Abstract:
:The interaction of pyruvate kinase from skeletal (SKPK) and smooth (SMPK) muscle with MM-creatine kinase (MMCK) and BB-creatine kinase (BBCK) was assessed using temporal absorbance changes, variations in absorbance at different wavelengths, concentration dependence, association in an electric field, and PK kinetic activity. SKPK exhibits a time course of absorbance increase in the presence of MMCK with a time constant of 29.5 min. This increase occurs at all wavelength from 240 to 1000 nm. At 195 nm, the combination of SKPK and MMCK produces a decrease in absorption with electric fields of both 0 and 204 V/cm. The change in SKPK-MMCK is saturable. SKPK activity is significantly increased by the presence of MMCK in solutions of 0-32% ethanol. These results indicate specific SKPK-MMCK interaction. SMPK and BBCK did not exhibit similar coupling when the BBCK concentration dependence of absorbance or SMPK activity in solutions of 0-32% ethanol was determined. Both MMCK and BBCK increased SKPK activity; neither MMCK nor BBCK increased SMPK activity. The ability to form diazymatic complexes with creatine kinase appears to reside in SKPK. This coupling may account for the increased flux through PK without significant substrate changes seen during skeletal muscle activation. This coupling will not occur in smooth muscle.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Sears PR,Dillon PFdoi
10.1021/bi991076esubject
Has Abstractpub_date
1999-11-09 00:00:00pages
14881-6issue
45eissn
0006-2960issn
1520-4995pii
bi991076ejournal_volume
38pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Dodecameric glutamine synthetase (GS) from bacteria is formed from two face-to-face hexameric rings of identical subunits. These highly symmetrical aggregates from some bacteria, including Escherichia coli, "stack" in the presence of Zn2+ and other divalent ions to generate protein tubes (phase I) and subsequently ass...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00254a001
更新日期:1994-12-20 00:00:00
abstract::The physiological effects of anesthetics have been ascribed to their interaction with hydrophobic sites within functionally relevant CNS proteins. Studies have shown that volatile anesthetics compete for luciferin binding to the hydrophobic substrate binding site within firefly luciferase and inhibit its activity (Fra...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi800544d
更新日期:2008-09-09 00:00:00
abstract::Sequential 1H NMR assignments of a metallothionein from Neurospora crassa have been accomplished by the combined use of COSY, 2QF-COSY, HOHAHA, and rotating-frame NOE experiments. All potentially observable resonances were assigned except for the epsilon-NH3 group of the C-terminal lysine. 1H NOEs, when observed in th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00433a038
更新日期:1989-04-04 00:00:00
abstract::The effects of several guanine nucleotide analogues on (Na+,K+)-ATPase activity of membranes isolated from several tissues were analyzed to determine if a G-protein might be involved in the hormonal regulation of the (Na+,K+)-ATPase. Submillimolar concentrations of GTP gamma S, but not GMPPNP, inhibit rat skeletal mus...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00241a014
更新日期:1991-07-09 00:00:00
abstract::Cyanobacteriochromes (CBCRs) are photosensory proteins related to the red/far-red phytochromes. Like phytochromes, CBCRs use linear tetrapyrrole (bilin) chromophores covalently attached via a thioether linkage to a conserved Cys residue also found in plant and cyanobacterial phytochromes. Unlike almost all phytochrome...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300171s
更新日期:2012-05-01 00:00:00
abstract::We determined the effect of vertebrate albumins on the stability of several physiologically relevant prostaglandins. All naturally occurring prostaglandins with beta-hydroxy ketone group decomposed by first-order kinetics, dependent on the albumin concentration in 0.1 M, pH 7.4, buffer at 37 degrees C. Even subphysiol...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00524a033
更新日期:1981-10-13 00:00:00
abstract::One-dimensional 1H NMR was employed to study the effects of Ca2+ and Lu3+ binding on the apo and calcium-saturated forms of dog bone Gla protein (BGP, osteocalcin). Titration of apo dog BGP with Ca2+ in 20 mM NaCl showed spectral perturbations consistent with the binding of 5 mol equiv of calcium in the NMR slow-excha...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00093a012
更新日期:1993-10-26 00:00:00
