Chemical basis for the selectivity of the von Hippel Lindau tumor suppressor pVHL for prolyl-hydroxylated HIF-1alpha.

abstract：:Prion diseases are associated with a conformational switch in the prion protein (PrP) from its normal cellular form (denoted PrP(C)) to a disease-associated "scrapie" form (PrP(Sc)). A number of PrP(Sc)-like conformations can be generated by incubating recombinant PrP(C) at low pH, indicating that protonation of key r...

journal_title：Biochemistry

authors： Hosszu LL,Tattum MH,Jones S,Trevitt CR,Wells MA,Waltho JP,Collinge J,Jackson GS,Clarke AR

更新日期：2010-10-12 00:00:00

abstract：:Sedimentation and gel retardation studies show a stronger interaction of HMG 1 and 2 with negatively supercoiled DNA than with linear, nicked-circular, or positively supercoiled ds-DNA. An apparent unwinding angle of 58 degrees was obtained for HMG 1 and 2 when assayed by protection of negatively supercoiled DNA from ...

journal_title：Biochemistry

authors： Sheflin LG,Fucile NW,Spaulding SW

更新日期：1993-04-06 00:00:00

abstract：:Horseradish peroxidase (HRP) was oxidized by IrCl6(2-) to a mixture of compounds I and II, the rate of oxidation and the ratio of the mixture being greatly affected by pH (Hayashi & Yamazaki, 1979). Oxidation of HRP by IrCl6(2-) in the presence of fluoride was significantly accelerated. This resulted in the formation ...

journal_title：Biochemistry

authors： Farhangrazi ZS,Sinclair R,Powers L,Yamazaki I

更新日期：1995-11-21 00:00:00

abstract：:The azinomycins are a family of potent antitumor agents with the ability to form interstrand cross-links with DNA. This study reports on the unusual biosynthetic formation of the 5-methyl naphthoate moiety, which is essential for effective DNA association. While sequence analysis predicts that the polyketide synthase ...

journal_title：Biochemistry

authors： Mori S,Simkhada D,Zhang H,Erb MS,Zhang Y,Williams H,Fedoseyenko D,Russell WK,Kim D,Fleer N,Ealick SE,Watanabe CM

更新日期：2016-02-02 00:00:00

abstract：:Currently, the relatively high cost of enzymes such as glycoside hydrolases that catalyze cellulose hydrolysis represents a barrier to commercialization of a biorefinery capable of producing renewable transportable fuels such as ethanol from abundant lignocellulosic biomass. Among the many families of glycoside hydrol...

journal_title：Biochemistry

authors： Harris PV,Welner D,McFarland KC,Re E,Navarro Poulsen JC,Brown K,Salbo R,Ding H,Vlasenko E,Merino S,Xu F,Cherry J,Larsen S,Lo Leggio L

更新日期：2010-04-20 00:00:00

abstract：:The evolutionarily conserved DRY motif at the end of the third helix of rhodopsin-like, class-A G protein-coupled receptors (GPCRs) is a major regulator of receptor stability, signaling activity, and β-arrestin-mediated internalization. Substitution of the DRY arginine with histidine in the human vasopressin receptor ...

journal_title：Biochemistry

authors： Burczyk M,Burkhalter MD,Blätte T,Matysik S,Caron MG,Barak LS,Philipp M

更新日期：2015-01-27 00:00:00

abstract：:The ATPase activity of the (Ca2+-Mg2+)-ATPase reconstituted into bilayers of phosphatidylcholines depends on the fatty acyl chain length of the phospholipids. It is shown that the fluorescence response to Ca2+ of the ATPase modified with fluorescein isothiocyanate is also dependent on phospholipid structure and is int...

journal_title：Biochemistry

authors： Froud RJ,East JM,Jones OT,Lee AG

更新日期：1986-11-18 00:00:00

abstract：:We have investigated the loss of histone H1 from chromatin [D'Anna, J. A., Gurley, L. R., & Tobey, R. A. (1982) Biochemistry 21, 3991-4001] and the structure of chromatin from Chinese hamster (line CHO) cells blocked in early S phase by sequential use of isoleucine deprivation G1 block and 1 mM hydroxyurea (HU) blocka...

journal_title：Biochemistry

authors： D'Anna JA,Prentice DA

更新日期：1983-11-22 00:00:00

abstract：:Seipin is a transmembrane protein that resides in the endoplasmic reticulum and concentrates at junctions between the ER and cytosolic lipid droplets. Mutations in the human seipin gene, including the missense mutation A212P, lead to congenital generalized lipodystrophy (CGL), characterized by the lack of normal adipo...

