当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Phospholipase C hydrolysis of phospholipids in bilayers of mixed lipid compositions.

Phospholipase C hydrolysis of phospholipids in bilayers of mixed lipid compositions.

Abstract：

:Phosphatidylcholine phospholipase C (EC 3.1.4.3) from Bacillus cereus has been assayed with substrates in the form of large unilamellar vesicles. Phosphatidylcholine, phosphatidylethanolamine (also a substrate for the enzyme), sphingomyelin, and cholesterol have been mixed in various proportions, in binary, ternary, and quaternary mixtures. A lag period, followed by a burst of enzyme activity, has been found in all cases. The activity burst was always accompanied by an increase in turbidity of the vesicle suspension. Varying lipid compositions while keeping constant all the other parameters leads to a range of lag times extending over 2 orders of magnitude (from 0.13 to 38.0 min), and a similar variability is found in maximal enzyme rates (from 0.40 to 55.9 min-1). Meanwhile, the proportion of substrate that is hydrolyzed during the lag period remains relatively constant at 0.10% moles of total lipid, in agreement with the idea that enzyme activation is linked to vesicle aggregation through diacylglycerol-rich patches. Phosphatidylethanolamine and cholesterol enhance the enzyme activity in a dose-dependent way: they reduce the lag times and increase the maximal rates. The opposite is true of sphingomyelin. These lipids exert each its own peculiar effect, positive or negative, either alone or in combination, so that the susceptibility of a given mixture to the enzyme activity can be to some extent predicted from its composition. Phospholipase C activity is not directly influenced by the formation of nonlamellar structures. However, the presence of lipids with a tendency to form nonlamellar phases, such as phosphatidylethanolamine or cholesterol, stimulates the enzyme even under conditions at which purely lamellar phases exist. Conversely sphingomyelin, a well-known stabilizer of the lamellar phase, inhibits the enzyme. Thus phospholipase C appears to be regulated by the overall geometry and composition of the bilayer.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Ruiz-Argüello MB,Goñi FM,Alonso A

doi

10.1021/bi980615x

subject

Has Abstract

pub_date

1998-08-18 00:00:00

pages

11621-8

issue

33

eissn

0006-2960

issn

1520-4995

pii

bi980615x

journal_volume

37

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Differential effects on energy transduction processes by fluorescamine derivatives in rat liver mitochondria.

    abstract：:Intact rat liver mitochondria were treated with compounds derived from the reaction of fluorescamine with various types of primary amines, including the mycosamine-containing antibiotics amphotericin B and nystatin. The effect of varying amounts of these compounds on ATPase-linked inorganic phosphate (Pi) formation on...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00550a031

    authors： Ramirez F,Shiuan D,Tu SI,Marecek JF

    更新日期：1980-04-29 00:00:00

  • A bacterially expressed mineralocorticoid receptor is associated in vitro with the 90-kilodalton heat shock protein and shows typical hormone- and DNA-binding characteristics.

    abstract：:A recombinant system was developed for generation of steroid-receptor complexes in vitro. The DNA- and steroid-binding domains of the rat mineralocorticoid receptor were expressed in Escherichia coli as a fusion protein with glutathione S-transferase. The identity of the expressed recombinant protein was confirmed by ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00084a028

    authors： Caamaño CA,Morano MI,Patel PD,Watson SJ,Akil H

    更新日期：1993-08-24 00:00:00

  • Role of the C-terminus of the high-conductance calcium-activated potassium channel in channel structure and function.

    abstract：:The role of ion channels in cell physiology is regulated by processes occurring after protein biosynthesis, which are critical for both channel function and targeting of channels to appropriate cell compartments. Here we apply biochemical and electrophysiological methods to investigate the role of the high-conductance...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi050527u

    authors： Schmalhofer WA,Sanchez M,Dai G,Dewan A,Secades L,Hanner M,Knaus HG,McManus OB,Kohler M,Kaczorowski GJ,Garcia ML

