Interactions of hirudin-based inhibitor with thrombin: critical role of the IleH59 side chain of the inhibitor.

abstract：:The aldehyde oxidoreductase from Desulfovibrio gigas belongs to the family of molybdenum hydroxylases. Besides a molybdenum cofactor which constitutes their active site, these enzymes contain two [2Fe-2S](2+,1+) clusters which are believed to transfer the electrons provided by the substrate to an acceptor which is eit...

journal_title：Biochemistry

authors： More C,Asso M,Roger G,Guigliarelli B,Caldeira J,Moura J,Bertrand P

更新日期：2005-08-30 00:00:00

abstract：:The kinetics of folding/unfolding of cross-linked Lys7-dinitrophenylene-Lys41-ribonuclease A were studied and compared to those of unmodified ribonuclease A (RNase A) at various concentrations of guanidine hydrochloride. The folding of the denatured cross-linked protein involved one fast-folding species (22 +/- 4%) an...

journal_title：Biochemistry

authors： Lin SH,Konishi Y,Nall BT,Scheraga HA

更新日期：1985-05-21 00:00:00

abstract：:Although plasma membrane (PM) cholesterol-rich and -poor domains have been isolated by subcellular fractionation, the real-time arrangement of cholesterol in such domains in living cells is still unclear. Therefore, dehydroergosterol (DHE), a naturally occurring fluorescent sterol, was incorporated into cultured L-cel...

journal_title：Biochemistry

authors： Zhang W,McIntosh AL,Xu H,Wu D,Gruninger T,Atshaves B,Liu JC,Schroeder F

更新日期：2005-03-01 00:00:00

abstract：:The tertiary structure of alpha1-acid glycoprotein (AGP) remains unresolved despite its novel function because AGP is a hard target in X-ray and NMR analyses. To elucidate the membrane-induced conformational change of AGP, the vacuum-ultraviolet circular dichroism (VUVCD) spectra of AGP and its constituent sugars were...

journal_title：Biochemistry

authors： Matsuo K,Namatame H,Taniguchi M,Gekko K

更新日期：2009-09-29 00:00:00

abstract：:A member of the SF2 family of helicases, Escherichia coli RecQ, is involved in the recombination and repair of double-stranded DNA breaks and single-stranded DNA (ssDNA) gaps. Although the unwinding activity of this helicase has been studied biochemically, the mechanism of translocation remains unclear. To this end, u...

journal_title：Biochemistry

authors： Rad B,Kowalczykowski SC

更新日期：2012-04-03 00:00:00

abstract：:Calmodulin (CaM) is an essential, eukaryotic protein comprised of two highly homologous domains (N and C). CaM binds four calcium ions cooperatively, regulating a wide array of target proteins. A genetic screen of Paramecia by Kung [Kung, C. et al. (1992) Cell Calcium 13, 413-425] demonstrated that the domains of CaM ...

journal_title：Biochemistry

authors： VanScyoc WS,Newman RA,Sorensen BR,Shea MA

更新日期：2006-12-05 00:00:00

abstract：:The mechanism of wild-type and R37A mutant Pseudomonas dacunhae aspartate beta-decarboxylase (ABDC) was studied by rapid-scanning stopped-flow spectrophotometry. Mixing wild-type ABDC with 50 mM disodium l-Asp resulted in the formation of a 325 nm absorption peak within the dead time of the stopped-flow instrument, li...

journal_title：Biochemistry

authors： Phillips RS,Lima S,Khristoforov R,Sudararaju B

更新日期：2010-06-22 00:00:00

abstract：:(Z)-3-(Fluoromethyl)phosphoenolpyruvate has been synthesized in nine chemical steps from glyoxylic acid. The compound is stable at pH 3, but at pH 8 it decomposes within seconds to give 2-oxo-3-butenoate. When 3-(fluoromethyl)phosphoenolpyruvate is added to a solution of phosphoenolpyruvate carboxylase or pyruvate kin...

journal_title：Biochemistry

authors： Wirsching P,O'Leary MH

更新日期：1988-02-23 00:00:00

abstract：:Pseudomonas aeruginosa PA4872 was identified by sequence analysis as a structurally and functionally novel member of the PEP mutase/isocitrate lyase superfamily and therefore targeted for investigation. Substrate screens ruled out overlap with known catalytic functions of superfamily members. The crystal structure of ...

journal_title：Biochemistry

authors： Narayanan BC,Niu W,Han Y,Zou J,Mariano PS,Dunaway-Mariano D,Herzberg O

更新日期：2008-01-08 00:00:00

abstract：:Two paramagnetic probes, viz., Mn2+ and Cr3+-ATP, were used to map distances to various loci on carbamoyl-phosphate synthetase by using NMR measurements. The paramagnetic influence of Mn2+ on the 1H of L-glutamate and L-ornithine was measured at 200 and 360 MHz. On the basis of these data, a correlation time for the p...

