Comparative studies on the 7-iodo and 7-fluoro derivatives of N-acetoxy-N-2-acetylaminofluorene: binding sites on DNA and conformational change of modified deoxytrinucleotides.

abstract：:The generation of multiprotein complexes at receptors and adapter proteins is crucial for the activation of intracellular signaling pathways. In this study, we used multiple biochemical and biophysical methods to examine the binding properties of several SH2 and SH3 domain-containing signaling proteins as they interac...

journal_title：Biochemistry

authors： Houtman JC,Higashimoto Y,Dimasi N,Cho S,Yamaguchi H,Bowden B,Regan C,Malchiodi EL,Mariuzza R,Schuck P,Appella E,Samelson LE

更新日期：2004-04-13 00:00:00

abstract：:Strong absorbance of water at 1645 cm-1 has previously prevented dependable observation of infrared spectra of H2O solutions in the carbonyl region. We have obtained reliable spectra in pure water and in alcohol-water mixtures in this region and used them to study A.U polynucleotide interaction. Spectra in pure water ...

journal_title：Biochemistry

authors： Miles HT,Frazier J

更新日期：1978-07-11 00:00:00

abstract：:The fidelity of the poliovirus RNA-dependent RNA polymerase (3D(pol)) plays a direct role in the genomic evolution and pathogenesis of the virus. A single site mutation (Gly64Ser) that is remote from the catalytic center results in a higher fidelity polymerase. NMR studies with [methyl-(13)C]methionine-labeled protein...

journal_title：Biochemistry

authors： Yang X,Welch JL,Arnold JJ,Boehr DD

更新日期：2010-11-02 00:00:00

abstract：:Structurally diverse small molecules, including 5-(2-benzofuryl)-4-phenyl-1,2,4-triazole-3-thiol (BETT), have been identified via high-throughput screening as activators of caspase-3 in HeLa cell extracts. However, little is known about their mechanism of action. In this study, we investigate how BETT regulates prothy...

journal_title：Biochemistry

authors： Qi X,Wang L,Du F

更新日期：2010-03-09 00:00:00

abstract：:Protein-protein interactions mediate the vast majority of cellular processes. Though protein interactions obey basic chemical principles also within the cell, the in vivo physiological environment may not allow for equilibrium to be reached. Thus, in vitro measured thermodynamic affinity may not provide a complete pic...

journal_title：Biochemistry

authors： Cohen-Khait R,Schreiber G

更新日期：2018-08-07 00:00:00

abstract：:We studied the basic steps of proton transfer through ATP synthase of chloroplasts, CF0CF1, under conditions of proton slip, a conducting stage in the absence of added nucleotides. On the background of a steady transmembrane pH difference, voltage steps were induced by flashing light. Proton intake, transfer, and rele...

journal_title：Biochemistry

authors： Groth G,Junge W

更新日期：1995-07-11 00:00:00

abstract：:In search of the regulation mechanisms for isoform specific myosin assembly, we have used the COOH-terminal fragments of nonmuscle myosin isoforms MIIA and MIIB (MIIA(F46) and MIIB(alpha)(F47)) as a model system. Phosphorylation by protein kinase C (PK C) or casein kinase II (CK II) within or near the nonhelical tail-...

journal_title：Biochemistry

authors： Murakami N,Kotula L,Hwang YW

更新日期：2000-09-19 00:00:00

abstract：:The spin label Tempo-maleimide, when "immobilized" in hemoglobin, is shown to exhibit motional fluctuation whose amplitude and/or frequency depend on temperature and solution conditions. These motional fluctuations are observable by several electron spin resonance techniques. For desalted hemoglobin the fluctuations a...

journal_title：Biochemistry

authors： Johnson ME

更新日期：1978-04-04 00:00:00

abstract：:DNA polymerase (pol) β is a small eukaryotic DNA polymerase composed of two domains. Each domain contributes an enzymatic activity (DNA synthesis and deoxyribose phosphate lyase) during the repair of simple base lesions. These domains are termed the polymerase and lyase domains, respectively. Pol β has been an excelle...

journal_title：Biochemistry

authors： Beard WA,Wilson SH

更新日期：2014-05-06 00:00:00

abstract：:P-450(15)alpha is a form of cytochrome P-450 purified from liver microsomes of female 129/J mice that is specific for oxidation of testosterone to its 15 alpha-hydroxylated product. Testosterone 15 alpha-hydroxylase activity that was inhibited by anti-P-450(15)alpha antibody was approximately 50 times higher in renal ...

