Abstract:
:NLRP4 is a member of the nucleotide-binding and leucine-rich repeat receptor (NLR) family of cytosolic receptors and a member of an inflammation signaling cascade. Here, we present the crystal structure of the NLRP4 pyrin domain (PYD) at 2.3 Å resolution. The NLRP4 PYD is a member of the death domain (DD) superfamily and adopts a DD fold consisting of six α-helices tightly packed around a hydrophobic core, with a highly charged surface that is typical of PYDs. Importantly, however, we identified several differences between the NLRP4 PYD crystal structure and other PYD structures that are significant enough to affect NLRP4 function and its interactions with binding partners. Notably, the length of helix α3 and the α2-α3 connecting loop in the NLRP4 PYD are unique among PYDs. The apoptosis-associated speck-like protein containing a CARD (ASC) is an adaptor protein whose interactions with a number of distinct PYDs are believed to be critical for activation of the inflammatory response. Here, we use co-immunoprecipitation, yeast two-hybrid, and nuclear magnetic resonance chemical shift perturbation analysis to demonstrate that, despite being important for activation of the inflammatory response and sharing several similarities with other known ASC-interacting PYDs (i.e., ASC2), NLRP4 does not interact with the adaptor protein ASC. Thus, we propose that the factors governing homotypic PYD interactions are more complex than the currently accepted model, which states that complementary charged surfaces are the main determinants of PYD-PYD interaction specificity.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Eibl C,Grigoriu S,Hessenberger M,Wenger J,Puehringer S,Pinheiro AS,Wagner RN,Proell M,Reed JC,Page R,Diederichs K,Peti Wdoi
10.1021/bi3007059subject
Has Abstractpub_date
2012-09-18 00:00:00pages
7330-41issue
37eissn
0006-2960issn
1520-4995journal_volume
51pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::The properties of a series of new potentiometric membrane probes have been explored. The probes all contain an (aminostyryl)pyridinium chromophore or a more highly conjugated analogue. The spectral properties of the dyes are discussed in terms of the excitation-induced charge shift from the pyridine to the aniline; th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00342a010
更新日期:1985-10-08 00:00:00
abstract::The promoter fragment -1550/+129 of the human hepatic triglyceride lipase (HTGL) gene drives the expression of the CAT gene in HepG2 cells, albeit at very low levels. Transient transfections in HepG2 and HeLa cells of 5' deletion constructs indicated that the regulatory elements that control this expression are locate...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00088a018
更新日期:1993-09-21 00:00:00
abstract::The mechanisms that control N protein dependent antitermination in phage lambda have counterparts in many eukaryotic systems, including specific regulatory interactions of the antitermination protein with the nascent RNA transcript. Here we describe the specific and nonspecific RNA binding modes of antitermination pro...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi961920q
更新日期:1997-02-11 00:00:00
abstract::Replacement of methionine (Met) residues by selenomethionine (SeMet) was recently shown to facilitate the crystallographic analysis of protein structure through the application of multi-wavelength anomalous diffraction techniques [Yang et al. (1990) Science (Washington, D.C.) 249, 1398-1405]. The availability of SeMet...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00110a009
更新日期:1991-11-19 00:00:00
abstract::Methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD) catalyzes interconversion of 5,10-methylene-tetrahydrofolate and 10-formyl-tetrahydrofolate in the one-carbon metabolic pathway. In some organisms, the essential requirement of 10-formyl-tetrahydrofolate may also be fulfilled by formyltetrahydrofolate synth...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b00400
更新日期:2015-06-09 00:00:00
abstract::A panel of 10 hybridomas was assembled to assess the influence of various genetic and biological factors upon glycosylation of secreted monoclonal IgG. After exhaustive Pronase digestion of IgG, glycopeptides were characterized chromatographically by apparent size, charge, and concanavalin A (Con A)-Sepharose and Lens...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00429a065
更新日期:1989-02-07 00:00:00
abstract::The two disparate functions of DCoH1 (dimerization cofactor of HNF-1)/PCD (pterin-4a-carbinolamine dehydratase) are associated with a change in oligomeric state. DCoH dimers enhance the activity of the diabetes-associated transcription factor HNF-1α (hepatocyte nuclear factor-1α), while the PCD activity of DCoH1 homot...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi1015056
