Comparative studies on the 7-iodo and 7-fluoro derivatives of N-acetoxy-N-2-acetylaminofluorene: binding sites on DNA and conformational change of modified deoxytrinucleotides.


:GMP and native DNA were reacted with 7-iodo and 7-fluoro derivatives of N-acetoxy-N-2-acetylaminofluorene. It was shown that the 7-halogeno derivatives react on C-8 of guanine. Furthermore the respective amount of arylamidation (covalent linkage on the C-8 of guanine) and arylation (covalent linkage on 2-NH2 groups of C3 of guanine) addition products was determined in both native and denatured DNA-[14C]AAIF. Two G containing deoxytrinucleotides modified by either AAFF or AAIF were studied comparatively by means of circular dichroism, and as a function of several parameters known to affect the conformation of the deoxytrinucleotides. The induced optical activity in fluorofluorene ring seemed to be very sensible to the conformational changes of the deoxytrinucleotides. On the other hand, the AAIF residue exhibit a lower induced optical activity which remained unchanged when the deoxytrinucleotides conformation was affected. The results presented in this paper led us to conclude that the AAFF and AAIF modified deoxytrinucleotides adopt a conformation which nicely fits with the insertion-denaturation and outside-binding model, respectively.






Lefèvre JF,Fuchs RP,Daune MP




Has Abstract


1978-06-27 00:00:00












  • Cross-linking of a monomeric 39/34-kDa dispase fragment of von Willebrand factor (Leu-480/Val-481-Gly-718) to the N-terminal region of the alpha-chain of membrane glycoprotein Ib on intact platelets with bis(sulfosuccinimidyl) suberate.

    abstract::A 39/34-kilodalton (kDa) monomeric dispase fragment of von Willebrand factor (vWF) has been purified by heparin affinity chromatography. Detailed structural analysis of the individual 39- and 34-kDa fragments indicated that they had identical amino acid sequences extending from Leu-480/Val-481 to Gly-718 with an intra...


    pub_type: 杂志文章


    authors: Andrews RK,Gorman JJ,Booth WJ,Corino GL,Castaldi PA,Berndt MC

    更新日期:1989-10-17 00:00:00

  • Identification and structural characterization of an unusual RING-like sequence within an extracellular biomineralization protein, AP7.

    abstract::The RING or Really Interesting New Gene represents a family of eukaryotic sequences that bind Zn (II) ions and participate in intracellular processes involving protein-protein interaction. Although found in over 400 different proteins, very little is known regarding the structure-function properties of these domains b...


    pub_type: 杂志文章


    authors: Collino S,Kim IW,Evans JS

    更新日期:2008-03-25 00:00:00

  • Location and interactions of phospholipid and cholesterol in human low density lipoprotein from 31P nuclear magnetic resonance.

    abstract::The major phospholipids, phosphatidylcholine and spingomyelin, of low density lipoprotein (LDL) are accessible to small amounts of Pr3+, suggesting that the head groups of all mobile phospholipids are on the surface of the particle in contact with the aqueous medium. The major source of the nuclear Overhauser effect e...


    pub_type: 杂志文章


    authors: Yeagle PL,Martin RB,Pottenger L,Langdon RG

    更新日期:1978-07-11 00:00:00

  • Mechanism of 25-hydroxyvitamin D3 24-hydroxylation: incorporation of oxygen-18 into the 24 position of 25-hydroxyvitamin D3.

    abstract::The oxygen enzymatically inserted as a hydroxyl function by chick kidney homogenate into the 24 position of 25-hydroxyvitamin D3 to give 24,25-dihydroxyvitamin D3 is derived exclusively from 18O2. Therefore, like the 25-hydroxyvitamin D3-lalpha-hydroxylase system, the 25-hydroxyvitamin D3-24-hydroxylase system is also...


