Amide proton exchange in the alpha-amylase polypeptide inhibitor Tendamistat studied by two-dimensional 1H nuclear magnetic resonance.

abstract：:Antifreeze proteins (AFPs) inhibit the growth of ice by binding to the surface of ice crystals, preventing the addition of water molecules to cause a local depression of the freezing point. AFPs from insects are much more effective at depressing the freezing point than fish AFPs. Here, we have investigated the possibi...

journal_title：Biochemistry

authors： Marshall CB,Tomczak MM,Gauthier SY,Kuiper MJ,Lankin C,Walker VK,Davies PL

更新日期：2004-01-13 00:00:00

abstract：:The Gbeta and Ggamma subunit of the heterotrimeric G proteins form a functional dimer that is stable once assembled in vivo or in vitro. The requirements, mechanism, and specificity of dimer formation are still incompletely understood, but represent important biochemical processes involved in the specificity of cellul...

journal_title：Biochemistry

authors： Dingus J,Wells CA,Campbell L,Cleator JH,Robinson K,Hildebrandt JD

更新日期：2005-09-06 00:00:00

abstract：:We measured the time course of skeletal muscle actin polymerization at different actin concentrations. In 0.1 M KCl with 1 mM Mg2+, log/log plots of the rate of the early, slow phase of polymerization vs. actin concentration were linear with slopes from 1.0 to 1.3. Computer-assisted calculations of similar curves from...

journal_title：Biochemistry

authors： Cooper JA,Buhle EL Jr,Walker SB,Tsong TY,Pollard TD

更新日期：1983-04-26 00:00:00

abstract：:Binding mechanisms of ADPR-transferase to restricted double-stranded DNA fragments of SV40 and pBR322 DNA were determined by nuclease protection techniques. Top and bottom strands of double-stranded DNA were identified by specific labeling with 32P. Protection against specific exonucleases identified binding of ADPR-t...

journal_title：Biochemistry

authors： Sastry SS,Buki KG,Kun E

更新日期：1989-06-27 00:00:00

abstract：:We previously showed that beta-amyloid precursor protein (APP) is cleaved not only in the middle of the membrane (gamma-cleavage) but also at novel cleavage sites close to the membrane/cytoplasmic boundary (epsilon-cleavage), releasing APP intracellular domains (AICDs) 49-99 and 50-99. To learn more about the relation...

journal_title：Biochemistry

authors： Funamoto S,Morishima-Kawashima M,Tanimura Y,Hirotani N,Saido TC,Ihara Y

更新日期：2004-10-26 00:00:00

abstract：:A subgroup of enzymes in the NAD(P)H:FMN reductase family is comprised of flavin reductases from two-component monooxygenase systems. The diverging structural feature in these FMN reductases is a π-helix centrally located at the tetramer interface that is generated by the insertion of an amino acid in a conserved α4 h...

journal_title：Biochemistry

authors： Musila JM,L Forbes D,Ellis HR

更新日期：2018-07-31 00:00:00

abstract：:The ATP hydrolysis and protein-binding and release cycle of the molecular chaperone DnaK is regulated by the accessory proteins GrpE and DnaJ. Here we describe a study of the formation of complexes between the molecular chaperone DnaK, its nucleotide exchange factor GrpE, and the fluorescent ADP analog N8-[4-[(N'-meth...

journal_title：Biochemistry

authors： Packschies L,Theyssen H,Buchberger A,Bukau B,Goody RS,Reinstein J

更新日期：1997-03-25 00:00:00

abstract：:The translocation (T) domain of diphtheria toxin plays a critical role in moving the catalytic domain across the endosomal membrane. Translocation/insertion is triggered by a decrease in pH in the endosome where conformational changes of T domain occur through several kinetic intermediates to yield a final trans-membr...

journal_title：Biochemistry

authors： Li J,Rodnin MV,Ladokhin AS,Gross ML

更新日期：2014-11-04 00:00:00

abstract：:LL-23 is a natural peptide corresponding to the 23 N-terminal amino acid residues of human host defense cathelicidin LL-37. LL-23 demonstrated, compared to LL-37, a conserved ability to induce the chemokine MCP-1 in human peripheral blood mononuclear cells, a lack of ability to suppress induction of the pro-inflammato...

journal_title：Biochemistry

authors： Wang G,Elliott M,Cogen AL,Ezell EL,Gallo RL,Hancock RE

更新日期：2012-01-17 00:00:00

abstract：:In the present study hydrostatic pressure was applied upon both skeletal myosin rod molecules and rod minifilaments to learn more of the intra- and intermolecular interaction behavior of myosin. Applied pressure disassembled the rod minifilaments into individual rod molecules and dissociated each myosin rod molecule i...