journal_title：Biochemistry

authors： Raushel FM,Anderson PM,Villafranca JJ

更新日期：1983-04-12 00:00:00

abstract：:The melibiose permease of Salmonella typhimurium (MelB(St)) catalyzes symport of melibiose with Na(+), Li(+), or H(+), and bioinformatics analysis indicates that a conserved Gly117 (helix IV) is part of the Na(+)-binding site. We mutated Gly117 to Ala, Pro, Trp, or Arg; the effects on melibiose transport and binding o...

journal_title：Biochemistry

authors： Guan L,Jakkula SV,Hodkoff AA,Su Y

更新日期：2012-04-03 00:00:00

abstract：:The micellar properties of mixed, bovine gangliosides and purified galactosyl-N-acetylacetylgalactosaminyl (N-acetylneuraminyl) galactosylglucosylceramide were studied by gel filtration, equilibrium dialysis, and band and boundary centrifugation in sucrose gradients. The dissociation of micelles is very slow (days) in...

journal_title：Biochemistry

authors： Formisano S,Johnson ML,Lee G,Aloj SM,Edelhoch H

更新日期：1979-03-20 00:00:00

abstract：:It is known that, while melittin at micromolar concentrations is unfolded under conditions of low ionic strength at neutral pH, it adopts a tetrameric alpha-helical structure under conditions of high ionic strength, at alkaline pH, or at high peptide concentrations. To understand the mechanism of the conformational tr...

journal_title：Biochemistry

authors： Goto Y,Hagihara Y

更新日期：1992-01-28 00:00:00

abstract：:We examine the relationship between binding affinity and interface size for reversible protein-protein interactions (PPIs), using cytokines from the tumor necrosis factor (TNF) superfamily and their receptors as a test case. Using surface plasmon resonance, we measured single-site binding affinities for binding of the...

journal_title：Biochemistry

authors： Day ES,Cote SM,Whitty A

更新日期：2012-11-13 00:00:00

abstract：:Aminoimidazole riobnucleotide carboxylase, the sixth step in the purine biosynthetic pathway, catalyzes the conversion of aminoimidazole ribonucleotide (AIR) to carboxyaminoimidazole ribonucleotide (CAIR). The gene products of the purE and purK genes (PurE and PurK, respectively) thought to be responsible for this act...

journal_title：Biochemistry

authors： Meyer E,Leonard NJ,Bhat B,Stubbe J,Smith JM

更新日期：1992-06-02 00:00:00

abstract：:The aureolic acid antitumor antibiotic mithramycin (MTM) inhibits both cancer growth and bone resorption by cross-linking GC-rich DNA, thus blocking binding of Sp-family transcription factors to gene regulatory elements. Transcription of c-src, a gene implicated in many human cancers and required for osteoclast-depend...

journal_title：Biochemistry

authors： Remsing LL,Bahadori HR,Carbone GM,McGuffie EM,Catapano CV,Rohr J

更新日期：2003-07-15 00:00:00

abstract：:The substrate specificity of the human enzyme glutathione reductase was changed from its natural substrate glutathione to trypanothione [N1,N8-bis(glutathionyl)spermidine] by site-directed mutagenesis of two residues. The glutathione analogue, trypanothione, is the natural substrate for trypanothione reductase, an enz...

journal_title：Biochemistry

authors： Bradley M,Bücheler US,Walsh CT

更新日期：1991-06-25 00:00:00

abstract：:The first committed step of fatty acid and polyketides biosynthesis, the biotin-dependent carboxylation of an acyl-CoA, is catalyzed by acyl-CoA carboxylases (ACCases) such as acetyl-CoA carboxylase (ACC) and propionyl-CoA carboxylase (PCC). ACC and PCC in Streptomyces coelicolor are homologue multisubunit complexes t...

journal_title：Biochemistry

authors： Arabolaza A,Shillito ME,Lin TW,Diacovich L,Melgar M,Pham H,Amick D,Gramajo H,Tsai SC

更新日期：2010-08-31 00:00:00

abstract：:Although there is considerable evidence that signaling by the erythropoietin (EPO) receptor is initiated when it is dimerized by binding EPO, it has been previously reported that the soluble extracellular domains of the EPO receptor (sEPOR) are not dimerized in the presence of EPO and are able to form only 1:1 complex...

journal_title：Biochemistry

authors： Philo JS,Aoki KH,Arakawa T,Narhi LO,Wen J

更新日期：1996-02-06 00:00:00

abstract：:Comparative mutagenesis studies of N-(2'-deoxyguanosin-8-yl)-2-acetylaminofluorene (dG-AAF) and N-(2'-deoxyguanosin-8-yl)-2-aminofluorene (dG-AF) adducts positioned in the Nar I restriction enzyme site were performed using Escherichia coli (E. coli) and simian kidney (COS-7) cells. Oligodeoxynucleotides ((5)(')TCCTCG(...