    pub_type: 杂志文章


    authors: Madhok TC,Schnoes HK,DeLuca HF

    更新日期:1977-05-17 00:00:00

  • Kinetics of long-chain (sphingoid) base biosynthesis in intact LM cells: effects of varying the extracellular concentrations of serine and fatty acid precursors of this pathway.

    abstract::Serine palmitoyltransferase (EC catalyzes the condensation of L-serine and palmitoyl-CoA to yield 3-ketosphinganine in the first unique reaction of long-chain (sphingoid) base biosynthesis. The kinetic effects of changing the extracellular concentrations of the precursors for this pathway were studied with L...


    pub_type: 杂志文章


    authors: Merrill AH Jr,Wang E,Mullins RE

    更新日期:1988-01-12 00:00:00

  • Peptide cross-linking to calmodulin: attachment of [Tyr8]substance P.

    abstract::Ultraviolet irradiation of calmodulin in the presence of calcium results in either the intramolecular cross-linking of Tyr99 and Tyr138 [Malencik, D.A., & Anderson, S.R. (1986) Biochemistry 25, 709] or, when [Tyr8]substance P is bound, the generation of peptide-calmodulin adducts. The latter consist of two chromatogra...


    pub_type: 杂志文章


    authors: Malencik DA,Anderson SR

    更新日期:1988-02-09 00:00:00

  • Characterization of the collagens synthesized by Chinese hamster ovary cells. Effect of colcemid and dibutyryladenosine cyclic monophosphate.

    abstract::The collagens synthesized by Chinese hamster ovary cells have been isolated and characterized. Although these cells produce very small amounts of collagen, at least five distinct collagenous chains could be identified from radiolabeled media and cell extracts after limited pepsin digestion. Two chains were characteriz...


    pub_type: 杂志文章


    authors: Limeback H,Sodek J,Aubin J

    更新日期:1982-09-14 00:00:00

  • Properties of the high-affinity single-stranded DNA binding state of the Escherichia coli recA protein.

    abstract::The properties of the high-affinity single-stranded DNA (ssDNA) binding state of Escherichia coli recA protein have been studied. We find that all of the nucleoside triphosphates that are hydrolyzed by recA protein induce a high-affinity ssDNA binding state. The effect of ATP binding to recA protein was partially sepa...


    pub_type: 杂志文章


    authors: Menetski JP,Varghese A,Kowalczykowski SC

    更新日期:1988-02-23 00:00:00

  • Binding isotherms of nucleosides and polynucleotides measured by continuous ultrafiltration at constant volume: a rapid and precise technique.

    abstract::Equilibrium binding isotherms have been measured as a function of temperature for the interaction of 2- and 8-aminoadenosine with poly(U) by continuous, constant-volume ultrafiltration. An analytical method is described for determining the entire isotherm in a single experiment on a preequilibrated ligand--polymer sol...


    pub_type: 杂志文章


    authors: Roy KB,Miles HT

    更新日期:1982-01-05 00:00:00

  • Binding of ncd to microtubules induces a conformational change near the junction of the motor domain with the neck.

    abstract::We have covalently attached an electron paramagnetic resonance (EPR) spin probe to Cys-670 of the motor domain of ncd (nonclaret disjunctional protein) in order to investigate conformational changes associated with the chemomechanical cycle. Spin-labeling is highly specific and does not affect ncd function as monitore...


    pub_type: 杂志文章


    authors: Naber N,Cooke R,Pate E

    更新日期:1997-08-12 00:00:00

  • Protease Inhibition Mechanism of Camelid-like Synthetic Human Antibodies.

    abstract::Macromolecular protease inhibitors and camelid single-domain antibodies achieve their enzymic inhibition functions often through protruded structures that directly interact with catalytic centers of targeted proteases. Inspired by this phenomenon, we constructed synthetic human antibody libraries encoding long CDR-H3s...


    pub_type: 杂志文章


    authors: Nam DH,Lee KB,Kruchowy E,Pham H,Ge X

    更新日期:2020-10-13 00:00:00

  • Physical mechanism for regulation of phosphoenolpyruvate-dependent glucose transport activity in Escherichia coli.

