当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Calmodulin-specific monoclonal antibodies inhibit DNA replication in mammalian cells.

Calmodulin-specific monoclonal antibodies inhibit DNA replication in mammalian cells.

Abstract：

:The involvement of calmodulin in the proliferation of Chinese hamster embryo fibroblast cells has been studied with a specific monoclonal antibody to calmodulin. We observed that calmodulin levels increase 2-fold in the late G1 period in these cells, and this coincides with the increase in DNA polymerase alpha activity as the cells progress synchronously from a quiescent state in the G1 to the S phase. However, there is a concurrent 10-fold enhancement of thymidine kinase activity, which is tightly coupled to the entry of cells into the S phase. Incubation of permeabilized S-phase cells with calmodulin-specific murine monoclonal antibody resulted in a dose-dependent inhibition of DNA replication. This inhibitory effect of anti-calmodulin antibodies on DNA replication is completely reversed by the addition of exogenously purified calmodulin. These observations provide evidence for the involvement of calmodulin in DNA replication and, therefore, in cell proliferation during the S phase.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Reddy GP,Reed WC,Sheehan E,Sacks DB

doi

10.1021/bi00158a002

subject

Has Abstract

pub_date

1992-11-03 00:00:00

pages

10426-30

issue

43

eissn

0006-2960

issn

1520-4995

journal_volume

31

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Integration of the thylakoid membrane protein cytochrome b6 in the cytoplasmic membrane of Escherichia coli.

    abstract：:An overexpression system for spinach apocytochrome b(6) as a fusion protein to a maltose-binding protein in Escherichia coli was established using the expression vector pMalp2. The fusion of the cytochrome b(6) to the periplasmic maltose-binding protein directs the cytochrome on the Sec-dependent pathway. The cytochro...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi047422w

    authors： Króliczewski J,Hombek-Urban K,Szczepaniak A

    更新日期：2005-05-24 00:00:00

  • Dissection of porphyrin-induced conformational dynamics in the heme biosynthesis enzyme ferrochelatase.

    abstract：:Human ferrochelatase (EC 4.99.1.1) catalyzes the insertion ferrous iron into protoporphyrin IX as the last step in heme biosynthesis, an essential process to most organisms given the vast intracellular functions of heme. Even with multiple ferrochelatase structures available, the exact mechanism for iron insertion int...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi300704c

    authors： Asuru AP,An M,Busenlehner LS

    更新日期：2012-09-11 00:00:00

  • Medium-Throughput Screen of Microbially Produced Serotonin via a G-Protein-Coupled Receptor-Based Sensor.

    abstract：:Chemical biosensors, for which chemical detection triggers a fluorescent signal, have the potential to accelerate the screening of noncolorimetric chemicals produced by microbes, enabling the high-throughput engineering of enzymes and metabolic pathways. Here, we engineer a G-protein-coupled receptor (GPCR)-based sens...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.7b00605

    authors： Ehrenworth AM,Claiborne T,Peralta-Yahya P

    更新日期：2017-10-17 00:00:00

  • Calorimetric study of the interaction of lysozyme with aqueous 1-propanol.

    abstract：:The enthalpies of transfer of hen egg white lysozyme from water to aqueous solutions of 1-propanol were determined by isothermal flow calorimetry at 10, 17, 25, and 40 degrees C in 0.04 M, pH 2 glycine buffer. Alcohol concentrations up to 3.4 M were employed. Four regions in the dependence of the enthalpy of transfer ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00514a016

    authors： Sturtevant JM,Velicelebi G

    更新日期：1981-05-26 00:00:00

  • Electrostatic guidance of glycosyl cation migration along the reaction coordinate of uracil DNA glycosylase.

    abstract：:The DNA repair enzyme uracil DNA glycosylase has been crystallized with a cationic 1-aza-2'-deoxyribose-containing DNA that mimics the ultimate transition state of the reaction in which the water nucleophile attacks the anomeric center of the oxacarbenium ion-uracil anion reaction intermediate. Comparison with substra...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi035372+

    authors： Bianchet MA,Seiple LA,Jiang YL,Ichikawa Y,Amzel LM,Stivers JT

    更新日期：2003-11-04 00:00:00

  • On the identity of nuclear membrane and non-histone nuclear proteins.

    abstract：:The fate of plasma and nuclear membrane polypeptides in preparations of acidic chromosomal protein from chicken erythrocytes has been investigated. It is shown that detergent extraction procedures (Nonidet P-40, Triton X-100, and saponin), commonly employed in the preparation of acidic chromosomal protein, cannot be r...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00670a034

    authors： Jackson RC

    更新日期：1976-12-14 00:00:00

  • FTIR study of a nonclassical dT10*dA10-dT10 intramolecular triple helix.