更新日期:2010-11-30 00:00:00
abstract::The HIV-1 fusion peptide serves as a useful model system for understanding viral/target cell fusion, at least to the lipid-mixing stage. Previous solid-state NMR studies have shown that the membrane-bound HIV-1 fusion peptide adopts an extended conformation in a lipid mixture close to that of host cells of the virus. ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0348157
更新日期:2003-10-14 00:00:00
abstract::Human fibroblast interferon binds to a concanavalin A-agarose (Con A-Sepharose) equilibrated with methyl alpha-D-mannopyranoside, or levan; in contrast, it is only partially retarded on a similar column equilibrated with ethylene glycol. Interferon does not bind, however, to a lectin column equilibrated with both meth...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00648a039
更新日期:1976-02-10 00:00:00
abstract::In this paper, we report kinetic studies for the chymotryptic activity of the 20S proteasome. Major observations include the following: (1) Reaction progress curves that are recorded at concentrations of Suc-Leu-Leu-Val-Tyr-AMC greater than about 40 microM are biphasic and characterized by initial velocities that deca...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi952262x
更新日期:1996-04-02 00:00:00
abstract::Analysis of [gamma-32P]ATP in vitro labeled nuclear RNA shows transfer of gamma-32P from ATP to form the 5'-terminal monophosphate for large RNA molecules. This finding is an actively transcribing nuclear system capable of guanylation and methylation reactions indicates that polynucleotide kinase activity in the eukar...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00638a016
更新日期:1977-09-20 00:00:00
abstract::Mismatches in DNA occur naturally during replication and as a result of endogenous DNA damaging agents, but the mismatch repair (MMR) pathway acts to correct mismatches before subsequent rounds of replication. Rhodium metalloinsertors bind to DNA mismatches with high affinity and specificity and represent a promising ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi2015822
更新日期:2011-12-20 00:00:00
abstract::Intracellular reduction of carcinogenic Cr(VI) generates Cr-DNA adducts formed through the coordination of Cr(III) to DNA phosphates (phosphotriester-type adduct). Here, we examined the role of Cr(III)-DNA adducts in mutagenesis induced by metabolism of Cr(VI) with cysteine. Reduction of Cr(VI) caused a strong oxidati...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0015459
更新日期:2001-01-16 00:00:00
abstract::Escherichia coli DNA photolyase contains 1,5-dihydro-FAD (FADH2) plus 5,10-methenyl-tetrahydrofolate (5,10-CH+-H4folate). Both chromophores are fluorescent, and either can function as a sensitizer in catalysis. At 77 K separate fluorescence emission bands are observed for FADH2 (lambda max = 505 nm, shoulder at 540 nm...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00425a007
更新日期:1988-12-13 00:00:00
abstract::NMR spectroscopic experiments were performed to study the structure of synthetic peptides identical to two extracellular regions of the human thrombin receptor. The smaller molecule, comprising 14 amino acids, was biologically active and was equivalent to the "tethered ligand" exposed after cleavage of the receptor by...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00186a005
更新日期:1994-05-24 00:00:00
abstract::The effects of incubation of erythrocyte ghosts under various conditions (ionic strength or addition of ankyrin, diamines, or ATP) on the lateral motion of band 3 in the membranes were studied by using the fluorescence photobleaching recovery technique. Incubation of ghosts with exogenous ankyrin increased the immobil...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00368a045
更新日期:1986-10-07 00:00:00
abstract::R67 dihydrofolate reductase (DHFR) is an R-plasmid encoded enzyme that confers resistance to the antibacterial drug trimethoprim. This enzyme is not homologous in sequence or structure to chromosomal DHFRs. Equilibrium folding of tetrameric R67 DHFR was studied and found to be fully reversible. Formation of an inactiv...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00180a018
更新日期:1994-04-12 00:00:00
abstract::Murine class alpha glutathione S-transferase subunit types A2 (mGSTA2-2) and A1 (mGSTA1-1) have high catalytic efficiency for glutathione (GSH) conjugation of the ultimate carcinogenic metabolite of benzo[a]pyrene, (+)-anti-7,8-dihydroxy-9,10-oxy-7,8,9,10-tetrahydrobenzo[a]pyrene, [(+)-anti-BPDE]. Only 10 residues dif...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi026778+
更新日期:2003-02-04 00:00:00
