Abstract：
:Effects of human insulin on glucose metabolism in the yeast Saccharomyces cerevisiae were studied in this report. Under two conditions of growth limitation (glucose-grown cells during transition to stationary phase or spheroplasts during incubation in synthetic glucose medium), human insulin (10 and 1 microM, respectively) enhanced glycogen accumulation and glycogen synthase activity by 40-60% compared to control cells. Glycogen phosphorylase activity was also increased under the same conditions, but this stimulation was diminished by 35-45% in insulin-treated compared to control cells. Thus, under growth limitation, insulin causes glycogen phosphorylase and glycogen synthase to become more sensitive to inactivation and activation, respectively. In glucose-induced spheroplasts, insulin (1 microM), in addition to glycogen accumulation, led to about 2-fold increases of the rates of ethanol production and glucose oxidation compared to control cells, and the maximal concentration of hexose 6-phosphate was increased by 30-40%. In contrast, glucose transport as well as the levels of the allosteric regulators, fructose 2,6-bisphosphate and cAMP, were not altered at all. Snf1 kinase is assumed to be involved in the regulation of glycogen metabolism in yeast, although it does not seem to be modulated directly by the glucose concentration. Snf1 kinase activity was elevated 5-10-fold in response to insulin both during glucose induction of yeast spheroplasts and during transition to stationary phase of glucose-grown cells. We conclude that Saccharomyces cerevisiae and insulin-sensitive mammalian cells share some parts of the signaling cascades regulating oxidative and nonoxidative glucose metabolism in response to glucose and insulin.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Müller G,Rouveyre N,Crecelius A,Bandlow Wdoi
10.1021/bi972071psubject
Has Abstractpub_date
1998-06-16 00:00:00pages
8683-95issue
24eissn
0006-2960issn
1520-4995pii
bi972071pjournal_volume
37pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Ribonucleotide reductase from Escherichia coli consists of two nonidentical subunits, named protein B1 (170 000) and protein B2 (87 000). We purified and characterized five monoclonal antibodies against B1 and three against B2 from hybridomas obtained by fusion of spleen cells from immunized mice and the myeloma cell ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00352a018
更新日期：1986-02-25 00:00:00
abstract：:The plasma membrane serotonin transporter (SERT) has an important role in terminating serotonergic neurotransmission by re-uptake of 5-HT from the synaptic cleft. The expression of SERT on the cell surface is therefore a critical factor. In this study, we examined the role of the carboxyl terminus of SERT in trafficki...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0508688
更新日期：2006-01-31 00:00:00
abstract：:Peptidylglycine alpha-hydroxylating monooxygenase (PHM) is a copper, ascorbate, and molecular oxygen dependent enzyme that catalyzes the first step leading to the C-terminal amidation of glycine-extended peptides. The catalytic core of PHM (PHMcc), refined to residues 42-356 of the PHM protein, was expressed at high l...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9708747
更新日期：1997-09-09 00:00:00
abstract：:The solution behavior of bilirubin ditaurate (BDT), the first naturally occurring conjugated bile pigment to be physically and chemically characterized, was assessed in aqueous solution and in monomeric and micellar solutions of common taurine-conjugated bile salts (BS). Analytical ultracentrifugation revealed that BD...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi501251v
更新日期：2015-02-24 00:00:00
abstract：:The complete amino acid sequence of the major component myoglobin from Amazon River dolphin, Inia geoffrensis, was determined by specific cleavage of the protein to obtain large peptides which are readily degraded by the automatic sequencer. Three easily separable peptides were obtained by cleaving the protein with cy...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00695a018
更新日期：1975-12-02 00:00:00
abstract：:Native calf thymus DNA was reacted with N-acetoxy-N-2-acetylaminofluorene (N-AcO-AAF) and its 7-fluoro and 7-iodo derivatives. Different ways of purification of the fluorene modified DNA samples were checked in order ot obtain a nucleic acid free from all noncovalently bound fluorene residues. The decrease in melting ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00660a027
更新日期：1976-07-27 00:00:00
abstract：:Potent inhibitors of human cysteine proteases of the papain family have been made and assayed versus a number of relevant family members. We describe the synthesis of peptide alpha-ketoheterocyclic inhibitors that occupy binding subsites S1'-S3 of the cysteine protease substrate recognition cleft and that form a rever...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi035041x
