Polyketide Ring Expansion Mediated by a Thioesterase, Chain Elongation and Cyclization Domain, in Azinomycin Biosynthesis: Characterization of AziB and AziG.

abstract：:The interaction of the Escherichia coli single-stranded DNA binding protein (SSB) with oligo- and poly-nucleotides has been studied by 270-MHz 1H NMR spectroscopy and fast kinetic techniques. d(pT)8 and poly(dT) were used to study noncooperative and cooperative binding, respectively. The H6, H1', and CH3 resonances of...

journal_title：Biochemistry

authors： Römer R,Schomburg U,Krauss G,Maass G

更新日期：1984-12-04 00:00:00

abstract：:The nuclear tRNA 3' processing activity from wheat has been characterized and partially purified. Several characteristics of the wheat nuclear 3' processing enzyme now allow this activity to be distinguished from its mitochondrial counterpart. The nuclear enzyme is an endonuclease, which we termed nuclear RNase Z. The...

journal_title：Biochemistry

authors： Mayer M,Schiffer S,Marchfelder A

更新日期：2000-02-29 00:00:00

abstract：:The interplay between the early collapse of the unfolded state and the formation of the secondary structure has been the subject of extensive research in protein chemistry. In this study, we used the intestinal fatty acid binding protein (IFABP), a small model protein with predominately β-sheet structure, to study the...

journal_title：Biochemistry

authors： Sarkar S,Chattopadhyay K

更新日期：2014-03-11 00:00:00

abstract：:Multielectron multiproton reactions play an important role in both biological systems and chemical reactions involved in energy storage and manipulation. A key strategy employed by nature in achieving such complex chemistry is the use of proton-coupled redox steps. Cytochrome c nitrite reductase (ccNiR) catalyzes the ...

journal_title：Biochemistry

authors： Judd ET,Stein N,Pacheco AA,Elliott SJ

更新日期：2014-09-09 00:00:00

abstract：:We investigated the molecular mechanisms and the binding site location for the fluorophor crystal violet (CrV), a noncompetitive antagonist of the nicotinic acetylcholine receptor (AChR). To this end, radiolabeled competition binding, fluorescence spectroscopy, Schild-type analysis, patch-clamp recordings, and molecul...

journal_title：Biochemistry

authors： Arias HR,Bhumireddy P,Spitzmaul G,Trudell JR,Bouzat C

更新日期：2006-02-21 00:00:00

abstract：:Cathepsin C, or dipeptidyl peptidase I, is a lysosomal cysteine protease of the papain family that catalyzes the sequential removal of dipeptides from the free N-termini of proteins and peptides. Using the dipeptide substrate Ser-Tyr-AMC, cathepsin C was characterized in both steady-state and pre-steady-state kinetic ...

journal_title：Biochemistry

authors： Schneck JL,Villa JP,McDevitt P,McQueney MS,Thrall SH,Meek TD

更新日期：2008-08-19 00:00:00

abstract：:The effect of pretreatment with sodium butyrate on DNA excision repair was studied in intact and permeable confluent (i.e., growth-inhibited) diploid human fibroblasts. Exposure to 20 mM sodium butyrate for 48 h increased subsequent ultraviolet (UV)-induced [methyl-3H]thymidine incorporation by intact AG1518 fibroblas...

journal_title：Biochemistry

authors： Dresler SL

更新日期：1985-11-19 00:00:00

abstract：:Group VIA phospholipase A(2) (iPLA(2)beta) is expressed in phagocytes, vascular cells, pancreatic islet beta-cells, neurons, and other cells and plays roles in transcriptional regulation, cell proliferation, apoptosis, secretion, and other events. A bromoenol lactone (BEL) suicide substrate used to study iPLA(2)beta f...

journal_title：Biochemistry

authors： Song H,Bao S,Ramanadham S,Turk J

更新日期：2006-05-23 00:00:00

abstract：:The third and most recently identified Parkinson's disease-linked variant of the neuronal protein alpha-synuclein to be identified (E46K) results in widespread brain pathology and early onset Parkinson symptoms (Zarranz et al. (2004) Ann. Neurol. 55, 164-173). Herein, we present biochemical and biophysical characteriz...

journal_title：Biochemistry

authors： Fredenburg RA,Rospigliosi C,Meray RK,Kessler JC,Lashuel HA,Eliezer D,Lansbury PT Jr

更新日期：2007-06-19 00:00:00

abstract：:Two hybrid ribulose-1,5 bisphosphate carboxylase/oxygenase (RubisCO) enzymes were constructed using RubisCO small subunit genes (rbcS) from two eucaryotic marine organisms, Cylindrotheca sp. N1 and Olisthodiscus luteus, cloned downstream of the RubisCO large subunit gene (rbcL) of the cyanobacterium Synechococcus PCC ...