    abstract::The activity of the phosphoenolpyruvate-dependent glucose phosphotransferase system (PTS) in Escherichia coli is coupled to the oxidation-reduction potential. It is inhibited when the redox potential is increased above -300 mV either via substrate oxidation or via direct addition of oxidizing agents. Depending on the ...


    pub_type: 杂志文章


    authors: Robillard GT,Konings WN

    更新日期:1981-08-18 00:00:00

  • Local conformational transition of Hydrogenobacter thermophilus cytochrome c552 relevant to its redox potential.

    abstract::In order to elucidate the molecular mechanisms responsible for the apparent nonlinear behavior of the temperature dependence of the redox potential of Hydrogenobacter thermophilus cytochrome c552 [Takahashi, Y., Sasaki, H., Takayama, S. J., Mikami, S., Kawano, S., Mita, H., Sambongi, Y., and Yamamoto, Y. (2006) Bioche...


    pub_type: 杂志文章


    authors: Takayama SJ,Takahashi YT,Mikami S,Irie K,Kawano S,Yamamoto Y,Hemmi H,Kitahara R,Yokoyama S,Akasaka K

    更新日期:2007-08-14 00:00:00

  • Resonance Raman evidence for the presence of two heme pocket conformations with varied activities in CO-bound bovine soluble guanylate cyclase and their conversion.

    abstract::Resonance Raman (RR) spectra of soluble guanylate cyclase (sGC) reported by five independent research groups have been classified as two types: sGC(1) and sGC(2). Here we demonstrate that the RR spectra of sGC isolated from bovine lung contain only sGC(2) while both species are observed in the spectra of the CO-bound ...


    pub_type: 杂志文章


    authors: Li Z,Pal B,Takenaka S,Tsuyama S,Kitagawa T

    更新日期:2005-01-25 00:00:00

  • A potent oligosaccharyl transferase inhibitor that crosses the intracellular endoplasmic reticulum membrane.

    abstract::Recent work has resulted in the development of potent inhibitors of oligosaccharyl transferase (OT), the enzyme that catalyzes the cotranslational glycosylation of asparagine [Hendrickson, T. L., Spencer, J. R., Kato, M., and Imperiali, B. (1996) J. Am. Chem. Soc. 118, 7636-7637; Kellenberger, C., Hendrickson, T. L., ...


    pub_type: 杂志文章


    authors: Eason PD,Imperiali B

    更新日期:1999-04-27 00:00:00

  • Conservation of electrostatic properties within enzyme families and superfamilies.

    abstract::Electrostatic interactions play a key role in enzyme catalytic function. At long range, electrostatics steer the incoming ligand/substrate to the active site, and at short distances, electrostatics provide the specific local interactions for catalysis. In cases in which electrostatics determine enzyme function, orthol...


    pub_type: 杂志文章


    authors: Livesay DR,Jambeck P,Rojnuckarin A,Subramaniam S

    更新日期:2003-04-01 00:00:00

  • Detection of RNA-Protein Interactions in Living Cells with SHAPE.

    abstract::SHAPE-MaP is unique among RNA structure probing strategies in that it both measures flexibility at single-nucleotide resolution and quantifies the uncertainties in these measurements. We report a straightforward analytical framework that incorporates these uncertainties to allow detection of RNA structural differences...


    pub_type: 杂志文章


    authors: Smola MJ,Calabrese JM,Weeks KM

    更新日期:2015-11-24 00:00:00

  • Thermodynamic characterization of the osmolyte- and ligand-folded states of Bacillus subtilis ribonuclease P protein.

    abstract::In Bacillus subtilis, P protein is the noncatalytic component of ribonuclease P (RNase P) that is critical for achieving maximal nuclease activity under physiological conditions. P protein is predominantly unfolded (D) at neutral pH and low ionic strength; however, it folds upon the addition of sulfate anions (ligands...


