Abstract：
:A preparation of small single-walled liposome vesicles containing a 9:1 mole ratio of phosphatidylcholine to phosphatidylethanolamine was subjected to reaction with the membrane-impermeable reagent, isethionyl acetimidate hydrochloride. This reagent converted 90% of the external phosphatidylethanolamine groups to the amidine derivative, leaving the mole ratio of unreacted phosphatidylethanolamine to phosphatidylcholine on the outside surface of the vesicle much lower than that on the inside surface. Equilibration of phosphatidylethanolamine across the bilayer was then measured as a function of time by monitoring the appearance of phosphatidylethanolamine on the outside surface utilizing the reaction of the amino groups with 2, 4, 6-trinitrobenzenesulfonic acid. The results show that no new phosphatidylethanolamine appeared on the external surface of the vesicles over a period of 12 days at 22 degrees. A conservative estimate of the precision of the measurements is +/- 10%. On this basis, the estimated half-time for the equilibration of phosphatidylethanolamine across the bilayer of these vesicles must be at least 80 days at 22 degrees.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Roseman M,Litman BJ,Thompson TEdoi
10.1021/bi00693a008subject
Has Abstractpub_date
1975-11-04 00:00:00pages
4826-30issue
22eissn
0006-2960issn
1520-4995journal_volume
14pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The major plasma membrane protein of human erythrocytes is the anion transport protein, termed protein 3. We previously reported a variant form of protein 3 that is elongated on the amino-terminal end of the molecule, which is exposed on the cytoplasmic side of the membrane, but otherwise its features are identical wi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00334a003
更新日期：1985-06-18 00:00:00
abstract：:Base excision repair (BER) is the major corrective pathway for most spontaneous, oxidative, and alkylation DNA base and sugar damage. X-ray cross-complementing 1 (XRCC1) has been suggested to function at nearly every step of this repair process, primarily through direct protein-protein interactions. Using whole cell e...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi051161o
更新日期：2005-11-01 00:00:00
abstract：:High signal-to-noise Fourier transform infrared (FTIR) spectra of the 5-hydroxytryptamine (serotonin) receptor (5-HT(3)R) and the nicotinic acetylcholine receptor (nAChR) were obtained by microscope FTIR spectroscopy using micrometer-sized, fully hydrated protein films. Because this novel procedure requires only nanog...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi035113k
更新日期：2003-12-02 00:00:00
abstract：:P-Glycoprotein (Pgp) expression in cell lines derived from tumors arising from cells which normally express Pgp can be increased by sodium butyrate and other differentiating agents. Although the Pgp level increased 25-fold after sodium butyrate treatment in SW620 human colon carcinoma cells, the intracellular accumula...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00143a002
更新日期：1992-07-21 00:00:00
abstract：:FeNO vibrational frequencies were investigated for a series of myoglobin mutants using isotope-edited resonance Raman spectra of (15/14)NO adducts, which reveal the FeNO and NO stretching modes. The latter give rise to doublet bands, as a result of Fermi resonances with coincident porphyrin vibrations; these doublets ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi026395b
更新日期：2003-05-06 00:00:00
abstract：:The common reactions of dioxygen, superoxide, and hydroperoxides with thiolates are thought to proceed via persulfenate intermediates, yet these have never been visualized. Here we report a 1.4 A resolution crystal structure of the Fe(2+)-dependent enzyme cysteine dioxygenase (CDO) containing this putative intermediat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi801546n
更新日期：2008-11-04 00:00:00
abstract：:The removal of damaged bases by DNA glycosylases is thought to be effectively irreversible, because of an overall equilibrium that favors hydrolysis over synthesis of the N-glycosyl bond. Surprisingly, human alkyladenine DNA glycosylase (AAG) can make damaged DNA by catalyzing formation of an N-glycosyl bond between 1...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi101380d
更新日期：2010-10-26 00:00:00
abstract：:DrrA and DrrB proteins form an ATP-dependent efflux pump for doxorubicin and daunorubicin in Streptomyces peucetius. DrrA, the catalytic subunit, forms a complex with the integral membrane protein DrrB. Previous studies have provided evidence for strong interaction between these two proteins, which was found to be cri...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi701699a
