Abstract:
:The bovine protein tyrosine phosphatase (BPTP) is a member of the class of low-molecular weight protein tyrosine phosphatases (PTPases) found to be ubiquitous in mammalian cells. The catalytic site of BPTP contains a CX(5)R(S/T) phosphate-binding motif or P-loop (residues 12-19) which is the signature sequence for all PTPases. Ser19, the final residue of the P-loop motif, interacts with the catalytic Cys12 and participates in stabilizing the conformation of the active site through interactions with Asn15, also in the P-loop. Mutations at Ser19 result in an enzyme with altered kinetic properties with changes in the pK(a) of the neighboring His72. The X-ray structure of the S19A mutant enzyme shows that the general conformation of the P-loop is preserved. However, changes in the loop containing His72 result in a displacement of the His72 side chain that may explain the shift in the pK(a). In addition, it was found that in the crystal, the protein forms a dimer in which Tyr131 and Tyr132 from one monomer insert into the active site of the other monomer, suggesting a dual-tyrosine motif on target sites for this enzyme. Since the activity of this PTPase is reportedly regulated by phosphorylation at Tyr131 and Tyr132, the structure of this dimer may provide a model of a self-regulation mechanism for the low-molecular weight PTPases.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Tabernero L,Evans BN,Tishmack PA,Van Etten RL,Stauffacher CVdoi
10.1021/bi990381xsubject
Has Abstractpub_date
1999-09-07 00:00:00pages
11651-8issue
36eissn
0006-2960issn
1520-4995pii
bi990381xjournal_volume
38pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Conformational factors that predicate selectivity for valine or isoleucine binding to IlvN leading to the regulation of aceto hydroxy acid synthase I (AHAS I) of Escherichia coli have been determined for the first time from high-resolution (1.9-2.43 Å) crystal structures of IlvN·Val and IlvN·Ile complexes. The valine ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.9b00050
更新日期:2019-04-16 00:00:00
abstract::Two paramagnetic probes, viz., Mn2+ and Cr3+-ATP, were used to map distances to various loci on carbamoyl-phosphate synthetase by using NMR measurements. The paramagnetic influence of Mn2+ on the 1H of L-glutamate and L-ornithine was measured at 200 and 360 MHz. On the basis of these data, a correlation time for the p...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00277a020
更新日期:1983-04-12 00:00:00
abstract::Hirudin is the most potent and specific thrombin inhibitor from medicinal leech with a Ki value of 2.2 x 10(-14) M. It consists of an active site inhibitor segment, hirudin1-48, a fibrinogen-recognition exosite inhibitor segment, hirudin55-65, and a linker, hirudin49-54, connecting these inhibitor segments. The role o...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00027a021
更新日期:1995-07-11 00:00:00
abstract::The covalent affinity probe maltosyl isothiocyanate (MITC) has been used previously to identify the glucose transporter of human erythrocytes as a component of band 3. By use of limited proteolysis, the site on the Mr 100 000 protein to which MITC attaches has been localized to a 17 000-dalton region near the center o...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00331a001
更新日期:1985-05-07 00:00:00
abstract::Thermodynamic parameters of helix formation were measured spectroscopically for 16 oligoribonucleotides containing either internal GU mismatches or the corresponding AU pairs. Internal GU mismatches stabilize each helix, but not as much as the corresponding AU pairs. The differences in the enthalpy and entropy changes...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00367a061
更新日期:1986-09-23 00:00:00
abstract::Recent studies of proteins with reversed charged residues have demonstrated that electrostatic interactions on the surface can contribute significantly to protein stability. We have used the approach of reversing negatively charged residues using Arg to evaluate the effect of the electrostatics context on the transiti...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0354684
更新日期:2004-04-06 00:00:00
abstract::Protein phosphatase-1 (PP1) is an essential protein Ser/Thr phosphatase that is extraordinarily conserved from yeast to human, and Inhibitor-2 (I-2) is the most ancient of the heat-stable proteins specific for PP1. We identified novel I-2 homologues in Caenorhabditis elegans (Ce) and Xenopus laevis (Xe) and compared t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi602369m
更新日期:2007-03-06 00:00:00
