Maltosyl isothiocyanate: an affinity label for the glucose transporter of the human erythrocyte membrane. 1. Inhibition of glucose transport.

Abstract:

:Maltosyl isothiocyanate (MITC) has been synthesized from maltose with an overall yield of 88%. It has been found to be a potent irreversible inhibitor of zero trans influx of glucose with human erythrocytes. Kinetic analysis of glucose transport after treatment of erythrocytes with MITC revealed that VT was diminished while KT was unchanged. Transportable sugars and competitive inhibitors of monosaccharide transport protected against MITC inhibition, while carbohydrates which do not interact with the transporter gave no protection. Covalent inhibitors of anion transport were without effect on glucose transport. MITC fulfilled the kinetic requirements for an affinity label of the glucose transporter of human erythrocytes [Groman, E. V., Schultz, R. M., & Engel, L. L. (1977) Methods Enzymol. 46, 54].

journal_name

Biochemistry

journal_title

Biochemistry

authors

Mullins RE,Langdon RG

doi

10.1021/bi00547a025

subject

Has Abstract

pub_date

1980-03-18 00:00:00

pages

1199-205

issue

6

eissn

0006-2960

issn

1520-4995

journal_volume

19

pub_type

杂志文章
  • Ligand-induced variations in the reactivity of thio groups of the alpha-subunit of the acetylcholine receptor from Torpedo californica.

    abstract::We have studied alkylation of the acetylcholine receptor by N-[3H]ethylmaleimide ([3H]NEM) under various conditions. The radiolabeled preparations were submitted to sodium dodecyl sulfate-polyacrylamide gel electrophoresis to separate the receptor complex into subunits, and the incorporation of 3H into each type of ch...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00306a001

    authors: de Souza Otero A,Hamilton SL

    更新日期:1984-05-22 00:00:00

  • Crystal structure of eta-crystallin: adaptation of a class 1 aldehyde dehydrogenase for a new role in the eye lens.

    abstract::Eta-crystallin is a retinal dehydrogenase that has acquired a role as a structural protein in the eye lens of elephant shrews, members of an ancient order of mammals. While it retains some activity toward retinal, which is oxidized to retinoic acid, the protein has acquired a number of specific sequence changes that h...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi027367w

    authors: Bateman OA,Purkiss AG,van Montfort R,Slingsby C,Graham C,Wistow G

    更新日期:2003-04-22 00:00:00

  • Real-time measurement of multiple intramolecular distances during protein folding reactions: a multisite stopped-flow fluorescence energy-transfer study of yeast phosphoglycerate kinase.

    abstract::Understanding the set of rules which dictate how the primary amino acid sequence determines tertiary structure is an unsolved problem in biophysics. If it were possible to simultaneously measure all of the intramolecular distances in a protein (in real time) during a folding reaction, the "second" genetic code problem...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi970789z

    authors: Lillo MP,Szpikowska BK,Mas MT,Sutin JD,Beechem JM

    更新日期:1997-09-16 00:00:00

  • Structural Basis for Excision of 5-Formylcytosine by Thymine DNA Glycosylase.

    abstract::Thymine DNA glycosylase (TDG) is a base excision repair enzyme with key functions in epigenetic regulation. Performing a critical step in a pathway for active DNA demethylation, TDG removes 5-formylcytosine and 5-carboxylcytosine, oxidized derivatives of 5-methylcytosine that are generated by TET (ten-eleven transloca...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/acs.biochem.6b00982

    authors: Pidugu LS,Flowers JW,Coey CT,Pozharski E,Greenberg MM,Drohat AC

    更新日期:2016-11-15 00:00:00

  • Studies on the hydrolytic properties of (serine) carboxypeptidase Y.

    abstract::The activity of serine carboxypeptidases is dependent on a catalytic triad, an oxyanion hole, and a binding site equivalent to those found in the serine endopeptidases. The action of carboxypeptidase Y on substrates containing amino acids, alcohols, and amines as leaving groups is described. It is demonstrated that th...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi952758e

    authors: Stennicke HR,Mortensen UH,Breddam K

    更新日期:1996-06-04 00:00:00

  • GrpE accelerates nucleotide exchange of the molecular chaperone DnaK with an associative displacement mechanism.

    abstract::The ATP hydrolysis and protein-binding and release cycle of the molecular chaperone DnaK is regulated by the accessory proteins GrpE and DnaJ. Here we describe a study of the formation of complexes between the molecular chaperone DnaK, its nucleotide exchange factor GrpE, and the fluorescent ADP analog N8-[4-[(N'-meth...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi962835l

    authors: Packschies L,Theyssen H,Buchberger A,Bukau B,Goody RS,Reinstein J

    更新日期:1997-03-25 00:00:00

  • Quantification of quaternary structure stability in aggregation-prone proteins under physiological conditions: the transthyretin case.