    更新日期：2005-08-02 00:00:00

  • Structure and stability of apolipoprotein a-I in solution and in discoidal high-density lipoprotein probed by double charge ablation and deletion mutation.

    abstract：:To identify residues and segments in the central region of apolipoprotein A-I (apoA-I) that are important for the protein structure and stability, we studied the effects of four double charge ablations, D102A/D103A, E110A/E111A, R116V/K118A, and R160V/H162A, and two deletion mutations, Delta(61-78) and Delta(121-142),...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi051669r

    authors： Gorshkova IN,Liu T,Kan HY,Chroni A,Zannis VI,Atkinson D

    更新日期：2006-01-31 00:00:00

  • Solid-State Nuclear Magnetic Resonance Structural Study of the Retinal-Binding Pocket in Sodium Ion Pump Rhodopsin.

    abstract：:The recently identified Krokinobacter rhodopsin 2 (KR2) functions as a light-driven sodium ion pump. The structure of the retinal-binding pocket of KR2 offers important insights into the mechanisms of KR2, which has motif of Asn112, Asp116, and Gln123 (NDQ) that is common among sodium ion pump rhodopsins but is unique...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.6b00999

    authors： Shigeta A,Ito S,Inoue K,Okitsu T,Wada A,Kandori H,Kawamura I

    更新日期：2017-01-31 00:00:00

  • Analysis of the kinetics of ovine follitropin agonist-antagonist interactions with pig ovarian membranes.

    abstract：:The binding of 125I-labeled ovine follitropin (oFSH) and 125I-labeled deglycosylated ovine follitropin (DG-oFSH) to porcine granulosa cell membranes was studied at equilibrium and nonequilibrium binding conditions and statistically analyzed. Saturation and competition binding experiments revealed homogeneity in the po...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00386a059

    authors： Sebok K,De Lean A,Sairam MR

    更新日期：1987-06-16 00:00:00

  • NMR solution structure of the C-terminal fragment 255-316 of thermolysin: a dimer formed by subunits having the native structure.

    abstract：:The solution structure of the C-terminal fragment 255-316 of thermolysin has been determined by two-dimensional proton NMR methods. For this disulfide-free fragment there was a previous proposal according to which it would fold into a stable helical structure forming a dimer at concentrations above 0.06 mM. A complete...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00253a023

    authors： Rico M,Jiménez MA,González C,De Filippis V,Fontana A

    更新日期：1994-12-13 00:00:00

  • Heat shock protein 80 of Neurospora crassa, a cytosolic molecular chaperone of the eukaryotic stress 90 family, interacts directly with heat shock protein 70.

    abstract：:The subunit structure of Hsp80, the most abundant heat-shock protein of Neurospora crassa, was examined by chemical cross-linking of the purified protein in vitro. Resolution of glutaraldehyde-treated Hsp80 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis SDS-PAGE suggests that the native state of this pro...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi963030g

    authors： Freitag DG,Ouimet PM,Girvitz TL,Kapoor M

    更新日期：1997-08-19 00:00:00

  • Conformational study of the potent peptide hormone antagonist [1-penicillamine,2-leucine]oxytocin in aqueous solution.

    abstract：:[1-Penicillamine,2-leucine]oxytocin is a conformationally restricted analogue of oxytoxin in which the half-cystine-1 and tyrosine-2 residues of the native hormone are replaced by half-penicillamine (beta, beta-dimethyl-half-cystine) and leucine, respectively. This analogue is a surprisingly potent oxytocin antagonist...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00513a018

    authors： Mosberg HI,Hruby VJ,Meraldi JP

    更新日期：1981-05-12 00:00:00

  • Pre-steady-state kinetics reveal a slow isomerization of the enzyme-NAD complex in the NAD-malic enzyme reaction.

    abstract：:Stopped-flow experiments obtained in the pre-steady-state time scale of the NAD-malic enzyme reaction exhibit a lag prior to the attainment of steady state. Previous results from isotope effect studies in which the deuterium isotope effect on Vmax decreases to a value of 1 at low pH have been interpreted as suggesting...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00059a007

    authors： Rajapaksa R,Abu-Soud H,Raushel FM,Harris BG,Cook PF

    更新日期：1993-03-02 00:00:00

  • 1H NMR observation of redox potential in liver.