    pub_type: 杂志文章


    authors: Henkels CH,Oas TG

    更新日期:2005-10-04 00:00:00

  • Human secreted carbonic anhydrase: cDNA cloning, nucleotide sequence, and hybridization histochemistry.

    abstract::Complementary DNA clones coding for the human secreted carbonic anhydrase isozyme (CA VI) have been isolated and their nucleotide sequences determined. These clones identify a 1.45-kb mRNA that is present in high levels in parotid submandibular salivary glands but absent in other tissues such as the sublingual gland, ...


    pub_type: 杂志文章


    authors: Aldred P,Fu P,Barrett G,Penschow JD,Wright RD,Coghlan JP,Fernley RT

    更新日期:1991-01-15 00:00:00

  • A reconstituted light-harvesting complex from the green sulfur bacterium Chlorobium tepidum containing CsmA and bacteriochlorophyll a.

    abstract::Green sulfur bacteria possess two light-harvesting antenna systems, the chlorosome and the Fenna-Matthews-Olson (FMO) protein. In addition to self-aggregated bacteriochlorophyll (BChl) c, chlorosomes of Chlorobium tepidum contain a small amount of BChl a (ratio 100:1). The chlorosomal BChl a is associated with CsmA, a...


    pub_type: 杂志文章


    authors: Pedersen MØ,Pham L,Steensgaard DB,Miller M

    更新日期:2008-02-05 00:00:00

  • DNA cross-linking and sequence selectivity of aziridinylbenzoquinones: a unique reaction at 5'-GC-3' sequences with 2,5-diaziridinyl-1,4-benzoquinone upon reduction.

    abstract::Several bifunctional alkylating agents of the aziridinylbenzoquinone class have been evaluated as potential antitumor agents. 3,6-Bis[(2-hydroxyethyl)amino]-2,5- diaziridinyl-1,4-benzoquinone (BZQ), 2,5-diaziridinyl-1,4-benzoquinone (DZQ), 3,6-bis(carboxyamino)-2,5-diaziridinyl- 1,4-benzoquinone (AZQ), and six analogu...


    pub_type: 杂志文章


    authors: Hartley JA,Berardini M,Ponti M,Gibson NW,Thompson AS,Thurston DE,Hoey BM,Butler J

    更新日期:1991-12-17 00:00:00

  • Mechanistic studies examining the efficiency and fidelity of DNA synthesis by the 3TC-resistant mutant (184V) of HIV-1 reverse transcriptase.

    abstract::A single amino acid substitution from methionine-184 to valine (M184V) of HIV-1 reverse transcriptase (RT) evokes the 1000-fold 3TC (Lamivudine) resistance by the HIV-1 virus observed in the clinic. The M184V mutant HIV-1 RT was studied to assess its catalytic efficiency during single nucleotide incorporation using a ...


    pub_type: 杂志文章


    authors: Feng JY,Anderson KS

    更新日期:1999-07-20 00:00:00

  • Inactivation of steroid sulfatase by an active site-directed inhibitor, estrone-3-O-sulfamate.

    abstract::Steroid sulfatases are responsible for the hydrolysis of 3beta-hydroxy steroid sulfates, such as cholesterol and pregnenolone sulfate, and have an important role in regulating the synthesis of estrogenic steroids, from estrone sulfate and dehydroepiandrosterone sulfate, in endocrine-dependent tumors. Although little i...


    pub_type: 杂志文章


    authors: Purohit A,Williams GJ,Howarth NM,Potter BV,Reed MJ

    更新日期:1995-09-12 00:00:00

  • Identification and mechanism of a bacterial hydrolyzing UDP-N-acetylglucosamine 2-epimerase.

    abstract::This paper reports the first identification of a fully functional hydrolyzing UDP-N-acetylglucosamine 2-epimerase from a bacterial source. The epimerase (known as SiaA or NeuC) from Neisseria meningitidis MC58 group B is shown to catalyze the conversion of UDP-GlcNAc into ManNAc and UDP in the first step of sialic aci...