    abstract::The quaternary structure stability of proteins is typically studied under conditions that accelerate their aggregation/unfolding processes on convenient laboratory time scales. Such conditions include high temperature or pressure, chaotrope-mediated unfolding, or low or high pH. These approaches have the limitation of...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi500739q

    authors: Robinson LZ,Reixach N

    更新日期:2014-10-21 00:00:00

  • Characterization of the fluorophore 4-heptadecyl-7-hydroxycoumarin: a probe for the head-group region of lipid bilayers and biological membranes.

    abstract::The fluorophore 4-heptadecyl-7-hydroxycoumarin was used as a probe to study the properties of phospholipid bilayers at the lipid-water interface. To this end, the steady-state fluorescence anisotropy, the differential polarized phase fluorometry, and the emission lifetime of the fluorophore were measured in isotropic ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00324a005

    authors: Pal R,Petri WA Jr,Ben-Yashar V,Wagner RR,Barenholz Y

    更新日期:1985-01-29 00:00:00

  • An Open Library of Human Kinase Domain Constructs for Automated Bacterial Expression.

    abstract::Kinases play a critical role in cellular signaling and are dysregulated in a number of diseases, such as cancer, diabetes, and neurodegeneration. Therapeutics targeting kinases currently account for roughly 50% of cancer drug discovery efforts. The ability to explore human kinase biochemistry and biophysics in the lab...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/acs.biochem.7b01081

    authors: Albanese SK,Parton DL,Işık M,Rodríguez-Laureano L,Hanson SM,Behr JM,Gradia S,Jeans C,Levinson NM,Seeliger MA,Chodera JD

    更新日期:2018-08-07 00:00:00

  • Transthyretin aggregation under partially denaturing conditions is a downhill polymerization.

    abstract::The deposition of fibrils and amorphous aggregates of transthyretin (TTR) in patient tissues is a hallmark of TTR amyloid disease, but the molecular details of amyloidogenesis are poorly understood. Tetramer dissociation is typically rate-limiting for TTR amyloid fibril formation, so we have used a monomeric variant o...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi049621l

    authors: Hurshman AR,White JT,Powers ET,Kelly JW

    更新日期:2004-06-15 00:00:00

  • Radical redesign of a tandem array of four R67 dihydrofolate reductase genes yields a functional, folded protein possessing 45 substitutions.

    abstract::R67 dihydrofolate reductase (DHFR) is a plasmid-encoded, type II enzyme. Four monomers (78 amino acids long) assemble into a homotetramer possessing 222 symmetry. In previous studies, a tandem array of four R67 DHFR gene copies was fused in frame to generate a functional monomer named Quad1. This protein possessed the...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi1005943

    authors: Feng J,Grubbs J,Dave A,Goswami S,Horner CG,Howell EE

    更新日期:2010-08-31 00:00:00

  • Characterization of a fluorescent substance P analog.

    abstract::We describe the development and characterization of substance P labeled at Lys3 with fluorescein ([fluorescein Lys3]SP) as a fluorescent probe for the neurokinin 1 (NK1) receptor. [fluorescein Lys3]SP is an agonist at the human NK1 receptor, with an affinity for both the high-affinity and low-affinity binding states o...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00248a017

    authors: Tota MR,Daniel S,Sirotina A,Mazina KE,Fong TM,Longmore J,Strader CD

    更新日期:1994-11-08 00:00:00

  • Photochemical cross-linking of psoralen-derivatized oligonucleoside methylphosphonates to rabbit globin messenger RNA.

    abstract::Antisense oligodeoxyribonucleoside methylphosphonates targeted against various regions of mRNA or precursor mRNA are selective inhibitors of mRNA expression both in cell-free systems and in cells in culture. The efficiency with which methylphosphonate oligomers interact with mRNA, and thus inhibit translation, can be ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00426a008

    authors: Kean JM,Murakami A,Blake KR,Cushman CD,Miller PS

    更新日期:1988-12-27 00:00:00

  • Chemical mechanism of a cysteine protease, cathepsin C, as revealed by integration of both steady-state and pre-steady-state solvent kinetic isotope effects.

    abstract::Cathepsin C, or dipeptidyl peptidase I, is a lysosomal cysteine protease of the papain family that catalyzes the sequential removal of dipeptides from the free N-termini of proteins and peptides. Using the dipeptide substrate Ser-Tyr-AMC, cathepsin C was characterized in both steady-state and pre-steady-state kinetic ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi8007627

    authors: Schneck JL,Villa JP,McDevitt P,McQueney MS,Thrall SH,Meek TD

    更新日期:2008-08-19 00:00:00

  • Partitioning of fluorescent phospholipid probes between different bilayer environments. Estimation of the free energy of interlipid hydrogen bonding.