journal_title：Biochemistry

authors： Read BA,Tabita FR

更新日期：1992-06-23 00:00:00

abstract：:The sterol (free and esterified cholesterol) transport activity of the small-intestinal brush border membrane was solubilized with the short-chain detergent diheptanoylphosphatidylcholine and reconstituted to an artificial membrane system (proteoliposomes). The resulting proteoliposomes were identified as unilamellar ...

journal_title：Biochemistry

authors： Boffelli D,Weber FE,Compassi S,Werder M,Schulthess G,Hauser H

更新日期：1997-09-02 00:00:00

abstract：:A putative catalytic triad consisting of tyrosine, serine, and lysine residues was identified in the ketoreductase (KR) domains of modular polyketide synthases (PKSs) based on homology modeling to the short chain dehydrogenase/reductase (SDR) superfamily of enzymes. This was tested by constructing point mutations for ...

journal_title：Biochemistry

authors： Reid R,Piagentini M,Rodriguez E,Ashley G,Viswanathan N,Carney J,Santi DV,Hutchinson CR,McDaniel R

更新日期：2003-01-14 00:00:00

abstract：:Thymidylate synthetase catalyzes an exchange of tritium of [5-3H]dUMP for protons of water in the absence of CH2-H4folate. The turnover number for this reaction is some 45,000-fold lower than that of dTMP formation and Km is 1.2 X 10(-5) M, similar to the dissociation constant of the enzyme-dUMP complex determined by ...

journal_title：Biochemistry

authors： Pogolotti AL Jr,Weill C,Santi DV

更新日期：1979-06-26 00:00:00

abstract：:The melanocortin-4 (MC4) receptor is a potential therapeutic target for obesity and cachexia, for which nonpeptide agonists and antagonists are being developed, respectively. The aim of this study was to identify molecular interactions between the MC4 receptor and nonpeptide ligands, and to compare the mechanism of bi...

journal_title：Biochemistry

authors： Fleck BA,Chen C,Yang W,Huntley R,Markison S,Nickolls SA,Foster AC,Hoare SR

更新日期：2005-11-08 00:00:00

abstract：:A new method of calculation, based on a direct fitting of the protein fluorescence intensity observed upon coenzyme binding (H.-P. Lutz, unpublished results), is used to study the negative cooperative behavior of glyceraldehyde-3-phosphate dehydrogenase from sturgeon muscle. The calculation procedure simultaneously el...

journal_title：Biochemistry

authors： Branlant G,Eiler B,Biellmann JF,Lutz HP,Luisi PL

更新日期：1983-09-13 00:00:00

abstract：:The luminescence of Cu-substituted cytochrome c exhibits temperature-dependent decay modes and spectral shifts at temperatures below 80 K. By comparison with the data from metal-substituted porphyrins, we determined intramolecular rate parameters. The observed tripdoublet decay time is 13 +/- 1 mus at 77 K and the qua...

journal_title：Biochemistry

authors： Glatz P,Chance B,Vanderkooi JM

更新日期：1979-08-07 00:00:00

abstract：:The lateral and rotational dynamics of phosphoinositides and their interactions with proteins were characterized using pyrene-labeled lipid analogues. In these systems, the collision frequency of pyrene-labeled lipids was studied by monitoring the monomeric pyrene fluorescence yield as a function of their mole fractio...

journal_title：Biochemistry

authors： Pap EH,Hanicak A,van Hoek A,Wirtz KW,Visser AJ

更新日期：1995-07-18 00:00:00

abstract：:The mechanism of binding of the substrates Mg x ATP and ribose 5-phosphate as well as Mg2+ to the enzyme 5-phospho-D-ribosyl (alpha-1-diphosphate synthetase from Escherichia coli has been analyzed. By use of the competive inhibitors of ATP and ribose 5-phosphate binding, alpha,beta-methylene ATP and (+)-1-alpha,2-alph...

journal_title：Biochemistry

authors： Willemoës M,Hove-Jensen B

更新日期：1997-04-22 00:00:00

abstract：:Simple two-dimensional thin-layer chromatography was found to be useful for the separation of sugar methylated dinucleotides in RNA. Of the 16 possible sequences of the type Nm-Np, 15 were separated and all the sequences were determined. In a mouse hepatoma, MH 134, the levels of the sugar methylation in the 18S and 2...

journal_title：Biochemistry

authors： Hashimoto S,Sakai M,Muramatsu M

更新日期：1975-05-06 00:00:00

abstract：:Phospholipase B1 (PLB1), secreted by the pathogenic yeast Cryptococcus neoformans, has an established role in virulence. Although the mechanism of its phospholipase B, lysophospholipase, and lysophospholipase transacylase activities is unknown, it possesses lipase, subtilisin protease aspartate, and phospholipase moti...

