Abstract：
:Pea histones H2a, H2b, H3, and H4 have been isolated and their interactions studied by fluorescence anisotropy, light scatter, and circular dichroism. Histones H3 and H4 are almost identical in plants and animals, but plant histones H2a and H2b differ markedly from their mammalian counterparts. Pea H2b has a molecular weight approximately 20% greater than that of calf thymus H2b; the amino acid compositions of the two proteins are different. Calf thymus H2a exists as a single molecular weight species, while pea H2a exists as two species which differ by about 1500 daltons. The larger plant H2a is about 19% greater in molecular weight than calf thymus H2a. The smaller is about 8% greater. Despite these differences between calf and pea histones, the strong interactions between histone pairs H3 and H4, H2b and H4, and H2a and H2b, previously demonstrated for calf histones, also exist for pea histones. There are also weak interactions between pea H2a and H4 and between pea H2b and H3, and an interaction of intermediate strength between H2a and H3. The cross-complexing pattern of the plant histones is therefore the same as that reported for calf thymus histones [D'Anna, J. A., Jr., and Isenberg, I. (1974), Biochemistry 13, 4992], despite the dissimilarities of H2a and H2b.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Spiker S,Isenberg Idoi
10.1021/bi00628a009subject
Has Abstractpub_date
1977-05-03 00:00:00pages
1819-26issue
9eissn
0006-2960issn
1520-4995journal_volume
16pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Spatially localized differences in the conformational dynamics of the rubredoxins from the hyperthermophile Pyrococcus furiosus (Pf) and the mesophile Clostridium pasteurianum (Cp) are monitored via amide exchange measurements. As shown previously for the hyperthermophile protein, nearly all backbone amides of the Cp ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0112560
更新日期：2001-12-04 00:00:00
abstract：:Direct chemical titrations of reduced, purified cytochrome aa3 were monitored at 604 nm. Anaerobic oxidation by potassium ferricyanide or 1,1'-bis(hydroxymethyl)-ferricinium ion was compared with the natural substrate, molecular oxygen. The four electrons from reduced cytochrome aa3 were donated to one oxygen molecule...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00626a014
更新日期：1977-04-05 00:00:00
abstract：:Based on the atomic structures of the mitochondrial cytochrome bc(1) complex, it has been proposed that the soluble domain of the [2Fe-2S] Rieske iron-sulfur protein (ISP) must rotate by ca. 60 degrees and translate through an appreciable distance between two binding sites, proximal to cytochrome c(1) and to the lumen...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9921992
更新日期：2000-03-14 00:00:00
abstract：:The marked difference between the intra- and extracellular 31P NMR chemical shifts of various phosphoryl compounds when added to a red cell suspension may be largely understood in terms of the effects of hemoglobin on the 31P NMR chemical shifts. The presence of [oxy- or (carbonmonoxy)-] hemoglobin inside the red cell...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00424a017
更新日期：1988-11-29 00:00:00
abstract：:Over 20 years ago, it was reported that liver cytosol contains at least two distinct proteins that transfer phosphatidylinositol in vitro, phosphatidylinositol transfer protein (PITP) and a pH 5.1 supernatant fraction containing sterol carrier protein-2 (SCP-2). In contrast to PITP, there has been minimal progress on ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi026904+
更新日期：2003-03-25 00:00:00
abstract：:The sialic acid N-acetylneuraminic acid (NANA) has a key role in the pathogenesis of a select number of neuroinvasive bacteria such as Neisseria meningitidis. These pathogens coat themselves with polysialic acids, mimicking the exterior surface of mammalian cells and consequentially concealing the bacteria from the ho...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi400062c
更新日期：2013-04-16 00:00:00
abstract：:The selectivity of binding of Escherichia coli RNA polymerase holoenzyme to a promoter-containing fragment of T7 DNA has been investigated over a range of solution conditions by using a double-label nitrocellulose filter binding assay. A 32P-labeled HaeIII restriction fragment of T7 D111 DNA containing the A1 and D pr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00556a014
更新日期：1980-07-22 00:00:00
abstract：:Seven hydrophobic residues ranging in size from glycine to phenylalanine have been substituted for the wild-type methionine residue at position 13 in a 15-residue truncated version (S15) of S-peptide, the small component of ribonuclease S. Complexes of both S-15 and the seven variants with S-protein yielded isomorphou...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00164a005
