Structure-activity relationships of piscidin 4, a piscine antimicrobial peptide.

Abstract:

:Piscidin 4, an antimicrobial peptide recently isolated from mast cells of hybrid striped bass (Morone chrysops female × Morone saxatilis male), is unusual in that it is twice as long (44 amino acids) as the typical members of the piscidin family. We previously showed that native piscidin 4 had a modified amino acid at position 20, but synthetic piscidin 4 (having an unmodified Trp at position 20) had similar potent activity against a number of both human and fish bacterial pathogens. In this study, the structure and membrane topology of synthetic piscidin 4 were examined using liposomes as model bilayers. Circular dichroism analyses revealed that it had a disordered structure in aqueous solution and folded to form a relatively weak α-helical structure in both membrane-mimetic trifluoroethanol solutions and liposome suspensions. Fluorescence data (piscidin 4 embedded in liposomes) and leakage experiments indicated that piscidin 4 interacted strongly with the hydrophobic part of the liposome. Binding of piscidin 4 to liposomes induced significant blue shifts of the emission spectra of the single Trp residue (Trp20). Quenching of Trp20 by water-soluble quencher (either acrylamide or I-) indicated that the fluorescence of Trp20 decreased more in the presence of liposomes than in buffer solution, thus revealing that Trp20 is less accessible to the quenchers in the presence of liposomes. The relative leakage abilities of piscidin 4 (1 μM) with liposomes were in the following order: DPPC (100%)≥EYPC (94%)>DPPC/DPPG (65%)>EYPC/EYPG (0%). This high activity against DPPC and EYPC liposomes was contrary to our data suggesting that piscidin 4 has a much weaker tendency to form an α-helix than other piscidins, such as piscidin 1. However, the structural similarity of protozoan membranes to EYPC liposomes might explain our discovery of the potent activity of piscidin 4 against the important skin/gill parasite ich (Ichthyophthirius multifiliis), but its negligible hemolytic activity against vertebrate membranes (hybrid striped bass or human erythrocytes). It also suggests that other conformation(s) in addition to the α-helix of this peptide may be responsible for its selective activity. This differential toxicity also suggests that piscidin 4 plays a significant role in the innate defense system of hybrid striped bass and may be capable of functioning extracellularly.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Park NG,Silphaduang U,Moon HS,Seo JK,Corrales J,Noga EJ

doi

10.1021/bi101395j

subject

Has Abstract

pub_date

2011-04-26 00:00:00

pages

3288-99

issue

16

eissn

0006-2960

issn

1520-4995

journal_volume

50

pub_type

杂志文章
  • Identification of multiple glucocorticoid receptor binding sites in the rat osteocalcin gene promoter.

    abstract::The biosynthesis of osteocalcin (OC), a bone-specific, noncollagenous protein, is stringently regulated during differentiation of the osteoblast phenotype. Glucocorticoids, and also 1,25(OH)2D3, mediate the developmental regulation of OC gene transcription. In this study, we established that the -1097 to +23 promoter ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00093a022

    authors: Heinrichs AA,Bortell R,Rahman S,Stein JL,Alnemri ES,Litwack G,Lian JB,Stein GS

    更新日期:1993-10-26 00:00:00

  • Serine protease of hepatitis C virus expressed in insect cells as the NS3/4A complex.

    abstract::Hepatitis C virus (HCV) protease NS3 and its protein activator NS4A participate in the processing of the viral polyprotein into its constituent nonstructural proteins. The NS3/4A complex is thus an attractive target for antiviral therapy against HCV. We expressed the full-length NS3 and NS4A in insect cells as a solub...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi972010r

    authors: Sali DL,Ingram R,Wendel M,Gupta D,McNemar C,Tsarbopoulos A,Chen JW,Hong Z,Chase R,Risano C,Zhang R,Yao N,Kwong AD,Ramanathan L,Le HV,Weber PC

    更新日期:1998-03-10 00:00:00

  • Small molecular, macromolecular, and cellular chloramines react with thiocyanate to give the human defense factor hypothiocyanite.