journal_title：Biochemistry

authors： Squires EJ,Negishi M

更新日期：1986-08-26 00:00:00

abstract：:Binding of urokinase-type plasminogen activator (uPA) to its cellular receptor (uPAR) renders the cell surface a favored site for plasminogen activation. Recently, a 15-mer peptide antagonist of the uPA-uPAR interaction, with an IC50 value of 10 nM, was identified using phage display technology [Goodson, R. J., Doyle,...

journal_title：Biochemistry

authors： Ploug M,Ostergaard S,Hansen LB,Holm A,Danø K

更新日期：1998-03-17 00:00:00

abstract：:The committed step in menaquinone biosynthesis is the formation of o-succinylbenzoate (OSB). It is presumed to require the reaction of a seven-carbon intermediate of the shikimate pathway with a succinic semialdehyde-thiamin pyrophosphate (TPP) anion, derived by decarboxylation of 2-ketoglutarate. The following eviden...

journal_title：Biochemistry

authors： Marley MG,Meganathan R,Bentley R

更新日期：1986-03-25 00:00:00

abstract：:Two scorpion neurotoxins active only on insects, the insect toxins AaH IT1 and AaH IT2, were purified from the venom of scorpions Androctonus australis Hector collected in Tozeur (Tunisia) and characterized. AaH IT2 was sequenced and found to differ in four amino acid positions from AaH IT, the single previously seque...

journal_title：Biochemistry

authors： Loret EP,Mansuelle P,Rochat H,Granier C

更新日期：1990-02-13 00:00:00

abstract：:The cell-surface component (alpha) which binds monomeric immunoglobulin E with high affinity is associated with a second polypeptide (beta) in the plasma membrane. The latter component tends to dissociate during purification of the alpha chain from detergent extracts of cells, even at neutral pHs and physiological ion...

journal_title：Biochemistry

authors： Rivnay B,Wank SA,Poy G,Metzger H

更新日期：1982-12-21 00:00:00

abstract：:Three spin-labeled derivatives of the voltage-gated peptide alamethicin were prepared with nitroxides at the C-terminal phenyalaninol, and at positions 9 and 15 in the amino acid sequence. In addition, three spin-labeled derivatives of an analog of alamethicin where alpha-methylalanine residues are replaced by leucine...

journal_title：Biochemistry

authors： Barranger-Mathys M,Cafiso DS

更新日期：1996-01-16 00:00:00

abstract：:The interaction between eosin-5-maleimide (EMA), an inhibitor of the anion transport protein, band 3, and I-, a transportable substrate, was investigated by fluorescence quenching. The Stern-Volmer plot for the quenching reaction between EMA-labeled band 3 and I- exhibits downward curvature both in human erythrocyte g...

journal_title：Biochemistry

authors： Pan RJ,Cherry RJ

更新日期：1995-04-11 00:00:00

abstract：:The cytochrome d complex is a two-subunit, membrane-bound terminal oxidase in the aerobic respiratory chain of Escherichia coli. The enzyme catalyzes the two-electron oxidation of ubiquinol and the four-electron reduction of oxygen to water. Previous work demonstrated that the site for ubiquinol oxidation was selectiv...

journal_title：Biochemistry

authors： Dueweke TJ,Gennis RB

更新日期：1991-04-09 00:00:00

abstract：:Adenylosuccinate lyase of Bacillus subtilis is inactivated by 2-[(4-bromo-2,3-dioxobutyl)thio]adenosine 5'-monophosphate (2-BDB-TAMP) at pH 7.0. As the reagent concentration is increased, a maximum rate constant is approached, indicative of reversible enzyme-reagent complex formation (KR = 68 +/- 9 microM) prior to ir...

journal_title：Biochemistry

authors： Lee TT,Worby C,Bao ZQ,Dixon JE,Colman RF

更新日期：1998-06-09 00:00:00

abstract：:A preparation consisting of isolated dimeric peripheral antenna complexes from green plant photosystem I (light-harvesting complex I or LHCI) has been characterized by means of (polarized) steady-state absorption and fluorescence spectroscopy at low temperatures. We show that this preparation can be described reasonab...

journal_title：Biochemistry

authors： Ihalainen JA,Gobets B,Sznee K,Brazzoli M,Croce R,Bassi R,van Grondelle R,Korppi-Tommola JE,Dekker JP

更新日期：2000-07-25 00:00:00

abstract：:The Stk1/Stp1 and GraSR signal-transduction pathways are two distinct pathways in Staphylococcus aureus that rely on a reversible phosphorylation process in transducing external stimuli intracellularly. Stk1/Stp1 is an eukaryote-like Ser/Thr kinase phosphatase pair involved in purine biosynthesis, cell-wall metabolism...