    abstract：:1H NMR spectral editing techniques can select the distinct signals of lactate, pyruvate, beta-hydroxybutyrate, and acetoacetate and provide a unique way to monitor the biochemical processes in vivo. These metabolite levels reflect the near-equilibrium dehydrogenase activity and therefore the cellular redox state. The ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00160a029

    authors： Chung Y,Jue T

    更新日期：1992-11-17 00:00:00

  • Probing the bimolecular interactions of parathyroid hormone with the human parathyroid hormone/parathyroid hormone-related protein receptor. 1. Design, synthesis and characterization of photoreactive benzophenone-containing analogs of parathyroid hormone.

    abstract：:Parathyroid hormone (PTH) regulates calcium and phosphate metabolism through a G-protein-coupled receptor which is shared with PTH-related protein (PTHrP). Therefore, structure-activity studies of PTH and PTHrP with their common receptor provide an unusual opportunity to examine the structural elements in the two horm...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00033a029

    authors： Nakamoto C,Behar V,Chin KR,Adams AE,Suva LJ,Rosenblatt M,Chorev M

    更新日期：1995-08-22 00:00:00

  • Structure of a Zinc Porphyrin-Substituted Bacterioferritin and Photophysical Properties of Iron Reduction.

    abstract：:The iron storage protein bacterioferritin (Bfr) binds up to 12 hemes b at specific sites in its protein shell. The heme b can be substituted with the photosensitizer Zn(II)-protoporphyrin IX (ZnPP), and photosensitized reductive iron release from the ferric oxyhydroxide {[FeO(OH)] n ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.9b01103

    authors： Benavides BS,Valandro S,Cioloboc D,Taylor AB,Schanze KS,Kurtz DM Jr

    更新日期：2020-04-28 00:00:00

  • Sequence-Dependent Promoter Escape Efficiency Is Strongly Influenced by Bias for the Pretranslocated State during Initial Transcription.

    abstract：:Abortive transcription initiation can be rate-limiting for promoter escape and therefore represents a barrier to productive gene expression. The mechanism for abortive initiation is unknown, but the amount of abortive transcript is known to vary with the composition of the initial transcribed sequence (ITS). Here, we ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b00272

    authors： Skancke J,Bar N,Kuiper M,Hsu LM

    更新日期：2015-07-21 00:00:00

  • Protein Synthesis with Ribosomes Selected for the Incorporation of β-Amino Acids.

    abstract：:In an earlier study, β³-puromycin was used for the selection of modified ribosomes, which were utilized for the incorporation of five different β-amino acids into Escherichia coli dihydrofolate reductase (DHFR). The selected ribosomes were able to incorporate structurally disparate β-amino acids into DHFR, in spite of...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b00389

    authors： Maini R,Chowdhury SR,Dedkova LM,Roy B,Daskalova SM,Paul R,Chen S,Hecht SM

    更新日期：2015-06-16 00:00:00

  • Specific photoaffinity labeling of Tyr-49 on the light chain in the steroid-combining site of a mouse monoclonal anti-estradiol antibody using two epimeric 6alpha- and 6beta-(5-azido-2-nitrobenzoyl)amidoestradiol photoreagents.

    abstract：:A mouse monoclonal anti-7-(O-carboxymethyl)oximinoestradiol antibody was photoaffinity labeled with two cross-reactive 6alpha- and 6beta-(5-azido-2-nitrobenzoyl)amido[17alpha-3H]estradiol photoreagents (6alpha- and 6beta-ANBA-[17alpha-3H]estradiol). Covalently bound radioactivity was found exclusively on the light cha...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi962645a

    authors： Rousselot P,Mappus E,Blachère T,de Ravel MR,Grenot C,Tonnelle C,Cuilleron CY

    更新日期：1997-06-24 00:00:00

  • A conserved amphipathic ligand binding region influences k-path-dependent activity of cytochrome C oxidase.