    abstract::Thiocyanate reacts noncatalytically with myeloperoxidase-derived HOCl to produce hypothiocyanite (OSCN(-)), thereby potentially limiting the propensity of HOCl to inflict host tissue damage that can lead to inflammatory diseases. However, the efficiency with which SCN(-) captures HOCl in vivo depends on the concentrat...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi902089w

    authors: Xulu BA,Ashby MT

    更新日期:2010-03-09 00:00:00

  • Detailed structural characterization of unbound protein phosphatase 1 inhibitors.

    abstract::Protein phosphatase 1 (PP1) is an essential and ubiquitous serine/threonine protein phosphatase that is regulated by more than 100 known inhibitor and targeting proteins. It is currently unclear how protein inhibitors distinctly and specifically regulate PP1 to enable rapid responses to cellular alterations. We demons...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi801308y

    authors: Dancheck B,Nairn AC,Peti W

    更新日期:2008-11-25 00:00:00

  • Structural Basis of Substrate Recognition by Aldehyde Dehydrogenase 7A1.

    abstract::Aldehyde dehydrogenase 7A1 (ALDH7A1) is part of lysine catabolism and catalyzes the NAD(+)-dependent oxidation of α-aminoadipate semialdehyde to α-aminoadipate. Herein, we describe a structural study of human ALDH7A1 focused on substrate recognition. Five crystal structures and small-angle X-ray scattering data are re...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/acs.biochem.5b00754

    authors: Luo M,Tanner JJ

    更新日期:2015-09-08 00:00:00

  • DNA strand arrangement within the SfiI-DNA complex: atomic force microscopy analysis.

    abstract::The SfiI restriction enzyme binds to DNA as a tetramer holding two usually distant DNA recognition sites together before cleavage of the four DNA strands. To elucidate structural properties of the SfiI-DNA complex, atomic force microscopy (AFM) imaging of the complexes under noncleaving conditions (Ca2+ instead of Mg2...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi051767c

    authors: Lushnikov AY,Potaman VN,Oussatcheva EA,Sinden RR,Lyubchenko YL

    更新日期:2006-01-10 00:00:00

  • Differential recognition of the free versus bound retinol by human microsomal retinol/sterol dehydrogenases: characterization of the holo-CRBP dehydrogenase activity of RoDH-4.

    abstract::All-trans-retinol is the precursor for all-trans-retinoic acid, the activating ligand for nuclear transcription factors retinoic acid receptors. In the cytosol of various cells, most retinol exists in a bound form, complexed with cellular retinol binding protein type I (holo-CRBP). Whether retinoic acid is produced fr...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi026836r

    authors: Lapshina EA,Belyaeva OV,Chumakova OV,Kedishvili NY

    更新日期:2003-01-28 00:00:00

  • One-disulfide intermediates of apamin exhibit native-like structure.

    abstract::The conformations of three peptide models of the one-disulfide and fully reduced forms of apamin were characterized by using nuclear magnetic resonance (NMR) spectroscopy. Apa-2 contains the native disulfide bond between Cys3 and Cys15 in apamin with the other two cysteines replaced by alanines. Apa-1 contains the nat...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00183a031

    authors: Xu X,Nelson JW

    更新日期:1994-05-03 00:00:00

  • Structural and thermodynamic characterization of Nore1-SARAH: a small, helical module important in signal transduction networks.

    abstract::Tumor suppressor Nore1, its acronym coming from novel Ras effector, is one of the 10 members of the Rassf (Ras association domain family) protein family that have been identified. It is expressed as two mRNA splice variants, Nore1A and a shorter isoform, Nore1B. It forms homo- and heterocomplexes through its C-termina...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi3014642

    authors: Makbul C,Constantinescu Aruxandei D,Hofmann E,Schwarz D,Wolf E,Herrmann C

    更新日期:2013-02-12 00:00:00

  • Electrophoretic mobility is a reporter of hairpin structure in single-stranded DNA oligomers.

