Tertiary and quaternary allostery in tetrameric hemoglobin from Scapharca inaequivalvis.

abstract：:The proton nuclear magnetic resonance spectrum of human adult deoxyhemoglobin in D2O in the region from 6 to 20 ppm downfield from the proton resonance of residual water shows a number of hyperfine shifted proton resonances that are due to groups on or near the alpha and beta hemes. The sensitivity of these resonances...

journal_title：Biochemistry

authors： Viggiano G,Ho NT,Ho C

更新日期：1979-11-13 00:00:00

abstract：:YtvA is a blue light sensor protein composed of an N-terminal LOV (light-oxygen-voltage) domain, a linker helix, and the C-terminal sulfate transporter and anti-σ factor antagonist domain. YtvA is believed to act as a positive regulator for light and salt stress responses by regulating the σB transcription factor. Alt...

journal_title：Biochemistry

authors： Choi S,Nakasone Y,Hellingwerf KJ,Terazima M

更新日期：2016-06-07 00:00:00

abstract：:Filamins are a family of actin cross-linking proteins that are primarily localized in the cortical cytoplasm of all mammalian cells. Until now, three major isoforms (filamins a, b, and c) have been identified, that were shown to be differentially expressed and/or localized in different tissues. An amino-terminal doubl...

journal_title：Biochemistry

authors： Himmel M,Van Der Ven PF,Stöcklein W,Fürst DO

更新日期：2003-01-21 00:00:00

abstract：:Conformational states of the Na,K-ATPase and rates of transition between these are studied using the fluorescent dye eosin as a marker for the Na+ form (E1) and occlusion of 86Rb+ as a marker for the K+ form (E2). The aim of the present paper is to propose that the E1 form of the Na,K-ATPase can be liganded with a num...

journal_title：Biochemistry

authors： Esmann M

更新日期：1994-07-19 00:00:00

abstract：:A heterologous expression system was used to evaluate activation of BlaR1, a sensor/signal transducer protein of Staphylococcus aureus with a central role in resistance to β-lactam antibiotics. In the absence of other S. aureus proteins that might respond to antibiotics and participate in signal transduction events, w...

journal_title：Biochemistry

authors： Llarrull LI,Mobashery S

更新日期：2012-06-12 00:00:00

abstract：:The initial step of infection of blood cells with the human immunodeficiency virus, HIV, is the formation of a complex of the viral envelope protein gp120 and its human receptor CD4. We have examined structural features of recombinant soluble CD4 (sCD4) by chemical modification of arginine residues with hydroxyphenylg...

journal_title：Biochemistry

authors： Hager-Braun C,Tomer KB

更新日期：2002-02-12 00:00:00

abstract：:The addition of 10(-9) M insulin to a suspension of rat liver plasma membranes increases the overall lipid microviscosity, eta, by about 10--20%. The effect is confined to physiological concentrations of the hormone and is highly specific. The specificity was demonstrated in experiments where insulin analogues were ad...

journal_title：Biochemistry

authors： Luly P,Shinitzky M

更新日期：1979-02-06 00:00:00

abstract：:Cofilin is an actin regulatory protein that binds to both monomeric and filamentous actin, and has filament severing activity. Although crystal structures for the monomeric forms of both G-actin and cofilin have been described, the structure of the binary cofilin-G-actin complex is not available. Synchrotron protein f...

journal_title：Biochemistry

authors： Guan JQ,Vorobiev S,Almo SC,Chance MR

更新日期：2002-05-07 00:00:00

abstract：:Integrin αIIbβ3, a transmembrane heterodimer, mediates platelet aggregation when it switches from an inactive to an active ligand-binding conformation following platelet stimulation. Central to regulating αIIbβ3 activity is the interaction between the αIIb and β3 extracellular stalks, which form a tight heterodimer in...

journal_title：Biochemistry

authors： Tan SK,Fong KP,Polizzi NF,Sternisha A,Slusky JSG,Yoon K,DeGrado WF,Bennett JS

更新日期：2019-07-30 00:00:00

abstract：:Specific interactions of human melanocortin-4 receptor (hMC4R) with its nonpeptide and peptide agonists were studied using alanine-scanning mutagenesis. The binding affinities and potencies of two synthetic, small-molecule agonists (THIQ, MB243) were strongly affected by substitutions in transmembrane alpha-helices (T...

journal_title：Biochemistry

authors： Pogozheva ID,Chai BX,Lomize AL,Fong TM,Weinberg DH,Nargund RP,Mulholland MW,Gantz I,Mosberg HI

更新日期：2005-08-30 00:00:00

abstract：:Epidermal growth factor receptor (EGFR) exemplifies the family of receptor tyrosine kinases that mediate numerous cellular processes, including growth, proliferation, and differentiation. Moreover, gene amplification and EGFR mutations have been identified in a number of human malignancies, making this receptor an imp...