journal_title：Biochemistry

authors： King L,Liu CC,Lee RF

更新日期：1994-05-10 00:00:00

abstract：:Binding of urokinase-type plasminogen activator (uPA) to its cellular receptor (uPAR) renders the cell surface a favored site for plasminogen activation. Recently, a 15-mer peptide antagonist of the uPA-uPAR interaction, with an IC50 value of 10 nM, was identified using phage display technology [Goodson, R. J., Doyle,...

journal_title：Biochemistry

authors： Ploug M,Ostergaard S,Hansen LB,Holm A,Danø K

更新日期：1998-03-17 00:00:00

abstract：:We investigated the effects of substituting two of the four tryptophans (the "inner pair" Trp(9) and Trp(11) or the "outer pair" Trp(13) and Trp(15)) in gramicidin A (gA) channels. The conformational preferences of the doubly substituted gA analogues were assessed using circular dichroism spectroscopy and size-exclusi...

journal_title：Biochemistry

authors： Gu H,Lum K,Kim JH,Greathouse DV,Andersen OS,Koeppe RE 2nd

更新日期：2011-06-07 00:00:00

abstract：:This paper reports on NMR-molecular mechanics structural studies of the (-)-trans-anti-[MC]dG adduct positioned opposite dC in the sequence context of the d(C1-C2-A3-T4-C5-[MC]G6-C7-T8-A9-C10-C11).d(G12-G13-T14++ +-A15-G16-C17-G18- A19-T20-G21-G22) duplex [designated (-)-trans-anti-[MC]dG.dC 11-mer duplex]. This adduc...

journal_title：Biochemistry

authors： Cosman M,Xu R,Hingerty BE,Amin S,Harvey RG,Geacintov NE,Broyde S,Patel DJ

更新日期：1995-05-09 00:00:00

abstract：:Deuterium nuclear magnetic resonance spectra of dimyristoylphosphatidylcholines specifically labeled in positions 2', 3', 4', 6', 8', 10', 12', and 14', of the 2 chain, of an N-deuteriomethylphosphatidylcholine, and of cholesterol-3alpha-d1, have been obtained by the Fourier transform method at 5.46 and 3.52 T on two ...

journal_title：Biochemistry

authors： Oldfield E,Meadows M,Rice D,Jacobs R

更新日期：1978-07-11 00:00:00

abstract：:The extent of nativeness of the stable conformation of the thermolysin fragment containing the carboxyl-terminal third of the protein (from residues 206 to 316, denoted fragment FII) was examined by its immunogenic and antigenic characteristics. Antisera elicited in rabbits by either intact thermolysin or fragment FII...

journal_title：Biochemistry

authors： Vita C,Fontana A,Chaiken IM

更新日期：1982-04-27 00:00:00

abstract：:Glucocorticoid (GC) hormones induce apoptosis in T-cell and pre-B-cell acute lymphoblastic leukemia (ALL) cells. Steroid-mediated apoptosis requires a threshold level of the glucocorticoid receptor (GR) protein, and increasing the intracellular GR levels in ALL cells would augment their hormone sensitivity. A protein ...

journal_title：Biochemistry

authors： Geng CD,Vedeckis WV

更新日期：2010-10-19 00:00:00

abstract：:We studied the binding of peptides containing five basic residues to membranes containing acidic lipids. The peptides have five arginine or lysine residues and zero, one, or two alanines between the basic groups. The vesicles were formed from mixtures of a zwitterionic lipid, phosphatidylcholine, and an acidic lipid, ...

journal_title：Biochemistry

authors： Mosior M,McLaughlin S

更新日期：1992-02-18 00:00:00

abstract：:Three hybridomas secreting monoclonal antibodies, HH1, HH2, and HH3, defining different difucosyl A structures (ALeb or ALey), have been established. Antibody HH1 (IgG2a) reacts specifically with the difucosyl A structure irrespective of a type 1 or type 2 chain, while antibody HH2 (IgG3) reacts exclusively with the d...

journal_title：Biochemistry

authors： Clausen H,McKibbin JM,Hakomori S

更新日期：1985-10-22 00:00:00

abstract：:Self-assembly properties of HIV-1 integrase were investigated by time-resolved fluorescence anisotropy using tryptophanyl residues as a probe. From simulation analyses, we show that suitable photon counting leads to an accurate determination of long rotational correlation times in the range of 20-80 ns, permitting the...

journal_title：Biochemistry

authors： Deprez E,Tauc P,Leh H,Mouscadet JF,Auclair C,Brochon JC

更新日期：2000-08-08 00:00:00

abstract：:Actin, an abundant cytosolic protein in eukaryotic cells, is dependent on the interaction with the chaperonin tail-less complex polypeptide 1 ring complex (TRiC) to fold to the native state. The prokaryotic chaperonin GroEL also binds non-native beta-actin, but is unable to guide beta-actin toward the native state. In...

