Peptide ketobenzoxazole inhibitors bound to cathepsin K.

abstract：:We have measured T1 and T2 of protein and water protons in hemoglobin solutions using broad-line pulse techniques; selective excitation and detection methods enabled the intrinsic protein and water relaxation rates, as well as the spin-transfer rate between them, to be obtained at 5, 10, and 20 MHz. Water and protein ...

journal_title：Biochemistry

authors： Eisenstadt M

更新日期：1985-07-02 00:00:00

abstract：:Classical molecular dynamics simulations were utilized to investigate the structural and dynamical properties of water in the active site of ketosteroid isomerase (KSI) to provide insight into the role of these water molecules in the enzyme-catalyzed reaction. This reaction is thought to proceed via a dienolate interm...

journal_title：Biochemistry

authors： Hanoian P,Hammes-Schiffer S

更新日期：2011-08-09 00:00:00

abstract：:The complete amino acid sequence of the 4Fe-4S ferredoxin from the thermophilic bacterium Clostridium thermoaceticum has been determined. The protein is extremely thermostable and is the only known clostridial ferredoxin to contain a single [4Fe-4S] cluster. The sequence totals 63 residues and includes the first trypt...

journal_title：Biochemistry

authors： Elliott JI,Yang SS,Ljungdahl LG,Travis J,Reilly CF

更新日期：1982-07-06 00:00:00

abstract：:Phospholamban (PLB) is a major target of the beta-adrenergic cascade in the heart, and functions to modulate rate-limiting conformational transitions involving the transport activity of the Ca-ATPase. To investigate structural changes within the Ca-ATPase that result from the phosphorylation of PLB by cAMP-dependent p...

journal_title：Biochemistry

authors： Negash S,Huang S,Squier TC

更新日期：1999-06-22 00:00:00

abstract：:Site-directed mutagenesis and high-resolution two-dimensional (2D) proton nuclear magnetic resonance (NMR) were used to probe the structural and functional roles of a highly conserved residue, Asp-49, in the interfacial catalysis by bovine pancreatic phospholipase A2 (PLA2, overexpressed in Escherichia coli). Accordin...

journal_title：Biochemistry

authors： Li Y,Yu BZ,Zhu H,Jain MK,Tsai MD

更新日期：1994-12-13 00:00:00

abstract：:Genomic clones containing the human Zn-alpha 2-glycoprotein gene were isolated. Two of them were for the functional gene overlapped, and the other four were for two different pseudogenes (1 and 2) retaining exon-intron organization. The complete DNA sequence of the functional gene (9.3 kb) and its 5'- and 3'-flanking ...

journal_title：Biochemistry

authors： Ueyama H,Deng HX,Ohkubo I

更新日期：1993-12-07 00:00:00

abstract：:The metalloenzyme Cu/Zn-superoxide dismutase (SOD1) catalyzes the reduction of superoxide anions into molecular oxygen and hydrogen peroxide. Hydrogen peroxide can oxidize SOD1, resulting in aberrant protein conformational changes, disruption of SOD1 function, and DNA damage. Cells may have evolved mechanisms of regul...

journal_title：Biochemistry

authors： Auclair JR,Brodkin HR,D'Aquino JA,Petsko GA,Ringe D,Agar JN

更新日期：2013-09-10 00:00:00

abstract：:Human protein Z is a vitamin K-dependent plasma glycoprotein, deficiency of which leads to a mild bleeding tendency. Protein Z appears to assist hemostasis by binding thrombin and promoting its association with phospholipid vesicles. In this study, to characterize the gene for protein Z, its organization and structure...

journal_title：Biochemistry

authors： Fujimaki K,Yamazaki T,Taniwaki M,Ichinose A

更新日期：1998-05-12 00:00:00

abstract：:Ubiquitin was modified with the anionic, arginine-specific reagent 4-(oxoacetyl)phenoxyacetic acid in order to study the relationship between structure and function of the molecule. Four different derivatives (A, B, C, and D) were purified from the reaction mixture by anion-exchange high-performance liquid chromatogra...

journal_title：Biochemistry

authors： Duerksen-Hughes PJ,Xu XX,Wilkinson KD

更新日期：1987-11-03 00:00:00

abstract：:Type II polyketides are a class of natural products that include pharmaceutically important aromatic compounds such as the antibiotic tetracycline and antitumor compound doxorubicin. The type II polyketide synthase (PKS) is a complex consisting of 5-10 standalone domains homologous to fatty acid synthase (FAS). Polyke...