    abstract：:Many early investigations on triple helices have been devoted to the study of the triplex formed by dT*dA-dT base triplets in which the third strand is oriented parallel to the dA strand. We now describe an intramolecular triple helix with dT*dA-dT base triplets in which the pyrimidine third strand is oriented antipar...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00045a023

    authors： Dagneaux C,Liquier J,Taillandier E

    更新日期：1995-11-14 00:00:00

  • Activation-induced subcellular redistribution of G alpha(s) is dependent upon its unique N-terminus.

    abstract：:The heterotrimeric G protein subunit, alpha(s), can move reversibly from plasma membranes to cytoplasm in response to activation by GPCRs or activating mutations. We examined the importance of the unique N-terminus of alpha(s) in this translocation in cultured cells. alpha(s) contains a single site for palmitoylation ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi025533u

    authors： Thiyagarajan MM,Bigras E,Van Tol HH,Hébert TE,Evanko DS,Wedegaertner PB

    更新日期：2002-07-30 00:00:00

  • Isolation and in vitro phosphorylation of sensory transduction components controlling anaerobic induction of light harvesting and reaction center gene expression in Rhodobacter capsulatus.

    abstract：:Anaerobic induction of light harvesting and reaction center gene expression involves two transacting factors termed RegA and RegB. Sequence and mutational analysis has indicated that RegA and RegB constitute cognate components of a prokaryotic sensory transduction cascade with RegB comprising a membrane-spanning senso...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00002a002

    authors： Inoue K,Kouadio JL,Mosley CS,Bauer CE

    更新日期：1995-01-17 00:00:00

  • Cloning and characterization of a highly reiterated 5.8-kilobase pair nucleolar EcoRI DNA fragment found in Novikoff hepatoma ascites cells.

    abstract：:The DNA of Novikoff hepatoma ascites cells was found to contain a 3.6-megadalton EcoRI restriction fragment, referred to as EcoRI fragment A (Parker et al., 1979). C0t analyses demonstrated an enrichment of fragment A sequences in Novikoff hepatoma genome relative to normal rat liver DNA. This fragment was cloned in l...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00507a017

    authors： Parker DL,Busch H,Rothblum LI

    更新日期：1981-02-17 00:00:00

  • Proteolytic mapping of human replication protein A: evidence for multiple structural domains and a conformational change upon interaction with single-stranded DNA.

    abstract：:Replication protein A (RPA) is multisubunit single-stranded DNA-binding protein required for multiple processes in DNA metabolism including DNA replication, DNA repair, and recombination. Human RPA is a stable complex of three subunits of 70, 32, and 14 kDa (RPA70, RPA32, and RPA14, respectively). We examined the stru...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9526995

    authors： Gomes XV,Henricksen LA,Wold MS

    更新日期：1996-04-30 00:00:00

  • Structure of botulinum neurotoxin type D light chain at 1.65 A resolution: repercussions for VAMP-2 substrate specificity.

    abstract：:The seven serotypes (A-G) of botulinum neurotoxins (BoNTs) function through their proteolytic cleavage of one of three proteins (SNAP-25, Syntaxin, and VAMP) that form the SNARE complex required for synaptic vesicle fusion. The different BoNTs have very specific protease recognition requirements, between 15 and 50 ami...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi052518r

    authors： Arndt JW,Chai Q,Christian T,Stevens RC

    更新日期：2006-03-14 00:00:00

  • Isolation of seventeen proteins and amino-terminal amino acid sequences of eight proteins from cytoplasmic ribosomes of yeast.

    abstract：:Seventeen ribosomal proteins of Saccharomyces cerevisiae were isolated from small ribosomal subunits and disodium ethylenediaminetetraacetate treated 80S ribosomes by chromatography on a column of carboxymethylcellulose and/or by filtration through Sephacryl S-200. The isolated proteins are YS4, YS7, YS8, YS9, YS10, Y...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00262a005

    authors： Otaka E,Higo K,Osawa S

    更新日期：1982-09-14 00:00:00

  • Restriction of the lateral motion of band 3 in the erythrocyte membrane by the cytoskeletal network: dependence on spectrin association state.

    abstract：:The effects of incubation of erythrocyte ghosts under various conditions (ionic strength or addition of ankyrin, diamines, or ATP) on the lateral motion of band 3 in the membranes were studied by using the fluorescence photobleaching recovery technique. Incubation of ghosts with exogenous ankyrin increased the immobil...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00368a045

    authors： Tsuji A,Ohnishi S

    更新日期：1986-10-07 00:00:00

  • Ribosome subunit to polysome ratios affect the synthesis of rRNA in Drosophila cells.