journal_title：Biochemistry

authors： Truong TH,Carroll KS

更新日期：2012-12-18 00:00:00

abstract：:The activity of serine carboxypeptidases is dependent on a catalytic triad, an oxyanion hole, and a binding site equivalent to those found in the serine endopeptidases. The action of carboxypeptidase Y on substrates containing amino acids, alcohols, and amines as leaving groups is described. It is demonstrated that th...

journal_title：Biochemistry

authors： Stennicke HR,Mortensen UH,Breddam K

更新日期：1996-06-04 00:00:00

abstract：:Human lysosomal enzymes acid-beta-glucosidase (GCase) and acid-alpha-galactosidase (alpha-Gal A) hydrolyze the sphingolipids glucosyl- and globotriaosylceramide, respectively, and mutations in these enzymes lead to the lipid metabolism disorders Gaucher and Fabry disease, respectively. We have investigated the structu...

journal_title：Biochemistry

authors： Lieberman RL,D'aquino JA,Ringe D,Petsko GA

更新日期：2009-06-09 00:00:00

abstract：:Galactosyl- and glucosylceramide, globoside, and dihydrolactosylceramide, bearing [2,2-2H2]stearic acid, have been studied at a concentration of 10 mol% in bilayers of dimyristoylphosphatidylcholine by 2H NMR. The quadrupolar splitting delta vQ of the C2 deuterons were measured at several temperatures in the range of ...

journal_title：Biochemistry

authors： Fenske DB,Hamilton K,Jarrell HC,Florio E,Barber KR,Grant CW

更新日期：1991-05-07 00:00:00

abstract：:The purL gene of Escherichia coli encoding the enzyme formylglycinamidine ribonucleotide (FGAM) synthetase which catalyzes the conversion of formylglycinamide ribonucleotide (FGAR), glutamine, and MgATP to FGAM, glutamate, ADP, and Pi has been cloned and sequenced. The mature protein, as deduced by the structural gene...

journal_title：Biochemistry

authors： Schendel FJ,Mueller E,Stubbe J,Shiau A,Smith JM

更新日期：1989-03-21 00:00:00

abstract：:Five previously unknown hemorrhagic proteins, designated hemorrhagic toxins a,b,c,d, and e, were isolated from the venom of the western diamondback rattlesnake (Crotalus atrox). Molecular weights of hemorrhagic toxins a-e were determined to be 68 000, 24 000, 24 000, 24 000, and 25 700, respectively, by sodium dodecyl...

journal_title：Biochemistry

authors： Bjarnason JB,Tu AT

更新日期：1978-08-08 00:00:00

abstract：:We examined the effects of water-soluble polymers of various degrees of hydrophobicity on the folding and aggregation of proteins. The polymers we chose were polyethylene glycol (PEG) and UCON (1:1 copolymer of ethylene glycol and propylene glycol). The presence of additional methyl groups in UCON makes it more hydrop...

journal_title：Biochemistry

authors： Breydo L,Sales AE,Frege T,Howell MC,Zaslavsky BY,Uversky VN

更新日期：2015-05-19 00:00:00

abstract：:The class D β-lactamase OXA-143 has been described as an efficient penicillinase, oxacillinase, and carbapenemase. The D224A variant, known as OXA-231, was described in 2012 as exhibiting less activity toward imipenem and increased oxacillinase activity. Additionally, the P227S mutation was reported as a case of conve...

journal_title：Biochemistry

authors： Antunes VU,Llontop EE,Vasconcelos FNDC,López de Los Santos Y,Oliveira RJ,Lincopan N,Farah CS,Doucet N,Mittermaier A,Favaro DC

更新日期：2019-08-27 00:00:00

abstract：:Regulation of cellular response is an important mechanism for controlling cellular functions. The transmembrane signaling of the hormone receptors is regulated by GTP-binding proteins (GTPases) and their associated proteins. Our previous studies demonstrated that the bifunctional GTP-binding protein, G alpha h (transg...

journal_title：Biochemistry

authors： Baek KJ,Das T,Gray CD,Desai S,Hwang KC,Gacchui R,Ludwig M,Im MJ

更新日期：1996-02-27 00:00:00

abstract：:We have previously shown that both recombinant apo(a) and native Lp(a) inhibit the binding of Glu-plasminogen to fibrin surfaces [Fleury & Anglés-Cano (1991) Biochemistry 30, 7630-7638; Rouy et al. (1992) Biochemistry 31, 6332-6339]. The aim of the present study was to characterize the mechanism of this inhibition and...