    abstract::Fluorescence spectroscopy has been used to monitor the partitioning of a series of exchangeable neutral phospholipid probes, labeled with carbazole, indolyl or diphenylhexatrienyl moieties, between large unilamellar vesicles containing 1-palmitoyl-2-oleoylphosphatidylcholine (POPC), 1,2-dioleoyloxy-3-(trimethylammonio...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00244a018

    authors: Shin TB,Leventis R,Silvius JR

    更新日期:1991-07-30 00:00:00

  • Involvement of Phenylalanine 297 in the Construction of the Substrate Pocket of Human Aminopeptidase B.

    abstract::Aminopeptidase B (APB, EC 3.4.11.6) preferentially hydrolyzes the N-terminal basic amino acids of synthetic and peptide substrates and requires a physiological concentration of NaCl for optimal activity. In this study, we used site-directed mutagenesis and molecular modeling to search for an amino acid residue that is...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/acs.biochem.5b00964

    authors: Ohnishi A,Watanabe J,Ogawa Y,Goto Y,Hattori A,Tsujimoto M

    更新日期:2015-10-06 00:00:00

  • A conservative isoleucine to leucine mutation causes major rearrangements and cold sensitivity in KlenTaq1 DNA polymerase.

    abstract::Assembly of polymerase chain reactions at room temperature can sometimes lead to low yields or unintentional products due to mispriming. Mutation of isoleucine 707 to leucine in DNA polymerase I from Thermus aquaticus substantially decreases its activity at room temperature without compromising its ability to amplify ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi501198f

    authors: Wu EY,Walsh AR,Materne EC,Hiltner EP,Zielinski B,Miller BR 3rd,Mawby L,Modeste E,Parish CA,Barnes WM,Kermekchiev MB

    更新日期:2015-01-27 00:00:00

  • pH-dependent charge equilibria between tyrosine-D and the S states in photosystem II. Estimation of relative midpoint redox potentials.

    abstract::The effect of protonation events on the charge equilibrium between tyrosine-D and the water-oxidizing complex in photosystem II has been studied by time-resolved measurements of the EPR signal IIslow at room temperature. The flash-induced oxidation of YD by the water-oxidizing complex in the S2 state is a monophasic p...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00217a037

    authors: Vass I,Styring S

    更新日期:1991-01-22 00:00:00

  • Thermodynamic characterization of the binding interaction between the histone demethylase LSD1/KDM1 and CoREST.

    abstract::Flavin-dependent histone demethylases catalyze the posttranslational oxidative demethylation of mono- and dimethylated lysine residues, producing formaldehyde and hydrogen peroxide in addition to the corresponding demethylated protein. In vivo, histone demethylase LSD1 (KDM1; BCH110) is a component of the multiprotein...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi101776t

    authors: Hwang S,Schmitt AA,Luteran AE,Toone EJ,McCafferty DG

    更新日期:2011-02-01 00:00:00

  • Noncompetitive Allosteric Antagonism of Death Receptor 5 by a Synthetic Affibody Ligand.

    abstract::Fatty acid-induced upregulation of death receptor 5 (DR5) and its cognate ligand, tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), promotes hepatocyte lipoapoptosis, which is a key mechanism in the progression of fatty liver disease. Accordingly, inhibition of DR5 signaling represents an attractive str...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/acs.biochem.0c00529

    authors: Vunnam N,Szymonski S,Hirsova P,Gores GJ,Sachs JN,Hackel BJ

    更新日期:2020-10-13 00:00:00

  • Molecular mechanisms and binding site location for the noncompetitive antagonist crystal violet on nicotinic acetylcholine receptors.

    abstract::We investigated the molecular mechanisms and the binding site location for the fluorophor crystal violet (CrV), a noncompetitive antagonist of the nicotinic acetylcholine receptor (AChR). To this end, radiolabeled competition binding, fluorescence spectroscopy, Schild-type analysis, patch-clamp recordings, and molecul...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi051752e

    authors: Arias HR,Bhumireddy P,Spitzmaul G,Trudell JR,Bouzat C

    更新日期:2006-02-21 00:00:00

  • Tritium isotope effects in adenosylcobalamin-dependent methylmalonyl-CoA mutase.

    abstract::Methylmalonyl-CoA mutase from Propionibacterium shermanii is an adenosylcobalamin-dependent enzyme which catalyzes the reversible isomerization of methylmalonyl-CoA and succinyl-CoA. The rate of tritium loss from 5'-[3H]adenosylcobalamin during the enzymic reaction and the relative rates of tritium appearance in subst...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi961250o

    authors: Meier TW,Thomä NH,Leadlay PF

    更新日期:1996-09-10 00:00:00

  • Enzymatic properties of proteolytic derivatives of human alpha-thrombin.