更新日期：2003-12-30 00:00:00
abstract：:The calcitonin gene-related peptide (CGRP) receptor is a heterodimer of two membrane proteins: calcitonin receptor-like receptor (CLR) and receptor activity-modifying protein 1 (RAMP1). CLR is a class B G-protein-coupled receptor (GPCR), possessing a characteristic large amino-terminal extracellular domain (ECD) impor...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi901848m
更新日期：2010-03-09 00:00:00
abstract：:Myosin cross-bridge subfragment 1 (S1) is the ATP catalyzing motor protein in muscle. It consists of three domains that catalyze ATP and bind actin (catalytic), conduct energy transduction (converter), and transport the load (lever arm). Force development during contraction is thought to result from rotary lever arm m...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi002387o
更新日期：2001-04-17 00:00:00
abstract：:The regulation of the rate-controlling enzyme in cholesterol biosynthesis and of the incorporation of [14C]oleate into cholesterol esters were studied in established lymphoid cell lines from normal subjects and compared with that of eight patients with genetic abnormalities of lipid metabolism. The activity of 3-hydro...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00648a011
更新日期：1976-02-10 00:00:00
abstract：:Lysyl-tRNA synthetase is a member of the class II aminoacyl-tRNA synthetases and catalyses the specific aminoacylation of tRNA(Lys). The crystal structure of the constitutive lysyl-tRNA synthetase (LysS) from Escherichia coli has been determined to 2.7 A resolution in the unliganded form and in a complex with the lysi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi001487r
更新日期：2000-10-24 00:00:00
abstract：:To study the role of the N-terminal region of the estrogen receptor (ER) in transcription activation and in DNA binding, we constructed a mutant of the Xenopus laevis ER which lacks amino acids 1-159 (XER160/586). In transient transfections, XER160/586 exhibited < 10% of the activity of wild-type XER on a synthetic pr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00012a013
更新日期：1995-03-28 00:00:00
abstract：:We have determined the mechanism of DNA-dependent protein kinase (DNA-PK) inhibition by cis-diamminedichloroplatinum(II)-(cisplatin-) damaged DNA. We previously have demonstrated that Ku, the DNA binding subunit of DNA-PK, is capable of binding to DNA duplexes globally damaged with cisplatin but was unable to stimulat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi963124q
更新日期：1997-06-17 00:00:00
abstract：:The gene 5 protein (g5p) encoded by filamentous Ff phages is an ssDNA-binding protein, which binds to and sequesters the nascent ssDNA phage genome in the process of phage morphogenesis. The g5p also binds with high affinity to DNA and RNA sequences that form G-quadruplex structures. However, sequences that would form...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi030177g
更新日期：2004-03-09 00:00:00
abstract：:An IscA homologue within the nif regulon of Azotobacter vinelandii, designated (Nif)IscA, was expressed in Escherichia coli and purified to homogeneity. Purified (Nif)IscA was found to be a homodimer of 11-kDa subunits that contained no metal centers or other prosthetic groups in its as-isolated form. Possible roles f...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi015656z
更新日期：2001-11-20 00:00:00
abstract：:G-Quadruplexes occupy important regulatory regions in the genome. DNA G-quadruplexes in the promoter regions and RNA quadruplexes in the UTRs (untranslated regions) have been individually studied and variously implicated at different regulatory levels of gene expression. However, the formation of G-quadruplexes in the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi401451q
更新日期：2014-06-17 00:00:00
abstract：:In the HLH-LZ protein family, the helix-loop-helix DNA-binding dimerization domain is followed in the sequence by a leucine zipper motif. The precise function of this second dimerization domain is still unclear, since the HLH motif of a subset of this family has been shown to be necessary and sufficient for dimerizati...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00203a027
更新日期：1994-09-20 00:00:00
abstract：:Twelve site-directed mutants of rat cytochrome P450 2B1 distributed over seven positions and four putative substrate recognition sites (SRS) were constructed and expressed in COS cells. Function was examined using androstenedione and testosterone as substrates. Substitutions at positions 303, 360, and 473 did not mark...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00180a040
更新日期：1994-04-12 00:00:00
abstract：:Endogenous DNA damage occurs at a rate of at least 20,000 lesions per cell per day. Base excision repair (BER) is a key pathway for maintaining genome stability. Several pol beta variants were identified as conferring resistance to 3'-azido-3'-deoxythymidine (AZT) in Escherichia coli (Kosa et al. (1999) J. Biol. Chem....