journal_title：Biochemistry

authors： Villebeck L,Moparthi SB,Lindgren M,Hammarström P,Jonsson BH

更新日期：2007-11-06 00:00:00

abstract：:High-resolution proton nuclear magnetic resonance (1H NMR) measurements were made on myosin, heavy meromyosin (HMM), myosin subfragment 1 (S1), light meromyosin (LMM), and actin. A strong signal from amino acid side chains undergoing motions too fast to be accounted for by simple rotations of groups on a rigid backbon...

journal_title：Biochemistry

authors： Highsmith S,Akasaka K,Konrad M,Goody R,Holmes K,Wade-Jardetzky N,Jardetzky O

更新日期：1979-09-18 00:00:00

abstract：:Quasi-elastic laser light scattering is used to examine the cholera toxin mediated agglutination of ganglioside GM1 containing phospholipid vesicles and Gm1-coated polystyrene spheres. We find that the ability of cholera toxin to agglutinate Gm1-containing phospholipid vesicles depends markedly on the lipid compositio...

journal_title：Biochemistry

authors： Dwyer JD,Bloomfield VA

更新日期：1982-06-22 00:00:00

abstract：:Replacement of methionine (Met) residues by selenomethionine (SeMet) was recently shown to facilitate the crystallographic analysis of protein structure through the application of multi-wavelength anomalous diffraction techniques [Yang et al. (1990) Science (Washington, D.C.) 249, 1398-1405]. The availability of SeMet...

journal_title：Biochemistry

authors： Boles JO,Cisneros RJ,Weir MS,Odom JD,Villafranca JE,Dunlap RB

更新日期：1991-11-19 00:00:00

abstract：:It is now well established that guanine nucleotides are allosteric effectors of the vinca alkaloid-induced self-association of tubulin. GDP enhances self-association for vinblastine-, vincristine- and vinorelbine-induced spiral assembly relative to GTP by 0.90 +/- 0.17 kcal/mol [Lobert et al. (1996) Biochemistry 35, 6...

journal_title：Biochemistry

authors： Vulevic B,Lobert S,Correia JJ

更新日期：1997-10-21 00:00:00

abstract：:Members of the phospholipase C-beta (PLC-beta) family of proteins are activated either by G alpha or G beta gamma subunits of heterotrimeric G proteins. To define specific regions of PLC-beta 3 that are involved in binding and activation by G beta gamma, a series of fragments of PLC-beta 3 as glutathione-S-transferase...

journal_title：Biochemistry

authors： Barr AJ,Ali H,Haribabu B,Snyderman R,Smrcka AV

更新日期：2000-02-22 00:00:00

abstract：:Primary kinetic isotope effects on Vmax were observed in both reaction directions upon racemizing samples of [2-2H]glutamate with the cofactor-independent glutamate racemase from Lactobacillus. This supports a deprotonation/protonation mechanism for racemization in which the breaking of the carbon-hydrogen bond at C-2...

journal_title：Biochemistry

authors： Tanner ME,Gallo KA,Knowles JR

更新日期：1993-04-20 00:00:00

abstract：:The structure of the specific phosphate binding loop (P-loop) of bovine protein tyrosine phosphatase (BPTP) is very similar to that present in high M(r) PTPases. Site-directed mutagenesis was used to explore the role of several conserved residues involved in forming the P-loop of BPTP. Thus, Ser-19 and Ser-43 were ind...

journal_title：Biochemistry

authors： Evans B,Tishmack PA,Pokalsky C,Zhang M,Van Etten RL

更新日期：1996-10-22 00:00:00

abstract：:The present study was undertaken for the purpose of evaluating the solvent perturbation technique as a method to identify enzyme catalytic residues. For establishment of expected directions and sizes of pKa perturbations for different types of acids in different classes of solvents, a study of the pKa of a series of a...

journal_title：Biochemistry

authors： Grace S,Dunaway-Mariano D

更新日期：1983-08-30 00:00:00

abstract：:Treatment of soybean lipoxygenase isozyme 1 with its substrates, linoleic acid and oxygen, or product, 13(S)-hydroperoxy-9,11(Z,E)-octadecadienoic acid (13-HPOD), leads to the appearance of a purple color. Although the structure of the chromophore has not been determined, we present strong evidence that it is an Fe(3+...

journal_title：Biochemistry

authors： Nelson MJ,Chase DB,Seitz SP

更新日期：1995-05-09 00:00:00

abstract：:The disulfide bond location of a homodimeric peptide, prothoracicotropic hormone (PTTH) of the silkworm, Bombyx mori, was determined by a combination of partial reduction and sequence analysis of peptide fragments generated through a partial reduction of PTTH followed by alkylation and enzyme digestion. The partial re...

journal_title：Biochemistry

authors： Ishibashi J,Kataoka H,Isogai A,Kawakami A,Saegusa H,Yagi Y,Mizoguchi A,Ishizaki H,Suzuki A

更新日期：1994-05-17 00:00:00