journal_title：Biochemistry

authors： Korman TP,Tan YH,Wong J,Luo R,Tsai SC

更新日期：2008-02-19 00:00:00

abstract：:D-Xylose isomerase (XI) is a heat-stable homotetrameric enzyme used in industry for the production of high-fructose corn syrups by isomerization of D-glucose into D-fructose. To carry out biochemical and structural studies of this enzyme and of its engineered variants, a rapid and convenient method of purification of ...

journal_title：Biochemistry

authors： Mrabet NT

更新日期：1992-03-17 00:00:00

abstract：:Intestinal fatty acid binding protein (IFABP) and liver FABP (LFABP), homologous proteins expressed at high levels in intestinal absorptive cells, employ markedly different mechanisms for the transfer of fatty acids (FAs) to acceptor membranes. Transfer from IFABP occurs during protein-membrane collisional interaction...

journal_title：Biochemistry

authors： Córsico B,Liou HL,Storch J

更新日期：2004-03-30 00:00:00

abstract：:Tetrachlorohydroquinone dehalogenase catalyzes the replacement of chlorine atoms on tetrachlorohydroquinone and trichlorohydroquinone with hydrogen atoms during the biodegradation of pentachlorophenol by Sphingomonas chlorophenolica. The sequence of the active site region of tetrachlorohydroquinone dehalogenase is ver...

journal_title：Biochemistry

authors： Anandarajah K,Kiefer PM Jr,Donohoe BS,Copley SD

更新日期：2000-05-09 00:00:00

abstract：:The pH dependence of buffer catalysis of exchange of the C-4 amino protons of cyclic cytosine 2',3'-monophosphate (cCMP) and the N-1 proton of cyclic guanosine 2',3'-monophosphate (cGMP) conforms to an exchange mechanism, in which protonation of the nucleobases at C(N-3) AND G(N-7) establishes the important intermedia...

journal_title：Biochemistry

authors： McConnell B

更新日期：1978-07-25 00:00:00

abstract：:The complete amino acid sequence of human liver NADPH-cytochrome P-450 reductase has been determined by microsequence analysis and mass spectrometry. The total sequence consists of 676 amino acids initiated by an amino-terminal acetyl group. There is no evidence for posttranslational modifications, including Asn-linke...

journal_title：Biochemistry

authors： Haniu M,McManus ME,Birkett DJ,Lee TD,Shively JE

更新日期：1989-10-17 00:00:00

abstract：:Calmodulin (CaM) is an essential, eukaryotic protein comprised of two highly homologous domains (N and C). CaM binds four calcium ions cooperatively, regulating a wide array of target proteins. A genetic screen of Paramecia by Kung [Kung, C. et al. (1992) Cell Calcium 13, 413-425] demonstrated that the domains of CaM ...

journal_title：Biochemistry

authors： VanScyoc WS,Newman RA,Sorensen BR,Shea MA

更新日期：2006-12-05 00:00:00

abstract：:Synaptotagmin I is the calcium sensor in synchronous neurotransmitter release caused by fusion of synaptic vesicles with the presynaptic membrane in neurons. Synaptotagmin I interacts with acidic phospholipids, but also with soluble N-ethylmaleimide-sensitive factor attachment receptors (SNAREs), at various stages in ...

journal_title：Biochemistry

authors： Wan C,Kiessling V,Cafiso DS,Tamm LK

更新日期：2011-04-05 00:00:00

abstract：:Serine transacetylase from Salmonella typhimurium was purified to near homogeneity. A complete initial velocity study in both reaction directions suggests that the enzyme catalyzes the conversion of acetyl CoA and L-serine to O-acetyl-L-serine (OAS) and coenzyme A (CoASH) by a ping pong bi bi kinetic mechanism. Initia...

journal_title：Biochemistry

authors： Leu LS,Cook PF

更新日期：1994-03-08 00:00:00

abstract：:tRNA-guanine transglycosylase (TGT) is a key enzyme involved in the post-transcriptional modification of certain tRNAs in their anticodon wobble positions with queuine. To maintain the correct Watson-Crick base pairing properties of the wobble base (and hence proper translation of the genetic code), TGT must recognize...

journal_title：Biochemistry

authors： Todorov KA,Garcia GA

更新日期：2006-01-17 00:00:00

abstract：:Acyl-CoA:cholesterol O-acyltransferase (EC 2.3.1.26) (ACAT) catalyzes the intracellular synthesis of cholesteryl esters from cholesterol and fatty acyl-CoA at neutral pH. Despite the probable pathophysiologic role of ACAT in vascular cholesteryl ester accumulation during atherogenesis, its mechanism of action and its ...