    abstract：:Many investigations have revealed that ribosome numbers increase in parallel with the growth rate of cells. Here we show that the absolute level of protein synthesis may not be the only factor influencing rRNA synthesis in a nondividing eukaryotic cell. Under conditions of complete (greater than 99%) inhibition of pro...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00502a003

    authors： Yamamoto KK,Pellegrini M

    更新日期：1990-12-18 00:00:00

  • Mn2+ is a native metal ion activator for bacteriophage lambda protein phosphatase.

    abstract：:Bacteriophage lambda protein phosphatase (lambdaPP) is a member of a large family of metal-containing phosphoesterases, including purple acid phosphatase, protein serine/threonine phosphatases, 5'-nucleotidase, and DNA repair enzymes such as Mre11. lambdaPP can be activated several-fold by various divalent metal ions,...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi026317o

    authors： Reiter TA,Reiter NJ,Rusnak F

    更新日期：2002-12-24 00:00:00

  • Two unique phosphorylation-driven signaling pathways crosstalk in Staphylococcus aureus to modulate the cell-wall charge: Stk1/Stp1 meets GraSR.

    abstract：:The Stk1/Stp1 and GraSR signal-transduction pathways are two distinct pathways in Staphylococcus aureus that rely on a reversible phosphorylation process in transducing external stimuli intracellularly. Stk1/Stp1 is an eukaryote-like Ser/Thr kinase phosphatase pair involved in purine biosynthesis, cell-wall metabolism...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi401177n

    authors： Fridman M,Williams GD,Muzamal U,Hunter H,Siu KW,Golemi-Kotra D

    更新日期：2013-11-12 00:00:00

  • Differential coupling of smooth and skeletal muscle pyruvate kinase to creatine kinase.

    abstract：:The interaction of pyruvate kinase from skeletal (SKPK) and smooth (SMPK) muscle with MM-creatine kinase (MMCK) and BB-creatine kinase (BBCK) was assessed using temporal absorbance changes, variations in absorbance at different wavelengths, concentration dependence, association in an electric field, and PK kinetic act...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi991076e

    authors： Sears PR,Dillon PF

    更新日期：1999-11-09 00:00:00

  • Pre-steady-state kinetic analysis of enzyme-monitored turnover during cystathionine β-synthase-catalyzed H(2)S generation.

    abstract：:Cystathionine β-synthase (CBS) catalyzes the first step in the transsulfuration pathway in mammals, i.e., the condensation of serine and homocysteine to produce cystathionine and water. Recently, we have reported a steady-state kinetic analysis of the three hydrogen sulfide (H(2)S)-generating reactions that are cataly...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi1010893

    authors： Singh S,Ballou DP,Banerjee R

    更新日期：2011-01-25 00:00:00

  • Disulfide bond formation between the active-site thiol and one of the several free thiol groups of chymopapain.

    abstract：:Chymopapain, a cysteine protease of papaya latex, has been purified with the use of fast protein liquid chromatography. Two homogeneous fractions were analyzed for thiol content and thiol reactivity. It was found that peak 1 and peak 2 contained two and three thiol groups, respectively, per mole of enzyme. This result...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00370a024

    authors： Kóródi I,Asbóth B,Polgár L

    更新日期：1986-11-04 00:00:00

  • Identification of peptide antagonists to glycoprotein Ibalpha that selectively inhibit von Willebrand factor dependent platelet aggregation.

    abstract：:GPIbalpha is an integral membrane protein of the GPIb-IX-V complex found on the platelet surface that interacts with the A1 domain of von Willebrand factor (vWF-A1). The interaction of GPIbalpha with vWF-A1 under conditions of high shear stress is the first step in platelet-driven thrombus formation. Phage display was...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi702428q

    authors： Benard SA,Smith TM,Cunningham K,Jacob J,DeSilva T,Lin L,Shaw GD,Kriz R,Kelleher KS

    更新日期：2008-04-22 00:00:00

  • Aggregation studies on fluorescein-coupled cobra venom phospholipase A2.

    abstract：:Phospholipase A2 from Naja naja naja venom (Indian cobra) undergoes a concentration-dependent aggregation, and at an assay concentration of 1 microgram mL-1, it exists as a monomer. However, there is some evidence that the enzyme is actually active as a dimer or higher order aggregate. Previous attempts to determine t...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00343a018

    authors： Hazlett TL,Dennis EA

    更新日期：1985-10-22 00:00:00

  • Role of arginine-292 in the substrate specificity of aspartate aminotransferase as examined by site-directed mutagenesis.