    pub_type: 杂志文章


    authors: Murkin AS,Chou WK,Wakarchuk WW,Tanner ME

    更新日期:2004-11-09 00:00:00

  • Identification of the sites phosphorylated by cyclic AMP-dependent protein kinase on the beta 2 subunit of L-type voltage-dependent calcium channels.

    abstract::Voltage-dependent L-type calcium (Ca) channels are heteromultimeric proteins that are regulated through phosphorylation by cAMP-dependent protein kinase (PKA). We demonstrated that the beta 2 subunit was a substrate for PKA in intact cardiac myocytes through back-phosphorylation experiments. In addition, a heterologou...


    pub_type: 杂志文章


    authors: Gerhardstein BL,Puri TS,Chien AJ,Hosey MM

    更新日期:1999-08-10 00:00:00

  • Vinca site agents induce structural changes in tubulin different from and antagonistic to changes induced by colchicine site agents.

    abstract::Vinca site agents are antimicrotubule compounds that bind to the same site on tubulin as do the vinca alkaloids. These include agents that induce the formation of nonmicrotubule oligomers of tubulin (vinblastine and vincristine) and agents that do not (maytansine and rhizoxin). All of these quench the fluorescence of ...


    pub_type: 杂志文章


    authors: Sackett DL

    更新日期:1995-05-30 00:00:00

  • Secretory carrier membrane protein SCAMP2 and phosphatidylinositol 4,5-bisphosphate interactions in the regulation of dense core vesicle exocytosis.

    abstract::Secretory carrier membrane protein 2 (SCAMP2) functions in late steps of membrane fusion in calcium-dependent granule exocytosis. A basic/hydrophobic peptide segment within SCAMP2 (SCAMP2 E: CWYRPIYKAFR) has been implicated in this function and shown to bind and sequester phosphatidylinositol 4,5-bisphosphate [PI(4,5)...


    pub_type: 杂志文章


    authors: Liao H,Ellena J,Liu L,Szabo G,Cafiso D,Castle D

    更新日期:2007-09-25 00:00:00

  • Dynamics of light-induced conformational changes of the phoborhodopsin/transducer complex formed in the n-dodecyl beta-D-maltoside micelle.

    abstract::A complex of photoreceptor phoborhodopsin (ppR; also called sensory rhodopsin II) and its cognate halobacterial transducer II (pHtrII) existing in the plasma membrane mediates the light signal to the cytoplasm in the earliest step of negative phototaxis in Natronomonas pharaonis. We have investigated the dynamics of t...


    pub_type: 杂志文章


    authors: Taniguchi Y,Ikehara T,Kamo N,Yamasaki H,Toyoshima Y

    更新日期:2007-05-08 00:00:00

  • Conformational consequences of ionization of Lys, Asp, and Glu buried at position 66 in staphylococcal nuclease.

    abstract::The pK(a) values measured previously for the internal Lys-66, Asp-66, and Glu-66 in variants of a highly stable form of staphylococcal nuclease are shifted by as many as 5 pK(a) units relative to normal pK(a) values in water. These shifts cannot be reproduced with continuum electrostatics calculations with static stru...


    pub_type: 杂志文章


    authors: Karp DA,Stahley MR,García-Moreno B

    更新日期:2010-05-18 00:00:00

  • Shutting Down Shigella Secretion: Characterizing Small Molecule Type Three Secretion System ATPase Inhibitors.

    abstract::Many important human pathogens rely on one or more type three secretion systems (T3SSs) to inject bacterial effector proteins directly into the host cell cytoplasm. Secretion of protein through the needlelike type three secretion apparatus (T3SA) is essential for pathogen virulence and relies on a highly conserved ATP...


    pub_type: 杂志文章


    authors: Case HB,Mattock DS,Dickenson NE

    更新日期:2018-12-18 00:00:00