    abstract::The electrophoretic mobilities of 24 single-stranded DNA oligomers, each containing 26 nucleotide residues, have been measured in polyacrylamide gels and in free solution. The mobilities observed at 20 degrees C differed by approximately 20% in polyacrylamide gels and by approximately 10% in free solution, even though...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi701058f

    authors: Stellwagen E,Abdulla A,Dong Q,Stellwagen NC

    更新日期:2007-09-25 00:00:00

  • Accumulation of long-chain acylcarnitine and 3-hydroxy acylcarnitine molecular species in diabetic myocardium: identification of alterations in mitochondrial fatty acid processing in diabetic myocardium by shotgun lipidomics.

    abstract::Diabetic cardiomyopathy is the result of maladaptive changes in energy homeostasis. However, the biochemical mechanisms underlying dysfunctional lipid metabolism in diabetic myocardium are incompletely understood. Herein, we exploit shotgun lipidomics to demonstrate a 4-fold increase in acylcarnitines in diabetic myoc...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi047773a

    authors: Su X,Han X,Mancuso DJ,Abendschein DR,Gross RW

    更新日期:2005-04-05 00:00:00

  • Evidence for the amphipathic nature and tilted topology of helices 4 and 5 in the closed state of the colicin E1 channel.

    abstract::The membrane-bound closed channel structure of helices 4 and 5 (Lys-406-Asp-446) of the colicin E1 channel domain was investigated by using a site-directed fluorescence labeling technique. A bimane probe was covalently attached to a cysteine residue in a series of single-cysteine mutant proteins to scan each helix in ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi801906v

    authors: Ho D,Merrill AR

    更新日期:2009-02-17 00:00:00

  • Pre-steady-state studies of phosphite dehydrogenase demonstrate that hydride transfer is fully rate limiting.

    abstract::Phosphite dehydrogenase (PTDH) is a unique NAD-dependent enzyme that catalyzes the oxidation of inorganic phosphite to phosphate. The enzyme has great potential for cofactor regeneration, and mechanistic studies have provided some insight into the residues that are important for catalysis. In this investigation, pre-s...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi701550c

    authors: Fogle EJ,van der Donk WA

    更新日期:2007-11-13 00:00:00

  • Structure-based design of an intramolecular proton transfer site in murine carbonic anhydrase V.

    abstract::Carbonic anhydrase V (CA V) is a mitochondrial enzyme that catalyzes the hydration of CO2 to produce bicarbonate and a proton. The catalytic properties of wild-type murine CA V suggest the presence of a proton shuttle residue having pKa = 9.2, the role of which is to transfer a proton from zinc-bound water to solution...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi9608018

    authors: Heck RW,Boriack-Sjodin PA,Qian M,Tu C,Christianson DW,Laipis PJ,Silverman DN

    更新日期:1996-09-10 00:00:00

  • Modulation of the cytochrome P450 reductase redox potential by the phospholipid bilayer.

    abstract::Cytochrome P450 reductase (CPR) is a tethered membrane protein which transfers electrons from NADPH to microsomal P450s. We show that the lipid bilayer has a role in defining the redox potential of the CPR flavin domains. In order to quantitate the electrochemical behavior of this central redox protein, full-length CP...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi9011435

    authors: Das A,Sligar SG

    更新日期:2009-12-29 00:00:00

  • Parameters affecting low-pH-mediated fusion of liposomes with the plasma membrane of cells infected with influenza virus.

    abstract::Unilamellar liposomes can be fused at low pH with the plasma membrane of cells that express the hemagglutinin glycoprotein of influenza virus on their surface [van Meer, G., & Simons, K. (1983) J. Cell Biol. 97, 1365-1374]. Here, we have resolved this fusion process into two kinetically distinct steps. The first and m...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00335a030

    authors: van Meer G,Davoust J,Simons K

    更新日期:1985-07-02 00:00:00

  • Stiffness of Cargo-Motor Linkage Tunes Myosin VI Motility and Response to Load.