journal_title：Biochemistry

authors： Binns D,Lee S,Hilton CL,Jiang QX,Goodman JM

更新日期：2010-12-21 00:00:00

abstract：:The innate immune response to infection or injury involves an antigen-antibody triggered classical pathway (CP) of complement activation, in which soluble and cell surface plasma proteins cooperatively effect elimination of foreign organisms and damaged host cells. However, protracted or dysfunctional complement activ...

journal_title：Biochemistry

authors： Halili MA,Ruiz-Gómez G,Le GT,Abbenante G,Fairlie DP

更新日期：2009-09-08 00:00:00

abstract：:Chicken liver phosphoenolpyruvate carboxykinase (PEPCK) is activated by Cr2+ as the sole activator under anaerobic conditions. PEPCK was modified with Cr3+, starting with either Cr2+ or Cr3+. Cr3+ has the distinct advantage of being a paramagnetic cation that could serve as a paramagnetic probe. Activators Mn2+, Mg2+,...

journal_title：Biochemistry

authors： Hlavaty JJ,Nowak T

更新日期：1998-06-02 00:00:00

abstract：:The behavior of photosystem II (PSII) reactions was investigated under conditions of decreasing water content by the addition of increasing concentrations of ethylene glycol (EG). The photosynthetic activities were measured for PSII samples either directly in aqueous solutions of EG or in the standard buffer medium fo...

journal_title：Biochemistry

authors： Hillier W,Lukins P,Seibert M,Wydrzynski T

更新日期：1997-01-07 00:00:00

abstract：:The thylakoid transmembrane DeltapH is the sole energy source driving translocation of precursor proteins by the DeltapH/Tat machinery. Consequently, proton translocation must be coupled to precursor translocation. For the precursor of the 17 kDa protein of the oxygen-evolving complex (pOE17), the protein translocatio...

journal_title：Biochemistry

authors： Musser SM,Theg SM

更新日期：2000-07-18 00:00:00

abstract：:Alpha-synuclein modulates dopamine homeostasis in dopamine-producing neurons of substantia nigra, partly through regulation of human dopamine transporter (hDAT) activity. To identify the underlying mechanisms, we disrupted the modulation of hDAT activity by wild-type (wt) alpha-synuclein, and its familial Parkinson's ...

journal_title：Biochemistry

authors： Wersinger C,Vernier P,Sidhu A

更新日期：2004-02-10 00:00:00

abstract：:Stopped-flow total intensity and anisotropy experiments on single tryptophan containing mutants of yeast phosphoglycerate kinase (PGK) located in either the carboxy-terminal domain (W308 and W333), amino-terminal domain (W48 and W122), or "hinge" region (W194 and W399) were performed. The results obtained for single t...

journal_title：Biochemistry

authors： Beechem JM,Sherman MA,Mas MT

更新日期：1995-10-24 00:00:00

abstract：:To test whether the Y-shaped conformation of the hammerhead ribozyme is maintained throughout the catalytic pathway, the cleavage properties of circular substrates which bind the ribozyme through helices I and II were determined. Constraining the position of helices I and II in this manner did not significantly alter ...

journal_title：Biochemistry

authors： Stage-Zimmermann TK,Uhlenbeck OC

更新日期：1998-06-30 00:00:00

abstract：:Here, we biochemically characterize Dictyostelium myosin II mutants that were previously phenotypically selected following random mutagenesis and shown to lie in the actin binding domain [Patterson, B., & Spudich, J. A. (1996) Genetics 143, 801-810]. We show that the conditional loss of myosin-dependent activity in vi...

journal_title：Biochemistry

authors： Giese KC,Spudich JA

更新日期：1997-07-15 00:00:00

abstract：:The repair of the endogenous lesion 8-oxo-7,8-dihydrodeoxyguanosine (8-oxodG) was investigated in the nucleotide excision repair mutant xeroderma pigmentosum D (XPD), using human normal or transformed XPD fibroblasts and the Chinese hamster XPD cell line UV5. In vivo repair of 8-oxodG induced by hydrogen peroxide trea...

journal_title：Biochemistry

authors： Cappelli E,Degan P,Thompson LH,Frosina G

更新日期：2000-08-29 00:00:00

abstract：:Elongation factor-2 kinase (eEF-2K) is a Ca(2+)/calmodulin-dependent protein kinase that phosphorylates and inactivates eEF-2 and that can regulate the rate of protein synthesis at the elongation stage. Here we report that a slight decrease in pH, within the range observed in vivo, leads to a dramatic activation of eE...

journal_title：Biochemistry

authors： Dorovkov MV,Pavur KS,Petrov AN,Ryazanov AG

更新日期：2002-11-12 00:00:00

abstract：:The 18 kDa translocator protein (TSPO), formerly known as the peripheral-type benzodiazepine receptor, has been reported to be closely associated with the mitochondrial permeability transition pore (MPTP). TSPO is believed to exert pro-apoptotic functions via modulation of MPTP opening. Cobalt chloride (CoCl(2)), whic...