abstract::Neuropeptide Y (NPY) and the pancreatic polypeptide (PP) are members of the neuropeptide Y family of hormones. They bind to the Y receptors with very different affinities: Whereas PP is highly selective for the Y(4) receptor, NPY displays highest affinites for Y(1), Y(2), and Y(5) receptor subtypes. Introducing the NP...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0501232
更新日期:2005-06-28 00:00:00
abstract::Frequency-resolved fluorescence measurements have been performed to quantitate the lateral stress of the lipid layer containing nonbilayer phase preferring dioleoylphosphatidylethanolamine (DOPE). On the basis of a new rotational diffusion model, the wobbling diffusion constant (Dw), the curvature-related hopping diff...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00130a005
更新日期:1992-04-21 00:00:00
abstract::Protein misfolding and aggregation is a ubiquitous phenomenon associated with a wide range of diseases. The synuclein family comprises three small naturally unfolded proteins implicated in neurodegenerative diseases and some forms of cancer. α-Synuclein is a soluble protein that forms toxic inclusions associated with ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300478w
更新日期:2012-06-12 00:00:00
abstract::The idea of establishing the amyloid-like fibrillation tendency of pro- and antisurvival proteins of human apoptotic pathways is relevant for delineating the conditions that lead to aberrant differentiation, development, and tissue homeostasis. As the first step in this direction, we report here that the caspase recru...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi900626u
更新日期:2009-08-18 00:00:00
abstract::A series of sulfonate esters that are attached to a noncationic minor-groove-binding N-methylpyrrole dipeptide (Lex) related to netrospin have been synthesized. The compounds prepared differ in two respects: (1) the length [(CH2)2 vs (CH2)8] of the tether between the DNA affinity binding portion of the molecule and th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00082a017
更新日期:1993-08-10 00:00:00
abstract::The Bacillus subtilis DNA-dependent RNA polymerase holoenzyme and core enzyme each contain approximately two atoms of zinc per molecule. When the dissociated subunits of the enzyme are passed through a blue dextran-Sepharose affinity column, only the beta subunit binds to the column. The total zinc content of the enzy...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00632a012
更新日期:1977-06-28 00:00:00
abstract::Integrins are one of the major mediators of cellular adherence. Structurally the component alpha and beta chains are characterized by extensive intrachain disulfide bonding. The assignment of these bonds is currently based on homology with the chains of the integrin alphaIIbbeta3. However, recent crystallographic anal...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034726u
更新日期:2003-11-11 00:00:00
abstract::Electron-transfer reactions following the formation of P700(+)A1- have been studied in isolated Photosystem I complexes from Synechococcus elongatus between 300 and 5 K by flash absorption spectroscopy. (1) In the range from 300 to 200 K, A1- is reoxidized by electron transfer to the iron-sulfur cluster FX. The rate s...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi973182r
更新日期:1998-06-30 00:00:00
abstract::The kinetics of ATP-induced Ca2+ uptake by vesicular dispersions of sarcoplasmic reticulum were determined with a time resolution of about 10 ms, depending on the temperature. Ca2+ uptake was initiated by the addition of ATP through the flash photolysis of P3-1-(2-nitrophenyl)-ethyl adenosine 5'-triphosphate utilizing...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00292a003
更新日期:1983-11-08 00:00:00
abstract::We present spectroscopic evidence for the presence of low-barrier hydrogen bonds (LBHBs) in molecular complexes composed of carboxylic acids and 1-methylimidazole (1-MeIm) dissolved in aprotic organic solvents. A plot of the values of the low-field proton NMR chemical shifts versus the aqueous pKa of the carboxylic ac...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00021a002
更新日期:1995-05-30 00:00:00
abstract::Many naturally occurring RNA structures contain single mismatches. However, the algorithms currently used to predict RNA structure from sequence rely on a minimal set of data for single mismatches, most of which occur rather infrequently in nature. As a result, several approximations and assumptions are used to predic...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi701311c
更新日期:2007-11-20 00:00:00
abstract::New homo- and heterobifunctional cross-linking reagents have been synthesized. These reagents are based on ortho ester, acetal, and ketal functionalities that undergo acid-catalyzed dissociation but are base stable. The protein-reactive group in all the homobifunctional reagents is a maleimide group; the heterobifunct...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00432a023