    abstract：:A conserved, crystallographically defined bile acid binding site was originally identified in the membrane domain of mammalian and bacterial cytochrome c oxidase (CcO). Current studies show other amphipathic molecules including detergents, fatty acids, steroids, and porphyrins bind to this site and affect the already ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi3014505

    authors： Hiser C,Buhrow L,Liu J,Kuhn L,Ferguson-Miller S

    更新日期：2013-02-26 00:00:00

  • A ParE-ParC fusion protein is a functional topoisomerase.

    abstract：:Type II topoisomerases are responsible for DNA unlinking during DNA replication and chromosome segregation. Although eukaryotic enzymes are homodimers and prokaryotic enzymes are heterotetramers, both prokaryotic and eukaryotic type II topoisomerases belong to a single protein family. The amino- and carboxyl-terminal ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0155201

    authors： Lavasani LS,Hiasa H

    更新日期：2001-07-24 00:00:00

  • Serine protease of hepatitis C virus expressed in insect cells as the NS3/4A complex.

    abstract：:Hepatitis C virus (HCV) protease NS3 and its protein activator NS4A participate in the processing of the viral polyprotein into its constituent nonstructural proteins. The NS3/4A complex is thus an attractive target for antiviral therapy against HCV. We expressed the full-length NS3 and NS4A in insect cells as a solub...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi972010r

    authors： Sali DL,Ingram R,Wendel M,Gupta D,McNemar C,Tsarbopoulos A,Chen JW,Hong Z,Chase R,Risano C,Zhang R,Yao N,Kwong AD,Ramanathan L,Le HV,Weber PC

    更新日期：1998-03-10 00:00:00

  • Mode of interaction of aminooxy compounds with sheep liver serine hydroxymethyltransferase.

    abstract：:The interaction of aminooxy compounds such as aminooxyacetate (AAA), L-canaline, and hydroxylamine with sheep liver serine hydroxymethyltransferase (EC 2.1.2.1) was studied by absorption spectra and stopped-flow spectrophotometry and compared with the unique feature of interaction of O-amino-D-serine (OADS) with the e...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00451a010

    authors： Baskaran N,Prakash V,Savithri HS,Radhakrishnan AN,Appaji Rao N

    更新日期：1989-12-12 00:00:00

  • Insights into the stability of native and partially folded states of ubiquitin: effects of cosolvents and denaturants on the thermodynamics of protein folding.

    abstract：:The thermodynamics of the native<-->A state and native<-->unfolded transitions for ubiquitin have been characterized in detail using the denaturants methanol and guanidinium chloride (Gdn.HCl) both separately and in combination. Gdn.HCl destabilizes the partially folded alcohol-induced A state such that the effects of...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi010767j

    authors： Jourdan M,Searle MS

    更新日期：2001-08-28 00:00:00

  • Characterization of heme-regulated eIF2alpha kinase: roles of the N-terminal domain in the oligomeric state, heme binding, catalysis, and inhibition.

    abstract：:Heme-regulated eIF2alpha kinase [heme-regulated inhibitor (HRI)] plays a critical role in the regulation of protein synthesis by heme iron. The kinase active site is located in the C-terminal domain, whereas the N-terminal domain is suggested to regulate catalysis in response to heme binding. Here, we found that the r...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi060556k

    authors： Miksanova M,Igarashi J,Minami M,Sagami I,Yamauchi S,Kurokawa H,Shimizu T

    更新日期：2006-08-15 00:00:00

  • Investigation of the origin of the "S3" EPR signal from the oxygen-evolving complex of photosystem 2: the role of tyrosine Z.

    abstract：:The origin of the "S3" EPR signal from calcium-depleted photosystem 2 samples has been investigated. This signal is observed after freezing samples under illumination and has been assigned to an interaction between the manganese cluster and an oxidized histidine radical [Boussac et al. (1990) Nature 347; 303-306]. In ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00134a005

    authors： Hallahan BJ,Nugent JH,Warden JT,Evans MC

    更新日期：1992-05-19 00:00:00

  • Delta-elimination by T4 endonuclease V at a thymine dimer site requires a secondary binding event and amino acid Glu-23.