    abstract::We examine the effect of cargo-motor linkage stiffness on the mechanobiological properties of the molecular motor myosin VI. We use the programmability of DNA nanostructures to modulate cargo-motor linkage stiffness and combine it with high-precision optical trapping measurements to measure the effect of linkage stiff...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/acs.biochem.9b00422

    authors: Shrivastava R,Rai A,Salapaka M,Sivaramakrishnan S

    更新日期:2019-11-26 00:00:00

  • Interaction of mant-adenosine nucleotides and magnesium with kinesin.

    abstract::Displacement of the fluorescent substrate analogue methylanthraniloyl ADP (mant-ADP) from kinesin by excess ATP results in a biphasic fluorescent transient. The pH and microtubule dependence of the rates and amplitudes indicates that the two phases are produced by release of bound mant-ADP, with an excess of the 3'-is...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi972742j

    authors: Cheng JQ,Jiang W,Hackney DD

    更新日期:1998-04-14 00:00:00

  • Structural and thermodynamic basis of affinity in anti-dinitrophenyl antibody.

    abstract::The thermodynamic quantities of the anti-dinitrophenyl antibody-hapten interaction are reported for rabbit, goat, and guinea pig antibodies. Rabbit and goat antibodies had similar exothermic enthalpy changes for their reaction with 2,4-dinitrophenyl-L-lysine (-13.9 and -14.8 kcal/mol, respectively). The enthalpy chang...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00694a032

    authors: Halsey JF,Cebra JJ,Biltonen RL

    更新日期:1975-11-18 00:00:00

  • Interactions of the antimicrobial peptides temporins with model biomembranes. Comparison of temporins B and L.

    abstract::Temporins are short (10-13 amino acids) and linear antimicrobial peptides first isolated from the skin of the European red frog, Rana temporaria, and are effective against Gram-positive bacteria and Candida albicans. To get insight into their mechanism(s) of action, we compared the effects on model membranes exerted b...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi011929e

    authors: Zhao H,Rinaldi AC,Di Giulio A,Simmaco M,Kinnunen PK

    更新日期:2002-04-02 00:00:00

  • A designed protein with packing between left-handed and right-handed helices.

    abstract::A common motif in protein structures is the assembly of alpha-helices. Natural alpha-helical assemblies, such as helical bundles and coiled coils, consist of multiple right-handed alpha-helices. Here we design a protein complex containing both left-handed and right-handed helices, with peptides of D- and L-amino acids...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi010725v

    authors: Sia SK,Kim PS

    更新日期:2001-07-31 00:00:00

  • Main chain and side chain dynamics of a heme protein: 15N and 2H NMR relaxation studies of R. capsulatus ferrocytochrome c2.

    abstract::A detailed characterization of the main chain and side chain dynamics in R. capsulatus ferrocytochrome c(2) derived from (2)H NMR relaxation of methyl group resonances is presented. (15)N relaxation measurements confirm earlier results indicating that R. capsulatus ferrocytochrome c(2) exhibits minor rotational anisot...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi0102252

    authors: Flynn PF,Bieber Urbauer RJ,Zhang H,Lee AL,Wand AJ

    更新日期:2001-06-05 00:00:00

  • Evolution of enzymatic activity in the enolase superfamily: structure of o-succinylbenzoate synthase from Escherichia coli in complex with Mg2+ and o-succinylbenzoate.

    abstract::The X-ray structures of the ligand free (apo) and the Mg(2+)*o-succinylbenzoate (OSB) product complex of o-succinylbenzoate synthase (OSBS) from Escherichia coli have been solved to 1.65 and 1.77 A resolution, respectively. The structure of apo OSBS was solved by multiple isomorphous replacement in space group P2(1)2(...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi000855o

    authors: Thompson TB,Garrett JB,Taylor EA,Meganathan R,Gerlt JA,Rayment I

    更新日期:2000-09-05 00:00:00

  • Rhodamine 123 binds to multiple sites in the multidrug resistance protein (MRP1).

