liposomes prepared by reverse-phase evaporation was systematically investigated by turbidity, 31P nuclear magnetic resonance, and centrifugation exp...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00492a022
更新日期:1990-10-09 00:00:00
abstract::The differential scanning calorimetry trace of F1ATPase, prepared from beef heart submitochondrial particles, has a single sharp endothermic transition at 80.5 +/- 1.0 degrees C and a half-height peak width of 2.0 +/- 0.2 degrees. The transition enthalpy is 19 +/- 2 cal/g of protein. Submitochondrial particles (SMP) h...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00328a022
更新日期:1985-03-26 00:00:00
abstract::The molecular structure of ammonium deoxycytidylyl-(3'-5')-deoxyguanosine, crystallized from aqueous acetone near pH 4, was determined for X-ray diffraction data. The crystals were tetragonal, space group P43212 with a = b = 11.078 (1) A and c = 45.826 (4) A. The structure was solved by tangent expansion of phases bas...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00277a014
更新日期:1983-04-12 00:00:00
abstract::Phospholipase A(2) (PLA(2)) (E. C. 3.1.1.4) is a common enzyme in the two-way cascade mechanism leading to the production of proinflammatory compounds known as eicosanoids. The binding of phospholipase A(2) to the membrane surface and hydrolysis of phospholipids are thought to involve the formation of a hydrophobic ch...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi035076x
更新日期:2003-10-14 00:00:00
abstract::Glu58 is known to participate in phosphodiester transesterification catalyzed by the enzyme RNase T1. For Glu58 RNase T1, an altered mechanism has been proposed in which His40 replaces Glu58 as the base catalyst [Steyaert, J., Hallenga, K., Wyns, L., & Stanssens, P. (1990) Biochemistry 29, 9064-9072]. Glu58Ala Rnase T...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00173a006
更新日期:1994-02-22 00:00:00