更新日期：1992-12-15 00:00:00
abstract：:Chemical-modification studies on submitochondrial particle pyridine dinucleotide transhydrogenase (EC 1.6.1.1) demonstrate the presence of one class of sulfhydryl group in the nicotinamide adenine dinucleotide phosphate (NADP) site and another peripheral to the active site. Reaction of the peripheral sulfhydryl group ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00615a015
更新日期：1978-10-31 00:00:00
abstract：:Aspartate transcarbamoylase from Escherichia coli is a dodecameric enzyme consisting of two trimeric catalytic subunits and three dimeric regulatory subunits. The X-ray structure of this enzyme indicates that the side chains of His-41, Asp-100, and Asp-90 from one catalytic chain form interactions with the side chains...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00089a034
更新日期：1993-09-28 00:00:00
abstract：:Earlier work indicates that the major DNA repair phosphodiesterase (PDE) in yeast cells is the well-characterized Apn1 protein. Apn1 demonstrates both Mg2+-independent PDE activity and Mg2+-independent class II apurinic/apyrimidinic (AP) endonuclease activity and represents greater than 90% of the activity detected in...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi970048y
更新日期：1997-05-20 00:00:00
abstract：:Cytochrome b5 reductase (cb5r), a member of the ferredoxin:NADP+ reductase family of flavoprotein transhydrogenases, catalyzes the NADH-dependent reduction of cytochrome b5. Within this family, a conserved "GxGxxP" sequence motif has been implicated in binding reduced pyridine nucleotides. However, Glycine 179, a cons...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi051165t
更新日期：2005-10-18 00:00:00
abstract：:Alditol oxidase (AldO) from Streptomyces coelicolor A3(2) is a soluble monomeric flavin-dependent oxidase that performs selective oxidation of the terminal primary hydroxyl group of several alditols. Here, we report the crystal structure of the recombinant enzyme in its native state and in complex with both six-carbon...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi701886t
更新日期：2008-01-22 00:00:00
abstract：:Ultrafast laser spectroscopy techniques are used to measure the low-frequency vibrational coherence spectra and nitric oxide rebinding kinetics of Caldariomyces fumago chloroperoxidase (CPO). Comparisons of the CPO coherence spectra with those of other heme species are made to gauge the protein-specific nature of the ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi7025485
更新日期：2008-05-06 00:00:00
abstract：:Kinetic models are presented that allow the Na(+),K(+)-ATPase steady-state turnover number to be estimated at given intra- and extracellular concentrations of Na(+), K(+), and ATP. Based on experimental transient kinetic data, the models utilize either three or four steps of the Albers-Post scheme, that is, E(2) --> E...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0355443
更新日期：2004-03-02 00:00:00
abstract：:Carboxyl ester lipase (CEL; EC 3.1.1.13) hydrolyzes cholesteryl esters and retinyl esters in vitro. In vivo, pancreatic CEL is thought to liberate cholesterol and retinol from their esters prior to absorption in the intestine. CEL is also a major lipase in the breast milk of many mammals, including humans and mice, an...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi981679a
更新日期：1999-03-30 00:00:00
abstract：:The Bacillus subtilis DNA-dependent RNA polymerase holoenzyme and core enzyme each contain approximately two atoms of zinc per molecule. When the dissociated subunits of the enzyme are passed through a blue dextran-Sepharose affinity column, only the beta subunit binds to the column. The total zinc content of the enzy...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00632a012
更新日期：1977-06-28 00:00:00
abstract：:In Gram-negative bacteria, the first step of lipid A biosynthesis is catalyzed by UDP-N-acetylglucosamine acyltransferase (LpxA) through the transfer of a R-3-hydroxyacyl chain from the acyl carrier protein (ACP) to the 3-hydroxyl group of UDP-GlcNAc. Previous studies suggest that LpxA is a critical determinant of the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00720
更新日期：2015-09-29 00:00:00
abstract：:It is generally accepted that electron transfer in bacterial photosynthesis is driven by the first singlet excited state of a special pair of bacteriochlorophylls (P*). We have examined the first steps of electron transfer in a mutant of the Rhodobacter sphaeroides reaction center in which charge separation from P* is...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9703756