    abstract::The mechanisms of MRP1-drug binding and transport are not clear. In this study, we have characterized the interaction between MRP1 and rhodamine 123 (Rh123) using the photoreactive-iodinated analogue, [(125)I]iodoaryl azido-rhodamine 123 (or IAARh123). Photoaffinity labeling of plasma membranes from HeLa cells transfe...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi0020574

    authors: Daoud R,Kast C,Gros P,Georges E

    更新日期:2000-12-19 00:00:00

  • Mechanistic Characterization of Escherichia coli l-Aspartate Oxidase from Kinetic Isotope Effects.

    abstract::l-Aspartate oxidase, encoded by the nadB gene, is the first enzyme in the de novo synthesis of NAD+ in bacteria. This FAD-dependent enzyme catalyzes the oxidation of l-aspartate to generate iminoaspartate and reduced flavin. Distinct from most amino acid oxidases, it can use either molecular oxygen or fumarate to reox...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/acs.biochem.7b00307

    authors: Chow C,Hegde S,Blanchard JS

    更新日期:2017-08-08 00:00:00

  • Steady-state kinetics and isotope effects on the mutant catalytic trimer of aspartate transcarbamoylase containing the replacement of histidine 134 by alanine.

    abstract::A detailed kinetic analysis of the catalytic trimer of aspartate transcarbamoylase containing the active site substitution H134A was performed to investigate the role of His 134 in the catalytic mechanism. Replacement of histidine by alanine resulted in decreases in the affinities for the two substrates, carbamoyl pho...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00143a031

    authors: Waldrop GL,Turnbull JL,Parmentier LE,O'Leary MH,Cleland WW,Schachman HK

    更新日期:1992-07-21 00:00:00

  • Regioselectivity in the homolytic cleavage of S-adenosylmethionine.

    abstract::The observed regioselectivity of the homolytic cleavage of S-adenosylmethionine (SAM) by the radical SAM enzymes is modeled by free radical displacement reactions at sulfoxide centers. These displacements are also regioselective, in direct consequence of the reaction mechanism. The selectivity in the radical SAM react...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi101509u

    authors: Kampmeier JA

    更新日期:2010-12-28 00:00:00

  • Gene structure and chromosomal localization of plasma kallikrein.

    abstract::Plasma kallikrein (Fletcher factor) is a hepatic serine proteinase that participated in the early phase of blood coagulation. From two genomic libraries, we succeeded to isolate four overlapping clones representing the entire rat plasma kallikrein gene. Using selective DNA sequencing, polymerase chain reactions, and r...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00220a027

    authors: Beaubien G,Rosinski-Chupin I,Mattei MG,Mbikay M,Chrétien M,Seidah NG

    更新日期:1991-02-12 00:00:00

  • Interaction of an engineered [3Fe-4S] cluster with a menaquinol binding site of Escherichia coli DMSO reductase.

    abstract::We have characterized by EPR the interaction of the Em,7 = -50 mV [4Fe-4S] cluster of Escherichia coli DMSO reductase (DmsABC) with a menaquinol (MQH2) binding site. Potentiometric titrations indicate that in DmsAB(C102S)C, the Em,7 = -50 mV [4Fe-4S] cluster is replaced by an Em,7 = +260 mV [3Fe-4S] cluster. The Q-poo...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi951584y

    authors: Rothery RA,Weiner JH

    更新日期:1996-03-12 00:00:00

  • Pressure effects and thermal stability of myosin rods and rod minifilaments: fluorescence and circular dichroism studies.

    abstract::In the present study hydrostatic pressure was applied upon both skeletal myosin rod molecules and rod minifilaments to learn more of the intra- and intermolecular interaction behavior of myosin. Applied pressure disassembled the rod minifilaments into individual rod molecules and dissociated each myosin rod molecule i...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00184a028

    authors: King L,Liu CC,Lee RF

    更新日期:1994-05-10 00:00:00