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi702104y
更新日期：2008-08-05 00:00:00
abstract：:The removal of damaged bases by DNA glycosylases is thought to be effectively irreversible, because of an overall equilibrium that favors hydrolysis over synthesis of the N-glycosyl bond. Surprisingly, human alkyladenine DNA glycosylase (AAG) can make damaged DNA by catalyzing formation of an N-glycosyl bond between 1...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi101380d
更新日期：2010-10-26 00:00:00
abstract：:As part of a search for organic compounds that selectively target RNA, we found that specific diphenylfuran derivatives, which are related to compounds that bind to the DNA minor groove, bind very strongly to RNA in a manner very sensitive to the structure of the compounds. In extended development of the diphenylfuran...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi002338b
更新日期：2001-02-06 00:00:00
abstract：:RNA binding proteins play an important role in regulating alternative pre-mRNA splicing and in turn cellular gene expression. Many of these RNA binding proteins occur as gene families with members sharing a high degree of primary structure identity and domain organization yet have tissue-specific expression patterns a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b00256
更新日期：2018-07-03 00:00:00
abstract：:Although tryptophan synthase catalyzes a number of pyridoxal phosphate dependent beta-elimination and beta-replacement reactions that are also catalyzed by tryptophanase, a principal and puzzling difference between the two enzymes lies in the apparent inability of tryptophan synthase to catalyze beta-elimination of in...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00363a010
更新日期：1986-07-29 00:00:00
abstract：:While the insertion of a hydrophobic peptide or membrane protein segment into the bilayer can be spontaneous and driven mainly by the hydrophobic effect, anionic lipids, which comprise ca. 20% of biological membranes, provide a source of electrostatic attractions for binding of proteins/peptides into membranes. To unr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi970030n
更新日期：1997-05-06 00:00:00
abstract：:The c-Jun N-terminal kinases (JNKs) play a wide variety of roles in cellular signaling processes, dictating important, and even divergent, cellular fates. These essential kinases possess docking surfaces distal to their active sites that interact with diverse binding partners, including upstream activators, downstream...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b00776
更新日期：2018-10-09 00:00:00
abstract：:Numerous zinc ectoenzymes are folded and activated in the compartments of the early secretory pathway, such as the ER and the Golgi apparatus, before reaching their final destination. During this process, zinc must be incorporated into the active site; therefore, metalation of the nascent protein is indispensable for ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.9b00924
更新日期：2020-01-14 00:00:00
abstract：:Conformational dynamics are thought to be a prerequisite for the catalytic activity of enzymes. However, the exact relationship between structural fluctuations and function is not well understood. In this work hydrogen/deuterium exchange (HDX) and electrospray ionization mass spectrometry (ESI-MS) are used for explori...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi800463q
更新日期：2008-06-17 00:00:00
abstract：:Phosphotriesterase, as isolated from Pseudomonas diminuta, is capable of detoxifying widely used pesticides such as paraoxon and parathion and various mammalian acetylcholinesterase inhibitors. The enzyme requires a binuclear metal center for activity. Recently, the three-dimensional structure of the apoenzyme was sol...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00025a002
更新日期：1995-06-27 00:00:00
abstract：:We have investigated the microsecond rotational motions of the Ca-ATPase in rabbit skeletal sarcoplasmic reticulum (SR), by measuring the time-resolved phosphorescence anisotropy of erythrosin 5-isothiocyanate (ERITC) covalently and specifically attached to the enzyme. Over a wide range of solvent conditions and tempe...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00468a017
更新日期：1990-04-24 00:00:00
abstract：:Biotin synthase, a member of the "radical SAM" family, catalyzes the final step of the biotin biosynthetic pathway, namely, the insertion of a sulfur atom into dethiobiotin (DTB). The active form of the enzyme contains two iron-sulfur clusters, a [4Fe-4S](2+) cluster liganded by Cys-53, Cys-57, and Cys-60 and the S-ad...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi060662m
更新日期：2006-10-10 00:00:00