abstract::The heteronemertine worm Cerebratulus lacteus produces a family of three structurally homologous proteins that function as direct lytic factors for a variety of cells [Kem, W. R., & Blumenthal, K. M. (1978) J. Biol. Chem. 253, 5752-5757]. It is demonstrated herein that the hemolytic activity of the most abundant varia...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00261a007
更新日期:1982-08-31 00:00:00
abstract::The first use of computer-simulation studies to examine heparin's structure has been reported. The product distributions obtained when porcine mucosal heparins were depolymerized with heparinase have been compared to computer-simulated distributions. The modeled distribution was relatively unaffected by the polydisper...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00347a045
更新日期:1985-12-17 00:00:00
abstract::Porcine aortic myosin is a smooth muscle contractile protein similar to its striated muscle counterpart. Electrophoresis in sodium dodecyl sulfate indicates that the molecule consists of three classes of subunits with polypeptide chain molecular weights of 192,000, 19,000, and 15,000. At 277 nm the absorption spectrum...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00576a003
更新日期:1979-05-01 00:00:00
abstract::Long-range intraprotein interactions give rise to many important protein behaviors. Understanding how energy is transduced through protein structures to either transmit a signal or elicit conformational changes is therefore a current challenge in structural biology. In an effort to understand such linkages, multiple V...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi060652l
更新日期:2006-06-27 00:00:00
abstract::The structural domains of human apolipoprotein(a) (apo(a)) in the lipoprotein(a) (Lp(a)) particle have been recently investigated by limited proteolysis [Huby, T., Doucet, C., Dieplinger, H., Chapman, J., & Thillet, J. (1994) Biochemistry 33, 3335-3341]. We have shown that apo(a) can be cleaved into two structural dom...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00022a011
更新日期:1995-06-06 00:00:00
abstract::We have previously shown that the anticodon of methionine tRNAs contains most, if not all, of the nucleotides required for specific recognition of tRNA substrates by Escherichia coli methionyl-tRNA synthetase [Schulman, L. H., & Pelka, H. (1988) Science 242, 765-768]. Previous cross-linking experiments have also ident...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00461a003
更新日期:1990-03-06 00:00:00
abstract::Safe and effective algaecides are needed to control agriculturally and environmentally significant algal species. Four series (6, 10, 17, and 21) of 29 novel 4-aminopyrimidine derivatives were rationally designed and synthesized. A part of 10, 17, and 21 displayed potent inhibition of Escherichia coli pyruvate dehydro...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00636
更新日期:2017-12-12 00:00:00
abstract::The individual amide proton exchange rates in Tendamistat at pH 3.0 and 50 degrees C were measured by using two-dimensional 1H nuclear magnetic resonance. Overall, it was found that the distribution of exchange rates along the sequence is dominated by the interstrand hydrogen bonds of the beta-sheet structures. The sl...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00394a030
更新日期:1987-10-06 00:00:00
abstract::Formylglycinamide ribonucleotide amidotransferase (FGAR-AT) catalyzes the ATP- and glutamine-dependent formation of formylglycinamidine ribonucleotide, ADP, P(i), and glutamate in the fourth step of de novo purine biosynthesis. Like all amidotransferases (ATs), FGAR-AT is proposed to channel ammonia between a glutamin...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi049127h
更新日期:2004-08-17 00:00:00
abstract::The innate immune response to infection or injury involves an antigen-antibody triggered classical pathway (CP) of complement activation, in which soluble and cell surface plasma proteins cooperatively effect elimination of foreign organisms and damaged host cells. However, protracted or dysfunctional complement activ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi900679r
更新日期:2009-09-08 00:00:00
abstract::A reinvestigation of the metal ion activation and specificity of CTP synthetase was begun in order to separate effects due to binding of free metal ions and binding of nucleotide-complexed ions. Apo-CTP synthetase was prepared by dialysis against 5 mM EDTA. Analysis of apo-enzyme by atomic absorption spectroscopy reve...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00065a032
更新日期:1993-04-13 00:00:00
abstract::The hydration of CO2 catalyzed by carbonic anhydrase requires proton transfer from the zinc-bound water at the active site to solution for each cycle of catalysis. In the most efficient of the mammalian carbonic anhydrases, isozyme II, this transfer is facilitated by a proton shuttle residue, His 64. Murine carbonic a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9729209
更新日期:1998-05-19 00:00:00