journal_title：Biochemistry

authors： Tan X,Suzuki N,Grollman AP,Shibutani S

更新日期：2002-12-03 00:00:00

abstract：:Femtosecond spectroscopy was performed on CO-liganded (fully reduced and mixed-valence states) and O(2)-liganded quinol oxidase bd from Escherichia coli. Substantial polarization effects, unprecedented for optical studies of heme proteins, were observed in the CO photodissociation spectra, implying interactions betwee...

journal_title：Biochemistry

authors： Borisov VB,Liebl U,Rappaport F,Martin JL,Zhang J,Gennis RB,Konstantinov AA,Vos MH

更新日期：2002-02-05 00:00:00

abstract：:While two-component systems (TCSs), composed of a sensor histidine kinase (HK) and a response regulator, are the main signaling pathways in bacteria, global TCS activity remains poorly described. Here, we report the kinetic parameters of the HK autophosphorylation reaction using previously uncharacterized γ-phosphate-...

journal_title：Biochemistry

authors： Chase OM,Espinasse A,Wilke KE,Carlson EE

更新日期：2018-07-24 00:00:00

abstract：:Runx proteins constitute a family of mammalian transcription factors that interact with DNA through their evolutionarily conserved Runt domain. CBFbeta, alternatively denoted PEBP2beta, is the non-DNA-binding heterodimer partner and acts to enhance the DNA binding affinity of Runx proteins. Runx proteins and CBFbeta a...

journal_title：Biochemistry

authors： Wolf-Watz M,Grundström T,Härd T

更新日期：2001-09-25 00:00:00

abstract：:X-ray absorption spectra are reported for the multi-Cu oxidase Rhus vernicifera laccase in oxidized and fully reduced forms and for laccase from which the type 2 Cu has been depleted (T2D). The structure of the Cu K edge for both preparations shows the presence of CuII and CuI in the oxidized and reduced states, respe...

journal_title：Biochemistry

authors： Woolery GL,Powers L,Peisach J,Spiro TG

更新日期：1984-07-17 00:00:00

abstract：:In the following, molecular simulations are used to reveal unexpected behavior within bacterial membranes. We show that lipopolysaccharide molecules found in these membranes form viscous amorphous solids when they are interlinked with monovalent and divalent cations. The bilayers exhibit both liquid and glassy charact...

journal_title：Biochemistry

authors： Jefferies D,Hsu PC,Khalid S

更新日期：2017-03-21 00:00:00

abstract：:Thermodynamic parameters of helix formation were measured spectroscopically for 16 oligoribonucleotides containing either internal GU mismatches or the corresponding AU pairs. Internal GU mismatches stabilize each helix, but not as much as the corresponding AU pairs. The differences in the enthalpy and entropy changes...

journal_title：Biochemistry

authors： Sugimoto N,Kierzek R,Freier SM,Turner DH

更新日期：1986-09-23 00:00:00

abstract：:An affinity labeling technique has been developed to identify the folate-methotrexate transporter of Leishmania donovani promastigotes using "activated" derivatives of the ligands. These "activated" derivatives were synthesized by incubating folate and methotrexate with a 10-fold excess of 1-ethyl-3-[3-(dimethylamino)...

journal_title：Biochemistry

authors： Beck JT,Ullman B

更新日期：1989-08-22 00:00:00

abstract：:Fibronectin (FN) is an extracellular matrix (ECM) protein found soluble in corporal fluids or as an insoluble fibrillar component incorporated in the ECM. This phenomenon implicates structural changes that expose FN binding sites and activate the protein to promote intermolecular interactions with other FN. We have in...

journal_title：Biochemistry

authors： Patel S,Chaffotte AF,Goubard F,Pauthe E

更新日期：2004-02-17 00:00:00

abstract：:We have obtained the 470 MHz 19F NMR spectra of wild type [4-F]Trp-labeled myoglobins (MbCO, MbO2, deoxyMb, metMb, and MbCN) and hemoglobins (HbCO, HbO2, and deoxyHb), as well as those of several mutants (W7F Mb, betaW15F Hb, betaW37S Hb, and betaY130F Hb, all as the carbonmonoxy adducts), prepared via site-directed m...

journal_title：Biochemistry

authors： Pearson JG,Montez B,Le H,Oldfield E,Chien EY,Sligar SG

更新日期：1997-03-25 00:00:00

abstract：:Two major neutral glyceroglucolipids (A and B) have been isolated from lipid extract of human gastric content by the procedure involving column fractionation on DEAE-Sephadex, silicic acid, and thin-layer chromatography. Both glycolipids contained glucose, glyceryl ethers, and fatty acids. The structures of these glyc...

journal_title：Biochemistry

authors： Slomiany BL,Slomiany A,Glass GB

更新日期：1977-09-06 00:00:00