journal_title：Biochemistry

authors： Zeno S,Zaaroor M,Leschiner S,Veenman L,Gavish M

更新日期：2009-06-02 00:00:00

abstract：:Human hemoglobin produced in the Escherichia coli coexpression system of Hernan et al. [(1992) Biochemistry 31, 8619-8628] has been transformed into a functionally homogeneous protein whose properties closely approximate those of normal hemoglobin A. Both of the alpha and beta chains of this hemoglobin contain a valin...

journal_title：Biochemistry

authors： Hui HL,Kavanaugh JS,Doyle ML,Wierzba A,Rogers PH,Arnone A,Holt JM,Ackers GK,Noble RW

更新日期：1999-01-19 00:00:00

abstract：:The binding of various forms of vanadate to myosin and myosin subfragment 1 (S-1) was studied by 51V NMR at increasing vanadate concentrations between 0.06 and 1.0 mM. The distribution of the various forms of vanadate in the solution depended on the total concentration of vanadate. At low concentrations, the predomina...

journal_title：Biochemistry

authors： Ringel I,Peyser YM,Muhlrad A

更新日期：1990-09-25 00:00:00

abstract：:The dynamics of the enthalpy and volume changes found in the photodissociation of CO from sperm whale carboxymyoglobin and two site-directed mutants in which arginine-45 is replaced by glycine and asparagine are examined by photoacoustic calorimetry. An intermediate is observed whose lifetime at 20 degrees C is 700 ns...

journal_title：Biochemistry

authors： Westrick JA,Peters KS,Ropp JD,Sligar SG

更新日期：1990-07-17 00:00:00

abstract：:Remodeling of photosynthetic machinery induced by growing spinach plants under low light intensities reveals an up-regulation of light-harvesting complexes and down-regulation of photosystem II and cytochrome b6f complexes in intact thylakoids and isolated grana membranes. The antenna size of PSII increased by 40-60% ...

journal_title：Biochemistry

authors： Kirchhoff H,Haase W,Wegner S,Danielsson R,Ackermann R,Albertsson PA

更新日期：2007-10-02 00:00:00

abstract：:Many bacteria produce isonitrile-containing natural products that are derived from aromatic amino acids. The synthetic clusters that control biosynthesis most commonly encode two enzymes, designated PvcA and PvcB, as well as additional enzymes that direct synthesis of the natural product. The PvcA enzyme installs the ...

journal_title：Biochemistry

authors： Zhu J,Lippa GM,Gulick AM,Tipton PA

更新日期：2015-04-28 00:00:00

abstract：:The crystal structures of metyrapone- and 1-, 2-, and 4-phenylimidazole-inhibited complexes of cytochrome P-450cam have been refined to a nominal resolution of 2.1 A and compared with the 1.63-A camphor-bound structure. With the exception of 2-phenylimidazole, each of the inhibitors forms an N-Fe bond with the heme ir...

journal_title：Biochemistry

authors： Poulos TL,Howard AJ

更新日期：1987-12-15 00:00:00

abstract：:Conformational and environmental changes in the functionally significant amino-terminal region of human parathyroid hormone (hPTH), induced by solvent or by complexation with acidic lipid, have been investigated. Structural perturbations were monitored by their effect on the fluorescence decay kinetics of the single t...

journal_title：Biochemistry

authors： Willis KJ,Szabo AG

更新日期：1992-09-22 00:00:00

abstract：:Apo-electron-transferring flavoprotein from pig kidney (apo-ETF) has been prepared by an acid ammonium sulfate procedure and reconstituted with FAD analogues to probe the flavin binding site. The 8-position of the bound flavin is accessible to solvent as judged by the reaction of 8-Cl-FAD-ETF with sodium sulfide and t...

journal_title：Biochemistry

authors： Gorelick RJ,Thorpe C

更新日期：1986-11-04 00:00:00

abstract：:Mutations in the hydrophobic interior of proteins are generally thought to weaken the interactions only in their immediate neighborhood. This forms the basis of protein engineering-based studies of folding mechanism and function. However, mutational work on diverse proteins has shown that distant residues are thermody...

journal_title：Biochemistry

authors： Rajasekaran N,Suresh S,Gopi S,Raman K,Naganathan AN

更新日期：2017-01-10 00:00:00

abstract：:Factor X (FX) is one of the major players in the blood coagulation cascade. Upon activation to FXa, it converts prothrombin to thrombin, which in turn converts fibrinogen into fibrin (blood clots). FXa deficiency causes hemostasis defects, such as intracranial bleeding, hemathrosis, and gastrointestinal blood loss. He...

journal_title：Biochemistry

authors： Abdel-Azeim S,Oliva R,Chermak E,De Cristofaro R,Cavallo L

更新日期：2014-11-11 00:00:00