更新日期:1989-03-21 00:00:00
abstract::A simple model system is described that allows measurement of equilibrium Ca2+ binding to multilamellar vesicle mixtures of palmitoyloleoylphosphatidylserine (P,O-PS) and dimyristoleoylphosphatidylcholine (MO,MO-PC). The constraint of the chemical equilibrium among aqueous Ca2+, hydrated P,O-PS/MO,MO-PC, and Ca(PS)2, ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00488a013
更新日期:1990-09-11 00:00:00
abstract::Compartmentation and NMR visibility of mitochondrial adenine nucleotides were quantitated in isolated rat liver mitochondria respiring on succinate and glutamate in vitro at 8 and 25 degrees C. Intra- and extramitochondrial nucleotides were discriminated by adding the chelator trans-1,2-diaminocyclohexane-N,N,N',N'-te...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00436a030
更新日期:1989-05-16 00:00:00
abstract::Proteins from the enhanced intracellular survival (Eis) family are versatile acetyltransferases that acetylate amines at multiple positions of several aminoglycosides (AGs). Their upregulation confers drug resistance. Homologues of Eis are present in diverse bacteria, including many pathogens. Eis from Mycobacterium t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b00244
更新日期:2015-05-26 00:00:00
abstract::Membrane vesicles from Mycobacterium phlei contain carrier proteins for proline, glutamine, and glutamic acid. The transport of proline is Na+ dependent and required substrate oxidation. A proline carrier protein was solubilized from the membrane vesicles by treatment with cholate and Triton X-100. Electron microscopi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00578a015
更新日期:1979-05-29 00:00:00
abstract::The functional role of distinct phospholipid subclasses and molecular species in modulating gramicidin-mediated K+ flux was characterized through quantification of changes in the fluorescence intensity of ion specific fluorescent probes encapsulated inside vesicles comprised of individual molecular species of plasmeny...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00022a008
更新日期:1995-06-06 00:00:00
abstract::ArsR (or ArsR/SmtB) family metalloregulatory homodimeric repressors collectively respond to a wide range of metal ion inducers in regulating homeostasis and resistance of essential and nonessential metal ions in bacteria. BxmR from the cyanobacterium Osciliatoria brevis is the first characterized ArsR protein that sen...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi801313y
更新日期:2008-10-07 00:00:00
abstract::Flash-induced Fourier transform infrared (FTIR) difference spectra for the four-step S-state cycle and the effects of global (15)N- and (13)C-isotope labeling on the difference spectra were examined for the first time in the mid- to low-frequency (1200-800 cm(-1)) as well as the mid-frequency (1700-1200 cm(-1)) region...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0362323
更新日期:2004-06-15 00:00:00
abstract::Hydrolysis of monodisperse short chain phosphatidylcholines, far below their critical micelle concentration, by phospholipase A2 (PLA2) and other interfacial enzymes is characterized. Results show that virtually all the observed hydrolysis by pancreatic and human inflammatory PLA2 occurs on surfaces of the reaction ve...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi990194z
更新日期:1999-08-10 00:00:00
abstract::The enzyme phosphoenolpyruvate carboxykinase (PEPCK) catalyzes the reversible conversion of oxalacetate and GTP to phosphoenolpyruvate (PEP), GDP, and CO2. PEPCK from higher organisms is a monomer, specifically requires GTP or ITP, and uses Mn2+ as the activating cation. Currently, there is no crystal structure of GTP...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi011374n
更新日期:2001-09-18 00:00:00
abstract::The following polynucleotides containing the antibiotic tubercidin (Tu; 4-amino-7-beta-D-ribofuranosylpyrrolo-[2,3-d]pyrimidine) were enzymatically synthesized by polymerization of adenosine 5'-diphosphate-tubercidin 5'-diphosphate mixtures with polynucleotide phosphorylase: poly(A2,Tu), poly(A,Tu2), and poly(Tu). The...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00512a030
更新日期:1981-04-28 00:00:00
abstract::Cell-signaling peptides have been extensively used to transport functional molecules across the plasma membrane into living cells. These peptides consist of a hydrophobic sequence and a cationic nuclear localization sequence (NLS). It has been assumed that the hydrophobic region penetrates the hydrophobic lipid bilaye...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061895g
更新日期:2007-01-30 00:00:00