abstract::Recombinant human 3'-phosphoadenosine 5'-phosphosulfate (PAPS) synthetase, isoform 1 (brain), was purified to near-homogeneity from an Escherichia coli expression system and kinetically characterized. The native enzyme, a dimer with each 71 kDa subunit containing an adenosine triphosphate (ATP) sulfurylase and an aden...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi049827m
更新日期:2004-04-13 00:00:00
abstract::Small molecule allostery modifies protein function but is not easily discovered. We introduce mass spectrometry integrated with equilibrium dialysis for the discovery of allostery systematically (MIDAS), a method for identifying physiologically relevant, low-affinity metabolite-protein interactions using unmodified pr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi201313s
更新日期:2012-01-10 00:00:00
abstract::Indolicidin is an antimicrobial cationic peptide with broad-spectrum activity isolated from bovine neutrophils. An indolicidin analogue CP-11, ILKKWPWWPWRRK-NH(2), with improved activity against Gram-negative bacteria had increased positive charge and amphipathicity while maintaining the short length of the parent mol...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi035643g
更新日期:2003-12-09 00:00:00
abstract::The pentraxin serum amyloid P component (SAP) is secreted by the liver and found in plasma at a concentration of approximately 30 mg/L. SAP is a 25 kDa homopentamer known to bind both protein and nonprotein ligands, all in a calcium-dependent manner. The function of SAP is unclear but likely involves the humoral innat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b01027
更新日期:2017-02-14 00:00:00
abstract::The quantitative tissue specificity of the high mobility group (HMG) chromosomal proteins was investigated. Perchloric acid (PCA) extracts of four different chicken tissues and erythrocytes contained three proteins which comigrated on NaDodSO4-polyacrylamide gels with the HMG's 1,2, and E from erythrocyte nuclei. Thes...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00560a003
更新日期:1980-09-16 00:00:00
abstract::Amyloid diseases make up a set of fatal disorders in which proteins aggregate to form fibrils that deposit in tissues throughout the body. Amyloid-associated diseases are challenging to study because amyloid formation occurs on time scales that span several orders of magnitude and involve heterogeneous, interconvertin...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.9b00769
更新日期:2019-11-05 00:00:00
abstract::Electron transfer between the Rieske iron-sulfur protein (Fe(2)S(2)) and cytochrome c(1) was studied using the ruthenium dimer, Ru(2)D, to either photoreduce or photooxidize cytochrome c(1) within 1 micros. Ru(2)D has a charge of +4, which allows it to bind with high affinity to the cytochrome bc(1) complex. Flash pho...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi000003o
更新日期:2000-04-18 00:00:00
abstract::The number of free cysteines in each polypeptide of acetylcholine receptor from the electric organ of Torpedo californica has been assessed by alkylating the native protein with N-ethylmaleimide and iodoacetamide during homogenization of the tissue and alkylating the polypeptides with N-ethylmaleimide as they were unf...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00434a048
更新日期:1989-04-18 00:00:00
abstract::A preparation of small single-walled liposome vesicles containing a 9:1 mole ratio of phosphatidylcholine to phosphatidylethanolamine was subjected to reaction with the membrane-impermeable reagent, isethionyl acetimidate hydrochloride. This reagent converted 90% of the external phosphatidylethanolamine groups to the ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00693a008
更新日期:1975-11-04 00:00:00
abstract::Acarbose is a naturally occurring pseudo-tetrasaccharide. It has been used in conjunction with other drugs in the treatment of diabetes where it acts as an inhibitor of intestinal glucosidases. To probe the interactions of acarbose with other carbohydrate recognition enzymes, the crystal structure of E. coli maltodext...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9828573
更新日期:1999-04-27 00:00:00
abstract::Atomic resolution ( journal_title:Biochemistry pub_type: 杂志文章 doi:10.1021/bi0517133 更新日期:2006-02-21 00:00:00
abstract::IIIGlc is an 18.1-kDa signal-transducing phosphocarrier protein of the phosphoenolpyruvate:glycose phosphotransferase system (PTS) of Escherichia coli. Virtually complete (98%) backbone 1H, 15N, and 13C nuclear magnetic resonance (NMR) signal assignments were determined by using a battery of triple-resonance three-dim...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00105a032