journal_title：Biochemistry

authors： Fridman M,Williams GD,Muzamal U,Hunter H,Siu KW,Golemi-Kotra D

更新日期：2013-11-12 00:00:00

abstract：:GTPases are key players during cellular signaling. Phosphorylation of Rab proteins, which belong to the Ras superfamily of small GTPases regulating intracellular transport, has been implicated in the pathogenesis of Parkinson's disease. For Rab8a, it was shown that serine 111 phosphorylation (pS111) is dependent on th...

journal_title：Biochemistry

authors： Pourjafar-Dehkordi D,Vieweg S,Itzen A,Zacharias M

更新日期：2019-08-20 00:00:00

abstract：:To test the prediction that extensively fluorinated (fluorous) proteins should be more stable and exhibit novel self-segregating behavior, the properties of the de novo designed model 4-alpha-helix bundle protein, alpha 4F 6, in which the hydrophobic core is packed entirely with the extensively fluorinated amino acid ...

journal_title：Biochemistry

authors： Gottler LM,de la Salud-Bea R,Marsh EN

更新日期：2008-04-15 00:00:00

abstract：:The role of bacterial DnaJ protein as a cochaperone of DnaK is strongly appreciated. Although DnaJ unaccompanied by DnaK can bind unfolded as well as native substrate proteins, its role as an individual chaperone remains elusive. In this study, we demonstrate that DnaJ binds a model non-native substrate with a low nan...

journal_title：Biochemistry

authors： Tiwari S,Kumar V,Jayaraj GG,Maiti S,Mapa K

更新日期：2013-02-12 00:00:00

abstract：:Bactenecin 5 and its fragments [BN22 (1-22), BN16 (7-22), and BC24 (20-43)] were synthesized by solid-phase methods. Their antifungal activities on Candida albicans have been studied and compared with those of the native bactenecin 5. The conformational preferences of these peptides in aqueous and nonaqueous solutions...

journal_title：Biochemistry

authors： Raj PA,Marcus E,Edgerton M

更新日期：1996-04-09 00:00:00

abstract：:The intestinal fatty acid binding protein is composed of two beta-sheets surrounding a large interior cavity. There is a small helical domain associated with the portal for entry of the ligand into the cavity. Denaturation of the protein has been monitored in a residue-specific manner by collecting a series of two-dim...

journal_title：Biochemistry

authors： Hodsdon ME,Frieden C

更新日期：2001-01-23 00:00:00

abstract：:Coumermycin A(1) is a member of the aminocoumarin family of antibiotics. Unlike its structural relatives, novobiocin and clorobiocin, coumermycin A(1) is a dimer built on a 3-methyl-2,4-dicarboxypyrrole scaffold and bears two decorated noviose sugar components which are the putative target binding motifs for DNA gyras...

journal_title：Biochemistry

authors： Freel Meyers CL,Oberthür M,Heide L,Kahne D,Walsh CT

更新日期：2004-11-30 00:00:00

abstract：:We have used circular dichroism and frequency-domain fluorescence spectroscopy to determine how the site-specific substitution of Tyr138 with either Phe138 or Gln138 affects the structural coupling between the opposing domains of calmodulin (CaM). A double mutant was constructed involving conservative substitution of ...

journal_title：Biochemistry

authors： Sun H,Yin D,Coffeen LA,Shea MA,Squier TC

更新日期：2001-08-14 00:00:00

abstract：:Crystal structures of complexes of a D30N mutant of feline immunodeficiency virus protease (FIV PR) complexed with a statine-based inhibitor (LP-149), as well as with a substrate based on a modification of this inhibitor (LP-149S), have been solved and refined at resolutions of 2.0 and 1.85 A, respectively. Both the i...

journal_title：Biochemistry

authors： Laco GS,Schalk-Hihi C,Lubkowski J,Morris G,Zdanov A,Olson A,Elder JH,Wlodawer A,Gustchina A

更新日期：1997-09-02 00:00:00

abstract：:Helicobacter pylori NikR (HpNikR) is a nickel-responsive transcription factor. In addition to a role in nickel homeostasis, HpNikR is proposed to serve as a master activator-repressor for H. pylori acid adaptation by directly or indirectly regulating the expression of a battery of genes. One potential mechanism of thi...

journal_title：Biochemistry

authors： Li Y,Zamble DB

更新日期：2009-03-24 00:00:00

abstract：:The effect of DNA supercoiling on a sterically very demanding threading intercalation process is investigated here. We find that the threading rate of a dimeric ruthenium complex into a negatively supercoiled plasmid at low binding density is 2 orders of magnitude higher than into the cleaved linear form. Further satu...

journal_title：Biochemistry

authors： Nordell P,Jansson ET,Lincoln P

更新日期：2009-02-24 00:00:00