    abstract：:Endonuclease V from bacteriophage T4 is a well characterized enzyme that initiates the repair of ultraviolet light induced pyrimidine dimers. Scission of the phosphodiester backbone between the pyrimidines within a dimer, or 3' to an abasic (AP) site, occurs by a beta-elimination mechanism. In addition, high concentra...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00027a031

    authors： Latham KA,Lloyd RS

    更新日期：1995-07-11 00:00:00

  • Thermodynamic analysis of unfolding and dissociation in lactose repressor protein.

    abstract：:Lactose repressor protein, regulator of lac enzyme expression in Escherichia coli, maintains its structure and function at extremely low protein concentrations (<10(-)12 M). To examine the unfolding and dissociation of this tetrameric protein, structural transitions in the presence of varying concentrations of urea we...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9900727

    authors： Barry JK,Matthews KS

    更新日期：1999-05-18 00:00:00

  • Mutant IDH1 enhances the production of 2-hydroxyglutarate due to its kinetic mechanism.

    abstract：:The human, cytosolic enzyme isocitrate dehydrogenase 1 (IDH1) reversibly converts isocitrate to α-ketoglutarate (αKG). Cancer-associated somatic mutations in IDH1 result in a loss of this normal function but a gain in a new or neomorphic ability to convert αKG to the oncometabolite 2-hydroxyglutarate (2HG). To improve...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi400514k

    authors： Rendina AR,Pietrak B,Smallwood A,Zhao H,Qi H,Quinn C,Adams ND,Concha N,Duraiswami C,Thrall SH,Sweitzer S,Schwartz B

    更新日期：2013-07-02 00:00:00

  • Transthyretin mutation Leu-55-Pro significantly alters tetramer stability and increases amyloidogenicity.

    abstract：:A recently reported variant of human transthyretin (TTR), Leu-55-Pro, implicated as the causative agent in early-onset familial amyloid polyneuropathy was expressed and characterized, and its denaturation pathway and amyloidogenicity were compared to those of wild-type transthyretin. The overlap-extension polymerase c...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00096a024

    authors： McCutchen SL,Colon W,Kelly JW

    更新日期：1993-11-16 00:00:00

  • CHOBIMALT: a cholesterol-based detergent.

    abstract：:Cholesterol and its hemisuccinate and sulfate derivatives are widely used in studies of purified membrane proteins but are difficult to solubilize in aqueous solution, even in the presence of detergent micelles. Other cholesterol derivatives do not form conventional micelles and lead to viscous solutions. To address t...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi101334j

    authors： Howell SC,Mittal R,Huang L,Travis B,Breyer RM,Sanders CR

    更新日期：2010-11-09 00:00:00

  • The vitelline envelope of eggs from the giant keyhole limpet Megathura crenulata. II. Products formed by lysis with sperm enzymes and dithiothreitol.

    abstract：:The egg vitelline envelope of the marine invertebrate, Megathura crenulata, was lyzed either by sperm lysins A, B, C or by dithiothreitol. In each case the lysis mixture consisted of two major fractions, I and II, that could be separated by hydroxylapatite chromatography and had different electrophoretic mobilities on...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00651a003

    authors： Heller E,Raftery MA

    更新日期：1976-03-23 00:00:00

  • Maltosyl isothiocyanate: an affinity label for the glucose transporter of the human erythrocyte membrane. 1. Inhibition of glucose transport.

    abstract：:Maltosyl isothiocyanate (MITC) has been synthesized from maltose with an overall yield of 88%. It has been found to be a potent irreversible inhibitor of zero trans influx of glucose with human erythrocytes. Kinetic analysis of glucose transport after treatment of erythrocytes with MITC revealed that VT was diminished...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00547a025

    authors： Mullins RE,Langdon RG

    更新日期：1980-03-18 00:00:00