更新日期:1991-10-15 00:00:00
abstract::Amphiphiles respond both to polar and to nonpolar solvents. In this paper X-ray diffraction and osmotic stress have been used to examine the phase behavior, the structural dimensions, and the work of deforming the monolayer-lined aqueous cavities formed by mixtures of dioleoylphosphatidylethanolamine (DOPE) and dioleo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00453a010
更新日期:1990-01-09 00:00:00
abstract::The bovine protein tyrosine phosphatase (BPTP) is a member of the class of low-molecular weight protein tyrosine phosphatases (PTPases) found to be ubiquitous in mammalian cells. The catalytic site of BPTP contains a CX(5)R(S/T) phosphate-binding motif or P-loop (residues 12-19) which is the signature sequence for all...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi990381x
更新日期:1999-09-07 00:00:00
abstract::The third and most recently identified Parkinson's disease-linked variant of the neuronal protein alpha-synuclein to be identified (E46K) results in widespread brain pathology and early onset Parkinson symptoms (Zarranz et al. (2004) Ann. Neurol. 55, 164-173). Herein, we present biochemical and biophysical characteriz...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi7000246
更新日期:2007-06-19 00:00:00
abstract::Fluid-phase interactions between hematologic cells and those of the vessel wall were studied in order to define a role for lipoxygenase products as cell signals in the control of vascular cholesterol metabolism. A functional parameter for hydroxy acids in this system has not been previously demonstrated. We report her...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00448a030
更新日期:1989-10-31 00:00:00
abstract::Alzheimer's disease (AD) paired helical filaments (PHFs), building blocks of neurofibrillary tangles (NFTs) are composed of hyperphosphorylated forms of the microtubule-associated protein tau (i.e., PHF-tau). Currently, much effort is devoted to the development of diagnostic antibodies specific for PHF-tau since eleva...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi970380+
更新日期:1997-07-01 00:00:00
abstract::The crystal structure of a human carbonic anhydrase II (CAII) variant, cis-proline-202-->alanine (P202A), has been determined at 1.7-A resolution, indicating that the wild-type geometry, including the cis-peptidyl linkage, is retained upon substitution of proline by alanine. The CO2 hydrase activity and affinity for s...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00092a003
更新日期:1993-10-19 00:00:00
abstract::Phoborhodopsin from Halobacterium salinarum (salinarum phoborhodopsin, spR also called HsSR II) is a photoreceptor for the negative phototaxis of the bacterium. A unique feature of spR is the formation of a shorter wavelength photoproduct, P480, observed at liquid nitrogen temperature beside the K intermediate. Format...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200598r
更新日期:2011-08-23 00:00:00
abstract::The alpha, beta, and gamma subunits of the receptor with high affinity for immunoglobulin E were isolated and their compositions assessed by direct amino acid analysis and by incorporation of radioactive precursors. The compositions show no unusual features other than a rather high content of tryptophan in the alpha c...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00383a024
更新日期:1987-05-05 00:00:00
abstract::Many strains of Campylobacter jejuni display modified heptose residues in their capsular polysaccharides (CPS). The precursor heptose was previously shown to be GDP-d-glycero-α-d-manno-heptose, from which a variety of modifications of the sugar moiety have been observed. These modifications include the generation of 6...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.0c00096
更新日期:2020-04-07 00:00:00
abstract::P-Glycoprotein (Pgp) expression in cell lines derived from tumors arising from cells which normally express Pgp can be increased by sodium butyrate and other differentiating agents. Although the Pgp level increased 25-fold after sodium butyrate treatment in SW620 human colon carcinoma cells, the intracellular accumula...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00143a002
更新日期:1992-07-21 00:00:00
abstract::Activation of a growth factor receptor tyrosine kinase (RTK) is accompanied by a rapid autophosphorylation of the receptor on tyrosine residues. Receptor activation has been shown to promote the association of signal-transducing proteins containing SH2 domains (second domain of src homology). These receptor-associated...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00089a028
更新日期:1993-09-28 00:00:00