journal_title：Biochemistry

authors： Jones PM,Turner KM,Djordjevic JT,Sorrell TC,Wright LC,George AM

更新日期：2007-09-04 00:00:00

abstract：:Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4) is a thermostable archaeal enzyme and a member of the error-prone and lesion-bypass Y-family. In this paper, for the first time, the fidelity of a Y-family polymerase, Dpo4, was determined using pre-steady-state kinetic analysis of the incorporation of a single nucle...

journal_title：Biochemistry

authors： Fiala KA,Suo Z

更新日期：2004-02-24 00:00:00

abstract：:The dynamic cytoskeletal network of microtubules and actin filaments can be disassembled by drugs. Cytoskeletal drugs work by perturbing the monomer-polymer equilibrium, thus changing the size and number of macromolecular crowders inside cells. Changes in both crowding and nonspecific surface interactions ("sticking")...

journal_title：Biochemistry

authors： Davis CM,Gruebele M

更新日期：2020-07-21 00:00:00

abstract：:Several snake venoms contain a phospholipase A2 in which position 49 in the active site is occupied by a lysine or a serine instead of the aspartate residue normally found. Although these proteins do not bind Ca2+ and are devoid of catalytic activity, they are still highly specific myotoxins and have recently been sho...

journal_title：Biochemistry

authors： Pedersen JZ,Lomonte B,Massoud R,Gubensek F,Gutiérrez JM,Rufini S

更新日期：1995-04-11 00:00:00

abstract：:Subfragment 1 (S1) prepared from rabbit skeletal muscle myosin was digested with trypsin to cleave the 95K heavy chain into three pieces, i.e., the 23K, 50K, and 20K fragments. The trypsin-treated S1 was then cross-linked with p-nitrophenyl iodoacetate. The cross-linker bridged one of the reactive thiols (SH2) in the ...

journal_title：Biochemistry

authors： Sutoh K,Hiratsuka T

更新日期：1988-04-19 00:00:00

abstract：:Serine palmitoyltransferase (EC 2.3.1.50) catalyzes the condensation of L-serine and palmitoyl-CoA to yield 3-ketosphinganine in the first unique reaction of long-chain (sphingoid) base biosynthesis. The kinetic effects of changing the extracellular concentrations of the precursors for this pathway were studied with L...

journal_title：Biochemistry

authors： Merrill AH Jr,Wang E,Mullins RE

更新日期：1988-01-12 00:00:00

abstract：:Amyloid-beta (Abeta) peptide deposition as fibrillar senile plaques is a key element in the pathology of Alzheimer's disease. Here we present a high-resolution structure of an Abeta amyloid fibril using magnetically aligned preparations of a central Abeta domain which forms representative amyloid fibrils. Diffraction ...

journal_title：Biochemistry

authors： Serpell LC,Blake CC,Fraser PE

更新日期：2000-10-31 00:00:00

abstract：:35S-labeled derivatives of the insecticides nodulisporic acid and ivermectin were synthesized and demonstrated to bind with high affinity to a population of receptors in Drosophila head membranes that were previously shown to be associated with a glutamate-gated chloride channel. Nodulisporic acid binding was modeled ...

journal_title：Biochemistry

authors： Ludmerer SW,Warren VA,Williams BS,Zheng Y,Hunt DC,Ayer MB,Wallace MA,Chaudhary AG,Egan MA,Meinke PT,Dean DC,Garcia ML,Cully DF,Smith MM

更新日期：2002-05-21 00:00:00

abstract：:Peptidylglycine monooxygenase (PHM) catalyzes the final step in the biosynthesis of amidated peptides that serve as important signaling molecules in numerous endocrine pathways. The catalytic mechanism has attracted much attention because of a number of unique attributes, including the presence of a pair of uncoupled ...

journal_title：Biochemistry

authors： Chauhan S,Kline CD,Mayfield M,Blackburn NJ

更新日期：2014-02-18 00:00:00

abstract：:Protein binding specificities can be manipulated by redesigning contacts that already exist at an interface or by expanding the interface to allow interactions with residues adjacent to the original binding site. Previously, we developed a strategy, called AnchorDesign, for expanding interfaces around linear binding e...

journal_title：Biochemistry

authors： Hussain M,Angus SP,Kuhlman B

更新日期：2018-07-31 00:00:00

abstract：:A peptide consisting of the first 21 residues of human myelin basic protein (MBP) was synthesized. The N-terminal alanine of portions was blocked in separate experiments with alkyl carboxylates varying in size from 2 to 10 carbon atoms. The effects of these different alkyl carboxylates at the N-terminus on the seconda...

journal_title：Biochemistry

authors： Costentino M,Pritzker L,Boulias C,Moscarello MA

更新日期：1994-04-12 00:00:00