更新日期：2008-03-04 00:00:00
abstract：:When they are translated, steroid receptors are assembled into a multiprotein complex containing hsp90, p23, an immunophilin, and often some hsp70. Some of the receptors, such as that for progesterone, have nuclear localization signals that are functional in the absence of hormone, and they move into the nucleus where...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi970648x
更新日期：1997-06-24 00:00:00
abstract：:It is generally accepted that electron transfer in bacterial photosynthesis is driven by the first singlet excited state of a special pair of bacteriochlorophylls (P*). We have examined the first steps of electron transfer in a mutant of the Rhodobacter sphaeroides reaction center in which charge separation from P* is...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9703756
更新日期：1997-06-10 00:00:00
abstract：:Despite the high degree of sequence similarity in their basic-helix-loop-helix (BHLH) domains, MASH-1 and MyoD are involved in different biological processes. In order to define possible differences between the DNA binding specificities of these two proteins, we investigated the DNA binding properties of MASH-1 by cir...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00035a008
更新日期：1995-09-05 00:00:00
abstract：:Enterokinase has been isolated from the contents of bovine duodena and purified 1200-fold in 41% yield. The isolation procedure employed DEAE chromatography, affinity chromatography on immobilized p-aminobenzamidine, and gel filtration. The resultant pure enzyme exhibits a single band on sodium dodecyl sulfate gel ele...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00634a011
更新日期：1977-07-26 00:00:00
abstract：:Plasma membrane and vesicular transporters for the biogenic amines, dopamine, norepinephrine, and serotonin, represent a group of proteins that play a crucial role in the regulation of neurotransmission. Clinically, mono amine transporters are the primary targets for the actions of many therapeutic agents used to trea...
journal_title：Biochemistry
pub_type： 杂志文章,评审
doi：10.1021/bi200405c
更新日期：2011-08-30 00:00:00
abstract：:The effect of pretreatment with sodium butyrate on DNA excision repair was studied in intact and permeable confluent (i.e., growth-inhibited) diploid human fibroblasts. Exposure to 20 mM sodium butyrate for 48 h increased subsequent ultraviolet (UV)-induced [methyl-3H]thymidine incorporation by intact AG1518 fibroblas...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00345a019
更新日期：1985-11-19 00:00:00
abstract：:Site-directed mutagenesis was used to construct mutant Trp repressors with each of the 38 possible single amino acid changes of the first 2 amino acid residues (Ile79 and Ala80) in the second "recognition" alpha-helix of the helix-turn-helix DNA-binding motif. Eight of these mutant repressors with Ile79 and Ala80 chan...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00203a038
更新日期：1994-09-20 00:00:00
abstract：:The early steps of the proposed mechanistic pathway for dehydroquinate synthase have been probed with a series of substrate analogues. These analogues, 3-9, are structurally prohibited from undergoing the beta-elimination of inorganic phosphate that represents the committed step in the conversion of the substrate 3-de...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00445a010
更新日期：1989-09-19 00:00:00
abstract：:We have recently reported that the Na pump alpha-subunit has cytosolic-oriented oligosaccharides which were sensitive to cleavage by an enzyme specific for hydrolysis of N-linked glycans [Pedemonte et al. (1990) Proc. Natl. Acad. Sci. U.S.A. 87, 9789-9793]. We now describe experiments that characterize the saccharides...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00158a008
更新日期：1992-11-03 00:00:00
abstract：:The interaction of the 20-kDa pore-forming domain of colicin A with phospholipid vesicles was investigated by gel permeation chromatography, analytical centrifugation, and electron microscopy. Under the experimental conditions of this study, this peptide was found to interact only with vesicles containing negatively c...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00445a029
更新日期：1989-09-19 00:00:00
abstract：:Light chain amyloidoses arise from the overproduction and abnormal deposition of the immunoglobulin light chain in various organs. LEN is the variable domain of an immunoglobulin light chain originally isolated from the urine of a patient suffering from multiple myeloma, with no sign of renal dysfunction or amyloidosi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi800806d