更新日期：1997-06-10 00:00:00
abstract：:Fluorescence spectroscopy has been used to monitor the partitioning of a series of exchangeable neutral phospholipid probes, labeled with carbazole, indolyl or diphenylhexatrienyl moieties, between large unilamellar vesicles containing 1-palmitoyl-2-oleoylphosphatidylcholine (POPC), 1,2-dioleoyloxy-3-(trimethylammonio...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00244a018
更新日期：1991-07-30 00:00:00
abstract：:A plasminogen activator secreted by cultured human pancreatic carcinoma (Mia PaCa-2) cells has been purified to apparent homogeneity by procedures including Sepharose-L-arginine methyl ester affinity chromatography, Sephadex G-200 gel filtration, isoelectric focusing, and sodium dodecyl sulfate gel electrophoresis. Th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00628a023
更新日期：1977-05-03 00:00:00
abstract：:The Bordetella adenylate cyclase toxin-hemolysin (CyaA, ACT, or AC-Hly) forms cation-selective membrane channels and delivers into the cytosol of target cells an adenylate cyclase domain (AC) that catalyzes uncontrolled conversion of cellular ATP to cAMP. Both toxin activities were previously shown to depend on post-t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi050459b
更新日期：2005-09-27 00:00:00
abstract：:Complementary DNA clones coding for the human secreted carbonic anhydrase isozyme (CA VI) have been isolated and their nucleotide sequences determined. These clones identify a 1.45-kb mRNA that is present in high levels in parotid submandibular salivary glands but absent in other tissues such as the sublingual gland, ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00216a035
更新日期：1991-01-15 00:00:00
abstract：:Understanding the structural basis for defects in protein function that underlie protein-based genetic diseases is the fundamental requirement for development of therapies. This situation is epitomized by the cystic fibrosis transmembrane conductance regulator (CFTR)-the gene product known to be defective in CF patien...
journal_title：Biochemistry
pub_type： 杂志文章,评审
doi：10.1021/bi702209s
更新日期：2008-02-12 00:00:00
abstract：:The p53 protein family is involved in the control of an intricate network of genes implicated in cell cycle, through to germ line integrity and development. Although the role of p53 is well-established, the intrinsic nature of its homologue p73 has yet to be fully elucidated. Here, the biochemical characterization and...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi7023207
更新日期：2008-03-11 00:00:00
abstract：:Photolyases reverse the effects of UV light on cells by converting cyclobutane dipyrimidine photoproducts (pyrimidine dimers, Pyr mean value of Pyr) into pyrimidine monomers in a light-dependent reaction. Previous work has suggested that, based on substrate preference, there are two classes of photolyase: DNA photolya...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00099a019
更新日期：1991-09-03 00:00:00
abstract：:We report the use of anti-nucleoside antibodies to probe for local denaturation of calf thymus DNA upon binding of the antitumor drug cis-diamminedichloroplatinum(II), cis-DDP, and the biologically inactive analogues trans-diamminedichloroplatinum(II), trans-DDP, and chloro(diethylenetriamine)platinum(II) chloride, [P...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00355a008
更新日期：1986-04-08 00:00:00
abstract：:Early studies involving purine salvage in Salmonella typhimurium resulted in the isolation and identification of a mutant strain possessing a genetically modified hypoxanthine phosphoribosyl-transferase (HPRT) with enhanced substrate specificity for guanine [Benson, C. E., and Gots, J. S. (1975) J. Bacteriol. 121, 77-...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9720179
更新日期：1998-03-10 00:00:00
abstract：:Previous optical and electron paramagnetic resonance (EPR) spectroscopic studies of the newly discovered peroxynitrite scavenging pseudoperoxidase from Leishmania major (LmPP) suggested that ferric LmPP contained a six-coordinate low-spin (6cLS) heme with a thiolate ligand, presumably a cysteine, bound to its heme iro...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi401343t
更新日期：2013-12-10 00:00:00
abstract：:Lactose repressor protein, regulator of lac enzyme expression in Escherichia coli, maintains its structure and function at extremely low protein concentrations (<10(-)12 M). To examine the unfolding and dissociation of this tetrameric protein, structural transitions in the presence of varying concentrations of urea we...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9900727
更新日期：1999-05-18 00:00:00