    abstract：:X-ray crystallographic data have implicated Arg-292 as the residue responsible for the preferred side-chain substrate specificity of aspartate aminotransferase. It forms a salt bridge with the beta or gamma carboxylate group of the substrate [Kirsch, J. F., Eichele, G., Ford, G. C., Vincent, M. G., Jansonius, J. N., G...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00412a052

    authors： Cronin CN,Kirsch JF

    更新日期：1988-06-14 00:00:00

  • Role of arginine residue in saccharopine dehydrogenase (L-lysine forming) from baker's yeast.

    abstract：:The baker's yeast saccharopine dehydrogenase (EC 1.5.1.7) was inactivated by 2,3-butanedione following pseudo-first-order reaction kinetics. The pseudo-first-order rate constant for inactivation was linearly related to the butanedione concentration, and a value of 7.5 M-1 min-1 was obtained for the second-order rate c...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00506a004

    authors： Fujioka M,Takata Y

    更新日期：1981-02-03 00:00:00

  • Stereochemical outcome and kinetic effects of Rp- and Sp-phosphorothioate substitutions at the cleavage site of vaccinia type I DNA topoisomerase.

    abstract：:To probe the mechanism of the reversible DNA phosphodiester bond cleavage and religation mechanism of the type I topoisomerase from vaccinia virus, we have synthesized DNA substrates carrying a single nonbridging Rp- or Sp-phosphorothioate (Ps) modification at the scissile phosphodiester (Pd) bond. Analysis of the ste...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi992429c

    authors： Stivers JT,Jagadeesh GJ,Nawrot B,Stec WJ,Shuman S

    更新日期：2000-05-09 00:00:00

  • Dynamics and binding mode of Hoechst 33258 to d(GTGGAATTCCAC)2 in the 1:1 solution complex as determined by two-dimensional 1H NMR.

    abstract：:We have investigated the interaction of the bisbenzimidazole derivative Hoechst 33258 with the self-complementary dodecadeoxynucleotide duplex d(GTGGAATTCCAC)2 using one-dimensional (1D) and two-dimensional (2D) proton nuclear magnetic resonance (1H NMR) spectroscopy. To monitor the extent of complex formation, we use...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00112a004

    authors： Fede A,Labhardt A,Bannwarth W,Leupin W

    更新日期：1991-12-03 00:00:00

  • Cryoenzymic studies on actomyosin ATPase. Evidence that the degree of saturation of actin with myosin subfragment 1 affects the kinetics of the binding of ATP.

    abstract：:The initial steps of actomyosin subfragment 1 (acto-S1) ATPase (dissociation and binding of ATP) were studied at -15 degrees C with 40% ethylene glycol as antifreeze. The dissociation kinetics were followed by light scattering in a stopped-flow apparatus, and the binding of ATP was followed by the ATP chase method in ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00459a026

    authors： Tesi C,Travers F,Barman T

    更新日期：1990-02-20 00:00:00

  • Inhibition of c-src transcription by mithramycin: structure-activity relationships of biosynthetically produced mithramycin analogues using the c-src promoter as target.

    abstract：:The aureolic acid antitumor antibiotic mithramycin (MTM) inhibits both cancer growth and bone resorption by cross-linking GC-rich DNA, thus blocking binding of Sp-family transcription factors to gene regulatory elements. Transcription of c-src, a gene implicated in many human cancers and required for osteoclast-depend...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi034091z

    authors： Remsing LL,Bahadori HR,Carbone GM,McGuffie EM,Catapano CV,Rohr J

    更新日期：2003-07-15 00:00:00

  • Truncation of the amino terminus of human apolipoprotein A-I substantially alters only the lipid-free conformation.

    abstract：:An amino-terminal deletion mutant (residues 1-43) of human apolipoprotein A-I (apo hA-I) has been produced from a bacterial expression system to explore the structural and functional role of these amino acids, encoded by exon 3, in apo hA-I. Lipid binding of apo delta (1-43)A-I and lipid binding of apo hA-I are very s...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi961876e

    authors： Rogers DP,Brouillette CG,Engler JA,Tendian SW,Roberts L,Mishra VK,Anantharamaiah GM,Lund-Katz S,Phillips MC,Ray MJ

    更新日期：1997-01-14 00:00:00

  • Structural basis and binding properties of the second bromodomain of Brd4 with acetylated histone tails.

    abstract：:Brd4 belongs to the BET family. It is a multifunctional protein involved in transcription, replication, the signal transduction pathway, and cell cycle progression. All of these functions are linked to its association with acetylated chromatin. With its tandem bromodomains, Brd4 avidly binds to diacetylated H4-AcK5/K1...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi8001659

    authors： Liu Y,Wang X,Zhang J,Huang H,Ding B,Wu J,Shi Y

    更新日期：2008-06-17 00:00:00