更新日期：2008-08-19 00:00:00
abstract：:Proteins from the enhanced intracellular survival (Eis) family are versatile acetyltransferases that acetylate amines at multiple positions of several aminoglycosides (AGs). Their upregulation confers drug resistance. Homologues of Eis are present in diverse bacteria, including many pathogens. Eis from Mycobacterium t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00244
更新日期：2015-05-26 00:00:00
abstract：:In the dark, 4-azido-2-nitrophenyl phosphate (ANPP) is a phosphate analog which behaves like a simple energy-rich phosphate donor for the sarcoplasmic reticulum (SR) Ca-ATPase. Like p-nitrophenyl phosphate (pNPP), ANPP is hydrolyzed by the enzyme only in the presence of calcium and magnesium (K0.5 and Vmax are 0.3 mM ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00175a030
更新日期：1994-03-08 00:00:00
abstract：:The epidermal growth factor receptor (EGFR) kinase is activated by a variety of mutations in human cancers. R776H is one such recurrent mutation (R752H in another numbering system) in the αC-β4 loop of the tyrosine kinase domain that activates EGFR in the absence of the activating EGF ligand. However, the mechanistic ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00444
更新日期：2015-07-14 00:00:00
abstract：:In reticulocytes, a single ribosomal protein, S13, has been shown to be phosphorylated by the cAMP-regulated protein kinases. The 40S ribosomal subunits were phosphorylated in vitro with [gamma-32P]ATP to facilitate the identification of S13 during the two-step purification procedure. Total ribosomal protein from the ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00604a003
更新日期：1978-05-30 00:00:00
abstract：:The mitogen-activated protein kinases (MAPKs) are key components of cellular signal transduction pathways, which are down-regulated by the MAPK phosphatases (MKPs). Catalytic activity of the MKPs is controlled both by their ability to recognize selective MAPKs and by allosteric activation upon binding to MAPK substrat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.7b00827
更新日期：2017-11-21 00:00:00
abstract：:VSTx1 is a voltage sensor toxin from the spider Grammostola spatulata that inhibits KvAP, an archeabacterial voltage-activated K(+) channel whose X-ray structure has been reported. Although the receptor for VSTx1 and the mechanism of inhibition are unknown, the sequence of the toxin is related to hanatoxin (HaTx) and ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0477034
更新日期：2005-04-26 00:00:00
abstract：:The binding site of cyclosporin A to P-glycoprotein was characterized by using a multidrug-resistant Chinese hamster ovary cell line. P-glycoprotein photolabeled with diazirine-cyclosporin A analogue was purified by a two-step process involving continuous elution electrophoresis followed by wheat germ agglutinin-agaro...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi981992c
更新日期：1998-12-22 00:00:00
abstract：:Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of d-glyceraldehyde 3-phosphate (GAP) into d-glycerate 1,3-bisphosphate (1,3-diPG) in the presence of NAD(+) and inorganic phosphate (P(i)). Within the active site, two anion-binding sites were ascribed to the binding o...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0206107
更新日期：2003-06-17 00:00:00
abstract：:The hammerhead ribozyme undergoes a well-defined two-stage conformational folding process, induced by the binding of magnesium ions. In this study, we have used isothermal titration calorimetry to analyze the thermodynamics of magnesium binding and magnesium ion-induced folding of the ribozyme. Binding to the natural ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi002231o
更新日期：2001-02-06 00:00:00
abstract：:The N-acyl- l-homoserine lactone hydrolases (AHL lactonases) have attracted considerable attention because of their ability to quench AHL-mediated quorum-sensing pathways in Gram-negative bacteria and because of their relation to other enzymes in the metallo-beta-lactamase superfamily. To elucidate the detailed cataly...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi8003704
更新日期：2008-07-22 00:00:00
abstract：:The full three-dimensional structure of the catalytic domain of human stromelysin-1 (SCD) complexed to a novel and potent, nonpeptidic inhibitor has been determined by nuclear magnetic resonance spectroscopy (NMR). To accurately mimic assay conditions, the structure was obtained in Tris buffer at pH 6.8 and without th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi981328w
更新日期：1998-10-06 00:00:00