journal_title：Biochemistry

authors： Hervio L,Durlach V,Girard-Globa A,Anglés-Cano E

更新日期：1995-10-17 00:00:00

abstract：:An endo-beta-galactosidase, which hydrolyzes the internal beta-galactosidic linkages of R----GlcNAc (or GalNAc) beta 1----3Gal beta 1----4GlcNAc (or Glc), was isolated from the culture supernatant of Diplococcus pneumoniae. The enzyme, named endo-beta-galactosidase DII, hydrolyzed linear N-acetyllactosamine repeating ...

journal_title：Biochemistry

authors： Fukuda MN

更新日期：1985-04-23 00:00:00

abstract：:The epidermal growth factor receptor (EGFR) is a transmembrane protein involved in cell signaling processes, and dysregulation of its activity often drives tumor growth. EGFR is a clinically validated tumor marker and target for antibodies and tyrosine kinase inhibitors. We demonstrate that a fusion protein of the nat...

journal_title：Biochemistry

authors： Feiner RC,Pennè I,Müller B,Müller KM

更新日期：2019-02-26 00:00:00

abstract：:Firefly luciferase is 80-90% inactivated within 3 h upon incubation with the adenine nucleotide analogue p-fluorosulfonylbenzoyl-5'-adenosine (FSBA). Although 4 mol of 14C-FSBA/mol of enzyme is irreversibly bound during inactivation, only 1 mol of 14C-FSBA appears to be specifically directed to an adenine nucleotide b...

journal_title：Biochemistry

authors： Lee Y,Esch FS,DeLuca MA

更新日期：1981-03-03 00:00:00

abstract：:The energy derived from optimized van der Waals interactions in closely packed, folded proteins has been proposed to be of similar energetic magnitude to hydrophobicity in stabilizing the native state. If packing is this energetically important, it should influence the evolution of protein core sequences. To test this...

journal_title：Biochemistry

authors： Chen J,Stites WE

更新日期：2001-12-18 00:00:00

abstract：:The conformation of two fragments derived from anti-poly(D-alanyl) antibodies, the divalent fragment (Fab')2 and the monovalent fragment Fab', was studied by small-angle X-ray scattering before and after interaction with the tetra-D-alanine amide hapten. More than 90% of the combining sites were occupied by the hapten...

journal_title：Biochemistry

authors： Pilz I,Kratky O,Licht A,Sela M

更新日期：1975-03-25 00:00:00

abstract：:Continuum methods were used to calculate the electrostatic contributions of charged and polar side chains to the overall stability of a small 41-residue helical protein, the peripheral subunit-binding domain. The results of these calculations suggest several residues that are destabilizing, relative to hydrophobic iso...

journal_title：Biochemistry

authors： Spector S,Wang M,Carp SA,Robblee J,Hendsch ZS,Fairman R,Tidor B,Raleigh DP

更新日期：2000-02-08 00:00:00

abstract：:FtmPT1 is a fungal indole prenyltransferase that catalyzes the reaction of tryptophan derivatives with dimethylallyl pyrophosphate to form various biologically active compounds. Herein, we describe detailed studies of FtmPT1 catalysis involving dimethylallyl pyrophosphate and Brevianamide F following the native pathwa...

journal_title：Biochemistry

authors： Pan LL,Yang Y,Merz KM Jr

更新日期：2014-09-30 00:00:00

abstract：:Posttranslational glycosylation is critical for biological function of many proteins, but its structural characterization is complicated by natural heterogeneity, multiple glycosylation sites, and different forms. Here, a top-down mass spectrometry (MS) characterization is applied to three constructs of the Fc segment...

journal_title：Biochemistry

authors： Fridriksson EK,Beavil A,Holowka D,Gould HJ,Baird B,McLafferty FW

更新日期：2000-03-28 00:00:00

abstract：:The Pf1 gene 5 protein forms a large helical nucleoprotein complex (Mr = 3.1 x 10(7)) with single-stranded viral DNA, from which a 32 amino acid sequence rich in alanine, proline, and glutamine residues can be removed from the C-terminus by limited proteolysis. Sharp resonances in the 1H NMR spectrum of the Pf1 nucleo...

journal_title：Biochemistry

authors： Davis KG,Plyte SE,Robertson SR,Cooper A,Kneale GG

更新日期：1995-01-10 00:00:00

abstract：:Properties of the interaction of bleomycin (Blm) and metallobleomycins [M = Zn, Cu(II), Fe(III), and HO(2)-Co(III)] with site-specific and nonspecific DNA oligomers, d(GGAAGCTTCC)(2) (I) and d(GGAAATTTCC)(2) (II), respectively, were investigated. With both 10-mers association constants increased in the series Blm A(2)...

journal_title：Biochemistry

authors： Li W,Zhao C,Xia C,Antholine WE,Petering DH

更新日期：2001-06-26 00:00:00