journal_title：Biochemistry

authors： Kinnunen PM,DeMichele A,Lange LG

更新日期：1988-09-20 00:00:00

abstract：:The tau microtubule-associated proteins are axonal proteins that have been implicated in axonal outgrowth, microtubule spacing, and microtubule bundling. Moreover, tau is the major structural component of the paired helical filaments present in the brains of Alzheimer's disease patients. The Caenorhabditis elegans Gen...

journal_title：Biochemistry

authors： McDermott JB,Aamodt S,Aamodt E

更新日期：1996-07-23 00:00:00

abstract：:Whether hydrogen bonds between side chains are energetically significant in proteins and peptides has been controversial. A method is given here for measuring these interactions in peptide helices by comparing the helix contents of peptides with 1, 2, or 3 interactions. Results are given for the glutamine--aspartate (...

journal_title：Biochemistry

authors： Huyghues-Despointes BM,Klingler TM,Baldwin RL

更新日期：1995-10-17 00:00:00

abstract：:Trinucleotide repeat expansion diseases (TREDs) are correlated with elongation of CNG DNA and RNA repeats to pathological level. This paper shows, for the first time, complete data concerning thermodynamic stabilities of RNA with CNG trinucleotide repeats. Our studies include the stability of oligoribonucleotides comp...

journal_title：Biochemistry

authors： Broda M,Kierzek E,Gdaniec Z,Kulinski T,Kierzek R

更新日期：2005-08-16 00:00:00

abstract：:An in vitro system reconstituted with mouse liver polysome translation products was used to study the nature of polypeptide species imported into mitochondria from different mouse tissues such as liver, kidney, brain, and heart, as well as from Ehrlich ascites, Novikoff hepatoma, and Morris hepatoma 3924A tumor lines....

journal_title：Biochemistry

authors： Bhat NK,Avadhani NG

更新日期：1985-12-31 00:00:00

abstract：:Pea histones H2a, H2b, H3, and H4 have been isolated and their interactions studied by fluorescence anisotropy, light scatter, and circular dichroism. Histones H3 and H4 are almost identical in plants and animals, but plant histones H2a and H2b differ markedly from their mammalian counterparts. Pea H2b has a molecular...

journal_title：Biochemistry

authors： Spiker S,Isenberg I

更新日期：1977-05-03 00:00:00

abstract：:Aminoglycoside 2''-phosphotransferase IVa [APH(2'')-IVa] is a member of a family of bacterial enzymes responsible for medically relevant resistance to antibiotics. APH(2'')-IVa confers high-level resistance against several clinically used aminoglycoside antibiotics in various pathogenic Enterococcus species by phospho...

journal_title：Biochemistry

authors： Shi K,Houston DR,Berghuis AM

更新日期：2011-07-19 00:00:00

abstract：:In this work we modeled the circular dichroism (CD) spectrum of LHCII, the main light harvesting antenna of photosystem II of higher plants. Excitonic calculations are performed for a monomeric subunit, taken from the crystal structure of trimeric LHCII from spinach [Liu, Z. F., Yan, H. C., Wang, K. B., Kuang, T. Y., ...

journal_title：Biochemistry

authors： Georgakopoulou S,van der Zwan G,Bassi R,van Grondelle R,van Amerongen H,Croce R

更新日期：2007-04-24 00:00:00

abstract：:The present study was carried out to determine the energetics of Clostridium symbiosum pyruvate phosphate dikinase (PPDK) catalyzed interconversion of adenosine 5'-triphosphate (ATP), orthophosphate (Pi), and pyruvate (pyr) with adenosine 5'-monophosphate (AMP), inorganic pyrophosphate, and phosphoenolpyruvate (PEP) [...

journal_title：Biochemistry

authors： Mehl A,Xu Y,Dunaway-Mariano D

更新日期：1994-02-08 00:00:00

abstract：:The structural and functional roles of two conserved active site residues, Arg-97 and Arg-132, in chicken muscle adenylate kinase (AK) were evaluated by site-directed mutagenesis in conjunction with one- and two-dimensional proton nuclear magnetic resonance (NMR), kinetics, and guanidine hydrochloride-induced denatura...

journal_title：Biochemistry

authors： Dahnke T,Shi Z,Yan H,Jiang RT,Tsai MD

更新日期：1992-07-14 00:00:00

abstract：:Treatment of ADP-glucose pyrophosphorylase (EC 2.7.7.27) from the cyanobacterium Anabaena PCC 7120 with phenylglyoxal in 50 mM Hepes, pH 8.0, at 25 degrees C resulted in a time- and concentration-dependent loss of enzyme activity. Phosphate, the inhibitor, protected the enzyme from inactivation most effectively, while...

journal_title：Biochemistry

authors： Sheng J,Preiss J

更新日期：1997-10-21 00:00:00