    abstract::The use of derivatives of alpha-thrombin obtained by limited proteolysis, that have only a single peptide bond cleaved, allowed the unequivocal correlation between the change in covalent structure and alteration of the enzymatic properties. beta T-Thrombin contains a single cleavage in the surface loop corresponding t...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00406a049

    authors: Hofsteenge J,Braun PJ,Stone SR

    更新日期:1988-03-22 00:00:00

  • Use of pH and kinetic isotope effects to establish chemistry as rate-limiting in oxidation of a peptide substrate by LSD1.

    abstract::The mechanism of oxidation of a peptide substrate by the flavoprotein lysine-specific demethylase (LSD1) has been examined using the effects of pH and isotopic substitution on steady-state and rapid-reaction kinetic parameters. The substrate contained the 21 N-terminal residues of histone H3, with a dimethylated lysyl...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi900499w

    authors: Gaweska H,Henderson Pozzi M,Schmidt DM,McCafferty DG,Fitzpatrick PF

    更新日期:2009-06-16 00:00:00

  • Rearrangement of domain elements of the Ca-ATPase in cardiac sarcoplasmic reticulum membranes upon phospholamban phosphorylation.

    abstract::Phospholamban (PLB) is a major target of the beta-adrenergic cascade in the heart, and functions to modulate rate-limiting conformational transitions involving the transport activity of the Ca-ATPase. To investigate structural changes within the Ca-ATPase that result from the phosphorylation of PLB by cAMP-dependent p...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi990599j

    authors: Negash S,Huang S,Squier TC

    更新日期:1999-06-22 00:00:00

  • Generality of the NUX rule: kinetic analysis of the results of systematic mutations in the trinucleotide at the cleavage site of hammerhead ribozymes.

    abstract::In order to study in detail the generality of the NUX (N = A, U, G, or C; X = A, U, or C) rule for the GUC triplet adjacent to the cleavage site in hammerhead ribozymes, two kinetic parameters, namely, kcat and Km, were determined for substrates with mutations in this triplet, which included double mutants with mutati...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00011a020

    authors: Shimayama T,Nishikawa S,Taira K

    更新日期:1995-03-21 00:00:00

  • The N-terminus of the fragile X mental retardation protein contains a novel domain involved in dimerization and RNA binding.

    abstract::Fragile X syndrome, the most common cause of inherited mental retardation, is caused by the absence of the fragile X mental retardation protein (FMRP). The emerging picture is that FMRP is involved in repression of translation through a complex network of protein-protein and protein-RNA interactions. Very little struc...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi034909g

    authors: Adinolfi S,Ramos A,Martin SR,Dal Piaz F,Pucci P,Bardoni B,Mandel JL,Pastore A

    更新日期:2003-09-09 00:00:00

  • Enhancement of Agkistrodon piscivorus piscivorus venom phospholipase A2 activity toward phosphatidylcholine vesicles by lysolecithin and palmitic acid: studies with fluorescent probes of membrane structure.

    abstract::The activity of phospholipase A2 from snake venom to hydrolyze bilayers of phosphatidylcholines is greatly enhanced by the presence of the hydrolysis products, lysolecithin and fatty acid, in the bilayer. The fluorescence of several probes of membrane structure was used to monitor changes in bilayer physical propertie...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00024a003

    authors: Sheffield MJ,Baker BL,Li D,Owen NL,Baker ML,Bell JD

    更新日期:1995-06-20 00:00:00

  • Mutation at active site lysine 212 to arginine uncouples the glycosylase activity from the lyase activity of human endonuclease III.

    abstract::The human endonuclease III (hNTH1) is an important DNA glycosylase with associated abasic lyase activity. We previously demonstrated that the K212Q mutant was totally inactive, while the K212R mutant had reduced DNA glycosylase/lyase activity and could form a covalent complex with the substrate DNA upon reduction. We ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi011053b

    authors: Liu X,Roy R

    更新日期:2001-11-13 00:00:00

  • Genome-Wide Mapping of Oxidative DNA Damage via Engineering of 8-Oxoguanine DNA Glycosylase.

    abstract::The occurrence of 8-oxo-7,8-dihydroguanine (OG) in the genome, as one of the major DNA oxidative damages, has been implicated in an array of biological processes, ranging from mutagenesis to transcriptional regulation. Genome-wide mapping of oxidative damages could shed light on the underlying cellular mechanism. In t...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/acs.biochem.9b00782

    authors: Fang Y,Zou P

    更新日期:2020-01-14 00:00:00